LOCUS       pJT151:_lenti_pEF-rTetR 9193 bp ds-DNA     circular     07-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(196..205)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    274..454
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1031..1037)
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1125..1358
                     /label="RRE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1889..1903
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2055..2085
                     /label="MCS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2113..3074
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3076..3285
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3295..3297
                     /label="Translation 3295-3297"
     misc_feature    3298..3915
                     /label="rTetR(SE-G72P)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3298..3915
                     /label="Translation 3298-3915"
     primer          3782..3803
                     /label="JT836"
                     /note="sequence: ccgattctatgcccccacttct"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3894..3912)
                     /label="JT827"
                     /note="sequence: gccgctttcgcactttagc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3916..3981
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3916..4002
                     /label="Translation 3916-4002"
     primer          3984..4002
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3985..4002
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4003..4215
                     /label="krabDMS089"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4216..4221
                     /label="Translation 4216-4221"
     misc_feature    4216..4224
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(4216..4235)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4222..4224
                     /label="Translation 4222-4224"
     misc_feature    4225..4278
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4225..4278
                     /label="Translation 4225-4278"
     primer          complement(4260..4278)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             4279..4986
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4279..4986
                     /label="Translation 4279-4986"
     misc_feature    4987..5004
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4987..5004
                     /label="Translation 4987-5004"
     CDS             5005..5403
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             5005..5403
                     /label="Translation 5005-5403"
     misc_recomb     5423..5456
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     misc_feature    5502..6102
                     /label="wPRE"
                     /ApEinfo_revcolor=#0060ff
                     /ApEinfo_fwdcolor=#0060ff
     misc_feature    6292..6306
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6629..6809
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(6853..7535)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     misc_feature    complement(6856..7475)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(6880..6889)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(7633..8292)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(8532..8560)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    8886..256
                     /label="pU6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1801 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     1861 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1981 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2041 agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt
     2101 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2161 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2221 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2281 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2341 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     2401 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     2461 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     2521 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     2581 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     2641 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     2701 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     2761 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     2821 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     2881 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     2941 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3001 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3061 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3121 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3181 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3241 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAgc caccATGtct
     3301 agactggaca agagcaaaAT Aataaacgga gctctggaat tactcaatgg tgtcggtatc
     3361 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     3421 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     3481 catcataccc acTCCtgccc cctggaaCCC gagtcatggc aagactttct gcggaacaac
     3541 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     3601 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     3661 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     3721 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     3781 accgattcta tgcccccact tctgAAAcaa gcaattgagc tgttcgaccg gcagggagcc
     3841 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     3901 tgcgaaagcg gcggtgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     3961 tacaaggatg acgatgacaa gGGTggATCC ggaTCAggct ccGATGCTAA GTCTCTGACT
     4021 GCCTGGTCCA GGACCCTTGT GACCTTCAAG GACGTCTTCG TTGATTTTAC GCGGGAAGAA
     4081 TGGAAACTAC TAGACACAGC ACAACAAATA GTATACAGAA ACGTAATGCT AGAAAACTAC
     4141 AAAAACCTAG TAAGCCTAGG ATACCAACTA ACAAAACCAG ACGTAATACT AAGACTAGAA
     4201 AAAGGAGAAG AACCAggAtc tgGAgagggA aggggAagcc tcctaacttg cggagaTgtc
     4261 gaggagaatc ctggcccaat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     4321 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4381 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4441 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     4501 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     4561 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4621 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     4681 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     4741 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     4801 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     4861 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     4921 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     4981 tacaagggta gcggcagcgg tagcatggcc aagcctttgt ctcaagaaga atccaccctc
     5041 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     5101 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     5161 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     5221 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     5281 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     5341 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     5401 taagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
     5461 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     5521 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5581 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5641 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5701 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5761 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5821 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5881 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5941 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6001 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6061 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6121 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6181 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6241 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6301 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6361 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     6421 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     6481 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6541 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6601 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6781 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     6841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6901 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6961 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7021 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7081 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7141 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7201 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7261 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7321 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7381 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7441 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7501 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7561 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7621 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7681 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7741 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7801 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7861 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7921 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7981 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8041 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8101 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8161 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8221 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8281 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8341 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8401 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8461 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8521 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8581 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcttaag cggtcgacgg
     8641 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     8701 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     8761 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8821 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     8881 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8941 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9001 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9061 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9121 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9181 cagtacatga cct
//