LOCUS       Exported               12044 bp ds-DNA     circular SYN 07-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2151 pHAGE-hSPC-TFEB-TagBFP-W
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   OLIVIA HIX
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2022 from SnapGene 2.7.3
            http://www.snapgene.com
COMMENT     9/10/19 Olivia Hix cloned pHAGE-hSPC-TFEB-TagBFP-W as follows: 
            |SpeI/NotI was used to clone out hSPC promoter and TFEB from 
            pHAGE-hSPC-TFEBeGFP-W and clone into backbone of pHAGE-EF1aL-eGFP-W 
            cut SpeI/NotI. Products were run on a 0.5% agarose gel. The backbone
            and TFEB insert were both, separately, gel extracted. These products
            were ligated together at a 1:2 backbone:insert ratio. Clone 5 was 
            sequenced, which confirmed proper base pairs at the 5' and 3' 
            junctions.|
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..636
                     /gene="LTR"
                     /note="5' LTR"
                     /note="Long Terminal Repeat"
     misc_feature    1..454
                     /note="HIV U3"
                     /note="Full Length HIV U3"
     primer_bind     complement(158..179)
                     /note="pHAGE-A0050-8050"
     primer_bind     401..421
                     /note="pHAGE-S501-1000"
     misc_feature    455..551
                     /note="HIV R"
                     /note="HIV Repeat Region"
     polyA_site      550
                     /note="pA Site"
                     /note="Exact base to which Poly-A is added"
     5'UTR           551..635
                     /note="HIV 5' UTR"
     misc_feature    552..636
                     /note="HIV U5"
     polyA_signal    570..577
                     /note="G-T cluster"
                     /note="GT Cluster shown to be necessary for Poly-A site 
                     activity by Cullen in J Virol. 1989 January; 63(1):421-424"
     misc_feature    636..658
                     /note="PBS-K"
                     /note="tRNA binding site for Lysine tRNA"
     misc_feature    637..1155
                     /gene="psi"
                     /product="pbs-gag"
                     /note="PSI"
                     /note="PSI Packaging Sequence"
     primer_bind     complement(667..690)
                     /note="pHAGE-A0550-0050"
     misc_feature    697..806
                     /note="HIV-PSI"
     stem_loop       697..731
                     /note="SL-1"
                     /note="PSI Stem loop 1"
     stem_loop       736..754
                     /note="SL-2"
                     /note="PSI Stem loop 2"
     misc_feature    744..745
                     /note="5' SD 289"
                     /note="Major 5' Splice Donor 289"
     stem_loop       766..779
                     /note="SL-3"
                     /note="PSI Stem Loop 3"
     CDS             790..1144
                     /note="HIV gag"
                     /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII
                     site in HIV and filled in ClaI digest to take gag CDS out 
                     of frame)"
     stem_loop       793..806
                     /note="SL-4"
                     /note="PSI Stem Loop 4"
     misc_feature    807..1144
                     /note="HR-ePSI"
                     /note="HIV gag sequence 351bp to 688bp"
     misc_feature    833..834
                     /note="Fill-in"
                     /note="Filled in Cla Site from original HIV genome to 
                     disrupt gag polyprotein ORF"
     misc_feature    853..855
                     /note="Stop Codon"
                     /note="Stop codon in gag put in frame by ClaI fill in"
     primer_bind     898..919
                     /note="pHAGE-S1001-1500"
     CDS             1156..2013
                     /note="env"
                     /note="HIV env sequence from bp7168 to 8025 (contains RRE) 
                     Cloned from BglII to BamHI out of HIV"
     primer_bind     complement(1166..1189)
                     /note="pHAGE-A1050-0550"
     misc_feature    1245..1593
                     /note="RRE"
                     /note="Rev Responsive Element according to Belasco in 
                     Molecular Cell, Vol. 7, 603-614, March, 2001"
     misc_feature    1303..1536
                     /gene="rre"
                     /product="minimal RRE"
                     /note="RRE"
                     /note="Rev Responsive Element"
     misc_feature    1357
                     /note="RRE Mutation"
                     /note="This G is a C in the WT HIV-1 genome"
     primer_bind     1391..1417
                     /note="pHAGE-S1501-2000"
     primer_bind     complement(1650..1678)
                     /note="pHAGE-A1550-1050"
     primer_bind     1898..1922
                     /note="pHAGE-S2001-2500"
     misc_feature    1911..1912
                     /note="SA 7925"
     misc_feature    2014..2027
                     /note="Junk"
                     /note="Linker Junk Sequence probably left over from 
                     cloning"
     misc_feature    2034..2211
                     /gene="cppt"
                     /note="HIV cpPu (Trip)"
                     /note="Central Poly Purine Track"
     primer_bind     complement(2084..2112)
                     /note="pHAGE-A2050-1550"
     misc_feature    2188..2189
                     /note="SA 4459"
     misc_feature    2218..2430
                     /note="IVS"
                     /note="intervening sequence from Whitsett hSPC plasmid"
     misc_feature    2218..2430
                     /note="IVS"
                     /note="intervening sequence from Whitsett hSPCp plasmid"
     misc_feature    2431..6244
                     /note="hSP-C promoter"
     misc_difference 2760
                     /note="variation"
                     /note="A in pubmed sequence"
     regulatory      6303..6312
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6309..7736
                     /codon_start=1
                     /note="TFEB"
                     /translation="MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQL
                     GGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKF
                     AAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDN
                     IMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKREL
                     TDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKAS
                     VDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAE
                     LAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHS
                     LSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEA
                     SKASSRRSSFSMEEGDVL"
     misc_feature    6309..6315
                     /note="TFEB START"
     misc_feature    7730..7736
                     /note="END OF TFEB"
     CDS             7776..8477
                     /codon_start=1
                     /note="TagBFP"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8485..9076
                     /gene="WPRE"
                     /note="WPRE"
                     /note="Woodchuck Hepatitis Virus Posttranscriptional 
                     Regulatory Element"
     primer_bind     complement(8513..8547)
                     /note="pHAGE 3' CDS"
     primer_bind     complement(8591..8614)
                     /note="pHAGE-A3550-3050"
     primer_bind     8744..8764
                     /note="pHAGE-S4001-4500"
     LTR             9081..9402
                     /note="3' LTR"
     misc_feature    9081..9182
                     /note="HIV nef"
     misc_feature    9131..9145
                     /note="HIV PPT"
                     /note="PolyPurine Tract"
     misc_feature    9148..9184
                     /note="dU3"
                     /note="1st Half of deleted U3"
     misc_feature    9204..9222
                     /note="dU3"
                     /note="2nd Half of deleted U3"
     misc_feature    9223..9319
                     /note="HIV R"
     misc_feature    9320..9402
                     /note="HIV U5"
     primer_bind     9434..9456
                     /note="pHAGE-S6001-6500"
     promoter        9605..9639
                     /note="P-Bla"
                     /note="Beta Lactamase Promoter"
     CDS             9658..10497
                     /gene="AMPr"
                     /product="beta-lactamase (mature form)"
                     /note="AMPr"
                     /note="Ampicillin Resistance Gene"
     primer_bind     complement(9707..9730)
                     /note="pHAGE-A6050-5550"
     primer_bind     9907..9931
                     /note="pHAGE-S6501-7000"
     primer_bind     complement(10176..10199)
                     /note="pHAGE-A6550-6050"
     primer_bind     10427..10451
                     /note="pHAGE-S7001-7500"
     primer_bind     complement(10687..10708)
                     /note="pHAGE-A7050-6550"
     rep_origin      10726..11259
                     /gene="ORI"
                     /note="ORI"
                     /note="Bacterial Origin of Replication"
     primer_bind     10931..10955
                     /note="pHAGE-S7501-8000"
     primer_bind     complement(11198..11219)
                     /note="pHAGE-A7550-7050"
     primer_bind     11434..11453
                     /note="pHAGE-S8001-8500"
     misc_feature    11496..11619
                     /gene="SV40 ori"
                     /note="SV40 ori"
     primer_bind     complement(11674..11700)
                     /note="pHAGE-A8050-7550"
     primer_bind     11939..11967
                     /note="pHAGE-S1-500"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtatg
     2221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgaag actgctgctc tctaccacgt
     2461 tagctcccct gtggcggagg atgactgtcc taagagctca taggcacggg gcaaggggag
     2521 cctggctgtc agctgcctgg gcttctagtc ttgtgctttt tgcacaccag tccagggaac
     2581 gaagaccact ggctttaaga tgcttcccca gctgtcccca gactctgcca gcaggggatt
     2641 ctctggtctg agcttaagtt gtgttctccc agccagggat gcccctgccc tttgatgtct
     2701 ccttgctgcc acacatttag ccgccctccc catgccagct tggggggagg gaagcagtgg
     2761 gggtagggag gtggctgggg cagctgggca actgtcccca cccgtccctg gcacggctct
     2821 gcccagtaca caaagagcaa agtgaatctt gtccccaccc ctgcagctga ggggctggag
     2881 gaggaaacgg ggaggcccac acagaagggg tggccaccgt ggggctgtcc atcactcagg
     2941 gctctcagag ggagtcaacc cagaaacaga caaagagggt gagtctgggc tgtgttctta
     3001 gctagtgaga ggtcccctag aggatgaagt agatgatgct aatgaggatg actggatgtc
     3061 acacccatga tgctattagg tcctcataat agcatagtga ggtggacagc tagtacctga
     3121 cccatctcac agatgaacat actaatgcct aacaaagcag aacgactcac gctgggtccc
     3181 agagctggcc agtggaagca ctgagacctc cacatactga aggcatggac tattgaccgc
     3241 tgttggtatt ggtctcatca ttgactatca ttaagtgttg gctgtttgca tgcttcctgc
     3301 ccagtggcag gttcaaagaa gcccgcagga agcgtgctcc tttctttccc agggcccgca
     3361 attgggctgg aagatagagc aacaaaaagc gcccatgtaa ctcatgggaa cattcatgtg
     3421 tgctgaatgg caggtgaagg tgccacagag aggctgagga tttcagaggg caccatgaac
     3481 tggagtgagg tcgcagagca ggtgccattg gctcttggcc tgtttgggtg ggtggcattc
     3541 agagaggtgg aaggtcagat gcactgttca cgcctgtaat ctcagcactt tggaaggcca
     3601 aggtaggagg atcacacgag gccaggagat caacgctgca gtgagctatg aagctgtgat
     3661 tgcaccactg cactgcagct tgggtaacag agtgagaccc tgtctctaaa taattaaata
     3721 aataaaataa aaataaaacc ggagaagtgg agagggattg gaggtgggct ttcacagagg
     3781 gagaaacggc ttaagtacag gccaaaaagt gagaaggctg cagacagggc tggtaggggg
     3841 agggggaaat ttggcacacc cagctaaagg tccttctgtg tctccttctc caaggaaccc
     3901 aagaccttca cttggttggt gtgagcactc caggaggcag gcaccctccc tcagccctca
     3961 agcaagcaaa aatgggttta aaaaaagaag gagaagaagc agcagcagca gccgccacag
     4021 agcttgtgac agctacagcc taagggcaac aggcagggga gaccaaggac cagaaagagc
     4081 agaggctttt tcaaagaaag agatccctct cccagcaccc agcgatggcg gcaaacccca
     4141 cccacagtgc ctgctaagaa caagtcccac gtgagaacaa catggccccc cgagatgccc
     4201 acagggaccc cgagatgcct gcagtgcttg gctctcccgc tggccagctg cccacctggc
     4261 tcaggcccag tactcgtgag tcagccgatc aatcccaatg ttgccaggat gatgggggcg
     4321 ggagtaaggg ccctggggga gggcaggggt gggcactgca ggcgagctgt ctcccacatc
     4381 tggcacctgc acacagctga ggccgagctg agaggatgct tctgtgggct ccccctcctc
     4441 ccggcacccc tcccctcctt tcactgtcct aggacactct ctggctgctg gagtcttagg
     4501 caaatattta aaggggcagc aagggggtga ggagggtggt gggagcaaac acttccctcc
     4561 ctttcttcct ccctgcgctt ctcaggggct ctcagttcag atgccatgct gttatgcaac
     4621 cttggggctg aaggccctcc agatggagag ggggacaggg gaacctgcca gctcatgacc
     4681 gaagggcagg gcccaggtgg gagggggctg gggcagggga caggaactgg ggtggcatgt
     4741 taaaggacag gaggctggtt gggcatggtg gctcacacct gtaatcctag cactttagga
     4801 ggccgagatc acttgagccc aggagttcaa gaccagcctg ggcaacatgg tgaaaactca
     4861 tctctataaa acaagcaaaa attagctggg cacaatggca tgcactggta gtcccagcta
     4921 cttgggatgc tgaggtgtga ggatcaccgg agtccaggag gtcaacgctg cagtgagcag
     4981 tgatctcgct actgcatgcc agcctgggtg ataaagtgag accctgtctc acaacaaaac
     5041 aaaacaaaac aaaacaaaag gataggaggt taagggagcg agcccaggcc tggactctgc
     5101 cacagtcacc tgagtttaga ggtgaaggga ctttagagac cacctggccc aaggagtgaa
     5161 agggaacctg agagaaaggg tgagccagcc caaagtcatt ggcagatttg acctcgtaaa
     5221 tacatagaga tggctttggg aaggcactag gaaagacaga gaaaagagaa ggagacagtc
     5281 ctcaaagctg atcgtatttg ggtgagtatt attctcaggg caaatttagg atcagaggat
     5341 gcagaaaggg gagtctagag gggtagagtg tagaccacag ggtgagtgag ctgattcgag
     5401 gatggggaga ctgggagccc accagtgacc agagccagcc ctgttcaggg ctgtccgggc
     5461 agaagaaagc agtgtcagac ctggaatctg ccatcagcac agcctgcaat tgacagacaa
     5521 gcccagagca aagaaggaag cactgcacat gagtaagagc ttgccaccag tggggacaga
     5581 gtttccagaa ttaggaaaat aatcactggg ggcaagtttg aggttggtac cagatatgtg
     5641 ggaggaggca aggtaaggga aagagtactt gaagttggaa ctggtccttg cagggaaatg
     5701 cacatttatg aaaccccgaa aactgatgtc aaagcacctc ctgccttggg cagagtcctc
     5761 tcagagtcta caggtgctgc ctccagaacc ctcttcctgg agcgcatccc tatgtatcta
     5821 gaaattctgc tgggaaatat gatggtcaga cccttggcca cctgaaaggt tcagggtggt
     5881 agaagaaaaa ggaaagccac agggcagcag gggcaggtgc cagcaaggaa ggcaggcacg
     5941 ccaggaagac acccatggtg agaagtgcag atggcccgag ggcaagtttg ctcaactcac
     6001 ccaggtttgc tcttgctggg gccaagagga ctcatgtgcc agggccaagg gcccttgggg
     6061 gctctcacag ggggcttatc tgggcttcgg ttctggaggg ccaggaacaa acaggcttca
     6121 aagccaaggg cttggctggc acacaggggg cttggtcctt cacctctgtc ccctctccct
     6181 acggacacat ataagaccct ggtcacacct gggagaggag gagaggagag catagcacct
     6241 gcaggtcgac tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc
     6301 ttgccaccat ggcgtcacgc atagggttgc gcatgcagct catgcgggag caggcgcagc
     6361 aggaggagca acgggagcgc atgcagcaac aggctgtcat gcattacatg cagcagcagc
     6421 agcagcagca acagcagcag ctcggagggc cgcccacccc ggccatcaat acccccgtcc
     6481 acttccagtc gccaccacct gtgcctgggg aggtgttgaa ggtgcagtcc tacctggaga
     6541 atcccacatc ctaccatctg cagcagtcgc agcatcagaa ggtgcgggag tacctgtccg
     6601 agacctatgg gaacaagttt gctgcccaca tcagcccagc ccagggctct ccgaaacccc
     6661 caccagccgc ctccccaggg gtgcgagctg gacacgtgct gtcctcctcc gctggcaaca
     6721 gtgctcccaa tagccccatg gccatgctgc acattggctc caaccctgag agggagttgg
     6781 atgatgtcat tgacaacatt atgcgtctgg acgatgtcct tggctacatc aatcctgaaa
     6841 tgcagatgcc caacacgcta cccctgtcca gcagccacct gaatgtgtac agcagcgacc
     6901 cccaggtcac agcctccctg gtgggcgtca ccagcagctc ctgccctgcg gacctgaccc
     6961 agaagcgaga gctcacagat gctgagagca gggccctggc caaggagcgg cagaagaaag
     7021 acaatcacaa cttaattgaa aggagacgaa ggttcaacat caatgaccgc atcaaggagt
     7081 tgggaatgct gatccccaag gccaatgacc tggacgtgcg ctggaacaag ggcaccatcc
     7141 tcaaggcctc tgtggattac atccggagga tgcagaagga cctgcaaaag tccagggagc
     7201 tggagaacca ctctcgccgc ctggagatga ccaacaagca gctctggctc cgtatccagg
     7261 agctggagat gcaggctcga gtgcacggcc tccctaccac ctccccgtcc ggcatgaaca
     7321 tggctgagct ggcccagcag gtggtgaagc aggagctgcc tagcgaagag ggcccagggg
     7381 aggccctgat gctgggggct gaggtccctg accctgagcc actgccagct ctgcccccgc
     7441 aagccccgct gcccctgccc acccagccac cgtccccatt ccatcacctg gacttcagcc
     7501 acagcctgag ctttgggggc agggaggacg agggtccccc gggctacccc gaacccctgg
     7561 cgccggggca tggctcccca ttccccagcc tgtccaagaa ggatctggac ctcatgctcc
     7621 tggacgactc actgctaccg ctggcctctg atccacttct gtccaccatg tcccccgagg
     7681 cctccaaggc cagcagccgc cggagcagct tcagcatgga ggagggcgat gtgctgaagg
     7741 taccgcgggc ccgggatcca ccggtcgcgg ccgccatgag cgagctgatt aaggagaaca
     7801 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     7861 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     7921 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     7981 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     8041 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     8101 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     8161 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     8221 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     8281 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     8341 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     8401 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     8461 ggcacaagct taattaaagg atcctaatca acctctggat tacaaaattt gtgaaagatt
     8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8881 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9061 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     9121 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9181 atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc tggttagacc
     9241 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9301 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9361 gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg attaaggaaa
     9421 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9481 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9841 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     9901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11281 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11341 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11401 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11461 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11521 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11581 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    11641 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    11701 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    11761 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    11821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11881 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11941 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12001 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
