LOCUS       Exported                7577 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   LG
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          106..5348
                     /host="Homo sapiens"
                     /strain="UNMC_8078"
                     /mol_type="genomic DNA"
                     /country="Nigeria"
                     /isolation_source="blood"
                     /collected_by="Stephen Obaro"
                     /collection_date=1-May-2013
                     /db_xref="taxon:573"
                     /organism="Klebsiella pneumoniae"
                     /submitter_seqid="UNMC-8078_NODE_9.ctg_1"
     gene            226..5328
                     /locus_tag="BME92_RS27120"
                     /old_locus_tag="BME92_27075"
                     /label=BME92_RS27120
     CDS             226..5328
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="BME92_RS27120"
                     /old_locus_tag="BME92_27075"
                     /product="hypothetical protein"
                     /label=DUF4297-STAND
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_087775949.1"
                     /translation="MNENLKKQSNPFSTGGGGVNFETRVQAAFALSILTGSSVPCLSQS
                     MRAKQIRFQNKYDGSNTDDFVIVAEDSDGQEGKLFAQIKHEITLSESPDSVFAEVIASA
                     WQDFKSDNFKMDHDVIVLITGPLQKTDIHNTLPILEWAKFALSAEDFMKKVQTKGFSSE
                     AKLKKIENFRLHLRHASNISEIDDTFLWSFLKSFHLISYDLDSSFSVVASLLCSQIQNN
                     SDEAPPLVLSKIITTIQNFNQNAGILSEENIPHDLKTLFNKVHKKNIKDDIRKLQERGT
                     YIYTGISNRICGIHINRNEQLAEIEKKCQESRFVFVTGIRGVGKSGIVKDFISTKYKDV
                     PFFYLRTEDLDKSHLNDVFTALGMSCSLAEIAGEFSLLKEKILIIESVEKVLELTYQNA
                     FIDLLHFIKNQNGWTVIATGRDYATQQLIFNYLQPSGIDYCNVEIKALEYQQLSEICHK
                     IPELEKLIENKRLTDLLKVPFFIDVAVRALKNGAQFHTEGTERDFREIIWSSVIAKETD
                     RRGGMPAKRKRTFIDIAKQRAKKMVVGILDDAFDPEIIAKLEEDNLIQRHERLNLISPS
                     HDVLEDWALDIFIDNEYVENFLVPNKFFEQIGDEPSINRAYRLWLSRNIKENKEINDFI
                     YKVLTDASIASYWKDETITAILQSDAVNIFLKMFRTQLLSNKNLLLIRVLFILRMTCQQ
                     PKTLYNQELEKEEQEISKTLYLQPYGICWDALINFIFENQNELVNSTSIHIIEMLNSWV
                     SILNISDNYPDISRKVGLLAIWLLEPLKDSYAREDEYKREKVISLLLKLSPTIVDEFDL
                     LMDKEVFIPDTSHRRLHYVEYLINQSLIGLNVPILCKYRPDFIERLARHEWLQDNIEDD
                     DSIFYKQTTGVDQYFGLNRESDFFPASGAKGPFKYLLHFHPRKALDFIIDLCNKTAKKY
                     STSEFATRSSGFMSDETLVKNVELTLNDGTVVTQFSSPHLWKGYRGHSTLPYILQCALM
                     ALENWLIDSLDSLEKNDTIEWLFNYILLSSNSVMPTSVLASVATGFPDKISKAAFPILR
                     CPEFYNLDIQRLVNERGGSEQNWFSNMFHNDIMNDTYVEERRIAALRPWRKETLESLLT
                     RLQLSGKNKADTLKIVDSLIALSLESNDENLRFLVHRADTRTWSFTADKENSRIIAQSN
                     PELPEDLQQIHDEHSATHTQNSLINKLFLWSRKSFEENINNTYFNSHTEALTAAKYLFD
                     KFQNNEVDNPVMVIGTITKTAAVCTRDLFENLSEDEKNWCKKIIIECIIMNADCINGLS
                     LHDKTDSYGSGACAFVLPKLFRYVKNDEELNELKFTLAIALTHENIDIGEYAAKGVREF
                     LWTTHPTLAFQCIYGATMFARFKKENINSQRFYLYSDTELAQAKAHWSYLITSFREEFF
                     NTTPPLIENISLKTHSSWHIHLPILMIPFCVDNPLLINLYKKIVHFTFDEEYKRHKSNH
                     DDETIQHDIRKQITNCFAEHFIHTRKTGFVPFKTLLLEGCSTAPRIVYMTKLLFHIAME
                     KAEDLESLWEFWLMLAPEAHKIALKDVNEQYSGIQSDLNQLLRGMLYADTRWQGHENEA
                     RDIAYGSVHILDFAKISGNNSHVFMALSSLIYHFHHIFFDEGIHILAEKLIMNSKLIDK
                     QINTAFYLEMAIGRYLQIENRGILSRKMYNSCMTLLSGIVETGSARAYYLRENLIRSRR
                     VFT"
     misc_feature    join(415..417,469..471,475..477)
                     /label=Conserved Cap4-like
     misc_feature    join(1180..1182,1189..1191,1195..1203)
                     /label=Walker A
     misc_feature    1369..1383
                     /label=Walker B
     terminator      5357..5404
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5624..6283)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(6284..6386)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6912..7457)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaggaca ctcaccgtag
      121 atggactctt ttaaaccatc ttatatgact ctgagacgtc tgttaaactt atagcgattc
      181 ggtctgccct ggttgcatgg catcaatata tggatggaag ccaagatgaa tgaaaatcta
      241 aaaaaacaga gcaatccatt ttcaacaggt ggtggtggcg ttaatttcga aactcgtgtg
      301 caagcagcgt ttgcattatc gattcttaca ggttcaagcg taccttgtct ctctcagagc
      361 atgagagcaa aacagataag gtttcagaac aaatatgatg gctcaaatac agatgatttt
      421 gtgatcgttg ctgaagacag cgatggtcag gaaggtaagt tatttgctca aattaaacat
      481 gagataacac tgagtgaatc cccagattcg gtttttgctg aagttatcgc cagtgcttgg
      541 caggacttca aaagcgacaa tttcaaaatg gatcacgatg ttattgtgtt gattactggt
      601 ccactccaga aaacagatat ccataataca cttccaatat tggaatgggc gaaatttgcc
      661 ttatctgcgg aagattttat gaagaaggta caaacaaaag ggttctcaag cgaagcaaag
      721 ttgaagaaaa tagaaaattt cagattgcat ttaagacacg caagcaatat aagtgaaatt
      781 gatgatactt ttctatggtc atttttgaag tcgtttcatt tgatttctta tgacttagat
      841 tctagttttt ctgttgtagc tagtttacta tgctcacaaa tccaaaataa ctcagatgag
      901 gctccccccc tagtattatc aaaaatcatc acaacaattc aaaattttaa tcagaatgcg
      961 ggaatactat ctgaagaaaa tatcccgcat gatttaaaaa cattatttaa taaagtacac
     1021 aaaaagaaca ttaaggatga catcaggaag cttcaagaac gtggtactta tatttataca
     1081 ggcatatcta atagaatttg tggcatacat attaaccgga acgaacaact tgctgaaatt
     1141 gaaaaaaaat gtcaggaaag tcgttttgtc tttgtcactg gtattcgagg tgttggaaaa
     1201 tcaggtattg taaaagattt tatttcaacc aaatataagg acgtcccttt tttctacttg
     1261 agaactgaag atttagacaa aagccatctc aatgatgtat ttactgcact tggcatgagt
     1321 tgtagtttag cagaaattgc tggtgagttt tctttattaa aagaaaagat tcttattatt
     1381 gagtcagtgg aaaaagtact ggaacttact taccaaaatg cttttattga cttattacat
     1441 ttcatcaaaa atcagaatgg gtggacagtt attgccacag ggcgagatta tgctacacag
     1501 caactaattt ttaattattt acaacccagt ggtattgatt attgcaacgt agagatcaaa
     1561 gcactggaat accagcaatt aagtgaaata tgtcacaaaa taccagaact ggaaaaatta
     1621 attgaaaata aaagactaac cgacttattg aaagtcccat tctttattga tgttgcggta
     1681 agagcattaa aaaatggtgc tcaatttcac accgaaggaa ctgagaggga cttcagagag
     1741 attatatggt catcagttat tgcgaaggaa acagatagaa gaggcgggat gccagccaaa
     1801 agaaagcgaa catttattga catagctaaa caacgcgcaa agaaaatggt cgtgggtatt
     1861 ctggatgacg cttttgaccc agaaatcatt gcaaagcttg aagaagataa tttaattcaa
     1921 cgacatgaga gactgaattt aatcagtcca agtcatgacg ttttagaaga ctgggcttta
     1981 gatatcttta ttgataatga atatgtagaa aatttcttag tacccaataa gtttttcgag
     2041 cagattggtg atgagccttc cattaatcgc gcctatagat tatggttaag cagaaacata
     2101 aaagaaaata aggagattaa tgattttatc tataaagtat taacggatgc ttctattgcc
     2161 agctattgga aagatgaaac tataactgcg attttacaaa gtgatgccgt taacattttt
     2221 ttaaaaatgt tcagaacaca acttttatcc aataaaaatt tactgcttat tagagtattg
     2281 tttatcttaa gaatgacatg ccaacagccg aaaacactgt ataaccaaga actagaaaaa
     2341 gaagaacaag aaatttctaa aacattatat ctacagcctt atggtatatg ctgggatgcc
     2401 ttgattaatt tcatttttga aaaccagaat gaacttgtga acagcacatc tattcatatt
     2461 atagaaatgt tgaattcatg ggtgtcaatt ttaaatatca gtgataatta tcctgatata
     2521 tccagaaaag taggtttatt ggcaatatgg ttacttgaac cactcaagga ttcgtatgct
     2581 cgcgaagatg aatataaaag agaaaaagtt atcagtttat tattaaaatt atcccctact
     2641 attgtagatg aatttgatct acttatggat aaagaagtct ttattcctga tacctcacac
     2701 cgccgattac attatgttga atatttaatt aatcaatcat taatcggctt gaatgttcct
     2761 atattatgca agtatcgacc agattttatt gagcgcctag ctcgtcacga atggttacaa
     2821 gataatattg aagatgatga cagcattttt tataaacaga ctacgggtgt agatcagtac
     2881 tttggactaa acagagaatc agattttttt cctgctagtg gtgctaaagg tccttttaaa
     2941 tatctattac attttcatcc tcgtaaagct ttagatttta taattgactt gtgcaataaa
     3001 acggcaaaaa aatattctac ttcagaattt gcaacccgct ctagtggctt tatgtctgat
     3061 gaaactttag tcaaaaacgt tgaacttaca ctcaatgacg gtaccgtcgt tacgcaattt
     3121 tcttctccac acttatggaa gggttataga ggccattcaa cgctaccata tatccttcaa
     3181 tgtgcattaa tggctttgga gaattggctc attgattcac tagatagcct tgaaaaaaat
     3241 gacactattg aatggctttt taattatatt ttactatcta gcaactcagt aatgccaaca
     3301 tctgtattgg catcagttgc tactggtttt ccagacaaaa ttagtaaagc tgcttttcct
     3361 atcctaagat gccctgagtt ttacaattta gatatccaac gtcttgtcaa tgaacgagga
     3421 ggaagtgaac aaaactggtt ctcgaatatg tttcataacg atatcatgaa tgacacttat
     3481 gtagaggaac gacgcattgc tgcacttcgt ccttggagaa aagaaactct agaaagttta
     3541 ttgactaggt tacagcttag cgggaaaaac aaggccgaca cactaaaaat tgtggatagc
     3601 ctaattgcgt tatccttaga gagcaacgat gagaacttac gttttctagt acacagagca
     3661 gatactagaa catggtcatt tactgctgat aaagagaaca gccggattat tgcgcaaagc
     3721 aatccggaat taccggagga tctgcaacaa attcatgatg aacatagtgc tacacatact
     3781 cagaacagtt taattaacaa attattctta tggtcaagaa aatcatttga agaaaatatt
     3841 aataatacat actttaattc tcatacagag gctcttaccg ctgcaaaata tctttttgat
     3901 aaatttcaaa acaatgaagt tgataacccg gttatggtaa ttggcacaat caccaaaacc
     3961 gcagcagttt gtacacgaga tttgtttgag aacctttccg aagatgaaaa aaactggtgt
     4021 aaaaaaataa ttattgaatg catcattatg aatgccgatt gtattaacgg gttatcactt
     4081 catgataaga cagatagtta tggttctggc gcttgcgcat ttgttttacc taaattattc
     4141 cgctacgtaa aaaatgatga agaactcaat gaattaaaat ttacattagc aatagcatta
     4201 acccatgaaa atattgatat cggtgaatat gctgcaaaag gggtaagaga gtttctatgg
     4261 actacccatc cgaccttggc cttccaatgc atatatggtg ctactatgtt tgcgaggttt
     4321 aaaaaagaaa atattaactc acaaagattt tatttatatt ctgatactga actagctcaa
     4381 gctaaagcgc attggtctta tttgataacc tcttttcgtg aagagttctt taacacaacc
     4441 ccaccattaa ttgaaaatat ttcattaaaa acacattcat cttggcacat tcaccttcca
     4501 atattgatga ttccattttg cgttgataat cctctgctaa ttaatttata caagaaaata
     4561 gtacatttta cgtttgatga ggaatacaag agacataaaa gtaatcatga cgatgaaacc
     4621 atccaacatg acattaggaa gcagatcacc aattgcttcg ctgagcattt tatacacaca
     4681 agaaaaactg gattcgtgcc atttaagaca ctgctacttg aaggatgttc aacggcacca
     4741 cgtattgtgt acatgactaa attactattc cacatcgcta tggagaaagc agaagaccta
     4801 gagtctttat gggaattctg gttaatgtta gcgccagaag cacacaaaat tgcgctaaaa
     4861 gatgtaaatg aacagtattc tggtatacag agtgatctga accaattgct gcgcggaatg
     4921 ttgtatgcag acactaggtg gcagggacac gagaatgaag cacgcgatat agcatatggt
     4981 tcagtgcata tactagattt tgcaaaaata tcaggaaata acagccacgt atttatggcg
     5041 ttatcatcgt tgatttatca ctttcatcat atatttttcg acgagggtat ccatatcctt
     5101 gctgaaaaac tcatcatgaa tagcaagtta attgataagc aaattaatac agcattttat
     5161 ctagaaatgg ctataggacg atatttgcaa attgaaaatc ggggaatatt gagtcgtaaa
     5221 atgtataatt cctgtatgac attattatcg ggtattgtcg agacaggttc tgcgagagcc
     5281 tattatctcc gggaaaatct aataagatca cgcagagtat ttacctaaat ggcttctttg
     5341 taatggatcg caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     5401 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     5461 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     5521 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     5581 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     5641 actcatcgca atactgttgt aattcattta acattctgcc gacatggaag ccatcacaga
     5701 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     5761 tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     5821 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     5881 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     5941 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     6001 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcatcgcca
     6061 tacggaactc tggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     6121 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     6181 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     6241 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     6301 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     6361 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
     6421 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
     6481 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
     6541 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     6601 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     6661 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     6721 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     6781 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     6841 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     6901 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     6961 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     7021 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     7081 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     7141 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     7201 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     7261 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     7321 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     7381 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     7441 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     7501 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat
     7561 tctcatgtta gtcatgc
//
