LOCUS       Exported               10980 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
     LTR             217..397
                     /label=5' LTR (truncated)
     misc_feature    444..569
                     /label=HIV-1 Psi
     misc_feature    553..917
                     /label=gag
     misc_feature    1062..1295
                     /label=RRE
     misc_feature    1822..1939
                     /label=cPPT/CTS
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
     regulatory      2427..2435
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)
     misc_feature    2436..4703
                     /label=Cas-Phi-2 (CasF-2)
     CDS             4704..4751
                     /label=nucleoplasmin NLS
     CDS             4752..4775
                     /label=FLAG
     CDS             4785..4841
                     /label=P2A
     CDS             4842..5438
                     /label=PuroR
     misc_feature    5454..6042
                     /label=WPRE
     CDS             complement(5925..5936)
                     /label=Factor Xa site
     LTR             6114..6347
                     /label=3' LTR (Delta-U3)
     polyA_signal    6379..6603
                     /label=bGH poly(A) signal
     rep_origin      6649..7077
                     /label=f1 ori
     promoter        7091..7420
                     /label=SV40 promoter
     rep_origin      7271..7406
                     /label=SV40 ori
     promoter        7468..7515
                     /label=EM7 promoter
     CDS             7534..7908
                     /label=BleoR
     polyA_signal    8038..8159
                     /label=SV40 poly(A) signal
     protein_bind    8232..8248
                     /label=lac operator
     promoter        complement(8256..8286)
                     /label=lac promoter
     protein_bind    8301..8322
                     /label=CAP binding site
     rep_origin      complement(8610..9198)
                     /direction=LEFT
                     /label=ori
     rep_origin      8797..9022
                     /label=ColE1
     CDS             complement(9369..10229)
                     /label=AmpR
     promoter        complement(10230..10334)
                     /label=AmpR promoter
     enhancer        10600..10979
                     /label=CMV enhancer
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatgccaaa gccagccgtg gagtctgagt
     2461 tttctaaggt actcaagaag cactttccgg gcgagcgatt taggtctagc tacatgaagc
     2521 ggggtggtaa aatcttggca gcccagggtg aagaagcggt cgtcgcgtat ctgcaaggca
     2581 agtccgagga ggaacccccg aattttcagc cgccggcgaa atgtcatgtt gttacgaaat
     2641 cacgagattt cgccgagtgg ccaattatga aggcctccga agcaatccaa aggtatatct
     2701 atgcgctctc tacgacggaa cgggcagctt gcaagcctgg caaatcttca gagtcccacg
     2761 cggcctggtt cgcggcaact ggcgtgtcaa accacggtta tagccatgtt caaggcctca
     2821 atcttatctt cgaccacacg ctgggaagat acgatggtgt tctgaaaaag gtgcagctga
     2881 gaaatgagaa agcccgcgcc cggctggaaa gtatcaacgc ctctcgagcc gacgaaggac
     2941 ttccagaaat aaaggcagag gaggaagagg tcgctacaaa tgaaaccgga caccttttgc
     3001 agcctccggg gatcaaccca agtttctacg tttaccagac tatttctccg caggcttaca
     3061 ggccgcgaga tgagattgta ctgccgcccg agtatgccgg ctacgtccga gatccgaacg
     3121 cccctatccc ccttggcgtg gttcggaatc ggtgcgatat tcagaaggga tgccctggat
     3181 acatccccga atggcaaaga gaggcaggta ctgcaatttc ccctaagacg ggtaaagccg
     3241 tcaccgttcc cggcctcagt ccaaaaaaaa ataaacgaat gcgacgatac tggaggtccg
     3301 agaaagagaa ggcccaagat gcactgctcg ttactgtgag aatcggcact gactgggtcg
     3361 taatcgacgt tcgaggtttg ctgcggaatg cgcggtggcg caccattgcg cccaaggata
     3421 tatccttgaa tgccctcttg gatctcttta caggcgaccc ggtcatagat gttcggagaa
     3481 acattgtgac tttcacctac actctggacg cttgcggtac atatgctcgc aaatggactc
     3541 tcaaagggaa acagactaag gcaaccctcg ataagttgac cgcaacccag accgtggccc
     3601 tggtagcaat agaccttgga caaaccaatc ccataagtgc gggtatcagt agggtcacgc
     3661 aagaaaacgg ggcacttcaa tgtgaacctc tggatcggtt cactctccct gatgatctgc
     3721 tcaaggatat ctccgcgtac cgaatcgctt gggatcgcaa cgaggaggaa ctgagggcta
     3781 ggtccgtcga agcgctccca gaagctcaac aagctgaagt gagggctctg gacggcgttt
     3841 ctaaagaaac cgccaggacc cagctctgcg cggacttcgg ccttgatccc aaacggctgc
     3901 cttgggataa aatgagcagc aacaccactt tcatcagtga agcgttgctt agtaattctg
     3961 tgtctagaga tcaggttttt tttactcctg cgcctaaaaa gggagcaaag aaaaaagccc
     4021 ccgttgaagt tatgcggaag gataggacct gggcgagggc ctataaacca cggctcagtg
     4081 tggaagccca aaagctgaaa aatgaggcct tgtgggctct caagcgcact tctccagaat
     4141 acctcaagct gagtcggaga aaagaggagc tttgtaggcg aagtattaac tacgtcattg
     4201 aaaaaacaag acggaggaca caatgtcaga tcgtgatacc tgtcatagag gacttgaatg
     4261 tgcgattctt tcacggttca gggaagcgcc tgcctggctg ggataatttt ttcactgcga
     4321 agaaggagaa caggtggttt atacagggcc tccacaaagc attcagcgac ttgcgaactc
     4381 atcgctcctt ctacgtattc gaagtccgcc cggagcggac ttcaataacg tgcccaaaat
     4441 gcgggcactg cgaggttggg aaccgggatg gggaggcttt tcagtgcctt agttgcggca
     4501 aaacgtgcaa tgccgacctt gacgtggcta cccataatct gactcaagtc gcccttacag
     4561 gaaaaacaat gccgaaacgc gaggaaccta gagatgccca gggcacagct ccagcccgaa
     4621 aaacaaagaa ggcgtcaaag agcaaggctc cgccagccga acgagaggac caaactccag
     4681 cacaggaacc gtcccagact tccaagcgac ctgccgccac aaagaaggct ggacaggcta
     4741 agaagaagaa agattacaaa gacgatgacg ataagggatc cggcgcaaca aacttctctc
     4801 tgctgaaaca agccggagat gtcgaagaga atcctggacc gaccgagtac aagcccacgg
     4861 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4921 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4981 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5041 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5101 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5161 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5221 tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     5281 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5341 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5401 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac
     5461 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5521 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5581 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5641 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5701 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5761 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5821 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     5881 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5941 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6001 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     6061 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6121 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6181 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6241 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6301 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     6361 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6421 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6481 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6541 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6601 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7021 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7081 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7441 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     7501 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     7561 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     7621 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     7681 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     7741 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     7801 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     7861 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     7921 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7981 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8161 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10261 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10321 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10381 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    10441 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
    10501 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
    10561 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10621 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10681 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10741 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10801 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    10861 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10921 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
//
