LOCUS       pML95_pDONR-ZmIAA25     3159 bp ds-DNA     circular     10-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     misc_feature    674..1282
                     /label="ZmIAA25 (recloned)"
     CDS             674..1282
                     /label="Translation 674-1282"
     primer          675..694
                     /label="ZmIAA25_F_new"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGGAGCTCGACGCCGACAAC"
     primer          complement(1261..1282)
                     /label="ZmIAA25_STOP_R_new"
                     /note="sequence: AGAAAGCTGGGTGTCACCTGGCCTCAGAGGCAGAG"
     misc_feature    complement(1283..1295)
                     /label="attB2_tail+STOP"
     misc_feature    1283..1382
                     /label="L2"
     misc_recomb     complement(1287..1307)
                     /label="attB2"
     primer_bind     complement(1400..1421)
                     /label="T7"
     promoter        complement(1401..1419)
                     /label="T7_promoter"
     misc_feature    complement(1420..1438)
                     /label="M13_reverse_primer"
     CDS             1553..2362
                     /label="KanR2"
     gene            1553..2362
                     /label="KanR2"
     rep_origin      2493..3112
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGGAGC TCGACGCCGA CAACCTGAGC GCCACCGAGC TCCGCCTCGG
      721 CCTGCCTGGG ACCAGCAGCA GCAGCAGCGA CGACTGGCAG AAGAAGCCGT CGCCCTCCGT
      781 CGGGGCCAAG CGCGCTCTGG ACGACGGCAC CAAGAGCGAG GCCTCGGGGA CCAGCCCGGC
      841 CGCCAGAGAC CTCGACTTCG ACCACGACCA CGACACCGCC GCGCCTCCCA AGGCGCAAGT
      901 GGTCGGGTGG CCGCCGGTGA GGGCGTACAG GAAGAACACC TTCCAGGCGG CGGCGGCGGC
      961 CAAGAAGGTC GAGCAGAAGC AGAAGCAGCA GCAGCAGGGA GGCCTGTACG TGAAGGTGAG
     1021 CATGGACGGC GCGCCGTACC TCAGGAAGGT GGACCTCCGG ATGTACAAGG GGTACAGGGA
     1081 GCTCAGGGAG GCTCTGGACG CGCTCTTCAC CAACTCCTTC TCCGCGGCGG CAGAGGGCGG
     1141 CGGCGACCAC CACCAGCACG CCATCGCCTA CGAAGACAAG GACGGCGACC TCATGCTCGC
     1201 CGGAGACGTG CCCTGGGATA TGTTCATCTC TTCGTGCAAG AAGCTCAGGA TAATGAAGGG
     1261 CTCTGCCTCT GAGGCCAGGT GACACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA
     1321 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT
     1381 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG
     1441 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC
     1501 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA
     1561 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG
     1621 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG
     1681 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT
     1741 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA
     1801 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC
     1861 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC
     1921 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG
     1981 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA
     2041 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT
     2101 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT
     2161 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG
     2221 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA
     2281 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT
     2341 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT
     2401 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC
     2461 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     2521 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     2581 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     2641 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     2701 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     2761 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     2821 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     2881 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     2941 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     3001 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     3061 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     3121 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT
//