LOCUS       HisFLAGTev_ACTB_in_pJC  5794 bp ds-DNA     circular     11-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..589
                     /label="CMV"
     misc_feature    554..560
                     /label="TATA box"
     misc_feature    583..584
                     /label="Transcription start point"
     misc_feature    592..603
                     /label="MCS"
     CDS             615..1817
                     /label="Translation 615-1817"
     misc_feature    621..638
                     /label="6xHis"
     misc_feature    645..668
                     /label="FLAG"
     misc_feature    675..692
                     /label="TEV"
     misc_feature    693..1814
                     /label="betaActin"
     misc_feature    1826..1831
                     /label="Make pst1 site?"
     misc_feature    1954..1969
                     /label="Filler MCS_KO"
     enhancer        1976..2567
                     /label="WPRE"
     misc_feature    2613..2663
                     /label="SV40 early mRNA polyA signal"
     misc_feature    2710..3165
                     /label="f1"
     misc_feature    3227..3232
                     /label="-35 region"
     misc_feature    3227..3255
                     /label="Bacterial promoter for KanR"
     misc_feature    3250..3255
                     /label="-10 region"
     misc_feature    3339..3607
                     /label="SV40"
     misc_feature    3506..3641
                     /label="SV40 oriR"
     misc_feature    3690..4484
                     /label="KanR"
     misc_feature    4720..4738
                     /label="Herpes simplex virus (HSV) polyA"
     misc_feature    5069..5712
                     /label="pUC"
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCGC
      601 GAGTCGACGC CACCATGGCG catcaccatc atcaccacAG TGGAGATTAC AAGGATGACG
      661 ATGACAAGAC TAGTgagaac ctgtatttcc agGACGATGA TATTGCTGCT CTGGTTGTCG
      721 ACAACGGCTC TGGCATGTGC AAGGCCGGAT TTGCCGGAGA CGATGCTCCT CGCGCCGTCT
      781 TCCCATCGAT TGTGGGACGT CCCCGTCACC AGGGTGTGAT GGTCGGCATG GGCCAGAAGG
      841 ACTCGTACGT GGGTGATGAG GCGCAGAGCA AGCGTGGTAT CCTCACCCTG AAGTACCCCA
      901 TTGAGCACGG TATCGTGACC AACTGGGACG ATATGGAGAA GATCTGGCAC CACACCTTCT
      961 ACAATGAGCT GCGTGTGGCA CCCGAGGAGC ACCCCGTGCT GCTGACCGAG GCCCCGCTGA
     1021 ACCCCAAGGC CAACCGTGAG AAGATGACCC AGATCATGTT CGAGACCTTC AACACACCCG
     1081 CCATGTATGT GGCCATCCAG GCTGTGCTCT CGCTGTACGC CTCCGGTCGT ACCACCGGTA
     1141 TCGTTATGGA CTCCGGCGAT GGTGTCACCC ACACCGTGCC CATCTACGAG GGTTATGCCC
     1201 TTCCCCATGC CATCCTGCGT CTGGATCTGG CTGGTCGCGA TTTGACCGAC TACCTGATGA
     1261 AGATCCTGAC CGAGCGCGGT TACTCTTTCA CCACCACCGC TGAGCGTGAA ATCGTCCGTG
     1321 ACATCAAGGA GAAGCTGTGC TATGTTGCCC TCGACTTTGA GCAGGAGATG GCCACCGCTG
     1381 CCAGCAGCTC CTCGTTGGAG AAGTCCTACG AGCTGCCCGA CGGACAGGTG ATCACCATCG
     1441 GCAACGAGCG TTTCCGCTGC CCCGAGGCCC TGTTCCAGCC CTCGTTCTTG GGAATGGAGT
     1501 CCTGCGGCAT CCACGAGACC ACCTTCAACT CCATCATGAA GTGTGATGTG GATATCCGTA
     1561 AGGATCTGTA TGCCAACACC GTGCTGTCCG GTGGCACCAC CATGTACCCT GGCATCGCCG
     1621 ACCGTATGCA GAAGGAGATC ACCGCCCTGG CCCCGTCGAC CATGAAGATC AAGATCATTG
     1681 CCCCGCCAGA GCGCAAGTAC TCTGTCTGGA TCGGTGGCTC CATCCTGGCT TCGCTGTCCA
     1741 CCTTCCAGCA GATGTGGATC TCCAAGCAGG AGTACGACGA GTCCGGCCCC TCCATTGTGC
     1801 ACCGCAAGTG CTTCTAAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT
     1861 GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1921 GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAGGAGACA ATACCGGAAT CTAGAtaatc
     1981 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2041 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2101 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2161 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2221 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2281 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2341 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2401 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2461 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2521 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatCAA TTGTTGTTGT
     2581 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
     2641 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
     2701 TTAACGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA
     2761 TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT
     2821 AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG
     2881 TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC
     2941 CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA
     3001 AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG
     3061 GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG
     3121 TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC
     3181 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
     3241 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG
     3301 AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC
     3361 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA
     3421 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
     3481 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
     3541 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC
     3601 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG
     3661 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
     3721 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
     3781 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
     3841 CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG
     3901 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
     3961 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
     4021 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
     4081 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
     4141 GTCTTGTCGA TCAGGATGAT CTGGACGAGG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
     4201 TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
     4261 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC
     4321 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAGG
     4381 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT
     4441 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT
     4501 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC
     4561 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA
     4621 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG
     4681 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC
     4741 GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT
     4801 CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC
     4861 CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC
     4921 TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     4981 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     5041 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     5101 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     5161 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     5221 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     5281 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     5341 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     5401 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     5461 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     5521 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     5581 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     5641 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     5701 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     5761 TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
//