LOCUS       Mammalian_SaAID_       10384 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..35
                     /label=sy314
                     /note="SY21 dual promoterop"
     primer_bind     complement(3..17)
                     /label=sy204
                     /note="vector Fw"
     primer_bind     complement(106..129)
                     /label=Sy394
     primer_bind     115..129
                     /label=Sy395
     primer_bind     174..193
                     /label=T7 promoter
     primer_bind     209..232
                     /label=al105
                     /note="gRNA vector,50"
     primer_bind     210..232
                     /label=al283
                     /note="AAV-SaCas9-E,52"
     primer_bind     210..232
                     /label=sy228
                     /note="SY4 iii(TM)A@EgEaEiEXE|E]Eiop BamHI"
     primer_bind     211..232
                     /label=kn1302_H1p_MluI_GB_F
     misc_feature    212..440
                     /label=H1 promoter
     primer_bind     212..232
                     /label=Sy525
                     /note="H1eO"
     primer_bind     212..232
                     /label=Sy550
     primer_bind     complement(418..442)
                     /label=al171
                     /note="H1+4_Sp,40"
     primer_bind     complement(421..440)
                     /label=9/25_6
     primer_bind     complement(421..440)
                     /label=sy254
                     /note="H1 promoter Rv"
     primer_bind     complement(424..440)
                     /label=kn1314_HPRT_T1F_R
     primer_bind     426..497
                     /label=9/25_4
     primer_bind     459..536
                     /label=Sy562
     primer_bind     459..485
                     /label=Sy526
                     /note="CaRNA"
     primer_bind     459..481
                     /label=al385
                     /note="Opti_Sa_Scaffold,51"
     misc_RNA        461..536
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     primer_bind     complement(467..536)
                     /label=9/25_5
     primer_bind     492..543
                     /label=al384
                     /note="Opti_Sa_Scaffold,57"
     primer_bind     complement(515..537)
                     /label=Sy562
     primer_bind     complement(519..543)
                     /label=al363
                     /note="oppose-gRNA,37,al363_g,ACCGATGTTCCAATCAGTACGCA,U6_Sa
                      _FANCF"
     primer_bind     complement(519..541)
                     /label=al400
                     /note="LV_C,38"
     primer_bind     complement(521..543)
                     /label=al403
                     /note="Xho,23"
     primer_bind     complement(521..543)
                     /label=al386
                     /note="Opti_Sa_Scaffold,38"
     primer_bind     522..557
                     /label=Sy564
     primer_bind     complement(541..566)
                     /label=Sy551
     primer_bind     543..566
                     /label=Sy395
     primer_bind     543..566
                     /label=Sy332
                     /note="SY4 gRNAi≤C´"
     primer_bind     544..566
                     /label=sy301
                     /note="CMV promoter"
     primer_bind     545..565
                     /label=sy105
                     /note="SY4A@EF1a replace SpeI site"
     primer_bind     546..563
                     /label=al291
     enhancer        551..930
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(551..592)
                     /label=kn1303_Hs-pCMV_start_R
     primer_bind     552..566
                     /label=Sy552
     primer_bind     complement(607..626)
                     /label=kn1312_phCMV100_R
     primer_bind     complement(633..656)
                     /label=sy30
                     /note="Rv1 1906C?UGI in"
     primer_bind     633..656
                     /label=sy31
                     /note="Fw2 1906C?UGI in"
     primer_bind     complement(833..849)
                     /label=sy30
                     /note="Rv1 1906C?UGI in"
     primer_bind     833..849
                     /label=sy31
                     /note="Fw2 1906C?UGI in"
     promoter        931..1134
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     997..1008
                     /label=Sy496
     primer_bind     1031..1049
                     /label=kn1304_pCMVend100_F
     primer_bind     1062..1082
                     /label=Sy496
     primer_bind     1096..1147
                     /label=al338
                     /note="minCMV,62"
     primer_bind     1115..1135
                     /label=al342
                     /note="Scp3,44"
     primer_bind     1150..1174
                     /label=Cas9 Fw
     primer_bind     complement(1151..1174)
                     /label=Sy444
     primer_bind     1151..1174
                     /label=Sy384
     primer_bind     complement(1151..1174)
                     /label=sy316
                     /note="CMV Rv Cas9 vectorop"
     primer_bind     1159..1241
                     /label=sy315
                     /note="Cas9 Fw mitchy's vector"
     primer_bind     1179..1198
                     /label=T7 promoter
     promoter        1179..1197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1194..1227)
                     /label=Sy426
     misc_feature    1217..1219
                     /label=kozac
     CDS             1220..1270
                     /codon_start=1
                     /label=NLS unit
                     /translation="MAPKKKRKVGIHGVPAA"
     primer_bind     1220..1241
                     /label=Sy425
     CDS             1226..1246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1226..1246)
                     /label=Sy444
     primer_bind     complement(1247..1291)
                     /label=al102
                     /note="SaCas9 vector,45"
     primer_bind     complement(1254..1270)
                     /label=sy256
                     /note="NLS Rv"
     primer_bind     1255..1271
                     /label=sy257
                     /note="ugi Fw+overlap"
     primer_bind     1256..1290
                     /label=Sy565
     primer_bind     1256..1271
                     /label=sy263
                     /note="AID"
     primer_bind     1256..1271
                     /label=sy272
     primer_bind     1260..1294
                     /label=al103
                     /note="SaCas9 vector,35"
     CDS             1271..4426
                     /codon_start=1
                     /label=nSaCas9
                     /translation="KRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     primer_bind     1271..1294
                     /label=al294
                     /note="AAV-AN15GS,57"
     primer_bind     1271..1290
                     /label=9/25_2
     primer_bind     1271..1290
                     /label=Sy568
     CDS             1295..1297
                     /codon_start=1
                     /label=D10A
                     /translation="A"
     primer_bind     complement(1630..1651)
                     /label=al109
                     /note="""""SaCas9 vector127,128"""",50"
     primer_bind     1636..1666
                     /label=al110
                     /note="""""SaCas9 vector127,128"""",70"
     primer_bind     1885..1904
                     /label=Sy569
     misc_feature    1925
                     /label=?????? 27
     primer_bind     2265..2281
                     /label=al136
                     /note="dCas9/dCas9-UGI-UGI,17"
     primer_bind     complement(2378..2402)
                     /label=al295
                     /note="AAV-AN15GS,25"
     primer_bind     2597..2616
                     /label=Sy570
     misc_feature    2654
                     /label=?????? 28
     primer_bind     complement(2655..2676)
                     /label=al288
                     /note="nSaCas9,22"
     primer_bind     complement(2677..2713)
                     /label=al166
                     /note="pAL062(L1 half Tru),37"
     primer_bind     2689..2778
                     /label=al167
                     /note="pAL062(L1 half Tru),45"
     primer_bind     complement(2696..2713)
                     /label=al168
                     /note="pAL063(HNH Tru),45"
     primer_bind     complement(2731..2755)
                     /label=al241
                     /note="pAL066_1r,50"
     primer_bind     complement(2805..2824)
                     /label=al239
                     /note="pAL065_1r,45"
     primer_bind     complement(2805..2824)
                     /label=al234
                     /note="pAL064_1r,45"
     primer_bind     complement(2843..2863)
                     /label=al162
                     /note="pAL060(L1 linker Rigid) ,41"
     primer_bind     complement(2866..2884)
                     /label=al112
                     /note="""""SaCas9 vector538,539"""",40"
     primer_bind     2885..2903
                     /label=al163
                     /note="pAL060(L1 linker Rigid) ,45"
     primer_bind     2885..2903
                     /label=al113
                     /note="""""SaCas9 vector538,539"""",70"
     primer_bind     complement(2984..3018)
                     /label=al137
                     /note="dCas9/dCas9-UGI-UGI,35"
     primer_bind     2990..3024
                     /label=al138
                     /note="dCas9/dCas9-UGI-UGI,35"
     primer_bind     complement(3082..3112)
                     /label=al114
                     /note="""""SaCas9 vector614,615"""",50"
     primer_bind     complement(3082..3112)
                     /label=al160
                     /note="pAL058 (pAL052 PAPAPAP-Nter linker),56"
     primer_bind     3112..3146
                     /label=al157
                     /note="pAL056 (pAL052 without GS linker),59"
     primer_bind     3113..3146
                     /label=al159
                     /note="pAL057 (pAL052 PAPAPAP-Cter linker),55"
     primer_bind     3113..3133
                     /label=al115
                     /note="""""SaCas9 vector614,615"""",70"
     primer_bind     complement(3123..3148)
                     /label=al164
                     /note="pAL061(L2 linker Rigid) ,45"
     primer_bind     3160..3190
                     /label=al240
                     /note="pAL065_3f,55"
     primer_bind     3161..3190
                     /label=al235
                     /note="pAL064_3f,55"
     primer_bind     3167..3190
                     /label=al165
                     /note="pAL061(L2 linker Rigid) ,49"
     primer_bind     3213..3238
                     /label=al169
                     /note="pAL063(HNH Tru),49"
     primer_bind     complement(3282..3302)
                     /label=?????? 2
     primer_bind     3282..3302
                     /label=?????? 1
     primer_bind     3315..3334
                     /label=Sy571
     primer_bind     complement(3317..3340)
                     /label=al116
                     /note="""""SaCas9 vector690,691"""",55"
     primer_bind     3341..3356
                     /label=al117
                     /note="""""SaCas9 vector690,691"""",70"
     misc_feature    3366
                     /label=?????? 29
     primer_bind     complement(3458..3475)
                     /label=al118
                     /note="""""SaCas9 vector735,736"""",50"
     primer_bind     3476..3491
                     /label=al119
                     /note="""""SaCas9 vector735,736"""",70"
     primer_bind     complement(3521..3545)
                     /label=al236
                     /note="pAL064_3r,25"
     primer_bind     complement(3796..3814)
                     /label=al120
                     /note="""""SaCas9 vector848,849"""",50"
     primer_bind     3814..3829
                     /label=al121
                     /note="""""SaCas9 vector848,849"""",70"
     primer_bind     3966..3982
                     /label=al132
                     /note="nCas9-UGI-UGI,17"
     primer_bind     complement(4031..4050)
                     /label=al111
                     /note="SaCas9 vector,20"
     primer_bind     4057..4076
                     /label=Sy572
     misc_feature    4085
                     /label=?????? 30
     primer_bind     4301..4312
                     /label=kn892_yCas9XmaKO2_F
     primer_bind     complement(4407..4426)
                     /label=Sy566
     primer_bind     4412..4446
                     /label=Sy567
     primer_bind     4412..4437
                     /label=9/25_01
     primer_bind     4423..4449
                     /label=kn1085_ApaNLS_F
     primer_bind     complement(4427..4450)
                     /label=al092
                     /note="Cas-UGI,35"
     CDS             4427..4447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4427..4447)
                     /label=al004
                     /note="dCas9_XbaI,45"
     primer_bind     complement(4427..4447)
                     /label=al256
                     /note="Yeast_nCas9,54"
     primer_bind     complement(4427..4447)
                     /label=kn946_Cas9-nosT_R
     primer_bind     4427..4446
                     /label=kn588_Flag-NLS_F
     primer_bind     complement(4428..4447)
                     /label=Sy424
                     /note="GS linker SY20"
     primer_bind     complement(4428..4447)
                     /label=Sy421
                     /note="GS linker SY20"
     primer_bind     complement(4428..4447)
                     /label=Sy389
     primer_bind     complement(4428..4447)
                     /label=sy317
                     /note="Cas9 Rv mitchys vector"
     primer_bind     complement(4428..4447)
                     /label=Sy473
     primer_bind     4432..4450
                     /label=al093
                     /note="Cas-UGI,32"
     primer_bind     4433..4447
                     /label=An01
     primer_bind     complement(4434..4453)
                     /label=sy268
                     /note="nCas9 Rv2"
     primer_bind     4439..4455
                     /label=sy269
     CDS             4478..4648
                     /codon_start=1
                     /label=dead SH3 hs
                     /translation="AEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGK
                     RGMILVPYVEKY"
     primer_bind     4478..4499
                     /label=Sy344
     primer_bind     4478..4499
                     /label=sy265
     primer_bind     4589..4612
                     /label=Sy391
     primer_bind     complement(4629..4654)
                     /label=sy266
     primer_bind     4640..4654
                     /label=Sy568
     primer_bind     complement(4649..4666)
                     /label=sy181
                     /note="at002_3G"
     CDS             4655..4720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG hs
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     4687..4715
                     /label=sy20
                     /note="R9+GS linker (Cnn) Rv"
     primer_bind     complement(4702..4719)
                     /label=kn578_Flag-Cas9_R
     primer_bind     4726..4747
                     /label=Sy422
                     /note="GS linker SY20"
     CDS             4727..5350
                     /codon_start=1
                     /label=HsPmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     primer_bind     4727..4754
                     /label=al389
                     /note="In_Sy26_BE4max,28"
     primer_bind     4727..4747
                     /label=Sy461
     primer_bind     4727..4747
                     /label=Sy456
     primer_bind     4727..4747
                     /label=Sy423
                     /note="GS linker SY20"
     primer_bind     4727..4747
                     /label=kn680_PmCDA1_start_F
     primer_bind     4727..4747
                     /label=sy263
                     /note="AID"
     primer_bind     4727..4747
                     /label=sy270
     primer_bind     4727..4747
                     /label=Sy524
                     /note="CDA1"
     primer_bind     4728..4747
                     /label=An01
     primer_bind     complement(4813..4845)
                     /label=al118
                     /note="""""SaCas9 vector735,736"""",50"
     primer_bind     complement(4813..4844)
                     /label=al116
                     /note="""""SaCas9 vector690,691"""",55"
     primer_bind     complement(4813..4840)
                     /label=al168
                     /note="pAL063(HNH Tru),45"
     primer_bind     complement(4813..4834)
                     /label=al112
                     /note="""""SaCas9 vector538,539"""",40"
     primer_bind     complement(4813..4832)
                     /label=al114
                     /note="""""SaCas9 vector614,615"""",50"
     primer_bind     complement(4814..4844)
                     /label=al120
                     /note="""""SaCas9 vector848,849"""",50"
     primer_bind     complement(4814..4841)
                     /label=al109
                     /note="""""SaCas9 vector127,128"""",50"
     primer_bind     complement(4814..4838)
                     /label=al234
                     /note="pAL064_1r,45"
     primer_bind     4814..4832
                     /label=al107
                     /note="CDA1,19"
     primer_bind     4814..4832
                     /label=al242
                     /note="pAL067_2f,28"
     primer_bind     4814..4832
                     /label=al237
                     /note="pAL065_2f,28"
     primer_bind     4814..4832
                     /label=al161
                     /note="pAL058 (pAL052 PAPAPAP-Nter linker),40"
     primer_bind     complement(4814..4829)
                     /label=al241
                     /note="pAL066_1r,50"
     primer_bind     complement(4814..4829)
                     /label=al239
                     /note="pAL065_1r,45"
     primer_bind     5053..5077
                     /label=al407
                     /note="NLS_N,25"
     primer_bind     complement(5122..5176)
                     /label=al108
                     /note="CDA1,55"
     primer_bind     complement(5151..5177)
                     /label=al248
                     /note="pAL069_2r,44"
     primer_bind     complement(5151..5176)
                     /label=al232
                     /note="AID2S-3ter,26"
     primer_bind     complement(5151..5176)
                     /label=al246
                     /note="pAL068_2r,35"
     primer_bind     complement(5151..5176)
                     /label=al238
                     /note="pAL065_2r,35"
     primer_bind     complement(5151..5176)
                     /label=al293
                     /note="AAV-AN15GS,59"
     primer_bind     5152..5186
                     /label=sy306
     primer_bind     5152..5178
                     /label=al235
                     /note="pAL064_3f,55"
     primer_bind     5152..5176
                     /label=al157
                     /note="pAL056 (pAL052 without GS linker),59"
     primer_bind     5155..5176
                     /label=al110
                     /note="""""SaCas9 vector127,128"""",70"
     primer_bind     5155..5176
                     /label=al121
                     /note="""""SaCas9 vector848,849"""",70"
     primer_bind     complement(5155..5176)
                     /label=al158
                     /note="pAL057 (pAL052 PAPAPAP-Cter linker),50"
     primer_bind     5156..5176
                     /label=al117
                     /note="""""SaCas9 vector690,691"""",70"
     primer_bind     5156..5176
                     /label=al119
                     /note="""""SaCas9 vector735,736"""",70"
     primer_bind     5159..5176
                     /label=al113
                     /note="""""SaCas9 vector538,539"""",70"
     primer_bind     5161..5176
                     /label=al115
                     /note="""""SaCas9 vector614,615"""",70"
     primer_bind     5161..5176
                     /label=al240
                     /note="pAL065_3f,55"
     primer_bind     complement(5275..5296)
                     /label=sy305
     primer_bind     5275..5296
                     /label=sy304
     primer_bind     complement(5329..5350)
                     /label=Sy385
     primer_bind     complement(5329..5350)
                     /label=sy264
     primer_bind     5330..5398
                     /label=al097
                     /note="PmCDA1-UGI,60"
     primer_bind     5336..5351
                     /label=sy265
     primer_bind     5336..5350
                     /label=9/25_2
     primer_bind     5350..5375
                     /label=Sy343
     primer_bind     5351..5375
                     /label=sy269
     primer_bind     5351..5364
                     /label=9/25_01
     primer_bind     complement(5352..5380)
                     /label=sy32
                     /note="Rv2 1906C?UGI in"
     primer_bind     complement(5354..5388)
                     /label=al092
                     /note="Cas-UGI,35"
     CDS             5354..5374
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     5359..5390
                     /label=al093
                     /note="Cas-UGI,32"
     primer_bind     complement(5359..5380)
                     /label=Sy445
     primer_bind     complement(5359..5380)
                     /label=sy260
                     /note="AID Rv"
     primer_bind     5365..5380
                     /label=Sy446
     primer_bind     5380..5401
                     /label=sy180
                     /note="at001_3G"
     CDS             5381..5632
                     /codon_start=1
                     /label=Ugi
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     primer_bind     5381..5401
                     /label=sy257
                     /note="ugi Fw+overlap"
     primer_bind     5381..5401
                     /label=sy272
     primer_bind     5381..5395
                     /label=kn1430_Gal10-Ugi_F
     primer_bind     complement(5608..5635)
                     /label=sy309
                     /note="SY20 dual promoterop"
     primer_bind     complement(5608..5633)
                     /label=sy273
     primer_bind     complement(5608..5632)
                     /label=sy258
                     /note="ugi Rv"
     primer_bind     complement(5617..5635)
                     /label=al094
                     /note="Cas-UGI,50"
     primer_bind     5620..5653
                     /label=sy308
                     /note="SY20 dual promoterop"
     primer_bind     complement(5621..5635)
                     /label=Sy556
     primer_bind     complement(5621..5635)
                     /label=Sy529
     primer_bind     5633..5659
                     /label=sy47
                     /note="Fw SV40 polyA,60"
     primer_bind     5633..5653
                     /label=Sy390
     primer_bind     5633..5653
                     /label=Sy386
     primer_bind     5633..5653
                     /label=sy159
                     /note="SY6 iZC="
     primer_bind     5636..5653
                     /label=Sy446
     polyA_signal    5649..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5760..5781)
                     /label=sy48
                     /note="Rv SV40 polyA,59"
     primer_bind     complement(5808..5847)
                     /label=sy255
                     /note="Rv+overlap"
     primer_bind     complement(5808..5831)
                     /label=Sy449
     primer_bind     5831..5855
                     /label=Sy552
     primer_bind     5832..5855
                     /label=sy238
                     /note="SY5 short"
     primer_bind     complement(5832..5847)
                     /label=sy239
                     /note="SY5 short"
     primer_bind     complement(5832..5846)
                     /label=sy242
                     /note="SY6"
     misc_feature    5876..6464
                     /label=ori
     primer_bind     6154..6179
                     /label=sy208
                     /note="Amp_ori_Rv"
     primer_bind     complement(6492..6513)
                     /label=sy108
                     /note="1935#1 for IRESmEGFP Rv,59"
     primer_bind     6492..6513
                     /label=sy109
                     /note="1935#2 for IRESmEGFP Fw,59"
     exon            6638..7495
                     /label=Amp R
     primer_bind     complement(6718..6739)
                     /label=sy207
                     /note="Amp_ori_Fw"
     misc_feature    7496..7600
                     /label=AmpR promoter
     primer_bind     7572..7600
                     /label=al105
                     /note="gRNA vector,50"
     primer_bind     complement(7574..7600)
                     /label=al106
                     /note="gRNA vector,27"
     primer_bind     complement(7584..7600)
                     /label=al100
                     /note="SaCas9 vector,45"
     primer_bind     7930..7953
                     /label=Sy427
                     /note="For EF1a seq"
     primer_bind     complement(7930..7953)
                     /label=sy302
     primer_bind     7939..7977
                     /label=sy310
                     /note="SY20 dual promoteropA@EF1aeuiuA@OL with 302"
     primer_bind     7939..7953
                     /label=sy301
                     /note="CMV promoter"
     primer_bind     7954..7977
                     /label=sy293
                     /note="EF1a_SpeI_Fw"
     primer_bind     7954..7977
                     /label=sy274
     primer_bind     7954..7977
                     /label=sy130
                     /note="EF1a GA with SY1-3 Fw"
     primer_bind     7954..7977
                     /label=sy105
                     /note="SY4A@EF1a replace SpeI site"
     primer_bind     7954..7977
                     /label=sy102
                     /note="EF1a Fw + overlap,59"
     promoter        7995..9172
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     primer_bind     8013..8037
                     /label=al345
                     /note="core EF-1a,41"
     primer_bind     8013..8030
                     /label=Sy522
                     /note="Ef1a"
     primer_bind     complement(8206..8225)
                     /label=al346
                     /note="core EF-1a,50"
     intron          8226..9163
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     primer_bind     9120..9140
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(9172..9194)
                     /label=sy275
     primer_bind     complement(9172..9193)
                     /label=sy294
                     /note="EF1a_EcoRI_Rv"
     primer_bind     complement(9172..9192)
                     /label=sy131
                     /note="EF1a GA with SY1-3 Rv"
     primer_bind     complement(9172..9192)
                     /label=sy106
                     /note="SY4A@EF1a replace NheI site"
     primer_bind     complement(9172..9192)
                     /label=sy103
                     /note="EF1a Rv,59"
     primer_bind     9178..9216
                     /label=sy243
                     /note="iRFP Fw with overlap for SY6"
     primer_bind     9179..9216
                     /label=sy311
                     /note="SY20 dual promoteropA@iRFP kozak OL with 103"
     CDS             9199..10134
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     primer_bind     9199..9216
                     /label=sy240
                     /note="iRFP Fw with overlap"
     regulatory      9199..9202
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     9202..9220
                     /label=Sy450
     primer_bind     complement(9365..9386)
                     /label=Sy383
     primer_bind     9365..9386
                     /label=Sy382
     primer_bind     10105..10129
                     /label=al233
                     /note="pAL064_1f,25"
     primer_bind     complement(10131..10155)
                     /label=sy292
     primer_bind     complement(10131..10149)
                     /label=sy241
                     /note="IRFP Rv"
     primer_bind     complement(10131..10149)
                     /label=sy244
                     /note="iRFP Rv with overlap for SY6"
     primer_bind     complement(10131..10149)
                     /label=sy251
                     /note="SY20 Rv iRFPeuiu"
     primer_bind     10141..10175
                     /label=sy312
                     /note="SY20 dual promoterop BGH polyA OL292"
     primer_bind     10156..10175
                     /label=sy231
                     /note="SY4 iii(TM)A@EgEaEiEXE|E]Eiop BamHI Rv2"
     primer_bind     10156..10175
                     /label=sy229
                     /note="SY4 iii(TM)A@EgEaEiEXE|E]Eiop EcoRI"
     primer_bind     10156..10175
                     /label=sy239
                     /note="SY5 short"
     primer_bind     10156..10172
                     /label=sy233
                     /note="1929 EGFP Fw with BamHI"
     primer_bind     10156..10168
                     /label=sy235
                     /note="SY15-3 RFP Rv BamHI"
     primer_bind     10156..10167
                     /label=sy242
                     /note="SY6"
     polyA_signal    join(10164..10384,1..4)
                     /label=bGH poly(A)
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10164..10183
                     /label=al134
                     /note="nCas9/nCas9-UGI-UGI,50"
     primer_bind     complement(join(10373..10384,1..4))
                     /label=al135
                     /note="nCas9,36"
     primer_bind     complement(join(10380..10384,1..15))
                     /label=sy313
                     /note="SY20 dual promoterop BGH polyA"
     primer_bind     complement(join(10380..10384,1..15))
                     /label=Sy478
     primer_bind     complement(join(10380..10384,1..15))
                     /label=Sy483
ORIGIN
        1 acagtcgagg ctgatcattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
      181 actcactata gggcgaattg ggtaccgggc caattcgaac gctgacgtca tcaacccgct
      241 ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc
      301 aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt
      361 gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt ataagttctg
      421 tatgaggacc acagatcccc gatgttccaa tcagtacgca gttttagtac tctggaaaca
      481 gaatctacta aaacaaggca aaatgccgtg tttatctcgt caacttgttg gcgagatttt
      541 tttacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      601 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      661 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      721 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      781 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      841 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      901 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      961 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     1021 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1081 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     1141 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     1201 acccaagctg gctagcgaaa tggcaccgaa gaagaagcgt aaagtcggaa tccacggagt
     1261 tcctgcggca aagcgaaatt acattctggg gctggccatt ggcattacat cagtgggcta
     1321 tggcatcatt gactacgaga caagggacgt gatcgacgcc ggcgtgagac tgttcaagga
     1381 ggccaacgtg gagaacaatg agggccggag atccaagagg ggagcaaggc gcctgaagcg
     1441 gagaaggcgc cacagaatcc agagagtgaa gaagctgctg ttcgattaca acctgctgac
     1501 cgaccactcc gagctgtctg gcatcaatcc ttatgaggcc agagtgaagg gcctgtccca
     1561 gaagctgtct gaggaggagt ttagcgccgc cctgctgcac ctggcaaaga ggagaggcgt
     1621 gcacaacgtg aatgaggtgg aggaggacac cggcaacgag ctgtccacaa aggagcagat
     1681 cagccgcaat tccaaggccc tggaggagaa gtatgtggcc gagctgcagc tggagcggct
     1741 gaagaaggat ggcgaggtga ggggctccat caatcgcttc aagacctctg actacgtgaa
     1801 ggaggccaag cagctgctga aggtgcagaa ggcctaccac cagctggatc agtcctttat
     1861 cgatacatat atcgacctgc tggagacaag gcgcacatac tatgagggac caggagaggg
     1921 ctctcccttc ggctggaagg acatcaagga gtggtacgag atgctgatgg gccactgcac
     1981 ctattttcca gaggagctga gaagcgtgaa gtacgcctat aacgccgatc tgtacaacgc
     2041 cctgaatgac ctgaacaacc tggtcatcac cagggatgag aacgagaagc tggagtacta
     2101 tgagaagttc cagatcatcg agaacgtgtt caagcagaag aagaagccta cactgaagca
     2161 gatcgccaag gagatcctgg tgaacgagga ggacatcaag ggctaccgcg tgacctccac
     2221 aggcaagcca gagttcacca atctgaaggt gtatcacgat atcaaggaca tcacagcccg
     2281 gaaggagatc atcgagaacg ccgagctgct ggatcagatc gccaagatcc tgaccatcta
     2341 tcagagctcc gaggacatcc aggaggagct gaccaacctg aatagcgagc tgacacagga
     2401 ggagatcgag cagatcagca atctgaaggg ctacaccggc acacacaacc tgagcctgaa
     2461 ggccatcaat ctgatcctgg atgagctgtg gcacacaaac gacaatcaga tcgccatctt
     2521 taaccggctg aagctggtgc caaagaaggt ggacctgtcc cagcagaagg agatcccaac
     2581 cacactggtg gacgatttca tcctgtctcc cgtggtgaag cggagcttca tccagagcat
     2641 caaagtgatc aacgccatca tcaagaagta cggcctgccc aatgatatca tcatcgagct
     2701 ggccagggag aagaactcca aggacgccca gaagatgatc aatgagatgc agaagaggaa
     2761 ccgccagacc aatgagcgga tcgaggagat catcagaacc acaggcaagg agaacgccaa
     2821 gtacctgatc gagaagatca agctgcacga tatgcaggag ggcaagtgtc tgtattctct
     2881 ggaggccatc cctctggagg acctgctgaa caatccattc aactacgagg tggatcacat
     2941 catcccccgg agcgtgagct tcgacaattc ttttaacaat aaggtgctgg tgaagcagga
     3001 ggagaacagc aagaagggca ataggacccc tttccagtac ctgtctagct ccgattctaa
     3061 gatcagctac gagacattca agaagcacat cctgaatctg gccaagggca agggccgcat
     3121 cagcaagacc aagaaggagt acctgctgga ggagcgggac atcaacagat tctccgtgca
     3181 gaaggacttc atcaaccgga atctggtgga caccagatac gccacacgcg gcctgatgaa
     3241 tctgctgcgg tcttatttca gagtgaacaa tctggatgtg aaggtgaaga gcatcaacgg
     3301 cggcttcacc tcctttctgc ggagaaagtg gaagtttaag aaggagcgca acaagggcta
     3361 taagcaccac gccgaggatg ccctgatcat cgccaatgcc gacttcatct ttaaggagtg
     3421 gaagaagctg gacaaggcca agaaagtgat ggagaaccag atgttcgagg agaagcaggc
     3481 cgagagcatg cccgagatcg agacagagca ggagtacaag gagattttca tcacacctca
     3541 ccagatcaag cacatcaagg acttcaagga ctacaagtat tctcacaggg tggataagaa
     3601 gcccaaccgc gagctgatca atgacaccct gtatagcaca cggaaggacg ataagggcaa
     3661 taccctgatc gtgaacaatc tgaacggcct gtacgacaag gataatgaca agctgaagaa
     3721 gctgatcaac aagtctcccg agaagctgct gatgtaccac cacgatcctc agacatatca
     3781 gaagctgaag ctgatcatgg agcagtacgg cgacgagaag aacccactgt ataagtacta
     3841 tgaggagaca ggcaactacc tgacaaagta tagcaagaag gataatggcc ccgtgatcaa
     3901 gaagatcaag tactatggca acaagctgaa tgcccacctg gacatcaccg acgattaccc
     3961 taactctcgc aataaggtgg tgaagctgag cctgaagcca taccggttcg acgtgtacct
     4021 ggacaacggc gtgtataagt ttgtgacagt gaagaatctg gatgtgatca agaaggagaa
     4081 ctactatgag gtgaacagca agtgctacga ggaggccaag aagctgaaga agatcagcaa
     4141 ccaggccgag ttcatcgcct ctttttacaa caatgacctg atcaagatca atggcgagct
     4201 gtatagagtg atcggcgtga acaatgatct gctgaacaga atcgaagtga atatgatcga
     4261 catcacctac agggagtatc tggagaacat gaatgataag aggccccctc gcatcatcaa
     4321 gaccatcgcc tctaagacac agagcatcaa gaagtacagc acagacatcc tggggaacct
     4381 gtatgaagtc aagagcaaga aacatcctca gattatcaag aaaggcccca agaagaagag
     4441 gaaggtgggt ggaggaggta ccggcggtgg aggctcagca gaatacgtac gagctctgtt
     4501 tgacttcaat gggaatgacg aggaggatct cccctttaag aagggcgata ttctccgcat
     4561 cagagataag cccgaagaac aatggtggaa tgccgaggat agcgaaggga aaaggggcat
     4621 gattctggtg ccatatgtgg agaaatattc cggtgactac aaagaccatg atggggatta
     4681 caaagaccac gacatcgact acaaagacga cgacgataaa tcagggatga cagacgccga
     4741 gtacgtgcgc attcatgaga aactggatat ttacaccttc aagaagcagt tcttcaacaa
     4801 caagaaatct gtgtcacacc gctgctacgt gctgtttgag ttgaagcgaa ggggcgaaag
     4861 aagggcttgc ttttggggct atgccgtcaa caagccccaa agtggcaccg agagaggaat
     4921 acacgctgag atattcagta tccgaaaggt ggaagagtat cttcgggata atcctgggca
     4981 gtttacgatc aactggtatt ccagctggag tccttgcgct gattgtgccg agaaaattct
     5041 ggaatggtat aatcaggaac ttcggggaaa cgggcacaca ttgaaaatct gggcctgcaa
     5101 gctgtactac gagaagaatg cccggaacca gataggactc tggaatctga gggacaatgg
     5161 tgtaggcctg aacgtgatgg tttccgagca ctatcagtgt tgtcggaaga ttttcatcca
     5221 aagctctcat aaccagctca atgaaaaccg ctggttggag aaaacactga aacgtgcgga
     5281 gaagcggaga tccgagctga gcatcatgat ccaggtcaag attctgcata ccactaagtc
     5341 tccagccgtt ggtcccaaga agaaaagaaa agtcggtacc atgaccaacc tttccgacat
     5401 catagagaag gaaacaggca aacagttggt catccaagag tcgatactca tgcttcctga
     5461 agaagttgag gaggtcattg ggaataagcc ggaaagtgac attctcgtac acactgcgta
     5521 tgatgagagc accgatgaga acgtgatgct gctcacgtca gatgccccag agtacaaacc
     5581 ctgggctctg gtgattcagg actctaatgg agagaacaag atcaagatgc tatagcttcg
     5641 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5761 atgtatctta gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5821 taattgcgcg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5881 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5941 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6001 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6061 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6301 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6541 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6601 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6661 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6721 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6781 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6841 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6901 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6961 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7021 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7081 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7141 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7201 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7261 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7321 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7381 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7441 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7501 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7561 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7621 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7681 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7741 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7801 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7861 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7921 gagcccccga tttagagctt gacggggaaa gccgcagcta atggaccttc taggtcttga
     7981 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     8041 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     8101 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     8161 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     8221 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     8281 gcgtgccttg aattacttcc actggctgca gtacgtgatt cttgatcccg agcttcgggt
     8341 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     8401 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     8461 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     8521 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     8581 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     8641 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     8701 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     8761 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     8821 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     8881 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     8941 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     9001 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     9061 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     9121 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaatttc
     9181 gacccggatc cggccaccat ggcgcgtaag gtcgatctca cctcctgcga tcgcgagccg
     9241 atccacatcc ccggcagcat tcagccgtgc ggctgcctgc tagcctgcga cgcgcaggcg
     9301 gtgcggatca cgcgcattac ggaaaatgcc ggcgcgttct ttggacgcga aactccgcgg
     9361 gtcggtgagc tactcgccga ttacttcggc gagaccgaag cccatgcgct gcgcaacgca
     9421 ctggcgcagt cctccgatcc aaagcgaccg gcgctgatct tcggttggcg cgacggcctg
     9481 accggccgca ccttcgacat ctcactgcat cgccatgacg gtacatcgat catcgagttc
     9541 gagcctgcgg cggccgaaca ggccgacaat ccgctgcggc tgacgcggca gatcatcgcg
     9601 cgcaccaaag aactgaagtc gctcgaagag atggccgcac gggtgccgcg ctatctgcag
     9661 gcgatgctcg gctatcaccg cgtgatgttg taccgcttcg cggacgacgg ctccgggatg
     9721 gtgatcggcg aggcgaagcg cagcgacctc gagagctttc tcggtcagca ctttccggcg
     9781 tcgctggtcc cgcagcaggc gcggctactg tacttgaaga acgcgatccg cgtggtctcg
     9841 gattcgcgcg gcatcagcag ccggatcgtg cccgagcacg acgcctccgg cgccgcgctc
     9901 gatctgtcgt tcgcgcacct gcgcagcatc tcgccctgcc atctcgaatt tctgcggaac
     9961 atgggcgtca gcgcctcgat gtcgctgtcg atcatcattg acggcacgct atggggattg
    10021 atcatctgtc atcattacga gccgcgtgcc gtgccgatgg cgcagcgcgt cgcggccgaa
    10081 atgttcgccg acttcttatc gctgcacttc accgccgccc accaccaacg ctaagcggcc
    10141 gcgactctaa ccggtctcag aagccataga gcccaccgca tccccagcat gcctgctatt
    10201 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
    10261 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
    10321 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
    10381 aggc
//
