LOCUS       Exported               13308 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13308)
  AUTHORS   Mario Escobar
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13308
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     gene            2433..5366
                     /product="PxCasX_inactive"
                     /label=PxCasX_inactive
     variation       4407..4409
                     /label=D659A
     variation       4698..4700
                     /label=E756A
     misc_feature    5196..5198
                     /label=D922A
     CDS             5367..5414
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5415..5438
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5535..5684
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activationdomain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5709..5729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6159..6515
                     /codon_start=1
                     /product="transcriptional activation domain of human
                     RelA,also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6534..7103
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             7113..7169
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7170..7766
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7782..8370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(8253..8264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8442..8675
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8707..8931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8977..9405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9419..9748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9599..9734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9796..9843
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9862..10236
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10366..10487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10560..10576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10584..10614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10629..10650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10938..11526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11697..12557)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12558..12662)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        12928..13307
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2041 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2101 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct
     2161 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacagga ccggttctag agcgctgcca ccatgcaaga gatcaagaga atcaacaaga
     2461 tcagaaggag actggtcaag gacagcaaca caaagaaggc cggcaagaca ggccccatga
     2521 aaaccctgct cgtcagagtg atgacccctg acctgagaga gcggctggaa aacctgagaa
     2581 agaagcccga gaacatccct cagcctatca gcaacaccag cagggccaac ctgaacaagc
     2641 tgctgaccga ctacaccgag atgaagaaag ccatcctgca cgtgtactgg gaagagttcc
     2701 agaaagaccc cgtgggcctg atgagcagag ttgctcagcc cgctcctaag aacatcgacc
     2761 agagaaagct gatccccgtg aaggacggca acgagagact gacctctagc ggctttgcct
     2821 gcagccagtg ttgccagcct ctgtacgtgt acaagctgga acaagtgaac gacaagggca
     2881 agccccacac caactacttc ggcagatgca acgtgtccga gcacgagagg ctgatcctgc
     2941 tgtctcctca caagcccgag gccaacgatg agctggtcac atacagcctg ggcaagttcg
     3001 gacagagagc cctggacttc tacagcatcc acgtgaccag ggagagcaat caccctgtga
     3061 agcccctgga acagatcggc ggcaatagct gtgcctctgg acctgtggga aaagccctga
     3121 gcgacgcctg tatgggagcc gtggcatcct tcctgaccaa gtaccaggac atcatcctgg
     3181 aacaccagaa agtgatcaag aagaacgaga aaagactggc caacctcaag gatatcgcca
     3241 gcgctaacgg cctggccttt cctaagatca ccctgcctcc acagcctcac accaaagagg
     3301 gcatcgaggc ctacaacaac gtggtggccc agatcgtgat ttgggtcaac ctgaatctgt
     3361 ggcagaagct gaagatcggc agggacgaag ccaagccact gcagagactg aagggcttcc
     3421 ctagcttccc tctggtggaa agacaggcca atgaagtgga ttggtgggac atggtctgca
     3481 acgtgaagaa gctgatcaac gagaagaaag aggatggcaa ggttttctgg cagaacctgg
     3541 ccggctacaa gagacaagaa gccctgctgc cttacctgag cagcgaagag gaccggaaga
     3601 agggcaagaa gttcgccaga taccagttcg gcgacctgct gctgcacctg gaaaagaagc
     3661 acggcgagga ctggggcaaa gtgtacgatg aggcctggga gagaatcgac aagaaggtgg
     3721 aaggcctgag caagcacatt aagctggaag aggaaagaag gagcgaggac gcccaatcta
     3781 aagccgctct gaccgattgg ctgagagcca aggccagctt tgtgatcgag ggcctgaaag
     3841 aggccgacaa ggacgagttc tgcagatgcg agctgaagct gcagaagtgg tacggcgatc
     3901 tgagaggcaa gcccttcgcc attgaggccg agaacagcat cctggacatc agcggcttca
     3961 gcaagcagta caactgcgcc ttcatttggc agaaagacgg cgtcaagaaa ctgaacctgt
     4021 acctgatcat caattacttc aaaggcggca agctgcggtt caagaagatc aaacccgagg
     4081 ccttcgaggc taacagattc tacaccgtga tcaacaaaaa gtccggcgag atcgtgccca
     4141 tggaagtgaa cttcaacttc gacgacccca acctgattat cctgcctctg gccttcggca
     4201 agagacaggg cagagagttc atctggaacg atctgctgag cctggaaacc ggctctctga
     4261 agctggccaa tggcagagtg atcgagaaaa ccctgtacaa caggagaacc agacaggacg
     4321 agcctgctct gtttgtggcc ctgaccttcg agagaagaga ggtgctggac agcagcaaca
     4381 tcaagcccat gaacctgatc ggcatcgccc ggggcgagaa tatccctgct gtgatcgccc
     4441 tgacagaccc tgaaggatgc ccactgagca gattcaagga ctccctgggc aaccctacac
     4501 acatcctgag aatcggcgag agctacaaag agaagcagag gacaatccag gccgccaaag
     4561 aggtggaaca gagaagagcc ggcggatact ctaggaagta cgccagcaag gccaagaatc
     4621 tggccgacga catggtccga aacaccgcca gagatctgct gtactacgcc gtgacacagg
     4681 acgccatgct gatcttcgcc aatctgagca gaggcttcgg ccggcagggc aagagaacct
     4741 ttatggccga gaggcagtac accagaatgg aagattggct cacagctaaa ctggcctacg
     4801 agggactgcc cagcaagacc tacctgtcca aaacactggc ccagtatacc tccaagacct
     4861 gcagcaattg cggcttcacc atcaccagcg ccgactacga cagagtgctg gaaaagctca
     4921 agaaaaccgc caccggctgg atgaccacca tcaacggcaa agagctgaag gttgagggcc
     4981 agatcaccta ctacaacagg tacaagaggc agaacgtcgt gaaggatctg agcgtggaac
     5041 tggacagact gagcgaagag agcgtgaaca acgacatcag cagctggaca aagggcagat
     5101 caggcgaggc tctgagcctg ctgaagaaga ggtttagcca cagacctgtg caagagaagt
     5161 tcgtgtgcct gaactgcggc ttcgagacac acgccgccga acaggctgcc ctgaacattg
     5221 ccagaagctg gctgttcctg agaagccaag agtacaagaa gtaccagacc aacaagacca
     5281 ccggcaacac cgacaagagg gcctttgtgg aaacctggca gagcttctac agaaaaaagc
     5341 tgaaagaagt ctggaagccc gccgtgaagc gacctgccgc cacaaagaag gctggacagg
     5401 ctaagaagaa gaaagattac aaagacgatg acgataaggg atcctcgcca gggatccgtc
     5461 gacttgacgc gttgatatca acaagtttgt acaaaaaagc aggctacaaa gaggccagcg
     5521 gttccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc
     5581 tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca
     5641 tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac tctagaagtt
     5701 ccggatctcc gaaaaagaaa cgcaaagttg gtagccagta cctgcccgac accgacgacc
     5761 ggcaccggat cgaggaaaag cggaagcgga cctacgagac attcaagagc atcatgaaga
     5821 agtccccctt cagcggcccc accgacccta gacctccacc tagaagaatc gccgtgccca
     5881 gcagatccag cgccagcgtg ccaaaacctg ccccccagcc ttaccccttc accagcagcc
     5941 tgagcaccat caactacgac gagttcccta ccatggtgtt ccccagcggc cagatctctc
     6001 aggcctctgc tctggctcca gcccctcctc aggtgctgcc tcaggctcct gctcctgcac
     6061 cagctccagc catggtgtct gcactggctc aggcaccagc acccgtgcct gtgctggctc
     6121 ctggacctcc acaggctgtg gctccaccag cccctaaacc tacacaggcc ggcgagggca
     6181 cactgtctga agctctgctg cagctgcagt tcgacgacga ggatctggga gccctgctgg
     6241 gaaacagcac cgatcctgcc gtgttcaccg acctggccag cgtggacaac agcgagttcc
     6301 agcagctgct gaaccagggc atccctgtgg cccctcacac caccgagccc atgctgatgg
     6361 aataccccga ggccatcacc cggctcgtga caggcgctca gaggcctcct gatccagctc
     6421 ctgcccctct gggagcacca ggcctgccta atggactgct gtctggcgac gaggacttca
     6481 gctctatcgc cgatatggat ttctcagcct tgctgggctc tggcagcggc agccgggatt
     6541 ccagggaagg gatgtttttg ccgaagcctg aggccggctc cgctattagt gacgtgtttg
     6601 agggccgcga ggtgtgccag ccaaaacgaa tccggccatt tcatcctcca ggaagtccat
     6661 gggccaaccg cccactcccc gccagcctcg caccaacacc aaccggtcca gtacatgagc
     6721 cagtcgggtc actgaccccg gcaccagtcc ctcagccact ggatccagcg cccgcagtga
     6781 ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga gacgagccag gctgtcaaag
     6841 cccttcggga gatggccgat actgtgattc cccagaagga agaggctgca atctgtggcc
     6901 aaatggacct ttcccatccg cccccaaggg gccatctgga tgagctgaca accacacttg
     6961 agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg aacgagattc
     7021 tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc acaggactgt
     7081 ccatcttcga cacatctctg tttggatccg gcgcaacaaa cttctctctg ctgaaacaag
     7141 ccggagatgt cgaagagaat cctggaccga ccgagtacaa gcccacggtg cgcctcgcca
     7201 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7261 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7321 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7381 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7441 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7501 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc
     7561 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7621 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7681 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7741 gcatgacccg caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca
     7801 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7861 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7921 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7981 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8041 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8101 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8161 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     8221 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8281 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8341 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     8401 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8461 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8641 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     8701 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8881 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8941 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     9001 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9061 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     9241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     9301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     9361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     9421 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9481 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9541 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9601 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9661 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     9721 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     9781 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     9841 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     9901 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     9961 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
    10021 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
    10081 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
    10141 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
    10201 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
    10261 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    10321 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    10381 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10441 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    10501 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    11401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    12001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    12061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    12121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    12181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    12241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    12301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12661 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    12721 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    12781 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    12841 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    12901 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
    12961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
    13021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    13081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    13141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    13201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    13261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgg
//
