LOCUS       pML94_pDONR-ZmIAA21     3285 bp ds-DNA     circular     10-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     primer          674..694
                     /label="ZmIAA21_fwd"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGTCGCCCCCACTCGACCTC"
     misc_feature    674..1408
                     /label="ZmIAA21 (recloned)"
     CDS             674..1408
                     /label="Translation 674-1408"
     primer          complement(1377..1408)
                     /label="ZmIAA21_STOP_R_new"
                     /note="sequence: AGAAAGCTGGGTGCTAGTTGCGATTCTTGGACTTATCAGCTGCCC"
     misc_feature    complement(1409..1421)
                     /label="attB2_tail+STOP"
     misc_feature    1409..1508
                     /label="L2"
     misc_recomb     complement(1413..1433)
                     /label="attB2"
     primer_bind     complement(1526..1547)
                     /label="T7"
     promoter        complement(1527..1545)
                     /label="T7_promoter"
     misc_feature    complement(1546..1564)
                     /label="M13_reverse_primer"
     CDS             1679..2488
                     /label="KanR2"
     rep_origin      2619..3238
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGTCGC CCCCACTCGA CCTCGACTAC ATAGGCCTCT CGCCGGCGGC
      721 GGCTGCCGCC GCTGCCCACG ACGACCTGAA GGGTACCGAG CTCCGCCTTG GTCTGCCGGG
      781 GTCCGGTTCG CCGGACCGCC GTGTTGTGGC TGCCACCGCA ACCACCCTGG ACCTGCTCCC
      841 GGCTAAGGGC GCCAAGCGCG GGTTTTCTGA CGAGGCGCCG ACGCCATCGC CCGGTGCAGC
      901 TTCCGGGAAG GGAAAGAAGG TAGCGGAAGA GGAGGACGAC AAGAAGGTTG CAGCGACGCC
      961 GCAGCCGGTC GCAAAAGCTC AGGTGGTGGG ATGGCCACCA ATCCGAAGCT ACCGCAAGAA
     1021 CACGATGTCT ACCACCCAGC TGAAGGGCAG CAAGGAGGAT GCTGAGGCCA AGCAGGACCA
     1081 GGGGTTCCTG TACGTCAAGG TCAGCATGGA TGGCGCGCCG TACCTCAGGA AGATTGACCT
     1141 CAAGACTTAC AAGAACTACA AGGACCTGTC GACTGCGCTT GAGAAGATGT TCAGTGGCTT
     1201 CAGTACTGGC AAGGATGGCT TATCTGAGTA CCGCAAGGAT GGTGAATATG TTCTGACTTA
     1261 CGAGGACAAG GATGGAGATT GGATGCTTGT TGGCGATGTA CCATGGGAGA TGTTCGCTGA
     1321 CTCTTGCCGC AGGCTCAGGA TCATGAAAGG ATCAGATGCA ATTGGACTTG CTCCAAGGGC
     1381 AGCTGATAAG TCCAAGAATC GCAACTAGCA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
     1441 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
     1501 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
     1561 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT
     1621 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
     1681 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
     1741 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
     1801 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
     1861 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
     1921 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
     1981 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
     2041 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
     2101 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
     2161 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
     2221 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
     2281 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
     2341 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
     2401 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
     2461 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
     2521 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
     2581 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     2641 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     2701 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     2761 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     2821 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     2881 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     2941 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     3001 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     3061 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     3121 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     3181 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     3241 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//