LOCUS       pML85_pDONR-ZmIAA16     3420 bp ds-DNA     circular     10-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     primer          674..697
                     /label="ZmIAA16_fwd"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGGGAGACGCAGCAGAGACCAAC"
     misc_feature    674..1540
                     /label="ZmIAA16"
     CDS             674..1540
                     /label="Translation 674-1540"
     primer          complement(1509..1540)
                     /label="ZmIAA16_STOP_rev"
                     /note="sequence: AGAAAGCTGGGTGTCAAGAGGTAAACACTGGACACTCAGTTACCGTCC"
     misc_feature    1541..1543
                     /label="IAA16"
     CDS             1541..1543
                     /label="Translation 1541-1543"
     misc_feature    complement(1541..1556)
                     /label="attB2_tail+STOP"
     misc_feature    1544..1643
                     /label="L2"
     misc_recomb     complement(1548..1568)
                     /label="attB2"
     primer_bind     complement(1661..1682)
                     /label="T7"
     promoter        complement(1662..1680)
                     /label="T7_promoter"
     misc_feature    complement(1681..1699)
                     /label="M13_reverse_primer"
     CDS             1814..2623
                     /label="KanR2"
     gene            1814..2623
                     /label="KanR2"
     rep_origin      2754..3373
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGGGAG ACGCAGCAGA GACCAACAAG ACTCTTCACA ACTGGATGGG
      721 CGAGCCGAGG CCGAGGTCGA GCGACCAGCG CGACGAGGAG GAGACGCTTC AGCTCAGCCT
      781 CGGCCTCCCC GGAGGAGGAG CAAGTGCGGG TGGTGGGGTA GCACCCGCCG CCTGGAGAAT
      841 GCTGCCGGCC AGAGACGAGG AGATGCGCTC TGCTGCTGCT GTCGTCGACA CCTCCCTGCT
      901 CTCTCTCGGC TACTCCGCCC CAGCTTTCTC GCCCCGCTCA CCAGGCAAGG CAAAGGGGTC
      961 CCCAGCAGCA GCTACAGAGA ATGCCCTTGC ATCCACCAAC AACGCCTCCC AGAGAAGATC
     1021 TCCAAATACC CCGGTTATTG GGTGGCCTCC AGTTCGTGCC TTCAGAAGAA ATCTGGCCAC
     1081 CTCTAGCAGA GCATCCCTTG AACATCAGAA TGGGAAGAAG GAAGACAAAC CTGAACAGAC
     1141 CACAAAAAGA GCTCCATTTG TAAAGATCAA CATGGACGGC ATCCCTATTG GTAGAAAAAT
     1201 CGATCTGAGT GCCCTCGGTA GTTACGATGA GCTGTCTCTG TCTGTCGACA AGCTATTCCG
     1261 GGGGCTTCTT GCAGCACAAC AAGACCCTCT GGCTGCCGGC GCAAAGGAGT GTTCACAGGA
     1321 AGAGGTGGCG ATATCGGGTT TACTAGATGG AACTGGGGAG TACACTCTGG TTTATGAGGA
     1381 TTATGAAGGC GATAGGGTGC TGGTTGGCGA TGTCCCCTGG GGGATGTTTG TTTCTTCGGT
     1441 GAAGAGGCTG CGCGTTCTGA AGACATCTGA CCTCTCTTCA TCTCTAACAG CACCTCCTGG
     1501 CCAGAAGAGG ACGGTAACTG AGTGTCCAGT GTTTACCTCT TGACACCCAG CTTTCTTGTA
     1561 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT
     1621 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA
     1681 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT
     1741 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA
     1801 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA
     1861 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC
     1921 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA
     1981 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT
     2041 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC
     2101 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA
     2161 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA
     2221 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA
     2281 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT
     2341 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA
     2401 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG
     2461 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA
     2521 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT
     2581 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG
     2641 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA
     2701 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     2761 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     2821 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     2881 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG
     2941 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     3001 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     3061 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     3121 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
     3181 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     3241 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     3301 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     3361 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//