LOCUS       Exported               11667 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
     LTR             217..397
                     /label=5' LTR (truncated)
     misc_feature    444..569
                     /label=HIV-1 Psi
     misc_feature    553..917
                     /label=gag
     misc_feature    1062..1295
                     /label=RRE
     misc_feature    1822..1939
                     /label=cPPT/CTS
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
     regulatory      2427..2435
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)
     CDS             2436..5390
                     /label=DpCasX (DpCas12e)
     misc_feature    4446..4448
                     /label=D672
     misc_feature    4737..4739
                     /label=E769
     misc_feature    5235..5237
                     /label=D935
     CDS             5391..5438
                     /label=nucleoplasmin NLS
     CDS             5439..5462
                     /label=FLAG
     CDS             5472..5528
                     /label=P2A
     CDS             5529..6125
                     /label=PuroR
     misc_feature    6141..6729
                     /label=WPRE
     CDS             complement(6612..6623)
                     /label=Factor Xa site
     LTR             6801..7034
                     /label=3' LTR (Delta-U3)
     polyA_signal    7066..7290
                     /label=bGH poly(A) signal
     rep_origin      7336..7764
                     /label=f1 ori
     promoter        7778..8107
                     /label=SV40 promoter
     rep_origin      7958..8093
                     /label=SV40 ori
     promoter        8155..8202
                     /label=EM7 promoter
     CDS             8221..8595
                     /label=BleoR
     polyA_signal    8725..8846
                     /label=SV40 poly(A) signal
     protein_bind    8919..8935
                     /label=lac operator
     promoter        complement(8943..8973)
                     /label=lac promoter
     protein_bind    8988..9009
                     /label=CAP binding site
     rep_origin      complement(9297..9885)
                     /direction=LEFT
                     /label=ori
     rep_origin      9484..9709
                     /label=ColE1
     CDS             complement(10056..10916)
                     /label=AmpR
     promoter        complement(10917..11021)
                     /label=AmpR promoter
     enhancer        11287..11666
                     /label=CMV enhancer
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatggaaaa gagaatcaac aagatcagga
     2461 agaagctgag cgccgacaac gccaccaagc ctgtgtctag gagtggcccc atgaaaaccc
     2521 tgctcgtcag agtgatgacc gacgacctga agaagagact ggaaaagagg aggaagaaac
     2581 ccgaagtgat gccccaagtg atcagcaaca acgccgccaa caacctgaga atgctgctgg
     2641 acgactacac caagatgaag gaagccatcc tgcaggtcta ctggcaagag ttcaaggacg
     2701 accacgtggg cctgatgtgc aagtttgccc agcctgccag caagaagatc gaccagaaca
     2761 agctgaagcc cgagatggac gagaagggca atctgaccac agccggcttt gcctgctctc
     2821 agtgtggcca gcctctgttc gtgtacaagc tggaacaggt gtccgagaaa ggcaaggcct
     2881 acaccaacta cttcggcaga tgtaacgtgg ccgagcacga gaagctgatt ctgctggccc
     2941 agctgaaacc tgagaaggac tctgatgagg ccgtgaccta cagcctgggc aagtttggac
     3001 agagagccct ggacttctac agcatccacg tgaccaaaga aagcacacac cccgtgaagc
     3061 ccctggctca gatcgccggc aatagatacg cctctggacc tgtgggcaaa gccctgtccg
     3121 atgcctgcat gggaacaatc gccagcttcc tgagcaagta ccaggacatc atcatcgagc
     3181 accagaaggt ggtcaagggc aaccagaaga gactggaaag cctgagggag ctggccggca
     3241 aagagaacct ggaatacccc agcgtgaccc tgcctcctca gcctcacaca aaagaaggcg
     3301 tggacgccta caacgaagtg atcgccagag tgagaatgtg ggtcaacctg aacctgtggc
     3361 agaagctgaa actgtccagg gacgacgcca agcctctgct gagactgaag ggcttcccta
     3421 gcttccccgt ggtggaaaga agggagaatg aggtggactg gtggaacacc atcaacgaag
     3481 tcaagaaact gatcgatgcc aagagagaca tgggcagagt gttttggagc ggagtgaccg
     3541 ccgagaagag aaacaccatc ctggaaggct acaactacct gcctaacgag aacgaccaca
     3601 agaagagaga gggcagcctg gaaaacccta agaagcccgc caagagacag ttcggcgacc
     3661 tgctgctgta cctggagaag aagtatgccg gcgactgggg caaagtgttc gatgaggcct
     3721 gggagagaat cgacaagaag attgccggcc tgaccagcca catcgagaga gaggaagcca
     3781 gaaacgccga ggacgcccag tctaaagccg tgctgacaga ttggctgaga gccaaggcca
     3841 gcttcgtgct ggaaaggctg aaagaaatgg atgagaaaga gttctacgcc tgcgagatcc
     3901 agctgcagaa gtggtacggg gacctgagag gcaatccctt tgccgtggaa gccgagaaca
     3961 gagtggtgga catcagcggc tttagcatcg gctctgacgg ccactccatc cagtacagga
     4021 acctgctggc ctggaagtac ctggaaaatg gcaagagaga gttctacctg ctgatgaact
     4081 acggcaagaa gggcagaatc agattcaccg acggcaccga catcaagaaa agcggcaagt
     4141 ggcagggcct gctgtatggc ggcggaaagg ccaaagtgat cgacctgacc ttcgatcccg
     4201 acgacgagca gctgatcatt ctgcctctgg ccttcggcac cagacagggc agagagttca
     4261 tctggaacga tctgctgtcc ctggaaaccg gcctgatcaa gctggccaac ggcagggtga
     4321 tcgagaaaac catctacaac aagaaaatcg gcagagatga gcccgcactg tttgtggccc
     4381 tgacctttga gaggagagaa gtggtcgatc ccagcaacat caagcccgtg aacctgatcg
     4441 gagtggacag aggcgagaat atccctgccg tgattgccct gacagaccct gagggatgtc
     4501 ccctgcctga gtttaaggat agctctggcg gccctaccga catcctgaga atcggcgagg
     4561 gctacaaaga gaagcagaga gccatccagg ccgccaaaga ggtggaacag agaagagccg
     4621 gcggatacag cagaaagttc gccagcaagt ccagaaacct ggccgatgac atggtcagga
     4681 acagcgccag ggacctgttc taccacgccg tgacacatga tgccgtgctg gtgttcgaga
     4741 acctgtcaag aggcttcggc agacagggaa aaagaacctt catgaccgag agacagtaca
     4801 cgaagatgga agattggctc accgccaaac tggcctacga gggcctgaca agcaagacct
     4861 acctgtccaa gacactggcc cagtacacct ccaagacctg ctccaattgc ggcttcacca
     4921 tcaccaccgc cgactacgat ggaatgctcg tgagactgaa gaaaacctcc gacggctggg
     4981 ccaccacact gaacaacaaa gagctgaaag ccgagggcca gatcacctac tacaacagat
     5041 acaagaggca gaccgtcgag aaagagctgt ccgccgaact cgacagactg agcgaggaat
     5101 ccggcaacaa cgacatcagc aagtggacca agggacgcag agatgaggcc ctgttcctgc
     5161 tgaagaagag gttcagccac agacctgtgc aagagcagtt tgtgtgcctg gattgcggcc
     5221 acgaagtgca cgcagatgaa caggccgctc tgaatatcgc cagaagctgg ctgttcctga
     5281 acagcaacag caccgagttc aagagctaca agagcggcaa gcagcctttc gttggagcct
     5341 ggcaggcctt ctacaagaga aggctcaaag aagtgtggaa gcccaacgcc aagcgacctg
     5401 ccgccacaaa gaaggctgga caggctaaga agaagaaaga ttacaaagac gatgacgata
     5461 agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
     5521 ctggaccgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     5581 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     5641 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     5701 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     5761 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5821 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5881 aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc
     5941 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6001 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6061 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6121 cctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6541 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6721 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6781 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6841 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7021 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     7081 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7141 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7201 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7261 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7321 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7381 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7681 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7741 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7861 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7921 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7981 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8041 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8101 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     8161 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     8221 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     8281 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     8341 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     8401 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     8461 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     8521 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     8581 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8701 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8881 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11041 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    11101 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    11161 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    11221 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    11281 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    11341 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    11401 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    11461 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    11521 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
    11581 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
    11641 acgtattagt catcgctatt accatgg
//
