LOCUS       Exported               14295 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14295)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14295
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             2433..6353
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      6073..6082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6354..6401
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6402..6425
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6468..6492
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6522..6671
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activationdomain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6696..6716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7146..7502
                     /codon_start=1
                     /product="transcriptional activation domain of human
                     RelA,also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7521..8090
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             8100..8156
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8100..8113
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNF"
     CDS             8157..8753
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8769..9357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(9240..9251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9429..9662
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9694..9918
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9964..10392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10406..10735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10586..10721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10783..10830
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10849..11223
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11353..11474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11523..11539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11547..11563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11571..11601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11616..11637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11925..12513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12684..13544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13545..13649)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        13915..14294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatgacaca gttcgagggc tttaccaacc
     2461 tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc
     2521 acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc
     2581 tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc
     2641 agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg
     2701 agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact
     2761 tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca
     2821 agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga
     2881 ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct
     2941 ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc
     3001 cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac
     3061 gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg
     3121 gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc
     3181 tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag
     3241 gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg
     3301 agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc
     3361 tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga
     3421 tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg
     3481 aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga
     3541 agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt
     3601 atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc
     3661 agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg
     3721 agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc
     3781 tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc
     3841 agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca
     3901 acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt
     3961 ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga
     4021 agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg aataaggaga
     4081 agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa
     4141 agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct
     4201 ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca
     4261 cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca
     4321 acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga
     4381 aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca
     4441 gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga
     4501 caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt
     4561 actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg
     4621 agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact
     4681 ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt
     4741 ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc
     4801 gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga
     4861 agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg
     4921 tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga
     4981 tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct
     5041 ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc
     5101 agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgcccggg
     5161 gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc
     5221 ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg
     5281 agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg
     5341 gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg
     5401 tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg
     5461 ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg
     5521 actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca
     5581 cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata
     5641 catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga
     5701 atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa
     5761 ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc
     5821 ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca
     5881 agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca
     5941 ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg
     6001 gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc
     6061 acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg
     6121 ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg
     6181 actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca
     6241 tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc
     6301 agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaagcgac
     6361 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg
     6421 ataagggatc ctcgccaggg atccgtcgac ttgacgcgtt gatatcaaca agtttgtaca
     6481 aaaaagcagg ctacaaagag gccagcggtt ccggacgggc tgacgcattg gacgattttg
     6541 atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg
     6601 atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc
     6661 tggacatgct gattaactct agaagttccg gatctccgaa aaagaaacgc aaagttggta
     6721 gccagtacct gcccgacacc gacgaccggc accggatcga ggaaaagcgg aagcggacct
     6781 acgagacatt caagagcatc atgaagaagt cccccttcag cggccccacc gaccctagac
     6841 ctccacctag aagaatcgcc gtgcccagca gatccagcgc cagcgtgcca aaacctgccc
     6901 cccagcctta ccccttcacc agcagcctga gcaccatcaa ctacgacgag ttccctacca
     6961 tggtgttccc cagcggccag atctctcagg cctctgctct ggctccagcc cctcctcagg
     7021 tgctgcctca ggctcctgct cctgcaccag ctccagccat ggtgtctgca ctggctcagg
     7081 caccagcacc cgtgcctgtg ctggctcctg gacctccaca ggctgtggct ccaccagccc
     7141 ctaaacctac acaggccggc gagggcacac tgtctgaagc tctgctgcag ctgcagttcg
     7201 acgacgagga tctgggagcc ctgctgggaa acagcaccga tcctgccgtg ttcaccgacc
     7261 tggccagcgt ggacaacagc gagttccagc agctgctgaa ccagggcatc cctgtggccc
     7321 ctcacaccac cgagcccatg ctgatggaat accccgaggc catcacccgg ctcgtgacag
     7381 gcgctcagag gcctcctgat ccagctcctg cccctctggg agcaccaggc ctgcctaatg
     7441 gactgctgtc tggcgacgag gacttcagct ctatcgccga tatggatttc tcagccttgc
     7501 tgggctctgg cagcggcagc cgggattcca gggaagggat gtttttgccg aagcctgagg
     7561 ccggctccgc tattagtgac gtgtttgagg gccgcgaggt gtgccagcca aaacgaatcc
     7621 ggccatttca tcctccagga agtccatggg ccaaccgccc actccccgcc agcctcgcac
     7681 caacaccaac cggtccagta catgagccag tcgggtcact gaccccggca ccagtccctc
     7741 agccactgga tccagcgccc gcagtgactc ccgaggccag tcacctgttg gaggatcccg
     7801 atgaagagac gagccaggct gtcaaagccc ttcgggagat ggccgatact gtgattcccc
     7861 agaaggaaga ggctgcaatc tgtggccaaa tggacctttc ccatccgccc ccaaggggcc
     7921 atctggatga gctgacaacc acacttgagt ccatgaccga ggatctgaac ctggactcac
     7981 ccctgacccc ggaattgaac gagattctgg ataccttcct gaacgacgag tgcctcttgc
     8041 atgccatgca tatcagcaca ggactgtcca tcttcgacac atctctgttt ggatccggcg
     8101 caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgaccg
     8161 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     8221 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     8281 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8341 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     8401 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8461 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8521 ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8581 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8641 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     8701 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt
     8761 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8821 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8881 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8941 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9001 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9061 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9121 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9181 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9241 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9301 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     9361 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9421 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     9481 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9541 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9601 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9661 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     9721 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9781 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9841 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     9901 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     9961 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10021 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10081 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10141 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10201 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10261 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10321 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10381 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    10441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    10501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10561 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    10681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10741 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    10801 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
    10861 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    10921 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    10981 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    11041 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    11101 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    11161 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    11221 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    11281 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    11341 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11461 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    11521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    11581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    13021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    13081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    13141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    13201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    13261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    13321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    13381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    13441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13681 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    13741 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    13801 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    13861 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    13921 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    13981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    14041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    14101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    14161 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    14221 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    14281 tcgctattac catgg
//
