LOCUS       pJT218_lenti_pEF-rTetR( 9130 bp ds-DNA     circular     09-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(496..505)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    574..754
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1331..1337)
                     /label="TATA box"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1425..1658
                     /label="RRE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2189..2203
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2355..2385
                     /label="MCS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2413..3374
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3376..3585
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3595..3597
                     /label="Translation 3595-3597"
     CDS             3595..5640
                     /label="Translation 3595-5640"
     misc_feature    3598..4215
                     /label="rTetR(SE-G72P)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             3598..4215
                     /label="Translation 3598-4215"
     misc_feature    4216..4281
                     /label="3XFLAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4216..4298
                     /label="Translation 4216-4298"
     misc_feature    4285..4298
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4303..4452
                     /label="VP64"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4453..4458
                     /label="Translation 4453-4458"
     misc_feature    4453..4461
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4459..4461
                     /label="Translation 4459-4461"
     misc_feature    4462..4515
                     /label="T2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4462..4515
                     /label="Translation 4462-4515"
     CDS             4516..5223
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             4516..5223
                     /label="Translation 4516-5223"
     misc_feature    5224..5241
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5224..5241
                     /label="Translation 5224-5241"
     CDS             5242..5640
                     /label="BSD"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             5242..5640
                     /label="Translation 5242-5640"
     misc_recomb     5660..5693
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    5739..6339
                     /label="wPRE"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_fwdcolor="#0060ff"
     misc_feature    6529..6543
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6866..7046
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(7090..7772)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
     misc_feature    complement(7093..7712)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(7117..7126)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7870..8529)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(8769..8797)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    9123..556
                     /label="pU6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
       61 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      361 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      421 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      481 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      541 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      721 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      781 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      841 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      901 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      961 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1021 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1081 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1141 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1201 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1261 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1321 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1381 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1441 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1501 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1561 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1621 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1681 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1741 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1801 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1861 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1921 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1981 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2041 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2101 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     2161 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2221 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2281 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2341 agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt
     2401 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2461 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2521 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2581 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2641 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     2701 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     2761 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     2821 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     2881 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     2941 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     3001 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     3061 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     3121 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     3181 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     3241 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3301 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3361 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3421 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3481 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3541 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAgc caccATGtct
     3601 agactggaca agagcaaaAT Aataaacgga gctctggaat tactcaatgg tgtcggtatc
     3661 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     3721 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     3781 catcataccc acTCCtgccc cctggaaCCC gagtcatggc aagactttct gcggaacaac
     3841 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     3901 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     3961 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     4021 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     4081 accgattcta tgcccccact tctgAAAcaa gcaattgagc tgttcgaccg gcagggagcc
     4141 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     4201 tgcgaaagcg gcggtgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4261 tacaaggatg acgatgacaa gGGTggATCC ggaTCAggCT CCgacgcatt ggacgatttt
     4321 gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg
     4381 gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac
     4441 ctggacatgc tgggAtctgG AgagggAagg ggAagcctcc taacttgcgg agaTgtcgag
     4501 gagaatcctg gcccaatggt gagcaagggc gaggaggata acatggccat catcaaggag
     4561 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4621 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4681 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     4741 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     4801 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4861 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     4921 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     4981 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     5041 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5101 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     5161 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     5221 aagggtagcg gcagcggtag catggccaag cctttgtctc aagaagaatc caccctcatt
     5281 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     5341 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     5401 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     5461 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     5521 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     5581 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     5641 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
     5701 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
     5761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6121 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6181 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6301 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     6361 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     6421 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     6481 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6541 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6601 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6661 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6721 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6781 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6841 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6901 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6961 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7021 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     7081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7741 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7801 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7861 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7921 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7981 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8041 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8101 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8161 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8221 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8281 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8341 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8401 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8461 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8521 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8581 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8641 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8701 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8761 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8821 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcttaagcgg tcgacggatc
     8881 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8941 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9001 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9061 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     9121 actagttatt
//