LOCUS       Exported                5213 bp DNA     circular SYN 05-OCT-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
COMMENT     pOTTC1584 was constructed by Chris Richie at GEVVC/NIDA 
            (CR1805v2_E).
            
            backbone was pOTTC1512 was constructed by Chris Richie at GEVVC/NIDA
            (CR1776_D).
            backbone: pOTTC1505 was constructed by Chris Richie at GEVVC/NIDA 
            (CR1769_R).
            backbone:  pOTTC1491 was constructed by Chris Richie at GEVVC/NIDA 
            (CR1727_C).
FEATURES             Location/Qualifiers
     source          1..5213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..117
                     /label=scITR (AAV, OTTC trs deletion)
                     /note="color: #804000"
     promoter        144..732
                     /label=CMV-IE promoter
                     /note="color: #408080"
     CDS             752..1576
                     /label=secreted EGFP-THPKTEL
                     /note="color: #0080c0; direction: RIGHT"
     sig_peptide     752..835
                     /label=CDNF signal peptide [MWCASP...TQG-QE]
                     /note="color: #ff8000"
     CDS             836..1552
                     /label=eGFP
                     /note="color: #0080c0; direction: RIGHT"
     transit_peptide 1553..1573
                     /label=CDNF Tail [THPKTEL]
                     /note="color: #8080c0"
     misc_feature    1571..1573
                     /label=L161 - after signal peptide cleavage
                     /note="color: #00ffff"
     misc_RNA        1607..2215
                     /label=WPRE
                     /note="color: #800080; direction: RIGHT"
     misc_feature    2245..2459
                     /label=bGH polyA
                     /note="color: #ff8080"
     LTR             complement(2476..2616)
                     /label=ITR (AAV)
                     /note="color: #ff8000; direction: LEFT"
     rep_origin      2691..3146
                     /label=M13 origin
                     /note="color: #808080"
     rep_origin      2708..3014
                     /label=f1 origin
                     /note="color: #808080"
     promoter        3434..3532
                     /label=Bla promoter (prokaryotic)
                     /note="color: #804000"
     CDS             3533..4390
                     /label=AmpR
                     /note="color: #0080c0; direction: RIGHT"
     rep_origin      4488..5170
                     /label=ColE1 origin
                     /note="color: #808080"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggctg
      121 cggccgcacg cgtggatcta atatagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      721 aaccgtcaga tccgaagtta tcgctagcac catgtggtgc gcgagcccag ttgctgtggt
      781 ggccttttgc gccgggcttt tggtctctca cccggtgctg acgcagggcc aggagatggt
      841 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      901 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      961 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1021 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1081 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1141 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1201 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1261 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1321 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1441 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agacacaccc
     1561 caaaacagag ctctagggcg cgccataact tcgaattcga tatcaagctt atcgataatc
     1621 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1681 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1741 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1801 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1861 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     1921 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1981 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2041 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2101 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2161 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     2221 tgctcgagag atctaattcc tagaagcctc gactgtgcct tctagttgcc agccatctgt
     2281 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ccgtcctttc
     2341 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2401 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctggggc
     2461 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     2521 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     2581 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     2641 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     2701 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2761 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2821 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2881 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     2941 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3001 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3061 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3121 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3181 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3241 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3301 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3361 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3961 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4021 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4081 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4141 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4201 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4261 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4321 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4381 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4441 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4681 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
