LOCUS       MBP-K365-GS2-Micro      7355 bp ds-DNA     circular     28-FEB-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    3099..3119
                     /label="TEV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            3129..4223
                     /label="Kinesin Heavy Chain (365)"
                     /ApEinfo_revcolor=#ff565b
                     /ApEinfo_fwdcolor=#ff565b
     primer_bind     complement(join(7355..7355,1..54))
                     /label="gene-specific primer: CAGGTTGGGCGTGCTTGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1938..3038
                     /label="MBP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1902..4577
                     /label="ORF"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     join(7324..7355,1..17)
                     /label="gene-specific primer: ATGTCCGCGGAACGAGAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4239..4577
                     /label="Micro"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1914..1931
                     /label="His-Tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
       61 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
      721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
      781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
      841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
      901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
      961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     1621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1681 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     1741 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagaaatcat
     1801 aaaaaattta tttgctttgt gagcggataa caattataat agattcaatt gtgagcggat
     1861 aacaatttca cacagaattc attaaagagg agaaattaac tatgagagga tcgcatcacc
     1921 atcaccatca cggatctaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
     1981 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
     2041 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
     2101 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
     2161 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
     2221 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
     2281 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
     2341 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
     2401 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
     2461 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
     2521 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
     2581 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
     2641 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
     2701 ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc
     2761 tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
     2821 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
     2881 cacgtattgc cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
     2941 agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc
     3001 agactgtcga tgaagccctg aaagacgcgc agactaattc gagctcgaac aacaacaaca
     3061 ataacaataa caacaacctc gggatcgagg gaacgaccga aaacctgtat tttcagggat
     3121 ccggtagtAT GTCCGCGGAA CGAGAGATTC CCGCCGAGGA CAGCATCAAA GTGGTCTGCC
     3181 GATTCCGACC GCTGAACGAC AGCGAAGAGA AGGCCGGCTC CAAGTTCGTG GTCAAGTTCC
     3241 CCAACAATGT GGAGGAGAAC TGCATATCCA TAGCGGGCAA GGTGTATTTG TTCGACAAGG
     3301 TCTTCAAACC GAATGCATCC CAGGAAAAGG TCTACAATGA GGCGGCCAAG TCCATTGTTA
     3361 CGGATGTCCT GGCCGGGTAC AATGGAACGA TATTCGCATA TGGTCAGACG TCCTCCGGAA
     3421 AAACGCATAC GATGGAGGGC GTGATCGGGG ACTCCGTAAA ACAGGGTATC ATACCACGTA
     3481 TCGTCAACGA CATTTTCAAT CACATCTACG CGATGGAGGT GAACCTGGAG TTTCACATCA
     3541 AGGTCTCCTA CTACGAGATC TACATGGACA AGATTCGAGA TCTGTTGGAC GTCTCCAAGG
     3601 TGAACCTTAG TGTGCACGAG GATAAGAACC GGGTGCCGTA CGTCAAGGGC GCTACGGAAC
     3661 GGTTCGTCTC GTCGCCGGAG GATGTTTTCG AGGTGATCGA GGAGGGCAAA TCCAATCGTC
     3721 ACATCGCTGT GACAAACATG AACGAGCATT CTTCGCGATC CCACTCAGTA TTCCTTATCA
     3781 ATGTGAAGCA GGAGAACCTG GAGAACCAGA AGAAACTATC CGGCAAACTC TACCTGGTGG
     3841 ATTTGGCCGG TTCCGAGAAG GTTTCCAAGA CTGGAGCGGA GGGAACCGTT CTTGATGAAG
     3901 CCAAGAACAT CAACAAGTCG CTGTCGGCCT TGGGCAACGT AATTTCTGCC CTGGCGGACG
     3961 GAAACAAAAC GCACATCCCC TACCGTGATT CCAAGCTAAC GCGCATCCTG CAGGAGTCGC
     4021 TGGGAGGCAA CGCACGCACA ACCATCGTCA TCTGCTGCTC TCCAGCCAGT TTCAACGAAT
     4081 CTGAAACGAA GTCAACGCTG GACTTCGGTC GTAGAGCCAA GACAGTGAAG AACGTGGTCT
     4141 GCGTTAACGA GGAGCTTACT GCCGAGGAAT GGAAGCGACG CTATGAAAAG GAGAAGGAAA
     4201 AGAACGCCCG ACTAAAGGGT AAGggtagtg gtagtggatc cagctccccg aaacgcccta
     4261 agctgctgcg tgaatattac gattggctgg ttgataacag ctttacccca tatctggtgg
     4321 tggatgccac atacctgggc gtgaacgtgc ccgtggagta tgtgaaagac ggtcagatcg
     4381 tgctgaatct gtctgcaagt gcgaccggca acctgcaact gacaaatgat tttatccagt
     4441 tcaacgccca gtttaagggc gtgtctcgtg aactgtatat cccgatgggt gccgctctgg
     4501 ccatttacgc tcgcgagaac ggcgatggtg tgatgttcga accagaagaa atctatgacg
     4561 agctgaatat tggttaaaag cttaattagc tgagcttgga ctcctgttga tagatccagt
     4621 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     4681 tattggtgag aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg
     4741 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     4801 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     4861 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     4921 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
     4981 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     5041 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     5101 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     5161 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     5221 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     5281 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     5341 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     5401 tttaaggcag ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat
     5461 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     5521 gtgaacgctc tcctgagtag gacaaatccg ccctctagat tacgtgcagt cgatgataag
     5581 ctgtcaaaca tgagaattgt gcctaatgag tgagctaact tacattaatt gcgttgcgct
     5641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5701 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     5761 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     5821 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     5881 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
     5941 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
     6001 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
     6061 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
     6121 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
     6181 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
     6241 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
     6301 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
     6361 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
     6421 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
     6481 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     6541 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     6601 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     6661 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     6721 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     6781 ttgcaccatt cgatggtgtc ggaatttcgg gcagcgttgg gtcctggcca cgggtgcgca
     6841 tgatctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6901 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6961 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7021 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7081 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     7141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7321 ccataggctc cgcccccctg acgagcatca caaaa
//