LOCUS       pNM1129_-_pSFFV::Zim3- 15055 bp ds-DNA     circular     12-MAY-2022
DEFINITION  .
KEYWORDS    "creator:Jessica Bonnar" "marker:AmpR"                             
FEATURES             Location/Qualifiers
     promoter        29..345
                     /label="SV40 promoter"
     promoter        149..345
                     /label="SV40 early promoter"
     promoter        149..345
                     /label="SV40 early promoter"
     primer          complement(2019..2038)
                     /label="pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
     polyA_signal    complement(2058..2249)
                     /label="SV40 late polyA"
     primer          complement(2069..2085)
                     /label="EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
     primer          complement(2156..2175)
                     /label="SV40pA-R"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     repeat_region   4444..4623
                     /label="cPPT"
     misc_feature    4647..5234
                     /label="truncated UCOE"
     misc_feature    complement(4661..4669)
                     /label="CTCF binding site"
     misc_feature    4836..4849
                     /label="CTCF binding site"
     misc_feature    4845..4865
                     /label="CL77"
     misc_feature    complement(5130..5149)
                     /label="CTCF binding site"
     misc_feature    5169..5170
                     /label="cloning scar: cccctccccccgccgggcgcgcgcccgctgccc"
     promoter        5245..5736
                     /label="SFFV"
     misc_feature    5740..5764
                     /label="Overlap"
     protein_bind    5810..5811
                     /label="attB1"
     misc_feature    5810..5836
                     /label="Tm 71.1˚C"
     misc_feature    5811..6110
                     /label="ZIM3 (1-100)"
     CDS             5811..11248
                     /label="Translation 5811-11248"
     protein_bind    complement(6112..6136)
                     /label="attB2"
     misc_recomb     complement(6115..6135)
                     /label="attB2"
     misc_feature    complement(6115..6135)
                     /label="Gateway Recomb2"
     CDS             6156..6176
                     /label="SV40 NLS"
     misc_feature    6225..6234
                     /label="vertebrate consensus sequence for strong initia"
     CDS             6231..10334
                     /label="dCas9"
     CDS             10338..10364
                     /label="HA"
     CDS             10383..10403
                     /label="SV40 NLS"
     misc_feature    complement(10401..10430)
                     /label="Tm 71.1˚C"
     CDS             10410..10430
                     /label="SV40 NLS"
     misc_feature    10438..10439
                     /label="to keep 2?"
     misc_feature    10438..10495
                     /label="Overlap"
     misc_feature    10440..10473
                     /label="loxp2272"
     misc_feature    10476..10532
                     /label="P2A"
     misc_feature    10533..11248
                     /label="GFP"
     primer          10545..10562
                     /label="oMJ0174_seq_GFP_fw_12"
                     /note="sequence: gaggagctgttcaccggg"
     primer          complement(10576..10596)
                     /label="EGFP-N"
                     /note="sequence: CGTCGCCGTCCAGCTCGACCA"
     primer          11183..11204
                     /label="EGFP-C-For"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
     misc_feature    11249..11282
                     /label="loxp2272"
     misc_feature    11288..11293
                     /label="double stop after Cre recombination"
     misc_feature    11294..11301
                     /label="AsiSI"
     misc_feature    11302..11309
                     /label="SbfI"
     enhancer        11338..11929
                     /label="WPRE"
     promoter        complement(12295..12313)
                     /label="SP6 promoter"
     primer_bind     complement(12296..12313)
                     /label="SP6"
     primer          12955..12977
                     /label="3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
     CDS             13206..14066
                     /label="bla (AmpR)"
     CDS             13404..14063
                     /label="AmpR"
ORIGIN
        1 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
       61 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      301 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
      361 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
      421 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
      481 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
      541 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
      601 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
      661 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
      721 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
      781 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
      841 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
      901 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
      961 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     1021 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     1081 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     1141 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     1201 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     1261 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     1321 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     1381 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     1441 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     1501 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     1561 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     1621 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     1681 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     1741 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     1801 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     1861 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     1921 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     1981 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     2041 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2101 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2221 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     2281 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     2341 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     2401 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     2461 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     2521 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     2581 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     2641 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     2701 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     2761 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     2821 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     2881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     3061 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     3121 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     3181 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     3241 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     3301 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     3361 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     3421 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     3481 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     3541 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     3601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     3661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     3721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     3781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     3841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     3901 gcagctccag gcaagaatcc tggctgtgaa agatacctaa aggatcaaca gctcctgggg
     3961 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     4021 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     4081 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     4141 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     4201 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     4261 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     4321 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     4381 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     4441 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4501 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4561 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     4621 tggatcgata agcttgatat cgaattcggg aggtggtccc tgcagttacg ccaatgataa
     4681 cccccgccag aaaaatctta gtagccttcc ctttttgttt tccgtgcccc aactcggcgg
     4741 attgactcgg ccccttccgg aaacacccga atcaacttct agtcaaatta ttgttcacgc
     4801 cgcaatgacc cacccctggc ccgcgtctgt ggaactgacc cctggtgtac aggagagttc
     4861 gctgctgaaa gtggtcccaa aggggtacta gtttttaagc tcccaactcc ccctccccca
     4921 gcgtctggag gattccacac cctcgcaccg caggggcgag gaagtgggcg gagtccggtt
     4981 ttggcgccag ccgctgaggc tgccaagcag aaaagccacc gctgaggaga ctccggtcac
     5041 tgtcctcgcc ccgcctcccc cttccctccc cttggggacc accgggcgcc acgccgcgaa
     5101 cggtaagtgc cgcggtcgtc ggcgcctccg ccctccccct agggccccaa ttcccagcgg
     5161 gcgcggcgcg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     5221 ctgtaggcgc ggagtcctgc agccccgata aaataaaaga ttttatttag tctccagaaa
     5281 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     5341 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     5401 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     5461 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     5521 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     5581 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     5641 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     5701 ggcgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt
     5761 ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg cccgGCCACC atgaacaatt
     5821 cccagggaag agtgaccttc gaggatgtca ctgtgaactt cacccagggg gagtggcagc
     5881 ggctgaatcc cgaacagaga aacttgtaca gggatgtgat gctggagaat tacagcaacc
     5941 ttgtctctgt gggacaaggg gaaaccacca aacccgatgt gatcttgagg ttggaacaag
     6001 gaaaggagcc atggttggag gaagaggaag tgctgggaag tggccgtgca gaaaaaaatg
     6061 gggacattgg agggcagatt tggaagccaa aggatgtgaa agagagtctc tacccaactt
     6121 tcttgtacaa agtggttggt ggatctggag gttctcccaa gaagaagcgc aaggtgggac
     6181 gcgtctgcag gatatcaagc ttgcggtacc gcgggcccgg gatcgccacc atggacaaga
     6241 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
     6301 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     6361 agaagaacct gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc
     6421 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     6481 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     6541 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     6601 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     6661 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     6721 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     6781 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     6841 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     6901 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc
     6961 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac
     7021 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     7081 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg
     7141 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga
     7201 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc
     7261 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg
     7321 atggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg
     7381 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga
     7441 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc
     7501 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc
     7561 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct
     7621 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca
     7681 agggcgccag cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca
     7741 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc
     7801 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc
     7861 agaaaaaagc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc
     7921 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg
     7981 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca
     8041 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga
     8101 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg
     8161 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc
     8221 ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga
     8281 agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct
     8341 ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca
     8401 ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg
     8461 tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg
     8521 ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga
     8581 tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca
     8641 cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg
     8701 tggaccagga actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc
     8761 agagctttct gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc
     8821 ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc
     8881 gccagctgct gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg
     8941 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa
     9001 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
     9061 acgagaacga caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
     9121 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
     9181 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
     9241 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
     9301 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
     9361 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
     9421 agacaaacgg cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc
     9481 ggaaagtgct gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
     9541 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga
     9601 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc
     9661 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc
     9721 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag
     9781 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt
     9841 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa
     9901 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga
     9961 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac
    10021 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg
    10081 acgctaatct ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag
    10141 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct
    10201 tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg
    10261 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc
    10321 agctgggagg cgacgcctat ccctatgacg tgcccgatta tgccagcctg ggcagcggct
    10381 cccccaagaa aaaacgcaag gtggaagatc ctaagaaaaa gcggaaagtg aGCGGCCGCA
    10441 TAACTTCGTA TAAaGTATcC TATACGAAGT TATccGCTAC TAACTTCAGC CTGCTGAAGC
    10501 AGGCTGGAGA CGTGGAGGAG AACCCTGGAC CTgtgagcaa gggcgaggag ctgttcaccg
    10561 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
    10621 ctggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
    10681 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
    10741 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
    10801 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
    10861 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
    10921 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
    10981 tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
    11041 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
    11101 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
    11161 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
    11221 ctctcggcat ggacgagctg tacaagtaAT AACTTCGTAT AAaGTATcCT ATACGAAGTT
    11281 ATccaagtag tgaGCGATCG Ccctgcaggc atgcaagctt gatatcaagc ttatcgataa
    11341 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    11401 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    11461 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    11521 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    11581 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    11641 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    11701 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
    11761 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    11821 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    11881 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
    11941 acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
    12001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    12061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
    12121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    12181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    12241 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
    12301 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
    12361 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
    12421 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
    12481 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
    12541 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
    12601 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
    12661 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
    12721 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
    12781 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
    12841 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
    12901 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    12961 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
    13021 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
    13081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    13141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    13201 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    13261 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    13321 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    13381 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    13441 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    13501 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    13561 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    13621 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    13681 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    13741 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    13801 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    13861 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    13921 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    13981 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    14041 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    14101 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    14161 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    14221 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    14281 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    14341 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtctttcta gtgtagccgt
    14401 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    14461 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    14521 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    14581 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    14641 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    14701 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    14761 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    14821 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    14881 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    14941 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    15001 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt catta
//