LOCUS       pJT230:_lenti_pEF-rTetR 9220 bp ds-DNA     circular     07-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(196..205)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    274..454
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1031..1037)
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1125..1358
                     /label="RRE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1889..1903
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2055..2085
                     /label="MCS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2113..3074
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3076..3285
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3295..3297
                     /label="Translation 3295-3297"
     misc_feature    3298..3915
                     /label="rTetR(SE-G72P)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3298..3915
                     /label="Translation 3298-3915"
     primer          3782..3803
                     /label="JT836"
                     /note="sequence: ccgattctatgcccccacttct"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3894..3912)
                     /label="JT827"
                     /note="sequence: gccgctttcgcactttagc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3916..3981
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3916..4002
                     /label="Translation 3916-4002"
     primer          3984..4002
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3985..4002
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4003..4242
                     /label="NCOA3"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4243..4248
                     /label="Translation 4243-4248"
     misc_feature    4243..4251
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(4243..4262)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4249..4251
                     /label="Translation 4249-4251"
     misc_feature    4252..4305
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4252..4305
                     /label="Translation 4252-4305"
     primer          complement(4287..4305)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             4306..5013
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4306..5013
                     /label="Translation 4306-5013"
     misc_feature    5014..5031
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             5014..5031
                     /label="Translation 5014-5031"
     CDS             5032..5430
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             5032..5430
                     /label="Translation 5032-5430"
     misc_recomb     5450..5483
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     misc_feature    5529..6129
                     /label="wPRE"
                     /ApEinfo_revcolor=#0060ff
                     /ApEinfo_fwdcolor=#0060ff
     misc_feature    6319..6333
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6656..6836
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(6880..7562)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     misc_feature    complement(6883..7502)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(6907..6916)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(7660..8319)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(8559..8587)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    8913..256
                     /label="pU6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1801 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     1861 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1981 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2041 agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt
     2101 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2161 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2221 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2281 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2341 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     2401 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     2461 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     2521 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     2581 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     2641 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     2701 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     2761 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     2821 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     2881 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     2941 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3001 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3061 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3121 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3181 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3241 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAgc caccATGtct
     3301 agactggaca agagcaaaAT Aataaacgga gctctggaat tactcaatgg tgtcggtatc
     3361 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     3421 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     3481 catcataccc acTCCtgccc cctggaaCCC gagtcatggc aagactttct gcggaacaac
     3541 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     3601 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     3661 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     3721 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     3781 accgattcta tgcccccact tctgAAAcaa gcaattgagc tgttcgaccg gcagggagcc
     3841 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     3901 tgcgaaagcg gcggtgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     3961 tacaaggatg acgatgacaa gGGTggATCC ggaTCAggct ccCTTAGAAA TAGTTTGGAT
     4021 GATTTGGTCG GGCCACCTAG TAATTTGGAA GGGCAATCTG ATGAACGGGC ACTCCTTGAT
     4081 CAATTGCATA CGTTGCTCTC AAATACAGAT GCTACAGGGT TGGAAGAAAT TGATAGGGCT
     4141 CTCGGGATAC CAGAACTTGT TAATCAAGGG CAAGCACTCG AACCAAAACA AGATGCTTTT
     4201 CAAGGACAAG AAGCCGCGGT TATGATGGAT CAAAAGGCTG GTggAtctgG AgagggAagg
     4261 ggAagcctcc taacttgcgg agaTgtcgag gagaatcctg gcccaatggt gagcaagggc
     4321 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     4381 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     4441 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     4501 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     4561 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     4621 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     4681 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     4741 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     4801 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     4861 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     4921 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     4981 cactccaccg gcggcatgga cgagctgtac aagggtagcg gcagcggtag catggccaag
     5041 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     5101 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     5161 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     5221 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     5281 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     5341 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     5401 ctgccctctg gttatgtgtg ggagggctaa gaattcgtcg agggacctaa taacttcgta
     5461 tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc
     5521 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5581 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5641 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5701 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5761 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5821 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5881 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5941 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6001 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6061 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6121 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     6181 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6241 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6301 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6361 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6421 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6481 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6541 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6601 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8641 gccacctgac gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact
     8701 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     8761 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     8821 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     8881 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     8941 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9061 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9121 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9181 ggtaaatggc ccgcctggca ttatgcccag tacatgacct
//