LOCUS       Exported               13332 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13332)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13332
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     regulatory      2427..2436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gene            2433..5390
                     /product="DpCasX_inactive"
                     /label=DpCasX_inactive
     variation       4446..4448
                     /label=D672A
     misc_feature    4737..4739
                     /label=E769A
     misc_feature    5235..5237
                     /label=D935A
     CDS             5391..5438
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5439..5462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5559..5708
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activationdomain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5733..5753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6183..6539
                     /codon_start=1
                     /product="transcriptional activation domain of human
                     RelA,also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6558..7127
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             7137..7150
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNF"
     CDS             7194..7790
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7806..8394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(8277..8288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8466..8699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8731..8955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9001..9429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9443..9772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9623..9758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9820..9867
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9886..10260
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10390..10511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10584..10600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10608..10638)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10653..10674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10962..11550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11721..12581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12582..12686)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        12952..13331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatggaaaa gagaatcaac aagatcagga
     2461 agaagctgag cgccgacaac gccaccaagc ctgtgtctag gagtggcccc atgaaaaccc
     2521 tgctcgtcag agtgatgacc gacgacctga agaagagact ggaaaagagg aggaagaaac
     2581 ccgaagtgat gccccaagtg atcagcaaca acgccgccaa caacctgaga atgctgctgg
     2641 acgactacac caagatgaag gaagccatcc tgcaggtcta ctggcaagag ttcaaggacg
     2701 accacgtggg cctgatgtgc aagtttgccc agcctgccag caagaagatc gaccagaaca
     2761 agctgaagcc cgagatggac gagaagggca atctgaccac agccggcttt gcctgctctc
     2821 agtgtggcca gcctctgttc gtgtacaagc tggaacaggt gtccgagaaa ggcaaggcct
     2881 acaccaacta cttcggcaga tgtaacgtgg ccgagcacga gaagctgatt ctgctggccc
     2941 agctgaaacc tgagaaggac tctgatgagg ccgtgaccta cagcctgggc aagtttggac
     3001 agagagccct ggacttctac agcatccacg tgaccaaaga aagcacacac cccgtgaagc
     3061 ccctggctca gatcgccggc aatagatacg cctctggacc tgtgggcaaa gccctgtccg
     3121 atgcctgcat gggaacaatc gccagcttcc tgagcaagta ccaggacatc atcatcgagc
     3181 accagaaggt ggtcaagggc aaccagaaga gactggaaag cctgagggag ctggccggca
     3241 aagagaacct ggaatacccc agcgtgaccc tgcctcctca gcctcacaca aaagaaggcg
     3301 tggacgccta caacgaagtg atcgccagag tgagaatgtg ggtcaacctg aacctgtggc
     3361 agaagctgaa actgtccagg gacgacgcca agcctctgct gagactgaag ggcttcccta
     3421 gcttccccgt ggtggaaaga agggagaatg aggtggactg gtggaacacc atcaacgaag
     3481 tcaagaaact gatcgatgcc aagagagaca tgggcagagt gttttggagc ggagtgaccg
     3541 ccgagaagag aaacaccatc ctggaaggct acaactacct gcctaacgag aacgaccaca
     3601 agaagagaga gggcagcctg gaaaacccta agaagcccgc caagagacag ttcggcgacc
     3661 tgctgctgta cctggagaag aagtatgccg gcgactgggg caaagtgttc gatgaggcct
     3721 gggagagaat cgacaagaag attgccggcc tgaccagcca catcgagaga gaggaagcca
     3781 gaaacgccga ggacgcccag tctaaagccg tgctgacaga ttggctgaga gccaaggcca
     3841 gcttcgtgct ggaaaggctg aaagaaatgg atgagaaaga gttctacgcc tgcgagatcc
     3901 agctgcagaa gtggtacggg gacctgagag gcaatccctt tgccgtggaa gccgagaaca
     3961 gagtggtgga catcagcggc tttagcatcg gctctgacgg ccactccatc cagtacagga
     4021 acctgctggc ctggaagtac ctggaaaatg gcaagagaga gttctacctg ctgatgaact
     4081 acggcaagaa gggcagaatc agattcaccg acggcaccga catcaagaaa agcggcaagt
     4141 ggcagggcct gctgtatggc ggcggaaagg ccaaagtgat cgacctgacc ttcgatcccg
     4201 acgacgagca gctgatcatt ctgcctctgg ccttcggcac cagacagggc agagagttca
     4261 tctggaacga tctgctgtcc ctggaaaccg gcctgatcaa gctggccaac ggcagggtga
     4321 tcgagaaaac catctacaac aagaaaatcg gcagagatga gcccgcactg tttgtggccc
     4381 tgacctttga gaggagagaa gtggtcgatc ccagcaacat caagcccgtg aacctgatcg
     4441 gagtggccag aggcgagaat atccctgccg tgattgccct gacagaccct gagggatgtc
     4501 ccctgcctga gtttaaggat agctctggcg gccctaccga catcctgaga atcggcgagg
     4561 gctacaaaga gaagcagaga gccatccagg ccgccaaaga ggtggaacag agaagagccg
     4621 gcggatacag cagaaagttc gccagcaagt ccagaaacct ggccgatgac atggtcagga
     4681 acagcgccag ggacctgttc taccacgccg tgacacatga tgccgtgctg gtgttcgcca
     4741 acctgtcaag aggcttcggc agacagggaa aaagaacctt catgaccgag agacagtaca
     4801 cgaagatgga agattggctc accgccaaac tggcctacga gggcctgaca agcaagacct
     4861 acctgtccaa gacactggcc cagtacacct ccaagacctg ctccaattgc ggcttcacca
     4921 tcaccaccgc cgactacgat ggaatgctcg tgagactgaa gaaaacctcc gacggctggg
     4981 ccaccacact gaacaacaaa gagctgaaag ccgagggcca gatcacctac tacaacagat
     5041 acaagaggca gaccgtcgag aaagagctgt ccgccgaact cgacagactg agcgaggaat
     5101 ccggcaacaa cgacatcagc aagtggacca agggacgcag agatgaggcc ctgttcctgc
     5161 tgaagaagag gttcagccac agacctgtgc aagagcagtt tgtgtgcctg gattgcggcc
     5221 acgaagtgca cgcagccgaa caggccgctc tgaatatcgc cagaagctgg ctgttcctga
     5281 acagcaacag caccgagttc aagagctaca agagcggcaa gcagcctttc gttggagcct
     5341 ggcaggcctt ctacaagaga aggctcaaag aagtgtggaa gcccaacgcc aagcgacctg
     5401 ccgccacaaa gaaggctgga caggctaaga agaagaaaga ttacaaagac gatgacgata
     5461 agggatcctc gccagggatc cgtcgacttg acgcgttgat atcaacaagt ttgtacaaaa
     5521 aagcaggcta caaagaggcc agcggttccg gacgggctga cgcattggac gattttgatc
     5581 tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg
     5641 cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg
     5701 acatgctgat taactctaga agttccggat ctccgaaaaa gaaacgcaaa gttggtagcc
     5761 agtacctgcc cgacaccgac gaccggcacc ggatcgagga aaagcggaag cggacctacg
     5821 agacattcaa gagcatcatg aagaagtccc ccttcagcgg ccccaccgac cctagacctc
     5881 cacctagaag aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa cctgcccccc
     5941 agccttaccc cttcaccagc agcctgagca ccatcaacta cgacgagttc cctaccatgg
     6001 tgttccccag cggccagatc tctcaggcct ctgctctggc tccagcccct cctcaggtgc
     6061 tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg gctcaggcac
     6121 cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca ccagccccta
     6181 aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg cagttcgacg
     6241 acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc accgacctgg
     6301 ccagcgtgga caacagcgag ttccagcagc tgctgaacca gggcatccct gtggcccctc
     6361 acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc gtgacaggcg
     6421 ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg cctaatggac
     6481 tgctgtctgg cgacgaggac ttcagctcta tcgccgatat ggatttctca gccttgctgg
     6541 gctctggcag cggcagccgg gattccaggg aagggatgtt tttgccgaag cctgaggccg
     6601 gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg ccagccaaaa cgaatccggc
     6661 catttcatcc tccaggaagt ccatgggcca accgcccact ccccgccagc ctcgcaccaa
     6721 caccaaccgg tccagtacat gagccagtcg ggtcactgac cccggcacca gtccctcagc
     6781 cactggatcc agcgcccgca gtgactcccg aggccagtca cctgttggag gatcccgatg
     6841 aagagacgag ccaggctgtc aaagcccttc gggagatggc cgatactgtg attccccaga
     6901 aggaagaggc tgcaatctgt ggccaaatgg acctttccca tccgccccca aggggccatc
     6961 tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg gactcacccc
     7021 tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc ctcttgcatg
     7081 ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgtttgga tccggcgcaa
     7141 caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgaccgagt
     7201 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     7261 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     7321 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7381 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7441 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7501 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7561 tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7621 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7681 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7741 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttaag
     7801 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7861 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7921 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7981 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8041 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8101 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8161 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8221 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8281 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8341 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     8401 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8461 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8701 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8761 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8821 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8881 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8941 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     9001 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     9061 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     9121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     9241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     9361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     9721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9781 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     9841 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     9901 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     9961 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
    10021 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
    10081 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
    10141 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
    10201 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
    10261 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10381 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10501 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    10561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    10801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    11041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    11101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    11581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    11641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    12001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    12061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    12301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    12721 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    12781 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    12841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    12901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    12961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    13021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    13081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    13141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    13201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    13261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    13321 ctattaccat gg
//
