LOCUS       NupG_region_-_OptoCre-b 3772 bp ds-DNA     linear       30-SEP-2022
DEFINITION  .
COMMENT     Imported from genome: ASM584v2 (Escherichia coli str. K-12 substr.
            MG1655) Location: Chromosome 3103000-3109000
FEATURES             Location/Qualifiers
     gene            1..1257
                     /label="nupG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1211..1260
                     /label="nupG integrate Fwd Patange"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1291..1419)
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1429..2289)
                     /label="bla"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     RBS             complement(2290..2309)
                     /label="R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     complement(2310..2343)
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2346..2474)
                     /label="rrnBT1-T7TE terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_recomb     complement(2514..2547)
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(2548..2618)
                     /label="P"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2761..2794
                     /label="FRT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2810..2859
                     /label="nupG integrate rev Patange"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 ATGAATCTTA AGCTGCAGCT GAAAATCCTC TCTTTTCTGC AGTTCTGTCT GTGGGGAAGT
       61 TGGCTGACGA CCCTCGGCTC CTATATGTTT GTTACCCTGA AGTTTGACGG TGCTTCTATT
      121 GGCGCAGTTT ATAGCTCACT GGGTATCGCA GCGGTCTTTA TGCCTGCGCT GCTGGGGATT
      181 GTGGCCGACA AATGGTTAAG TGCGAAATGG GTATATGCCA TTTGCCACAC CATTGGCGCT
      241 ATCACGCTGT TCATGGCGGC ACAGGTCACG ACACCGGAAG CGATGTTCCT TGTGATATTG
      301 ATTAACTCGT TTGCTTATAT GCCAACGCTT GGGTTAATCA ACACCATCTC TTACTATCGC
      361 CTGCAAAATG CCGGGATGGA TATCGTTACT GACTTCCCGC CAATCCGTAT CTGGGGCACC
      421 ATCGGCTTTA TCATGGCAAT GTGGGTGGTG AGCCTGTCTG GCTTCGAATT AAGCCACATG
      481 CAGCTGTATA TTGGCGCAGC ACTTTCCGCC ATTCTGGTTC TGTTTACCCT GACTCTGCCG
      541 CATATTCCGG TTGCTAAACA GCAAGCGAAT CAGAGCTGGA CAACCCTGCT GGGCCTCGAT
      601 GCATTCGCGC TGTTTAAAAA CAAGCGTATG GCAATCTTCT TTATCTTCTC AATGCTGCTG
      661 GGCGCGGAAC TGCAGATTAC CAACATGTTC GGTAATACCT TCCTGCACAG CTTCGACAAA
      721 GATCCGATGT TTGCCAGCAG CTTTATTGTG CAGCATGCGT CAATCATCAT GTCGATTTCG
      781 CAGATCTCTG AAACCCTGTT CATTCTGACC ATCCCGTTCT TCTTAAGCCG CTACGGTATT
      841 AAGAACGTAA TGATGATCAG TATTGTGGCG TGGATCCTGC GTTTTGCGCT GTTTGCTTAC
      901 GGCGACCCGA CTCCGTTCGG TACTGTACTG CTGGTACTGT CGATGATCGT TTACGGTTGC
      961 GCATTCGACT TCTTCAACAT CTCTGGTTCG GTGTTTGTCG AAAAAGAAGT TAGCCCGGCA
     1021 ATTCGCGCCA GTGCACAAGG GATGTTCCTG ATGATGACTA ACGGCTTCGG CTGTATCCTC
     1081 GGCGGCATCG TGAGCGGTAA AGTTGTTGAG ATGTACACCC AAAACGGCAT TACCGACTGG
     1141 CAGACCGTAT GGTTGATTTT CGCTGGTTAC TCCGTGGTTC TGGCCTTCGC GTTCATGGCG
     1201 ATGTTCAAAT ATAAACACGT TCGTGTCCCG ACAGGCACAC AGACGGTTAG CCACTAATTA
     1261 gtgagcgagg aagcggaata tatccctagg tataaacgca gaaaggccca cccgaaggtg
     1321 agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     1381 agtctttcga ctgagccttt cgttttattt gatgcctgga gatccctatt aCCaatgctt
     1441 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1501 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1561 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1621 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1681 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1741 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1801 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1861 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1921 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1981 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2041 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2101 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2161 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2221 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2281 aatactcatA TGTATATCTC CTTCTTAAAa taacttcgta taatgtatgc tatacgaagt
     2341 tatggtataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
     2401 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     2461 tatttgatgc ctggagatcc ttactcgagt ttggatcctt atttgatgcc tggataactt
     2521 cgtataatgt atgctatacg aagttatTTC GCCGTGTCCC TCTCGATGGC TGTAAGATTC
     2581 CTATAGGTTA GACTTTAATG CAATACTCTT TTTGATAAct caaagggcct cgtgataata
     2641 ggaacttcgg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     2701 tcgccgacat caccgatggg gaagatcctg tcaaacatga gaattaattc cggggatctt
     2761 gaagttccta ttctctagaa agtataggaa cttcgaagca gctccagccC GCAAAGAAAA
     2821 ACGGGTCGCC AGAAGGTGAC CCGTTTTTTT TATTCTTACT TCAACACATA ACCGTACAAC
     2881 CGTTTCACGC CATCCGCATC GGTTTCGCTA TAAACACCTT GCAGCTCCGG CGAAAATCCC
     2941 GGCAACAAAT TCACCCCTTC TTCCAGTGCA AGGAAATAAC GTTGAACCGC CCCACCCCAG
     3001 ACTTCCCCGG GTACCACGCA AAGCACGCCA GGTGGATAAG GCAACGCCCC TTCTGCCGCA
     3061 ATTCGCCCTT CGGCATCACG AATCCGCACC AACTCCACGT CACCGCGAAT ATAAGCGCTA
     3121 TGCGCATCCT GGGGGTTCAT CACCACTGAC GGGAAACTCT GCTGGCGGAA CATCGCTTTT
     3181 TGTAGGTCTT TGACGTCGAA ACTGACATAC AGATCGTGCA TCTCCTGACA CAACTGGCGC
     3241 AGGGTGTAGT CGCGATAGCG CACCGGATAC TTGTTATAAA CGCTCGGCAA CACCTCAACC
     3301 AGCGGCGAGT CATCCTCAAT ATGCTGTTCA AATTGCGCCA GCATCGCCAC CAGTTGTGCC
     3361 AGCTTCTCGT GGCTTTCCGC CGGAGTTAAT AAAAACAGAA TGGAGTTGAG ATCGCACTTC
     3421 TCCGGCACAA TGCCGTTCTC ACGCAGATAG TGCGCCAGAA TCGTCGCCGG AACGCCAAAG
     3481 TCGCTATATT CGCCGGTTTC GGCATCGATA CCTGGTGTAG TGAGTAACAG CTTGCACGGA
     3541 TCAACAAAAT ACTGATCCGC GGCATATCCT TCAAAGCCGT GCCACTTCGC CCCCGGCTCA
     3601 AAACTGAAAA AACGGCGGTC GCTGGCTAAC ACTGATGTCG GATAATCCTG CCACAATTTG
     3661 CCATCAACAA CGGGCGGGAT AAACGGGCGG AACAGCTTAC AGCGCGCAAG AATAGCCTTG
     3721 CGCGCTTCAA TCCCTATCTC AACACACTCA GCCCACAGCC GACGCCCACT CT
//