LOCUS       pMV2_AAV-RapaInducible- 7131 bp ds-DNA     circular     01-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(161..180)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      974..1562
                     /label="ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1463..1482
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     repeat_region   1624..1764
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_region   1635..1764
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    1779..1790
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    1779..1808
                     /label="extra sites for pos feedback"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    1791..1796
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1797..1808
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    1815..1894
                     /label="pSCP1 I think"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     source          1815..1900
                     /label="source"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     regulatory      1904..1909
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1913..1939
                     /label="c-myc NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1940..2203
                     /label="FRB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2207..2224
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2660..2668
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2669..2722
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2723..2749
                     /label="c-myc NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             2756..3118
                     /label="ZFHD1 (DNA binding domain)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             3125..3445
                     /label="FKBP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     source          3455..3471
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3455..3471
                     /label="1x sNRP-1 term"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    3478..3489
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3490..3495
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3496..3507
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3508..3513
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3514..3525
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3526..3531
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3532..3543
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3544..3549
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3550..3561
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3562..3567
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3568..3579
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3580..3585
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3586..3597
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3598..3603
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3604..3615
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3616..3621
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    3622..3633
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        3650..3763
                     /label="minimal IL-2 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3767..3772
                     /label="Kozak"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3773..5959
                     /label="HGF (hepatocyte growth factor)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3773..5959
                     /label="Translation 3773-5959"
     misc_feature    5252..5257
                     /label="cleavage site (alpha-beta)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             5709..5720
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5960..6012
                     /label="min rabbit B globin UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     repeat_region   6029..6158
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     repeat_region   6029..6169
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      6244..6699
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(6331..6350)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     6541..6562
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(6716..6735)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     6829..6851
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(6883..6901)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        6969..7073
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             join(7074..7131,1..803)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
       61 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      121 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      181 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      241 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      301 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      361 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      421 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      481 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      541 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      601 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      661 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      721 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      781 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      841 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1081 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1621 tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
     1681 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
     1741 ccaactccat cactaggggt tcctgcggcc gcacgcgtTA ATGATGGGCG CtacAGTAAT
     1801 GATGGGCGGA ATTCctcgag gtacttatat aagggggtgg gggcgcgttc gtcctcagtc
     1861 gcgatcgaac actcgagccg agcagacgtg cctaaccggt gccgccacca tgcctgctgc
     1921 caagagggtc aagttggact ggcatgaagg cctggaagag gcatctcgtt tgtactttgg
     1981 ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta tgatggaacg
     2041 gggcccccag actctgaagg aaacatcTtt taatcaggcc tatggtcgTg atttaatgga
     2101 ggcccaagag tggtgcagga agtacatgaa atcagggaat gtcaaggacc tcacccaagc
     2161 ctgggacctc tattatcatg tgttccgacg aatctcaaag cagtctggAT CCggaTCAgg
     2221 ctccGAGGGC CAGAGCGACG AGAGAGCCCT GCTGGACCAG CTGCACACCC TGCTGAGCAA
     2281 CACCGACGCC ACCGGCCTGG AGGAGATCGA CAGAGCCCTG GGCATCCCCG AGCTGGTGAA
     2341 CCAGGGCCAG GCCCTGGAGC CCAAGCAGGG AGGAGGAGGT AGCggaCACG AGAAGTTCCC
     2401 CAGCGACCTG GACCTGGACA TGTTCAACGG CAGCCTGGAG TGCGACATGG AGAGCATCAT
     2461 CAGAAGCGAG CTGATGGACG CCGACGGCCT GGACTTCAAC TTCGACAGCG GAGGAGGAGG
     2521 TAGCggaTTC GTGACCCTGA AGGACGTGGG CATGGACTTC ACCCTGGGCG ACTGGGAGCA
     2581 GCTGGGCCTG GAGCAGGGCG ACACCTTCTG GGACACCGCC CTGGACAACT GCCAGGACCT
     2641 GTTCCTGCTG GACCCCCCCg gAtctgGAga gggAaggggA agcctcctaa cttgcggaga
     2701 Tgtcgaggag aatcctggcc cacctgcCgc TaagCGTgtc aagttggact ctagagaacg
     2761 cccGtatgct tgccctgtcg agtcctgcga tcgccgcttt tctcgctcgg atgagcttac
     2821 ccgccatatc cgcatccaca caggccagaa gcccttccag tgtcgaatct gcatgcgtaa
     2881 cttcagtcgt agtgaccacc ttaccaccca catccgcacc cacacaggcg gcggccgcag
     2941 gaggaagaaa cgcaccagca tagagacTaa catccgtgtg gccttagaga agagtttctt
     3001 ggagaatcaa aagcctacct cggaagagat cactatgatt gctgatcagc tcaatatgga
     3061 aaaagaggtg attcgtgttt ggttctgtaa ccgccgccag aaagaaaaaa gaatcaacaa
     3121 gggcggcgtg caggtggaga ctatctcccc aggcgacggg cgcaccttcc ccaagcgcgg
     3181 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc
     3241 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
     3301 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
     3361 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
     3421 Tgatgtggag cttctaaaac tggaaTAAca tatgaaataa aatacgaaat gCGTACGTAA
     3481 TGATGGGCGC TaaatTAATG ATGGGCGgag ttGTAATGAT GGGCGCTCGt tTAATGATGG
     3541 GCGTCTAGCT AATGATGGGC GggCGAtTAA TGATGGGCGa agGACTAATG ATGGGCGCag
     3601 cgGTAATGAT GGGCGatcAG cTAATGATGG GCGACGCGAA TTAATTCAAC ATTTTGACAC
     3661 CCCCATAATA TTTTTCCAGA ATTAACAGTA TAAATTGCAT CTCTTGTTCA AGAGTTCCCT
     3721 ATCACTCTCT TTAATCACTA CTCACAGTAA CCTCAACTCC TGCgccgcca ccatgtgggt
     3781 gaccaaactc ctgccagccc tgctgctgca gcatgtcctc ctgcatctcc tcctgctccc
     3841 catcgccatc ccctatgcag agggacaaag gaaaagaaga aatacaattc atgaattcaa
     3901 aaaatcagca aagactaccc taatcaaaat agatccagca ctgaagataa aaaccaaaaa
     3961 agtgaatact gcagaccaat gtgctaatag atgtactagg aataaaggac ttccattcac
     4021 ttgcaaggct tttgtttttg ataaagcaag aaaacaatgc ctctggttcc ccttcaatag
     4081 catgtcaagt ggagtgaaaa aagaatttgg ccatgaattt gacctctatg aaaacaaaga
     4141 ctacattaga aactgcatca ttggtaaagg acgcagctac aagggaacag tatctatcac
     4201 taagagtggc atcaaatgtc agccctggag ttccatgata ccacacgaac acagcttttt
     4261 gccttcgagc tatcggggta aagacctaca ggaaaactac tgtcgaaatc ctcgagggga
     4321 agaaggggga ccctggtgtt tcacaagcaa tccagaggta cgctacgaag tctgtgacat
     4381 tcctcagtgt tcagaagttg aatgcatgac ctgcaatggg gagagttatc gaggtctcat
     4441 ggatcataca gaatcaggca agatttgtca gcgctgggat catcagacac cacaccggca
     4501 caaattcttg cctgaaagat atcccgacaa gggctttgat gataattatt gccgcaatcc
     4561 cgatggccag ccgaggccat ggtgctatac tcttgaccct cacacccgct gggagtactg
     4621 tgcaattaaa acatgcgctg acaatactat gaatgacact gatgttcctt tggaaacaac
     4681 tgaatgcatc caaggtcaag gagaaggcta caggggcact gtcaatacca tttggaatgg
     4741 aattccatgt cagcgttggg attctcagta tcctcacgag catgacatga ctcctgaaaa
     4801 tttcaagtgc aaggacctac gagaaaatta ctgccgaaat ccagatgggt ctgaatcacc
     4861 ctggtgtttt accactgatc caaacatccg agttggctac tgctcccaaa ttccaaactg
     4921 tgatatgtca catggacaag attgttatcg tgggaatggc aaaaattata tgggcaactt
     4981 atcccaaaca agatctggac taacatgttc aatgtgggac aagaacatgg aagacttaca
     5041 tcgtcatatc ttctgggaac cagatgcaag taagctgaat gagaattact gccgaaatcc
     5101 agatgatgat gctcatggac cctggtgcta cacgggaaat ccactcattc cttgggatta
     5161 ttgccctatt tctcgttgtg aaggtgatac cacacctaca atagtcaatt tagaccatcc
     5221 cgtaatatct tgtgccaaaa cgaaacaatt gcgagttgta aatgggattc caacacgaac
     5281 aaacatagga tggatggtta gtttgagata cagaaataaa catatctgcg gaggatcatt
     5341 gataaaggag agttgggttc ttactgcacg acagtgtttc ccttctcgag acttgaaaga
     5401 ttatgaagct tggcttggaa ttcatgatgt ccacggaaga ggagatgaga aatgcaaaca
     5461 ggttctcaat gtttcccagc tggtatatgg ccctgaagga tcagatctgg ttttaatgaa
     5521 gcttgccagg cctgctgtcc tggatgattt tgttagtacg attgatttac ctaattatgg
     5581 atgcacaatt cctgaaaaga ccagttgcag tgtttatggc tggggctaca ctggattgat
     5641 caactatgat ggcctattac gagtggcaca tctctatata atgggaaatg agaaatgcag
     5701 ccagcatcat cgagggaagg tgactctgaa tgagtctgaa atatgtgctg gggctgaaaa
     5761 gattggatca ggaccatgtg agggggatta tggtggccca cttgtttgtg agcaacataa
     5821 aatgagaatg gttcttggtg tcattgttcc tggtcgtgga tgtgccattc caaatcgtcc
     5881 tggtattttt gtccgagtag catattatgc aaaatggata cacaaaatta ttttaacata
     5941 taaggtacca cagtcatagG CCTAATAAAG AGCTCAGATG CATGCATCAG AGTGTGTTGG
     6001 TTTTTTGTGT GTcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     6061 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     6121 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     6181 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     6241 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6421 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     6481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6541 gtggactctt gttccaaact ggaacaacac tcaacTctat ctcgggctat tcttttgatt
     6601 tataagggat tttgccgatt tcggTctatt ggttaaaaaa tgagctgatt taacaaaaat
     6661 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     6721 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6781 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gggagctgca
     6841 tgtgtcagag gttttcaccg tcatcaccga aacgcgcaaa gggcctcgtg atacgcctat
     6901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt c
//