LOCUS       Exported               11985 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     regulatory      2427..2436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gene            2433..5390
                     /product="DpCasX_inactive"
                     /label=DpCasX_inactive
     variation       4446..4448
                     /label=D672A
     misc_feature    4737..4739
                     /label=E769A
     misc_feature    5235..5237
                     /label=D935A
     CDS             5391..5438
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5439..5462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5496..5690
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB)
                     transcriptionalrepression domain from the human zinc finger
                     protein ZNF10 (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5757..5777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5790..5846
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5847..6443
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6459..7047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6930..6941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7119..7352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7384..7608
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7654..8082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8096..8425
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8276..8411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8473..8520
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8539..8913
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9043..9164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9213..9229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9237..9253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9261..9291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9306..9327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9615..10203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10374..11234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11235..11339)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11605..11984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatggaaaa gagaatcaac aagatcagga
     2461 agaagctgag cgccgacaac gccaccaagc ctgtgtctag gagtggcccc atgaaaaccc
     2521 tgctcgtcag agtgatgacc gacgacctga agaagagact ggaaaagagg aggaagaaac
     2581 ccgaagtgat gccccaagtg atcagcaaca acgccgccaa caacctgaga atgctgctgg
     2641 acgactacac caagatgaag gaagccatcc tgcaggtcta ctggcaagag ttcaaggacg
     2701 accacgtggg cctgatgtgc aagtttgccc agcctgccag caagaagatc gaccagaaca
     2761 agctgaagcc cgagatggac gagaagggca atctgaccac agccggcttt gcctgctctc
     2821 agtgtggcca gcctctgttc gtgtacaagc tggaacaggt gtccgagaaa ggcaaggcct
     2881 acaccaacta cttcggcaga tgtaacgtgg ccgagcacga gaagctgatt ctgctggccc
     2941 agctgaaacc tgagaaggac tctgatgagg ccgtgaccta cagcctgggc aagtttggac
     3001 agagagccct ggacttctac agcatccacg tgaccaaaga aagcacacac cccgtgaagc
     3061 ccctggctca gatcgccggc aatagatacg cctctggacc tgtgggcaaa gccctgtccg
     3121 atgcctgcat gggaacaatc gccagcttcc tgagcaagta ccaggacatc atcatcgagc
     3181 accagaaggt ggtcaagggc aaccagaaga gactggaaag cctgagggag ctggccggca
     3241 aagagaacct ggaatacccc agcgtgaccc tgcctcctca gcctcacaca aaagaaggcg
     3301 tggacgccta caacgaagtg atcgccagag tgagaatgtg ggtcaacctg aacctgtggc
     3361 agaagctgaa actgtccagg gacgacgcca agcctctgct gagactgaag ggcttcccta
     3421 gcttccccgt ggtggaaaga agggagaatg aggtggactg gtggaacacc atcaacgaag
     3481 tcaagaaact gatcgatgcc aagagagaca tgggcagagt gttttggagc ggagtgaccg
     3541 ccgagaagag aaacaccatc ctggaaggct acaactacct gcctaacgag aacgaccaca
     3601 agaagagaga gggcagcctg gaaaacccta agaagcccgc caagagacag ttcggcgacc
     3661 tgctgctgta cctggagaag aagtatgccg gcgactgggg caaagtgttc gatgaggcct
     3721 gggagagaat cgacaagaag attgccggcc tgaccagcca catcgagaga gaggaagcca
     3781 gaaacgccga ggacgcccag tctaaagccg tgctgacaga ttggctgaga gccaaggcca
     3841 gcttcgtgct ggaaaggctg aaagaaatgg atgagaaaga gttctacgcc tgcgagatcc
     3901 agctgcagaa gtggtacggg gacctgagag gcaatccctt tgccgtggaa gccgagaaca
     3961 gagtggtgga catcagcggc tttagcatcg gctctgacgg ccactccatc cagtacagga
     4021 acctgctggc ctggaagtac ctggaaaatg gcaagagaga gttctacctg ctgatgaact
     4081 acggcaagaa gggcagaatc agattcaccg acggcaccga catcaagaaa agcggcaagt
     4141 ggcagggcct gctgtatggc ggcggaaagg ccaaagtgat cgacctgacc ttcgatcccg
     4201 acgacgagca gctgatcatt ctgcctctgg ccttcggcac cagacagggc agagagttca
     4261 tctggaacga tctgctgtcc ctggaaaccg gcctgatcaa gctggccaac ggcagggtga
     4321 tcgagaaaac catctacaac aagaaaatcg gcagagatga gcccgcactg tttgtggccc
     4381 tgacctttga gaggagagaa gtggtcgatc ccagcaacat caagcccgtg aacctgatcg
     4441 gagtggccag aggcgagaat atccctgccg tgattgccct gacagaccct gagggatgtc
     4501 ccctgcctga gtttaaggat agctctggcg gccctaccga catcctgaga atcggcgagg
     4561 gctacaaaga gaagcagaga gccatccagg ccgccaaaga ggtggaacag agaagagccg
     4621 gcggatacag cagaaagttc gccagcaagt ccagaaacct ggccgatgac atggtcagga
     4681 acagcgccag ggacctgttc taccacgccg tgacacatga tgccgtgctg gtgttcgcca
     4741 acctgtcaag aggcttcggc agacagggaa aaagaacctt catgaccgag agacagtaca
     4801 cgaagatgga agattggctc accgccaaac tggcctacga gggcctgaca agcaagacct
     4861 acctgtccaa gacactggcc cagtacacct ccaagacctg ctccaattgc ggcttcacca
     4921 tcaccaccgc cgactacgat ggaatgctcg tgagactgaa gaaaacctcc gacggctggg
     4981 ccaccacact gaacaacaaa gagctgaaag ccgagggcca gatcacctac tacaacagat
     5041 acaagaggca gaccgtcgag aaagagctgt ccgccgaact cgacagactg agcgaggaat
     5101 ccggcaacaa cgacatcagc aagtggacca agggacgcag agatgaggcc ctgttcctgc
     5161 tgaagaagag gttcagccac agacctgtgc aagagcagtt tgtgtgcctg gattgcggcc
     5221 acgaagtgca cgcagccgaa caggccgctc tgaatatcgc cagaagctgg ctgttcctga
     5281 acagcaacag caccgagttc aagagctaca agagcggcaa gcagcctttc gttggagcct
     5341 ggcaggcctt ctacaagaga aggctcaaag aagtgtggaa gcccaacgcc aagcgacctg
     5401 ccgccacaaa gaaggctgga caggctaaga agaagaaaga ttacaaagac gatgacgata
     5461 agggatccga tgctaagtca ctgactgcct ggtcccggac actggtgacc ttcaaggatg
     5521 tgtttgtgga cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcctgt
     5581 acagaaatgt gatgctggag aactataaga acctggtttc cttgggttat cagcttacta
     5641 agccagatgt gatcctccgg ttggagaagg gagaagagcc ctggctggtg gagagagaaa
     5701 ttcaccaaga gacccatcct gattcagaga ctgcatttga aatcaaatca tcagttccga
     5761 aaaagaaacg caaagttgct agctccggcg caacaaactt ctctctgctg aaacaagccg
     5821 gagatgtcga agagaatcct ggaccgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5881 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5941 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6001 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6061 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6121 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6181 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg
     6241 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6301 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6361 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6421 tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     6481 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6541 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6601 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6661 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6721 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6781 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6841 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6901 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6961 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7021 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     7081 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7141 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7201 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7261 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7321 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7381 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7441 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7501 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7561 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7621 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8101 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8161 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8221 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8341 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8401 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8461 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     8521 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     8581 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     8641 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     8701 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     8761 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     8821 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     8881 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     8941 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9001 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    11401 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    11461 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    11521 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    11581 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
    11641 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    11701 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    11761 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    11821 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    11881 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    11941 ctacttggca gtacatctac gtattagtca tcgctattac catgg
//
