LOCUS       NupG_region_-_OptoCre-k 3723 bp ds-DNA     linear       13-SEP-2022
DEFINITION  .
COMMENT     Imported from genome: ASM584v2 (Escherichia coli str. K-12 substr.
            MG1655) Location: Chromosome 3103000-3109000
FEATURES             Location/Qualifiers
     gene            1..1257
                     /label="nupG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1211..1260
                     /label="nupG integrate Fwd Patange"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1291..1419)
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1432..2226)
                     /label="knt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(1432..2226)
                     /label="Translation 3105225-3104431"
     RBS             complement(2227..2246)
                     /label="R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     complement(2247..2280)
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2283..2411)
                     /label="rrnBT1-T7TE terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_recomb     complement(2451..2484)
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2485..2555)
                     /label="P*"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2711..2745
                     /label="FRT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2761..2810
                     /label="nupG integrate rev Patange"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 ATGAATCTTA AGCTGCAGCT GAAAATCCTC TCTTTTCTGC AGTTCTGTCT GTGGGGAAGT
       61 TGGCTGACGA CCCTCGGCTC CTATATGTTT GTTACCCTGA AGTTTGACGG TGCTTCTATT
      121 GGCGCAGTTT ATAGCTCACT GGGTATCGCA GCGGTCTTTA TGCCTGCGCT GCTGGGGATT
      181 GTGGCCGACA AATGGTTAAG TGCGAAATGG GTATATGCCA TTTGCCACAC CATTGGCGCT
      241 ATCACGCTGT TCATGGCGGC ACAGGTCACG ACACCGGAAG CGATGTTCCT TGTGATATTG
      301 ATTAACTCGT TTGCTTATAT GCCAACGCTT GGGTTAATCA ACACCATCTC TTACTATCGC
      361 CTGCAAAATG CCGGGATGGA TATCGTTACT GACTTCCCGC CAATCCGTAT CTGGGGCACC
      421 ATCGGCTTTA TCATGGCAAT GTGGGTGGTG AGCCTGTCTG GCTTCGAATT AAGCCACATG
      481 CAGCTGTATA TTGGCGCAGC ACTTTCCGCC ATTCTGGTTC TGTTTACCCT GACTCTGCCG
      541 CATATTCCGG TTGCTAAACA GCAAGCGAAT CAGAGCTGGA CAACCCTGCT GGGCCTCGAT
      601 GCATTCGCGC TGTTTAAAAA CAAGCGTATG GCAATCTTCT TTATCTTCTC AATGCTGCTG
      661 GGCGCGGAAC TGCAGATTAC CAACATGTTC GGTAATACCT TCCTGCACAG CTTCGACAAA
      721 GATCCGATGT TTGCCAGCAG CTTTATTGTG CAGCATGCGT CAATCATCAT GTCGATTTCG
      781 CAGATCTCTG AAACCCTGTT CATTCTGACC ATCCCGTTCT TCTTAAGCCG CTACGGTATT
      841 AAGAACGTAA TGATGATCAG TATTGTGGCG TGGATCCTGC GTTTTGCGCT GTTTGCTTAC
      901 GGCGACCCGA CTCCGTTCGG TACTGTACTG CTGGTACTGT CGATGATCGT TTACGGTTGC
      961 GCATTCGACT TCTTCAACAT CTCTGGTTCG GTGTTTGTCG AAAAAGAAGT TAGCCCGGCA
     1021 ATTCGCGCCA GTGCACAAGG GATGTTCCTG ATGATGACTA ACGGCTTCGG CTGTATCCTC
     1081 GGCGGCATCG TGAGCGGTAA AGTTGTTGAG ATGTACACCC AAAACGGCAT TACCGACTGG
     1141 CAGACCGTAT GGTTGATTTT CGCTGGTTAC TCCGTGGTTC TGGCCTTCGC GTTCATGGCG
     1201 ATGTTCAAAT ATAAACACGT TCGTGTCCCG ACAGGCACAC AGACGGTTAG CCACTAATTA
     1261 gtgagcgagg aagcggaata tatccctagg tataaacgca gaaaggccca cccgaaggtg
     1321 agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     1381 agtctttcga ctgagccttt cgttttattt gatgcctgga gatccctatt atcagaagaa
     1441 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     1501 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     1561 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     1621 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     1681 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     1741 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     1801 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     1861 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     1921 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     1981 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     2041 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     2101 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     2161 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     2221 aatcatATGT ATATCTCCTT CTTAAAataa cttcgtataa tgtatgctat acgaagttat
     2281 ggtataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
     2341 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     2401 ttgatgcctg gagatcctta ctcgagtttg gatccttatt tgatgcctgg ataacttcgt
     2461 ataatgtatg ctatacgaag ttatTTCGCC GTGTCCCTCT CGATGGCTGT AAGAATCCTA
     2521 TAGGTTAGAC TTTAAGACAA TACTCTTTTT GATAActcaa agggcctcgt gataatagga
     2581 acttcggcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     2641 ccgacatcac cgatggggaa gatcctgtca aacatgagaa ttaattccgg ggatccgtcg
     2701 acctgcagtt cgaagttcct attctctaga aagtatagga acttcgaagc agctccagcc
     2761 CGCAAAGAAA AACGGGTCGC CAGAAGGTGA CCCGTTTTTT TTATTCTTAC TTCAACACAT
     2821 AACCGTACAA CCGTTTCACG CCATCCGCAT CGGTTTCGCT ATAAACACCT TGCAGCTCCG
     2881 GCGAAAATCC CGGCAACAAA TTCACCCCTT CTTCCAGTGC AAGGAAATAA CGTTGAACCG
     2941 CCCCACCCCA GACTTCCCCG GGTACCACGC AAAGCACGCC AGGTGGATAA GGCAACGCCC
     3001 CTTCTGCCGC AATTCGCCCT TCGGCATCAC GAATCCGCAC CAACTCCACG TCACCGCGAA
     3061 TATAAGCGCT ATGCGCATCC TGGGGGTTCA TCACCACTGA CGGGAAACTC TGCTGGCGGA
     3121 ACATCGCTTT TTGTAGGTCT TTGACGTCGA AACTGACATA CAGATCGTGC ATCTCCTGAC
     3181 ACAACTGGCG CAGGGTGTAG TCGCGATAGC GCACCGGATA CTTGTTATAA ACGCTCGGCA
     3241 ACACCTCAAC CAGCGGCGAG TCATCCTCAA TATGCTGTTC AAATTGCGCC AGCATCGCCA
     3301 CCAGTTGTGC CAGCTTCTCG TGGCTTTCCG CCGGAGTTAA TAAAAACAGA ATGGAGTTGA
     3361 GATCGCACTT CTCCGGCACA ATGCCGTTCT CACGCAGATA GTGCGCCAGA ATCGTCGCCG
     3421 GAACGCCAAA GTCGCTATAT TCGCCGGTTT CGGCATCGAT ACCTGGTGTA GTGAGTAACA
     3481 GCTTGCACGG ATCAACAAAA TACTGATCCG CGGCATATCC TTCAAAGCCG TGCCACTTCG
     3541 CCCCCGGCTC AAAACTGAAA AAACGGCGGT CGCTGGCTAA CACTGATGTC GGATAATCCT
     3601 GCCACAATTT GCCATCAACA ACGGGCGGGA TAAACGGGCG GAACAGCTTA CAGCGCGCAA
     3661 GAATAGCCTT GCGCGCTTCA ATCCCTATCT CAACACACTC AGCCCACAGC CGACGCCCAC
     3721 TCT
//