LOCUS       pMV1_pEF1a-HGF          7062 bp ds-DNA     circular     07-JUL-2022
DEFINITION  .
ACCESSION   test                                                               
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     rep_origin      complement(1..589)
                     /label="ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             1335..2195
                     /label="AmpR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2208..2344
                     /label="rrnG terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2496..2567
                     /label="5' beta-globin insulator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      2572..2618
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    2636..2684
                     /label="synthetic polyadenylation signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2721..3899
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     intron          2952..3890
                     /label="EF-1-alpha intron A"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3970..3989
                     /label="20bp Gibson Overhang (for pJT312)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3990..3995
                     /label="Kozak"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3996..6182
                     /label="HGF (hepatocyte growth factor)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3996..6182
                     /label="Translation 3996-6182"
     CDS             5932..5943
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6183..6207
                     /label="25bp Gibson Overhang (for pJT312)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    6273..6394
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      6509..6555
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      6585..6721
                     /label="rrnG terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6766..6837
                     /label="3' beta-globin insulator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcattta aatttccgaa
      841 ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc ttgcaggcta
      901 cctctcgaac gaactatcgc aagtctcttg gccggccttg cgccttggct attgcttggc
      961 agcgcctatc gccaggtatt actccaatcc cgaatatccg agatcgggat cacccgagag
     1021 aagttcaacc tacatcctca atcccgatct atccgagatc cgaggaatat cgaaatcggg
     1081 gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc catatcgttg
     1141 cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc gtcagatatt
     1201 gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat tgatagtctg
     1261 atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag acaggatcag
     1321 caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1441 atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1501 agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1621 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
     1681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1741 ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1861 gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta actggcgaac
     1921 tacttactct agcttcccgg caacagttga tagactggat ggaggcggat aaagttgcag
     1981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2161 ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca ttggcgcaga
     2221 aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac
     2281 ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc
     2341 ttaagatccc gaatcgttta aactcgactc tggctctatc gaatctccgt cgtttcgagc
     2401 ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt
     2461 cagcttacct ttttggcagc gatcgcggct cccgacatct tggaccatta gctccacagg
     2521 tatcttcttc cctctagtgg tcataacagc agcttcagct acctctcaat tcaaaaaacc
     2581 cctcaagacc cgtttagagg ccccaagggg ttatgctatc aatcgttgcg ttacacacac
     2641 aaaaaaccaa cacacatcca tcttcgatgg atagcgattt tattatctaa ctgctgatcg
     2701 agtgtagcca gatctcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2761 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2821 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2881 gagaaccgta tataagtgca ctagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2941 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3001 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3061 ttcgggttgg aagtgggtgg gagagttcgt ggccttgcgc ttaaggagcc ccttcgcctc
     3121 gtgcttgagt tgtggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3181 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3241 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat cagcacactg
     3301 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3361 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3421 cccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3481 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3541 cagggagcac aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3601 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3661 cgccgtccag gcacctcgat tagttctcca gcttttggag tacgtcgtct ttaggttggg
     3721 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3781 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3841 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa
     3901 aactacccct aaaagccaaa agatctttgt cgatcctacc atccactcga cacacccgcc
     3961 agcggccgcG TAACCTCAAC TCCTGCGCCg ccaccatgtg ggtgaccaaa ctcctgccag
     4021 ccctgctgct gcagcatgtc ctcctgcatc tcctcctgct ccccatcgcc atcccctatg
     4081 cagagggaca aaggaaaaga agaaatacaa ttcatgaatt caaaaaatca gcaaagacta
     4141 ccctaatcaa aatagatcca gcactgaaga taaaaaccaa aaaagtgaat actgcagacc
     4201 aatgtgctaa tagatgtact aggaataaag gacttccatt cacttgcaag gcttttgttt
     4261 ttgataaagc aagaaaacaa tgcctctggt tccccttcaa tagcatgtca agtggagtga
     4321 aaaaagaatt tggccatgaa tttgacctct atgaaaacaa agactacatt agaaactgca
     4381 tcattggtaa aggacgcagc tacaagggaa cagtatctat cactaagagt ggcatcaaat
     4441 gtcagccctg gagttccatg ataccacacg aacacagctt tttgccttcg agctatcggg
     4501 gtaaagacct acaggaaaac tactgtcgaa atcctcgagg ggaagaaggg ggaccctggt
     4561 gtttcacaag caatccagag gtacgctacg aagtctgtga cattcctcag tgttcagaag
     4621 ttgaatgcat gacctgcaat ggggagagtt atcgaggtct catggatcat acagaatcag
     4681 gcaagatttg tcagcgctgg gatcatcaga caccacaccg gcacaaattc ttgcctgaaa
     4741 gatatcccga caagggcttt gatgataatt attgccgcaa tcccgatggc cagccgaggc
     4801 catggtgcta tactcttgac cctcacaccc gctgggagta ctgtgcaatt aaaacatgcg
     4861 ctgacaatac tatgaatgac actgatgttc ctttggaaac aactgaatgc atccaaggtc
     4921 aaggagaagg ctacaggggc actgtcaata ccatttggaa tggaattcca tgtcagcgtt
     4981 gggattctca gtatcctcac gagcatgaca tgactcctga aaatttcaag tgcaaggacc
     5041 tacgagaaaa ttactgccga aatccagatg ggtctgaatc accctggtgt tttaccactg
     5101 atccaaacat ccgagttggc tactgctccc aaattccaaa ctgtgatatg tcacatggac
     5161 aagattgtta tcgtgggaat ggcaaaaatt atatgggcaa cttatcccaa acaagatctg
     5221 gactaacatg ttcaatgtgg gacaagaaca tggaagactt acatcgtcat atcttctggg
     5281 aaccagatgc aagtaagctg aatgagaatt actgccgaaa tccagatgat gatgctcatg
     5341 gaccctggtg ctacacggga aatccactca ttccttggga ttattgccct atttctcgtt
     5401 gtgaaggtga taccacacct acaatagtca atttagacca tcccgtaata tcttgtgcca
     5461 aaacgaaaca attgcgagtt gtaaatggga ttccaacacg aacaaacata ggatggatgg
     5521 ttagtttgag atacagaaat aaacatatct gcggaggatc attgataaag gagagttggg
     5581 ttcttactgc acgacagtgt ttcccttctc gagacttgaa agattatgaa gcttggcttg
     5641 gaattcatga tgtccacgga agaggagatg agaaatgcaa acaggttctc aatgtttccc
     5701 agctggtata tggccctgaa ggatcagatc tggttttaat gaagcttgcc aggcctgctg
     5761 tcctggatga ttttgttagt acgattgatt tacctaatta tggatgcaca attcctgaaa
     5821 agaccagttg cagtgtttat ggctggggct acactggatt gatcaactat gatggcctat
     5881 tacgagtggc acatctctat ataatgggaa atgagaaatg cagccagcat catcgaggga
     5941 aggtgactct gaatgagtct gaaatatgtg ctggggctga aaagattgga tcaggaccat
     6001 gtgaggggga ttatggtggc ccacttgttt gtgagcaaca taaaatgaga atggttcttg
     6061 gtgtcattgt tcctggtcgt ggatgtgcca ttccaaatcg tcctggtatt tttgtccgag
     6121 tagcatatta tgcaaaatgg atacacaaaa ttattttaac atataaggta ccacagtcat
     6181 agGCCTAATA AAGAGCTCAG ATGCATGgga tccgcaggcc tctgctagct tgactgactg
     6241 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac
     6301 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     6421 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     6481 tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc
     6541 ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa
     6601 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     6661 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     6721 aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt
     6781 cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc
     6841 tcattggtta ccttgggcta tcgaaactta attaaccagt caagtcagct acttggcgag
     6901 atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc
     6961 ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca
     7021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tt
//