LOCUS       pML130_pDONR-BIF4-STOP- 3255 bp ds-DNA     circular     24-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     misc_feature    674..1375
                     /label="BIF4"
     CDS             674..1375
                     /label="Translation 674-1375"
     misc_feature    1376..1378
                     /label="STOP"
     CDS             1376..1378
                     /label="Translation 1376-1378"
     misc_feature    complement(1379..1391)
                     /label="attB2_tail+STOP"
     misc_feature    1379..1478
                     /label="L2"
     misc_recomb     complement(1383..1403)
                     /label="attB2"
     primer_bind     complement(1496..1517)
                     /label="T7"
     promoter        complement(1497..1515)
                     /label="T7_promoter"
     misc_feature    complement(1516..1534)
                     /label="M13_reverse_primer"
     CDS             1649..2458
                     /label="KanR2"
     rep_origin      2589..3208
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGGCTT GGAATGGCCG CTTCGGCGAC GACGAGGAGG GGAGCGGCCT
      721 TGAGCTCAGC CTTAGCCTCC CGGGCTACTT CTCCGGATCG CCAACCCAAG CTGCAGCAGG
      781 TTTCGAGGAC AAAGCAGACG GCGGCTCTGC TGCTGCTGCT GCTGCTGGGC GAGCAAAGGG
      841 AAGCAATGGC TTCAAGGCCA GGCCGGCGGC AGCTGCTCCG GTCGTGGGAT GGCCGCCAGT
      901 GCGCGCGTTC CGGCGGAACC TCGCCTCGGC GTCCTCCAAG CCGTCCCGCG ATCCGCCACC
      961 AAGCCACAGA GGAAACGAGT CCGCGAGCGT CGGCGCCGGC GGCAAGGCCG TCGAGGGCGG
     1021 CAACAAGGGC CTGTTCGTCA AGGTCAACAT GGACGGCGTG CCCATCGGCC GGAAGCTCGA
     1081 CCTGGGCGGC CACGCCGGCT ACGACACGCT AACCGCCGCC GTCGACCACC TCTTCCGCGG
     1141 CCTCCTCTCC GCTCAAACGT CGGGACCAGG CGGCGAGCGG CAGGCGGTCG CGGGGATCCT
     1201 GAACGGTGGC GGCGAGTACA CGCTGGTGTA CGAGGACGAC GAGGGAGACC AGATGCTGGT
     1261 CGGGGACGTG CCGTGGCAGA TGTTCGCCGC CACCGCCAGG CGTCTGCGCG TGCTGCGGAG
     1321 CTCCGATCTC AACCCCTCGT CGCTAAGGGC AGCGACCAGG AAGAGAGCTG CAGCTTGACA
     1381 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA
     1441 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA
     1501 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA
     1561 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC
     1621 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
     1681 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
     1741 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
     1801 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
     1861 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
     1921 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
     1981 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
     2041 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
     2101 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
     2161 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
     2221 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
     2281 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
     2341 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
     2401 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC
     2461 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC
     2521 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC
     2581 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     2641 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     2701 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     2761 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     2821 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     2881 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     2941 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     3001 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     3061 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     3121 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     3181 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     3241 TTTTGCTCAC ATGTT
//