LOCUS       pML131_pDONR-BIF1-STOP- 3111 bp ds-DNA     circular     10-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      293..912
                     /label="pBR322_origin"
     terminator      complement(1227..1254)
                     /label="rrnB_T2_terminator"
     terminator      complement(1386..1429)
                     /label="rrnB_T1_terminator"
     misc_feature    1481..1503
                     /label="M13_pUC_fwd_primer"
     misc_feature    1496..1512
                     /label="M13_forward20_primer"
     misc_feature    1528..1627
                     /label="L1"
     misc_recomb     1604..1624
                     /label="attB1"
     misc_feature    1616..1632
                     /label="attB1_adaptor_tail"
     misc_feature    1628..1629
                     /label="TC In Frame"
     misc_feature    1630..1632
                     /label="Yeast Kozak"
     misc_feature    1633..2190
                     /label="BIF1"
     CDS             1633..2190
                     /label="Translation 1633-2190"
     misc_feature    2191..2193
                     /label="STOP"
     CDS             2191..2193
                     /label="Translation 2191-2193"
     misc_feature    complement(2194..2206)
                     /label="attB2_tail+STOP"
     misc_feature    2194..2293
                     /label="L2"
     misc_recomb     complement(2198..2218)
                     /label="attB2"
     primer_bind     complement(2311..2332)
                     /label="T7"
     promoter        complement(2312..2330)
                     /label="T7_promoter"
     misc_feature    complement(2331..2349)
                     /label="M13_reverse_primer"
     CDS             2464..162
                     /label="KanR2"
ORIGIN
        1 CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG
       61 TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG
      121 AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG
      181 TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA
      241 TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
      301 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
      361 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
      421 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC
      481 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
      541 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
      601 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
      661 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT
      721 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
      781 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
      841 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
      901 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
      961 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT
     1021 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG
     1081 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
     1141 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATAC GCGTACCGCT
     1201 AGCCAGGAAG AGTTTGTAGA AACGCAAAAA GGCCATCCGT CAGGATGGCC TTCTGCTTAG
     1261 TTTGATGCCT GGCAGTTTAT GGCGGGCGTC CTGCCCGCCA CCCTCCGGGC CGTTGCTTCA
     1321 CAACGTTCAA ATCCGCTCCC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC
     1381 AACAGATAAA ACGAAAGGCC CAGTCTTCCG ACTGAGCCTT TCGTTTTATT TGATGCCTGG
     1441 CAGTTCCCTA CTCTCGCGTT AACGCTAGCA TGGATGTTTT CCCAGTCACG ACGTTGTAAA
     1501 ACGACGGCCA GTCTTAAGCT CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC
     1561 TGTTCGTTGC AACAAATTGA TGAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA
     1621 GCAGGCTTCA AAATGTCGGT GGATACGGAG CGGAGCTCCA CCGAGTCGTC CGCGGCCTCC
     1681 GGGCTCGGCT ACGAGGACAC CGCGCTCGCC CTCACCCTCC GCCTCCCGGG CTCCGACCCC
     1741 GGCCGTTCCT CCCCGCTCGC CGCGCCCTCC GACGCCGCGC CGTCTCCCAA GACGCGGGTG
     1801 GTGGGGTGGC CGCCGGTGAG GTCGTACCGC AAGAACGCGC TCGCCGACTC CAGCAAGGCC
     1861 AACCGGTCAG CCAGTTTCGT GAAGGTGGCC GTCGACGGCG CGGCCTACCT GCGGAAGGTG
     1921 GACCTCCAGG CGTACGGCGG CTACGACCAG CTCCTCCGCG CGCTCCAGGA CAAGTTCTTC
     1981 TCCCACTTCA CCATCAGGAA GTTCGCCGAC GACGAGAGGA AGCTGGTGGA CGCGGTGAAC
     2041 GGGACGGAGT ACGTGCCCAC GTACGAGGAC AAGGATGGCG ACTGGATGCT CGTCGGCGAC
     2101 GTCCCCTGGA AGATGTTCGT GGAGACCTGC CGGCGCCTTC GCCTGATGAA AGGTTCAGAG
     2161 GCCGTTAACT TGGCACCAAG AGCCGCCCGA TGACACCCAG CTTTCTTGTA CAAAGTTGGC
     2221 ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT
     2281 AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA
     2341 GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT
     2401 AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT
     2461 GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT
     2521 GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT
     2581 CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT
     2641 GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT
     2701 CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC
     2761 CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT
     2821 GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT
     2881 AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT
     2941 GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA
     3001 ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT
     3061 GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG A
//