LOCUS       Exported               15716 bp ds-DNA     circular SYN 08-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 8, 2022 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(24..41)
                     /label=SP6
     CDS             934..1794
                     /label=bla (AmpR)
     CDS             1132..1791
                     /label=AmpR
     promoter        2932..3128
                     /label=SV40 early promoter
     polyA_signal    complement(4841..5032)
                     /label=SV40 late polyA
     repeat_region   7227..7406
                     /label=cPPT
     misc_feature    7430..7670
                     /label=truncated UCOE
     misc_feature    complement(7444..7452)
                     /label=CTCF binding site
     misc_feature    7619..7632
                     /label=CTCF binding site
     misc_feature    7671..8109
                     /label=truncated UCOE
     promoter        8111..9288
                     /label=EF-1-alpha promoter
     intron          8342..9280
                     /label=EF-1-alpha intron A
     protein_bind    9343..9344
                     /label=attB1
     misc_feature    9344..9643
                     /label=ZIM3 (1-100)
     protein_bind    complement(9645..9669)
                     /label=attB2
     CDS             9689..9709
                     /label=SV40 NLS
     misc_feature    9758..9767
                     /label=vertebrate consensus sequence for strong initia
     CDS             9764..13867
                     /label=dCas9
     CDS             13871..13897
                     /label=HA
     CDS             13916..13936
                     /label=SV40 NLS
     CDS             13943..13963
                     /label=SV40 NLS
     misc_feature    13973..14029
                     /label=P2A
     misc_feature    14030..14737
                     /label=mCherry
     misc_feature    14738..14745
                     /label=AsiSI
     misc_feature    14746..14753
                     /label=SbfI
     enhancer        14782..15373
                     /label=WPRE
ORIGIN
        1 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
       61 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
      121 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
      181 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
      241 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
      301 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      361 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      421 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      481 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      541 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      781 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1141 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1441 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1801 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2101 ccaaatactg tctttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2701 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2761 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     2821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2881 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2941 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3001 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3061 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3121 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     3181 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     3241 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     3301 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     3361 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     3421 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     3481 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     3541 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     3601 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     3661 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     3721 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     3781 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     3841 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     3901 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     3961 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     4021 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     4081 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     4141 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     4201 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     4261 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     4321 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     4381 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     4441 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     4501 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     4561 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     4621 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     4681 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     4741 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     4801 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4861 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     5041 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     5101 ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     5161 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     5221 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5281 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     5341 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     5401 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     5461 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     5521 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     5581 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     5641 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5821 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     5881 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     5941 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6001 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6061 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     6121 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     6181 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     6241 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     6301 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     6361 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6421 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6481 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6541 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6601 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6661 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt gaaagatacc
     6721 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     6781 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     6841 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     6901 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     6961 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7021 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7081 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7141 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     7201 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
     7261 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     7321 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     7381 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc gggaggtggt
     7441 ccctgcagtt acgccaatga taacccccgc cagaaaaatc ttagtagcct tccctttttg
     7501 ttttccgtgc cccaactcgg cggattgact cggccccttc cggaaacacc cgaatcaact
     7561 tctagtcaaa ttattgttca cgccgcaatg acccacccct ggcccgcgtc tgtggaactg
     7621 acccctggtg tacaggagag ttcgctgctg aaagtggtcc caaaggggta ctagttttta
     7681 agctcccaac tccccctccc ccagcgtctg gaggattcca caccctcgca ccgcaggggc
     7741 gaggaagtgg gcggagtccg gttttggcgc cagccgctga ggctgccaag cagaaaagcc
     7801 accgctgagg agactccggt cactgtcctc gccccgcctc ccccttccct ccccttgggg
     7861 accaccgggc gccacgccgc gaacggtaag tgccgcggtc gtcggcgcct ccgccctccc
     7921 cctagggccc caattcccag cgggcgcggc gcgcggcccc tccccccgcc gggcgcgcgc
     7981 ccgctgcccc gcccttcgtg gccgcccggc gtgggcggtg ccacccctcc ccccggcggc
     8041 ccccctgcag ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta
     8101 ggcgcggagg ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     8161 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     8221 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     8281 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     8341 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     8401 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     8461 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     8521 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     8581 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     8641 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     8701 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     8761 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     8821 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     8881 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     8941 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     9001 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     9061 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     9121 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     9181 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     9241 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgga attctcacgc
     9301 gtctgcagga tatcaagctt gcggtaccgc gggcccggcc accatgaaca attcccaggg
     9361 aagagtgacc ttcgaggatg tcactgtgaa cttcacccag ggggagtggc agcggctgaa
     9421 tcccgaacag agaaacttgt acagggatgt gatgctggag aattacagca accttgtctc
     9481 tgtgggacaa ggggaaacca ccaaacccga tgtgatcttg aggttggaac aaggaaagga
     9541 gccatggttg gaggaagagg aagtgctggg aagtggccgt gcagaaaaaa atggggacat
     9601 tggagggcag atttggaagc caaaggatgt gaaagagagt ctctacccaa ctttcttgta
     9661 caaagtggtt ggtggatctg gaggttctcc caagaagaag cgcaaggtgg gacgcgtctg
     9721 caggatatca agcttgcggt accgcgggcc cgggatcgcc accatggaca agaagtacag
     9781 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     9841 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     9901 cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc ggctgaagag
     9961 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
    10021 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
    10081 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
    10141 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
    10201 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
    10261 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
    10321 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
    10381 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
    10441 cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct
    10501 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
    10561 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
    10621 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
    10681 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
    10741 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
    10801 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca tcgatggcgg
    10861 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
    10921 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
    10981 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
    11041 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
    11101 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
    11161 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
    11221 cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
    11281 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg agctgaccaa
    11341 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
    11401 agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
    11461 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
    11521 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
    11581 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
    11641 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
    11701 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
    11761 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
    11821 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
    11881 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
    11941 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
    12001 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
    12061 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
    12121 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
    12181 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
    12241 ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt
    12301 tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga accggggcaa
    12361 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcgccagct
    12421 gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg ccgagagagg
    12481 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
    12541 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
    12601 cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
    12661 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
    12721 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
    12781 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
    12841 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
    12901 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
    12961 cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg tgcggaaagt
    13021 gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
    13081 caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa agaaggactg
    13141 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
    13201 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
    13261 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
    13321 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
    13381 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
    13441 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
    13501 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca aacactacct
    13561 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
    13621 tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca gagagcaggc
    13681 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
    13741 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
    13801 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
    13861 aggcgacgcc tatccctatg acgtgcccga ttatgccagc ctgggcagcg gctcccccaa
    13921 gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtgagcggcc gcgctactaa
    13981 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggacctg tgagcaaggg
    14041 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
    14101 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
    14161 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
    14221 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
    14281 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
    14341 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
    14401 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
    14461 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
    14521 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
    14581 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
    14641 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
    14701 ccactccacc ggcggcatgg acgagctgta caagtaagcg atcgccctgc aggcatgcaa
    14761 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    14821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    14881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    14941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    15001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    15061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    15121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    15181 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    15241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    15301 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    15361 cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa
    15421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    15481 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    15541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    15601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    15661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagc
//
