LOCUS       Exported               11961 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11961
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     gene            2433..5366
                     /product="PxCasX_inactive"
                     /label=PxCasX_inactive
     variation       4407..4409
                     /label=D659A
     variation       4698..4700
                     /label=E756A
     misc_feature    5196..5198
                     /label=D922A
     CDS             5367..5414
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5415..5438
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5472..5666
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB)
                     transcriptionalrepression domain from the human zinc finger
                     protein ZNF10 (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5733..5753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5766..5822
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5823..6419
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6435..7023
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6906..6917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7095..7328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7360..7584
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7630..8058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8072..8401
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8252..8387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8449..8496
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8515..8889
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9019..9140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9189..9205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9213..9229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9237..9267)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9282..9303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9591..10179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10350..11210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11211..11315)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11581..11960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatgcaaga gatcaagaga atcaacaaga
     2461 tcagaaggag actggtcaag gacagcaaca caaagaaggc cggcaagaca ggccccatga
     2521 aaaccctgct cgtcagagtg atgacccctg acctgagaga gcggctggaa aacctgagaa
     2581 agaagcccga gaacatccct cagcctatca gcaacaccag cagggccaac ctgaacaagc
     2641 tgctgaccga ctacaccgag atgaagaaag ccatcctgca cgtgtactgg gaagagttcc
     2701 agaaagaccc cgtgggcctg atgagcagag ttgctcagcc cgctcctaag aacatcgacc
     2761 agagaaagct gatccccgtg aaggacggca acgagagact gacctctagc ggctttgcct
     2821 gcagccagtg ttgccagcct ctgtacgtgt acaagctgga acaagtgaac gacaagggca
     2881 agccccacac caactacttc ggcagatgca acgtgtccga gcacgagagg ctgatcctgc
     2941 tgtctcctca caagcccgag gccaacgatg agctggtcac atacagcctg ggcaagttcg
     3001 gacagagagc cctggacttc tacagcatcc acgtgaccag ggagagcaat caccctgtga
     3061 agcccctgga acagatcggc ggcaatagct gtgcctctgg acctgtggga aaagccctga
     3121 gcgacgcctg tatgggagcc gtggcatcct tcctgaccaa gtaccaggac atcatcctgg
     3181 aacaccagaa agtgatcaag aagaacgaga aaagactggc caacctcaag gatatcgcca
     3241 gcgctaacgg cctggccttt cctaagatca ccctgcctcc acagcctcac accaaagagg
     3301 gcatcgaggc ctacaacaac gtggtggccc agatcgtgat ttgggtcaac ctgaatctgt
     3361 ggcagaagct gaagatcggc agggacgaag ccaagccact gcagagactg aagggcttcc
     3421 ctagcttccc tctggtggaa agacaggcca atgaagtgga ttggtgggac atggtctgca
     3481 acgtgaagaa gctgatcaac gagaagaaag aggatggcaa ggttttctgg cagaacctgg
     3541 ccggctacaa gagacaagaa gccctgctgc cttacctgag cagcgaagag gaccggaaga
     3601 agggcaagaa gttcgccaga taccagttcg gcgacctgct gctgcacctg gaaaagaagc
     3661 acggcgagga ctggggcaaa gtgtacgatg aggcctggga gagaatcgac aagaaggtgg
     3721 aaggcctgag caagcacatt aagctggaag aggaaagaag gagcgaggac gcccaatcta
     3781 aagccgctct gaccgattgg ctgagagcca aggccagctt tgtgatcgag ggcctgaaag
     3841 aggccgacaa ggacgagttc tgcagatgcg agctgaagct gcagaagtgg tacggcgatc
     3901 tgagaggcaa gcccttcgcc attgaggccg agaacagcat cctggacatc agcggcttca
     3961 gcaagcagta caactgcgcc ttcatttggc agaaagacgg cgtcaagaaa ctgaacctgt
     4021 acctgatcat caattacttc aaaggcggca agctgcggtt caagaagatc aaacccgagg
     4081 ccttcgaggc taacagattc tacaccgtga tcaacaaaaa gtccggcgag atcgtgccca
     4141 tggaagtgaa cttcaacttc gacgacccca acctgattat cctgcctctg gccttcggca
     4201 agagacaggg cagagagttc atctggaacg atctgctgag cctggaaacc ggctctctga
     4261 agctggccaa tggcagagtg atcgagaaaa ccctgtacaa caggagaacc agacaggacg
     4321 agcctgctct gtttgtggcc ctgaccttcg agagaagaga ggtgctggac agcagcaaca
     4381 tcaagcccat gaacctgatc ggcatcgccc ggggcgagaa tatccctgct gtgatcgccc
     4441 tgacagaccc tgaaggatgc ccactgagca gattcaagga ctccctgggc aaccctacac
     4501 acatcctgag aatcggcgag agctacaaag agaagcagag gacaatccag gccgccaaag
     4561 aggtggaaca gagaagagcc ggcggatact ctaggaagta cgccagcaag gccaagaatc
     4621 tggccgacga catggtccga aacaccgcca gagatctgct gtactacgcc gtgacacagg
     4681 acgccatgct gatcttcgcc aatctgagca gaggcttcgg ccggcagggc aagagaacct
     4741 ttatggccga gaggcagtac accagaatgg aagattggct cacagctaaa ctggcctacg
     4801 agggactgcc cagcaagacc tacctgtcca aaacactggc ccagtatacc tccaagacct
     4861 gcagcaattg cggcttcacc atcaccagcg ccgactacga cagagtgctg gaaaagctca
     4921 agaaaaccgc caccggctgg atgaccacca tcaacggcaa agagctgaag gttgagggcc
     4981 agatcaccta ctacaacagg tacaagaggc agaacgtcgt gaaggatctg agcgtggaac
     5041 tggacagact gagcgaagag agcgtgaaca acgacatcag cagctggaca aagggcagat
     5101 caggcgaggc tctgagcctg ctgaagaaga ggtttagcca cagacctgtg caagagaagt
     5161 tcgtgtgcct gaactgcggc ttcgagacac acgccgccga acaggctgcc ctgaacattg
     5221 ccagaagctg gctgttcctg agaagccaag agtacaagaa gtaccagacc aacaagacca
     5281 ccggcaacac cgacaagagg gcctttgtgg aaacctggca gagcttctac agaaaaaagc
     5341 tgaaagaagt ctggaagccc gccgtgaagc gacctgccgc cacaaagaag gctggacagg
     5401 ctaagaagaa gaaagattac aaagacgatg acgataaggg atccgatgct aagtcactga
     5461 ctgcctggtc ccggacactg gtgaccttca aggatgtgtt tgtggacttc accagggagg
     5521 agtggaagct gctggacact gctcagcaga tcctgtacag aaatgtgatg ctggagaact
     5581 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg
     5641 agaagggaga agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt
     5701 cagagactgc atttgaaatc aaatcatcag ttccgaaaaa gaaacgcaaa gttgctagct
     5761 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     5821 cgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     5881 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     5941 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6001 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6061 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6121 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6181 ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca
     6241 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6301 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6361 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     6421 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6481 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6541 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6601 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6661 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6721 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6781 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6841 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6901 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6961 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7021 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7081 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7141 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7201 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7261 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7321 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7381 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7441 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7501 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7561 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     7621 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7681 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7741 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7801 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7861 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7921 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7981 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8041 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8101 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8161 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8221 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8341 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8401 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     8461 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     8521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     8581 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     8641 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     8701 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     8761 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     8821 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     8881 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8941 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9001 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9121 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9181 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9241 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9301 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9361 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9841 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10021 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10261 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10321 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10381 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10441 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10501 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10561 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10621 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10681 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10741 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10801 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10861 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10921 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10981 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11041 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11101 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11161 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11221 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11281 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11341 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
    11401 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
    11461 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
    11521 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
    11581 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    11641 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    11701 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    11761 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    11821 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    11881 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    11941 tagtcatcgc tattaccatg g
//
