LOCUS       pAAV-hSyn-G-Flamp1_mut        5761 bp ds-DNA     circular UNA 04-JUL-2022
DEFINITION  .
ACCESSION   urn.local...r-csnnog5
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2020 from SnapGene 4.1.9 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|932947200|                                                 
            VNTDBDATE|932947200|                                               
            VNTNAME|pAAV-hSyn-GFlamp1_mut.dna|.
FEATURES             Location/Qualifiers
     unsure          1..141
                     /note="/vntifkey=21"
                     /label="L-ITR"
     promoter        156..597
                     /label="synapsin"
     misc_feature    446..469
                     /label="ColonyF_hSyn"
     misc_feature    568..597
                     /label="336F_hSyn-G-Flamp2"
     CDS             598..1863
                     /codon_start=1
                     /modified_by="shu"
                     /label="G-Flamp1-R307E(G-Flamp1-mut)"
     misc_feature    1714..1716
                     /label="R_E mut"
     misc_feature    complement(1864..1898)
                     /label="337R_G-Flamp2-AAV"
     unsure          1878..2486
                     /note="/vntifkey=21"
                     /label="WPRE"
     misc_feature    1888
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1963..1980)
                     /label="pWPTRev"
     polyA_signal    2506..2984
                     /note="/vntifkey=25"
                     /label="hGH polyA"
     unsure          3024..3164
                     /note="/vntifkey=21"
                     /label="R-ITR"
     rep_origin      3256..3562
                     /note="/vntifkey=33"
                     /label="f1 Origin"
     unsure          4081..4938
                     /note="/vntifkey=21"
                     /label="AmpR"
     rep_origin      5089..5756
                     /note="/vntifkey=33"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 ggctcccacc accaccatca ccatggcatg gcaagcatga caggggggca gcagatggga
      661 cgggacctct atgatgatga cgacaaagat cccatgggct tctaccagga ggtgagaaga
      721 ggggactttg tgaggaactg gcagctagtg gcagctgtgc ctctcttcca gaagctgggc
      781 ccagccgtcc tggtagaaat tgtgagagcc ctgagagccc ggaccgttcc tgctggggct
      841 gtcatctgtc gcattggaga gccaggagac aggatgtttt tcgttgtgga aggcagtgtg
      901 agtgtggcta ccaactgggg caacgtctat atcacagccg acaagcagaa gaacggcatc
      961 aaggcgaact tcaagatccg ccacaacgtt gagggcggcg gcgtgcagct cgcctaccac
     1021 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1081 agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1141 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1201 ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1261 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gtggcgaggg cgagggcgat
     1321 gccaccaatg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1381 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcgcacg ctaccccgac
     1441 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc
     1501 accatcgttt tcaaggacga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc
     1561 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1621 ctggggcaca agctggagta caaccgggtg aatcctgtgg agctgggccc tggagccttc
     1681 tttggggaga tggccttgat ctctggagaa cccgaggtgg ccactgtctc agctgccacc
     1741 acggtgagcc tgctgtctct gcactccgct gatttccaaa tgctgtgcag ctcttcccca
     1801 gagatcgcag aaatcttcag gaagacagcc ctggagagac gaggagcagc tgcctcagcc
     1861 taagaattcg atatcaagct tatcgatcat caacctctgg attacaaaat ttgtgaaaga
     1921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2281 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     2341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2461 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     2521 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2581 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2641 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     2701 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     2761 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     2821 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     2881 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     2941 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3001 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3061 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3121 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3541 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     3601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5221 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5761 t
//
