LOCUS       Exported               10687 bp ds-DNA     circular SYN 05-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Princeton Network
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 5, 2022 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    550..729
                     /gene="cPPT"
                     /label=cPPT
     promoter        762..1255
                     /gene="pSFFV"
                     /label=SFFV
                     /note="PROMOTER "
     CDS             1331..2278
                     /codon_start=1
                     /gene="irFP"
                     /label=iRFP
                     /note="CDS "
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             2291..3064
                     /codon_start=1
                     /product="TANDEM SH2 DOMAIN FROM ZAP70"
                     /label=tandem SH2
                     /translation="PDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGY
                     VLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRP
                     SGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWY
                     HSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCI
                     PEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSA"
     CDS             2315..2593
                     /codon_start=1
                     /label=SH2 1
                     /translation="FFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDV
                     RFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPC"
     CDS             2774..3049
                     /codon_start=1
                     /label=SH2 2
                     /translation="WYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKT
                     VYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEAC"
     misc_feature    3128..3719
                     /gene="wPRE"
                     /label=WPRE
     gene            4950..5810
                     /gene="AmpR"
                     /label=bla (AmpR)
                     /note="CDS "
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
      781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta
      841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 ggtcgccacc atggctgaag gatccgtcgc caggcagcct gacctcttga cctgcgacga
     1381 tgagccgatc catatccccg gtgccatcca accgcatgga ctgctgctcg ccctcgccgc
     1441 cgacatgacg atcgttgccg gcagcgacaa ccttcccgaa ctcaccggac tggcgatcgg
     1501 cgccctgatc ggccgctctg cggccgatgt cttcgactcg gagacgcaca accgtctgac
     1561 gatcgccttg gccgagcccg gggcggccgt cggagcaccg atcactgtcg gcttcacgat
     1621 gcgaaaggac gcaggcttca tcggctcctg gcatcgccat gatcagctca tcttcctcga
     1681 gctcgagcct ccccagcggg acgtcgccga gccgcaggcg ttcttccgcc gcaccaacag
     1741 cgccatccgc cgcctgcagg ccgccgaaac cttggaaagc gcctgcgccg ccgcggcgca
     1801 agaggtgcgg aagattaccg gcttcgatcg ggtgatgatc tatcgcttcg cctccgactt
     1861 cagcggcgaa gtgatcgcag aggatcggtg cgccgaggtc gagtcaaaac taggcctgca
     1921 ctatcctgcc tcaaccgtgc cggcgcaggc ccgtcggctc tataccatca acccggtacg
     1981 gatcattccc gatatcaatt atcggccggt gccggtcacc ccagacctca atccggtcac
     2041 cgggcggccg attgatctta gcttcgccat cctgcgcagc gtctcgcccg tccatctgga
     2101 attcatgcgc aacataggca tgcacggcac gatgtcgatc tcgattttgc gcggcgagcg
     2161 actgtgggga ttgatcgttt gccatcaccg aacgccgtac tacgtcgatc tcgatggccg
     2221 ccaagcctgc gagctagtcg cccaggttct ggcctggcag atcggcgtga tggaagaggg
     2281 ctccggctct ccagaccccg cggcgcacct gcccttcttc tacggcagca tctcgcgtgc
     2341 cgaggccgag gagcacctga agctggcggg catggcggac gggctcttcc tgctgcgcca
     2401 gtgcctgcgc tcgctgggcg gctatgtgct gtcgctcgtg cacgatgtgc gcttccacca
     2461 ctttcccatc gagcgccagc tcaacggcac ctacgccatt gccggcggca aagcgcactg
     2521 tggaccggca gagctctgcg agttctactc gcgcgacccc gacgggctgc cctgcaacct
     2581 gcgcaagccg tgcaaccggc cgtcgggcct cgagccgcag ccgggggtct tcgactgcct
     2641 gcgagacgcc atggtgcgtg actacgtgcg ccagacgtgg aagctggagg gcgaggccct
     2701 ggagcaggcc atcatcagcc aggccccgca ggtggagaag ctcattgcta cgacggccca
     2761 cgagcggatg ccctggtacc acagcagcct gacgcgtgag gaggccgagc gcaaacttta
     2821 ctctggggcg cagaccgacg gcaagttcct gctgaggccg cggaaggagc agggcacata
     2881 cgccctgtcc ctcatctatg ggaagacggt gtaccactac ctcatcagcc aagacaaggc
     2941 gggcaagtac tgcattcccg agggcaccaa gtttgacacg ctctggcagc tggtggagta
     3001 tctgaagctg aaggcggacg ggctcatcta ctgcctgaag gaggcctgcc ccaacagcag
     3061 tgcctgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     3121 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3181 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3241 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3301 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3361 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3421 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3481 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3541 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3601 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3661 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3721 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3781 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     3841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     4021 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     4081 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     4141 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     4201 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     4261 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     4321 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     4381 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     4441 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     4501 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     4561 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     4621 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4681 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4741 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6721 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6781 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6961 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7021 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7081 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7141 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     7201 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     7261 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     7321 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     7381 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     7441 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     7501 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     7561 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     7621 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     7681 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     7741 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     7801 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     7861 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     7921 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     7981 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     8041 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     8101 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     8161 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     8221 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     8281 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     8341 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     8401 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     8461 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     8521 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     8581 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     8641 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     8701 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     8761 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     8821 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8881 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9061 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9121 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     9181 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
     9241 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     9301 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     9361 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     9421 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     9481 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     9541 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     9601 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     9661 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9841 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9901 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9961 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10021 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10081 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10141 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10201 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10261 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10321 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    10381 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10441 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10501 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10561 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10621 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10681 gtctggg
//
