LOCUS       pML70_pDONR-ZmIAA28     3291 bp ds-DNA     circular     24-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     primer          674..695
                     /label="ZmIAA28_fwd"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGTCGCCTCCACTCGAGCTCG"
     misc_feature    674..1411
                     /label="ZmIAA28"
     CDS             674..1414
                     /label="Translation 674-1414"
     primer          complement(1386..1411)
                     /label="ZmIAA28_rev"
                     /note="sequence: AGAAAGCTGGGTGTCAGTTGCGGTTCTTGGACTTATCGGCTG"
     misc_feature    1412..1414
                     /label="STOP"
     misc_feature    complement(1412..1427)
                     /label="attB2_tail+STOP"
     misc_feature    1412..1514
                     /label="L2"
     misc_recomb     complement(1419..1439)
                     /label="attB2"
     primer_bind     complement(1532..1553)
                     /label="T7"
     promoter        complement(1533..1551)
                     /label="T7_promoter"
     misc_feature    complement(1552..1570)
                     /label="M13_reverse_primer"
     gene            1685..2494
                     /label="KanR2"
     rep_origin      2625..3244
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGTCGC CTCCACTCGA GCTCGACTAC ATAGGCCTCT CGCCGCCGGC
      721 GGCAGCCGCC GCCGCTGAAA ACGACGAACT GAAGGGTACT GAGCTCCGCC TCGGGCTGCC
      781 AGGGTCCGGG TCGCCGGACC GCCGTGTCGT GGCTGCCACC GCGACCACCC TGGACCTGCT
      841 CCCGGCCAAG GGCGCCAAGC GCGGGTTTTC TGACGAGGCG CCCCCGCCAT CGCCTGTTGC
      901 CACTGCCGGA AAGGGAAAGA AGGTGGCAGA AGAGGAGTAC GACGAGAAGA AGGTGGCAGC
      961 GACCCCGCAG CCGGCCGCCA AAGCACAGGT GGTGGGATGG CCACCAGTCT GCAACTACCG
     1021 CAAGAACACG ATGACTACCA CCCAGCTGGA GGGCAGCAAG GAGGATGGTG ATGCCAAGCA
     1081 GGGTCAGGGG TTCCTGTACG TCAAGGTCAG CATGGATGGC GCGCCGTACC TCAGGAAGAT
     1141 CGACCTCAAG ACTTACAAGA ACTACAAGGA CCTGTCGACC GCGCTTGAGA AGATGTTCAG
     1201 TGGCTTCAGT ACTGGCAAGG ACGGCTCATC TGAGTACCGC AAGGATGGTG AATATGTTCT
     1261 GACTTACGAG GACAAGGATG GAGATTGGAT GCTTGTCGGC GACGTACCAT GGGAGATGTT
     1321 CGCCGGCTCC TGCCGCAGGC TCAGGATCAT GAAAGGCTCA GATGCGATTG GACTCGCTCC
     1381 AAGGGCAGCC GATAAGTCCA AGAACCGCAA CTGACACCCA GCTTTCTTGT ACAAAGTTGG
     1441 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA
     1501 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT
     1561 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA
     1621 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG
     1681 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC
     1741 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA
     1801 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT
     1861 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT
     1921 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT
     1981 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT
     2041 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT
     2101 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT
     2161 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA
     2221 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT
     2281 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG
     2341 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC
     2401 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT
     2461 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA
     2521 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA
     2581 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     2641 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     2701 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     2761 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     2821 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     2881 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     2941 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     3001 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     3061 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     3121 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     3181 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     3241 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T
//