LOCUS       Exported               11007 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
     LTR             217..397
                     /label=5' LTR (truncated)
     misc_feature    444..569
                     /label=HIV-1 Psi
     misc_feature    553..917
                     /label=gag
     misc_feature    1062..1295
                     /label=RRE
     misc_feature    1822..1939
                     /label=cPPT/CTS
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
     regulatory      2427..2435
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)
     misc_feature    2436..4730
                     /label=Cas-Phi-3 (CasF-3)
     CDS             4731..4778
                     /label=nucleoplasmin NLS
     CDS             4779..4802
                     /label=FLAG
     CDS             4812..4868
                     /label=P2A
     CDS             4869..5465
                     /label=PuroR
     misc_feature    5481..6069
                     /label=WPRE
     CDS             complement(5952..5963)
                     /label=Factor Xa site
     LTR             6141..6374
                     /label=3' LTR (Delta-U3)
     polyA_signal    6406..6630
                     /label=bGH poly(A) signal
     rep_origin      6676..7104
                     /label=f1 ori
     promoter        7118..7447
                     /label=SV40 promoter
     rep_origin      7298..7433
                     /label=SV40 ori
     promoter        7495..7542
                     /label=EM7 promoter
     CDS             7561..7935
                     /label=BleoR
     polyA_signal    8065..8186
                     /label=SV40 poly(A) signal
     protein_bind    8259..8275
                     /label=lac operator
     promoter        complement(8283..8313)
                     /label=lac promoter
     protein_bind    8328..8349
                     /label=CAP binding site
     rep_origin      complement(8637..9225)
                     /direction=LEFT
                     /label=ori
     rep_origin      8824..9049
                     /label=ColE1
     CDS             complement(9396..10256)
                     /label=AmpR
     promoter        complement(10257..10361)
                     /label=AmpR promoter
     enhancer        10627..11006
                     /label=CMV enhancer
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatggaaaa agaaataact gagctcacca
     2461 agattaggcg cgagtttccg aataaaaagt tcagcagcac tgatatgaag aaggcaggta
     2521 agttgttgaa ggcagaaggt cctgatgctg ttagagactt cctgaactcc tgccaggaga
     2581 ttatcgggga ttttaagccg cctgtaaaga caaacatagt cagcatatca cgaccctttg
     2641 aggagtggcc tgttagtatg gtggggcgcg ccatccagga atattacttt agtttgacaa
     2701 aagaggaatt ggagtccgtc catcccggaa cttccagcga ggatcacaag tccttcttta
     2761 acataactgg cctgagcaat tacaattata cgtcagtcca aggcttgaat ctcatcttca
     2821 aaaatgcgaa ggccatatac gacgggactc tggttaaagc aaacaataaa aataagaagt
     2881 tggaaaaaaa gttcaatgag attaaccaca agcgaagcct tgaggggctt cctataatta
     2941 cgccggattt cgaggaaccc tttgatgaga atggccatct gaataatccg ccaggtatta
     3001 atcgaaatat ttacggctac caaggatgtg ccgctaaagt attcgttcct tccaagcata
     3061 aaatggtatc cctccctaaa gaatacgaag ggtacaaccg ggatccgaac ctgtccttgg
     3121 cgggcttccg aaatcggctc gagataccgg agggggagcc cggtcacgtg ccatggtttc
     3181 agcgcatgga tatcccggaa ggccagatcg ggcacgtaaa taagattcaa cgattcaatt
     3241 tcgttcatgg caagaattca ggaaaagtca aattcagcga taagacagga cgggtaaaac
     3301 gctaccatca ttccaagtat aaagatgcca ctaagcctta caaatttctt gaagaatcca
     3361 agaaagtcag tgctctggac tccatccttg ccattatcac aatcggtgat gactgggtag
     3421 tgtttgacat tcgcggtctg tatagaaatg ttttttatcg cgaactggca cagaagggcc
     3481 tgacagcagt gcagctgctg gatctgttta cgggggatcc ggtgattgac ccgaagaagg
     3541 gcgttgtgac attcagctat aaggaaggcg tggttccagt attttcacag aagatcgttc
     3601 caaggttcaa gagtcgagac acgctcgaga aattgaccag tcaaggacct gtggcgctgc
     3661 tctcagtcga cctcggccaa aatgaaccag tggcggcaag ggtttgtagc ttgaagaaca
     3721 taaatgataa gatcacattg gataattctt gcagaatctc cttcctggat gactacaaaa
     3781 aacaaatcaa agactacaga gattccctgg acgaacttga aatcaagata cgactggaag
     3841 caatcaattc tctggaaact aaccaacaag tagaaattcg cgacctggat gtattcagtg
     3901 ctgatcgggc aaaggcaaac actgtagata tgttcgacat cgacccaaat ttgatatcct
     3961 gggattcaat gagcgacgcg agggtgagca cgcaaataag cgatctttat ctgaagaatg
     4021 ggggtgacga atctcgagta tatttcgaaa ttaacaacaa acggataaag cgatctgatt
     4081 ataacattag tcagctggtg aggccaaagc tttccgacag cactcggaag aatctgaacg
     4141 attctatatg gaagttgaaa agaactagtg aagaatattt gaaattgtcc aaacgaaagt
     4201 tggaactgag cagagctgtt gtgaactaca ctatccgcca gagcaagctc ctctccggaa
     4261 ttaacgacat tgttataata cttgaggacc tggatgtaaa aaaaaaattc aatggcaggg
     4321 gcattcgaga tatcggatgg gacaacttct tcagctccag gaaagagaac aggtggttca
     4381 ttccggcatt ccataaggct ttctcagagc tttcaagcaa ccggggcctc tgtgtcatcg
     4441 aagtcaaccc ggcatggaca tctgccacct gtcccgactg cgggttctgt agtaaagaga
     4501 acagagatgg cattaatttt acctgtcgca agtgcggtgt ctcttaccac gcggacatag
     4561 atgttgccac tcttaatata gcccgggtgg ccgttctcgg caagcctatg tccggacccg
     4621 ccgaccgcga gagactgggc gatactaaga aaccccgggt agcaaggagc cgaaagacta
     4681 tgaaacggaa agatattagc aatagcaccg ttgaggctat ggttacagcc aagcgacctg
     4741 ccgccacaaa gaaggctgga caggctaaga agaagaaaga ttacaaagac gatgacgata
     4801 agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
     4861 ctggaccgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4921 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4981 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     5041 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     5101 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5161 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5221 aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc
     5281 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5341 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5401 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5461 cctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6001 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6061 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6121 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6181 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6361 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6421 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6481 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6541 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6601 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6661 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6781 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6841 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6901 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6961 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7021 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7081 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7201 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7381 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7441 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7501 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7561 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     7621 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     7681 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7741 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7801 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     7861 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     7921 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7981 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8041 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8101 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8161 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9061 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10381 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    10441 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    10501 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    10561 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    10621 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    10681 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    10741 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    10801 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    10861 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
    10921 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
    10981 acgtattagt catcgctatt accatgg
//
