LOCUS       Exported               12948 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12948
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_RNA        2060..2135
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             2433..6353
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      6073..6082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6354..6401
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6402..6425
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6459..6653
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB)
                     transcriptionalrepression domain from the human zinc finger
                     protein ZNF10 (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             6720..6740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6753..6809
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6810..7406
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7422..8010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(7893..7904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8082..8315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8347..8571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8617..9045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9059..9388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9239..9374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9436..9483
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9502..9876
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10006..10127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10176..10192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10200..10216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10224..10254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10269..10290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10578..11166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11337..12197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12198..12302)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        12568..12947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatgacaca gttcgagggc tttaccaacc
     2461 tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc
     2521 acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc
     2581 tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc
     2641 agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg
     2701 agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact
     2761 tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca
     2821 agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga
     2881 ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct
     2941 ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc
     3001 cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac
     3061 gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg
     3121 gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc
     3181 tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag
     3241 gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg
     3301 agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc
     3361 tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga
     3421 tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg
     3481 aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga
     3541 agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt
     3601 atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc
     3661 agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg
     3721 agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc
     3781 tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc
     3841 agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca
     3901 acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt
     3961 ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga
     4021 agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg aataaggaga
     4081 agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa
     4141 agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct
     4201 ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca
     4261 cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca
     4321 acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga
     4381 aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca
     4441 gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga
     4501 caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt
     4561 actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg
     4621 agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact
     4681 ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt
     4741 ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc
     4801 gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga
     4861 agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg
     4921 tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga
     4981 tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct
     5041 ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc
     5101 agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgcccggg
     5161 gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc
     5221 ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg
     5281 agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg
     5341 gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg
     5401 tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg
     5461 ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg
     5521 actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca
     5581 cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata
     5641 catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga
     5701 atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa
     5761 ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc
     5821 ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca
     5881 agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca
     5941 ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg
     6001 gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc
     6061 acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg
     6121 ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg
     6181 actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca
     6241 tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc
     6301 agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaagcgac
     6361 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg
     6421 ataagggatc cgatgctaag tcactgactg cctggtcccg gacactggtg accttcaagg
     6481 atgtgtttgt ggacttcacc agggaggagt ggaagctgct ggacactgct cagcagatcc
     6541 tgtacagaaa tgtgatgctg gagaactata agaacctggt ttccttgggt tatcagctta
     6601 ctaagccaga tgtgatcctc cggttggaga agggagaaga gccctggctg gtggagagag
     6661 aaattcacca agagacccat cctgattcag agactgcatt tgaaatcaaa tcatcagttc
     6721 cgaaaaagaa acgcaaagtt gctagctccg gcgcaacaaa cttctctctg ctgaaacaag
     6781 ccggagatgt cgaagagaat cctggaccga ccgagtacaa gcccacggtg cgcctcgcca
     6841 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6901 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6961 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7021 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7081 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7141 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc
     7201 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7261 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7321 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7381 gcatgacccg caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca
     7441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7801 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7981 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     8041 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8101 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8281 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     8341 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8401 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8461 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8521 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8581 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     8641 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8701 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8761 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8821 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8881 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8941 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     9001 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     9061 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9301 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     9361 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     9421 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     9481 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     9541 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     9601 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     9661 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     9721 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     9781 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     9841 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     9901 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9961 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    10021 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10081 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    10141 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10201 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10261 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10321 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10381 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10441 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10501 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10561 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10621 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10681 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10741 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10801 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10921 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10981 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    11041 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11101 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11221 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11341 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11401 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11461 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11521 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11581 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11641 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11701 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11761 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11821 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11881 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11941 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12001 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12061 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12301 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    12361 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    12421 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    12481 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    12541 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
    12601 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
    12661 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    12721 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    12781 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    12841 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    12901 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgg
//
