LOCUS       pNM1125_-_pEF1a::Zim3- 15722 bp ds-DNA     circular     12-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        149..345
                     /label="SV40 early promoter"
     polyA_signal    complement(2058..2249)
                     /label="SV40 late polyA"
     repeat_region   4444..4623
                     /label="cPPT"
     misc_feature    4647..4887
                     /label="truncated UCOE"
     misc_feature    complement(4661..4669)
                     /label="CTCF binding site"
     misc_feature    4836..4849
                     /label="CTCF binding site"
     misc_feature    4888..5326
                     /label="truncated UCOE"
     promoter        5328..6505
                     /label="EF-1α promoter"
     intron          5559..6497
                     /label="EF-1α intron A"
     protein_bind    6560..6561
                     /label="attB1"
     misc_feature    6561..6860
                     /label="ZIM3 (1-100)"
     protein_bind    complement(6862..6886)
                     /label="attB2"
     CDS             6906..6926
                     /label="SV40 NLS"
     misc_feature    6975..6984
                     /label="vertebrate consensus sequence for strong initia"
     CDS             6981..11084
                     /label="dCas9"
     CDS             11088..11114
                     /label="HA"
     CDS             11133..11153
                     /label="SV40 NLS"
     CDS             11160..11180
                     /label="SV40 NLS"
     misc_feature    11188..11189
                     /label="to keep 2?"
     primer          11188..11207
                     /label="pJB108 pHR-UCOE-SFFV-ZIM3-dcas9-p2a-BFP FWD"
                     /note="sequence: aaagcggaaagtgaGCGGCCGCGCTACTAACTTCAGCCTG"
     misc_feature    11190..11246
                     /label="P2A"
     CDS             11247..11954
                     /label="enhanced monomeric blue fluorescent protein (Su"
     CDS             11247..11954
                     /label="Translation 11247-11954"
     primer          complement(11932..11957)
                     /label="oNM1570"
                     /note="sequence: ttgcatgcctgcaggGCGATcgcttaTTAattaagcttgtgccccagtttgc"
     misc_feature    11958..11963
                     /label="extra stop"
     misc_feature    11964..11968
                     /label="AsiSI"
     misc_feature    11969..11976
                     /label="SbfI"
     enhancer        12005..12596
                     /label="WPRE"
     primer_bind     complement(12963..12980)
                     /label="SP6"
     CDS             13873..14733
                     /label="bla (AmpR)"
     CDS             14071..14730
                     /label="AmpR"
ORIGIN
        1 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
       61 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      301 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
      361 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
      421 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
      481 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
      541 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
      601 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
      661 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
      721 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
      781 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
      841 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
      901 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
      961 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     1021 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     1081 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     1141 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     1201 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     1261 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     1321 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     1381 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     1441 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     1501 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     1561 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     1621 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     1681 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     1741 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     1801 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     1861 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     1921 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     1981 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     2041 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2101 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2221 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     2281 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     2341 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     2401 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     2461 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     2521 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     2581 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     2641 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     2701 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     2761 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     2821 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     2881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     3061 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     3121 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     3181 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     3241 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     3301 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     3361 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     3421 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     3481 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     3541 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     3601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     3661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     3721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     3781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     3841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     3901 gcagctccag gcaagaatcc tggctgtgaa agatacctaa aggatcaaca gctcctgggg
     3961 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     4021 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     4081 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     4141 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     4201 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     4261 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     4321 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     4381 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     4441 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4501 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4561 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     4621 tggatcgata agcttgatat cgaattcggg aggtggtccc tgcagttacg ccaatgataa
     4681 cccccgccag aaaaatctta gtagccttcc ctttttgttt tccgtgcccc aactcggcgg
     4741 attgactcgg ccccttccgg aaacacccga atcaacttct agtcaaatta ttgttcacgc
     4801 cgcaatgacc cacccctggc ccgcgtctgt ggaactgacc cctggtgtac aggagagttc
     4861 gctgctgaaa gtggtcccaa aggggtacta gtttttaagc tcccaactcc ccctccccca
     4921 gcgtctggag gattccacac cctcgcaccg caggggcgag gaagtgggcg gagtccggtt
     4981 ttggcgccag ccgctgaggc tgccaagcag aaaagccacc gctgaggaga ctccggtcac
     5041 tgtcctcgcc ccgcctcccc cttccctccc cttggggacc accgggcgcc acgccgcgaa
     5101 cggtaagtgc cgcggtcgtc ggcgcctccg ccctccccct agggccccaa ttcccagcgg
     5161 gcgcggcgcg cggcccctcc ccccgccggg cgcgcgcccg ctgccccgcc cttcgtggcc
     5221 gcccggcgtg ggcggtgcca cccctccccc cggcggcccc cctgcagccg cgcgcagctc
     5281 ccggctccct cccccttcgg atgtggcttg agctgtaggc gcggaggggc tccggtgccc
     5341 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     5401 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     5461 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     5521 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     5581 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     5641 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     5701 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     5761 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     5821 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     5881 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     5941 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     6001 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     6061 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     6121 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     6181 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     6241 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     6301 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     6361 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     6421 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     6481 tttttcttcc atttcaggtg tcgtggaatt ctcacgcgtc tgcaggatat caagcttgcg
     6541 gtaccgcggg cccgGCCACC atgaacaatt cccagggaag agtgaccttc gaggatgtca
     6601 ctgtgaactt cacccagggg gagtggcagc ggctgaatcc cgaacagaga aacttgtaca
     6661 gggatgtgat gctggagaat tacagcaacc ttgtctctgt gggacaaggg gaaaccacca
     6721 aacccgatgt gatcttgagg ttggaacaag gaaaggagcc atggttggag gaagaggaag
     6781 tgctgggaag tggccgtgca gaaaaaaatg gggacattgg agggcagatt tggaagccaa
     6841 aggatgtgaa agagagtctc tacccaactt tcttgtacaa agtggttggt ggatctggag
     6901 gttctcccaa gaagaagcgc aaggtgggac gcgtctgcag gatatcaagc ttgcggtacc
     6961 gcgggcccgg gatcgccacc atggacaaga agtacagcat cggcctggcc atcggcacca
     7021 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     7081 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggcgcc ctgctgttcg
     7141 acagcggaga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     7201 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     7261 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     7321 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     7381 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     7441 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     7501 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     7561 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     7621 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     7681 agaatggcct gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     7741 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     7801 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     7861 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     7921 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     7981 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     8041 agagcaagaa cggctacgcc ggctacatcg atggcggagc cagccaggaa gagttctaca
     8101 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     8161 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     8221 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     8281 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     8341 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     8401 ccccctggaa cttcgaggaa gtggtggaca agggcgccag cgcccagagc ttcatcgagc
     8461 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     8521 tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt gaaatacgtg accgagggaa
     8581 tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc catcgtggac ctgctgttca
     8641 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     8701 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     8761 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     8821 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     8881 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     8941 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     9001 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     9061 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     9121 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     9181 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     9241 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     9301 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     9361 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     9421 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     9481 ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgata
     9541 acaaagtgct gactcggagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     9601 aggtcgtgaa gaagatgaag aactactggc gccagctgct gaatgccaag ctgattaccc
     9661 agaggaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     9721 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     9781 tcctggactc ccggatgaac actaagtacg acgagaacga caaactgatc cgggaagtga
     9841 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     9901 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     9961 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
    10021 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
    10081 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
    10141 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt
    10201 gggataaggg ccgggacttt gccaccgtgc ggaaagtgct gtctatgccc caagtgaata
    10261 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
    10321 ggaacagcga caagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
    10381 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
    10441 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
    10501 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
    10561 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
    10621 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
    10681 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
    10741 agaaacagct gtttgtggaa cagcacaaac actacctgga cgagatcatc gagcagatca
    10801 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaggtg ctgagcgcct
    10861 acaacaagca cagagacaag cctatcagag agcaggccga gaatatcatc cacctgttta
    10921 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
    10981 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
    11041 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacgcctat ccctatgacg
    11101 tgcccgatta tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc
    11161 ctaagaaaaa gcggaaagtg aGCGGCCGCG CTACTAACTT CAGCCTGCTG AAGCAGGCTG
    11221 GAGACGTGGA GGAGAACCCT GGACCTgtgt ctaagggcga agagctgatt aaggagaaca
    11281 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
    11341 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
    11401 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
    11461 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
    11521 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
    11581 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
    11641 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
    11701 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
    11761 atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga
    11821 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
    11881 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
    11941 ggcacaagct taatTAAtaa gcgATCGCcc tgcaggcatg caagcttgat atcaagctta
    12001 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
    12061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
    12121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
    12181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
    12241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
    12301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
    12361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
    12421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
    12481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
    12541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
    12601 accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag
    12661 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    12721 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
    12781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    12841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    12901 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
    12961 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
    13021 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
    13081 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
    13141 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
    13201 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
    13261 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
    13321 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
    13381 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
    13441 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
    13501 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    13561 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    13621 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    13681 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    13741 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    13801 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    13861 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    13921 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    13981 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    14041 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    14101 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    14161 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    14221 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    14281 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    14341 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    14401 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    14461 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    14521 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    14581 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    14641 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    14701 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    14761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    14821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    14881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    14941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    15001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ctttctagtg
    15061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    15121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    15181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    15241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    15301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    15361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    15421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    15481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    15541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    15601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    15661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    15721 ta
//