LOCUS       Exported                7121 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   LG
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             232..4818
                     /codon_start=1
                     /label=Avs4
                     /protein_id="MBE0492665.1"
                     /translation="MNEFIKFLKNQYKNFFTKIDDTFDKFQNTAIGKFVIEKLILRAKS
                     FYSILSTFGLILVAQISAYNNIMQWSNKVDEQLNNSDGWAYAGWFIVSFIVPSGNMAVI
                     VALALILIVASIIRDKELSQSNTIERFIHSLEEKTKNILFEIRTNISYRNEKIELDRDE
                     IIEHIKNELEEKQIFIISGSGGVGKTSIIKNLYVNKEGNYPFYIFKAREFKTNDFLFNH
                     SIQDFIESNKQHTKKIIVIDSAEALLDFESDLFREFISALIQNNWKIIFTTRYSYLDDL
                     NYHFTQILNIVPFRIDIKNLSSEYLEALSSRYKFILPQDEKLLDLIKNPFYLNEYLANY
                     SNEEINYQDFKNKLWNQHISTPDIEKCFFEFAFKRANEGQFYVDIDCSSNSLKQLQNKG
                     ILGYEKSLGYFITHDIYEEWALEKIVNKEFKNKVDIKSFFEKIGSSLPIRRSFRSWVSE
                     KLLLDDENIKTFIEEIIDSVDIEIFWKDEIFISILLSEYSDTFFTNFKKELLENDYELL
                     SRISFLLRLACKEVDNSFWESLGLKNQQTKEAKYIFTKPKGTGWKSFINFIYENIETIG
                     LAKIDLVLPILQDWNSKFQERSTTRNASLTALKYYDLINQKEHKYSYKESIKTICKVIT
                     SGSSEIKDELSTIFDEIVEQKLKNHSDNYYELSKVVLTSWDGLLISKNLPKQVLKLADL
                     FWTKTPRKVKNDGIFPHYEREEVEDAFNLSSKYENKYFPASALQTPIYFLLKNHFSLTL
                     DFILDLINKSVEYYAKSGWEYKEEIQIVDVFIDENITIQQYHSQSLWNIYRGNSSPVMP
                     NLIQSIHMALEKYLLEIGKVLKTEDLEFWLLYLLQKSKSSSISAVVTSIVLANSDKAFN
                     IARILFKTKKFIQADFHRHIQEQSLKSLYGMGYGLNWQTKIYQDERLKTCEDKHRNSNL
                     ENLFLNYQFFRTSEVSEEDAKNIQDTLWGILDNYYKQLPDEESQSEEDRIWRIALARID
                     RRKMNIETEKVDNGIQITFNPKLSPELQKYSQEAQNNSQNTIKYTSLYLWAVNKIENNQ
                     DYKKYTNYEENPLLALEQIKEVIAIPHDQRDFIFQDEIFPNASIVLLRDYAEMLSSEDK
                     EICRDMILKFARLPLSENYHYQISDGVDKAIKYLPMLLIYFPELKNDIKILLLLNLFCD
                     YQIGMSGKHFYDFAIEAINSYFNSESNSFIAIYLLLKPKYNELITSNYYGQKGVSKQDL
                     LENFFQSNENLLENFVSNKIVEFDIGTIETNKLKNLIVVFKMISLNTTAKKEKELVRKI
                     IEIFCVKIFNQPDEKVSYDLKLKFINRFALFVLEADKSDIQSYLQPLLNQFEPTEDIVY
                     LLTQFILAQDEINEYDNFWHVWELFEDTIIETCKDGDKYRHIDQVIKVYLLAWGQYGAI
                     WKDTAKEWHSLKDKDIRYFKKLTKKIGHCPSVLYSISKLLTSIASKYQNDGISWVADLL
                     RNNSNLSNDKLEPDTVFHLENLIRQYVFIQGEQIKKDRKIKDDVLTILNFLVEKGSAVA
                     YMLREKVL"
     misc_feature    367..435
                     /label=Transmembrane
     misc_feature    478..582
                     /label=Transmembrane
     misc_feature    join(775..777,790..798)
                     /label=Walker A
     misc_feature    943..957
                     /label=Walker B
     terminator      4901..4948
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5168..5827)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5828..5930)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6456..7001)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagcgca gctgacaaag
      121 attgaccgtg agcgctctga tggagaaaga cgatagttgc tgagtacgat atcgagggta
      181 catttctctg tgtaggggta gttatttaca aaaaaatagg agaataatta aatgaacgag
      241 ttcatcaagt ttctgaaaaa ccagtacaag aacttcttta ccaagatcga cgataccttc
      301 gataagttcc aaaacaccgc gatcggcaag ttcgtgatcg aaaaactgat tctgcgtgcg
      361 aagagcttct atagcattct gagcaccttt ggtctgattc tggtggcgca gatcagcgcg
      421 tacaacaaca tcatgcagtg gagcaacaaa gttgacgagc aactgaacaa cagcgatggt
      481 tgggcgtacg cgggctggtt catcgttagc tttattgtgc cgagcggtaa catggcggtg
      541 atcgttgcgc tggcgctgat cctgattgtt gcgagcatca ttcgtgacaa agagctgagc
      601 cagagcaaca ccatcgaacg tttcattcac agcctggagg aaaagaccaa aaacatcctg
      661 tttgaaattc gcaccaacat tagctatcgc aatgagaaga tagagctgga tcgcgatgaa
      721 attattgaac atattaaaaa cgaactggaa gaaaaacaga tttttattat tagcggcagc
      781 ggcggcgtgg gcaaaacgag cattatcaaa aacttgtacg tcaacaaaga aggtaattat
      841 ccgttttata tttttaaagc gcgcgaattt aaaaccaacg attttctgtt taaccatagc
      901 attcaagatt ttattgaaag caacaaacag cataccaaga aaataattgt tattgatagc
      961 gcggaagcgc tgctggattt tgaaagcgat ctgtttcgcg aatttattag cgcgctgatt
     1021 cagaacaact ggaaaattat ttttaccacc cgctatagct atctggatga tctgaattac
     1081 cacttcacgc agattctgaa cattgtgccg tttcgcattg atataaaaaa tctgagcagt
     1141 gaatatctgg aagcgctgag cagccgctat aaatttattc tgccccaaga tgaaaagctg
     1201 ttggatctta tcaaaaaccc gttttatctg aacgaatatc tggcgaacta tagcaatgaa
     1261 gagattaatt atcaagattt caaaaacaag ttatggaatc agcatattag caccccggat
     1321 attgaaaaat gcttttttga atttgcgttt aaacgcgcga acgaaggtca gttttatgtg
     1381 gatattgatt gcagcagcaa cagcctgaaa cagctgcaga acaaaggcat tctgggctat
     1441 gaaaaaagcc tgggctattt tattacccat gatatttatg aagagtgggc gcttgaaaaa
     1501 attgtaaata aagaatttaa aaataaagtg gacattaaaa gcttttttga aaaaataggc
     1561 agcagcctgc cgattcgccg cagctttcgc agctgggtga gcgagaaatt gctattagat
     1621 gatgagaaca tcaagacctt tattgaagaa atcattgata gcgtggacat tgagattttt
     1681 tggaaagatg aaattttcat tagtattctg ctgagcgaat atagcgatac cttttttaca
     1741 aactttaaaa aagaactgct ggaaaatgat tatgaactgc tgagccgcat tagctttctg
     1801 ctgcgcctgg cgtgcaaaga agtggataac agcttttggg aaagcctggg cctgaagaat
     1861 cagcagacca aagaagcgaa atatattttt accaaaccga aaggcaccgg ctggaaaagc
     1921 tttattaact ttatttatga aaatattgaa accattggcc tggcgaaaat tgatctggtg
     1981 ctgccgattc tgcaagattg gaacagcaaa tttcaagaac gcagcaccac ccgcaacgcg
     2041 agcctgaccg cgctcaagta ctatgacttg attaatcaga aggagcataa atatagctac
     2101 aaagaaagca ttaaaaccat ttgtaaggtg attacgagcg gcagcagcga gattaaagac
     2161 gaactcagca ccatctttga tgaaatagtg gagcagaaac tcaaaaacca ctctgataac
     2221 tattacgagc tgagcaaagt ggtgctgacg agctgggatg gcctgctgat tagcaaaaac
     2281 ctgccgaaac aagtgctgaa actggcggat ctgttttgga ccaaaacccc gcgcaaagtg
     2341 aaaaacgacg gcatttttcc gcattatgaa cgcgaagaag tggaagatgc gtttaactta
     2401 agctcgaaat atgagaacaa gtacttcccg gcgagcgcgc tgcagacccc gatttatttt
     2461 ctgctgaaaa atcacttttc cctgaccctg gattttattc tggatcttat taacaaaagc
     2521 gtggaatatt atgcgaaaag cggctgggaa tataaagaag aaattcagat tgtggatgtg
     2581 tttattgatg aaaacattac cattcagcag tatcatagtc agagcctgtg gaacatttat
     2641 cgcggcaaca gcagcccggt gatgccgaac ctgattcaga gcattcatat ggctctggaa
     2701 aagtatttac tggaaattgg caaagtgctg aaaaccgaag atctggaatt ttggctgctg
     2761 tatctgctgc agaaaagcaa aagcagcagc attagcgcgg tggtcacgag catagtgctg
     2821 gcgaacagcg ataaagcgtt taacattgcg cgcattctgt ttaaaacaaa gaagtttatt
     2881 caagcggatt ttcatcgcca tattcaagaa cagagcctga aaagcctgta tggcatgggc
     2941 tatggcctga actggcagac caaaatttat caagatgaac gcctgaaaac ctgcgaagat
     3001 aaacatcgca acagcaatct ggagaacctg tttctaaact atcagttctt tcgcacgagc
     3061 gaagtgagcg aagaagacgc gaaaaacatt caagataccc tgtggggcat tctggataac
     3121 tactacaaac aactgccgga tgaagaaagt cagagcgagg aagataggat ttggcgcatt
     3181 gcgctggcgc gcattgatcg ccgcaaaatg aacattgaga ccgaaaaagt ggataacggc
     3241 attcagatta cctttaaccc gaaactgagc ccggaactgc agaaatatag ccaagaagcg
     3301 cagaacaaca gtcagaacac cattaaatat acgagcctgt atctgtgggc ggtgaacaaa
     3361 attgaaaaca accaagatta taaaaaatat accaactatg aagaaaaccc gctgctggcg
     3421 ctggaacaga tcaaagaagt gattgcgatt ccgcatgatc agcgcgattt tatttttcaa
     3481 gatgaaatat ttcctaatgc gtccattgtc ctgctgcgcg attatgcgga aatgctgagt
     3541 agcgaagata aagaaatttg ccgcgatatg attctgaaat ttgcgcgcct gccgctgagc
     3601 gaaaactatc attatcagat tagcgatggc gtggataaag cgattaaata tctgccgatg
     3661 ctgctgattt attttccgga actgaaaaac gatattaaaa ttctgctgct gctgaacctg
     3721 ttttgcgatt atcagattgg catgagcggc aaacattttt atgattttgc gattgaagcg
     3781 attaacagct attttaacag cgaaagcaac agctttattg cgatttatct gctgctgaaa
     3841 ccgaaatata acgaactgat tacgagcaac tattatggtc agaaaggcgt gagcaaacaa
     3901 gatctactcg agaacttttt tcagagcaac gaaaaccttc tggaaaattt cgttagcaac
     3961 aaaattgtgg aatttgatat tggcaccatt gaaaccaaca aactgaaaaa tctgatagtg
     4021 gtgtttaaaa tgattagcct gaacaccacc gcgaaaaaag aaaaagaact ggtgcgcaaa
     4081 attatagaaa ttttttgcgt gaaaattttt aatcagccgg atgaaaaagt gagctatgat
     4141 ctgaaactga aatttattaa ccgctttgcg ctgtttgtgc tggaagcgga taaaagcgat
     4201 attcagagct atctgcagcc gctgctgaat cagtttgaac cgaccgaaga tattgtgtat
     4261 ctgcttacgc agtttattct ggcgcaagat gaaattaacg aatatgataa cttttggcat
     4321 gtgtgggaac tgtttgaaga taccattatt gaaacctgca aagatggcga taaatatcgc
     4381 catattgatc aagtgattaa agtgtatctg ctggcgtggg gtcagtatgg cgcgatttgg
     4441 aaagataccg cgaaagaatg gcatagcctg aaagataaag atattcgcta ttttaaaaaa
     4501 ctgaccaaaa agatcggcca ctgcccgagc gtgctgtata gcattagcaa actgctgact
     4561 agcattgcga gcaaatatca gaatgacggc atctcatggg tggcggatct gctgcgcaac
     4621 aacagcaacc tgagcaacga taaactggaa ccggataccg tgtttcatct ggaaaacctc
     4681 atccggcagt atgtgtttat tcaaggcgag caaataaaga aagaccgcaa gattaaagat
     4741 gatgtgctga ccattctgaa ctttctggtg gaaaaaggca gcgcggtggc gtatatgctg
     4801 cgcgaaaaag tgctgtaatg tagttgaaaa taataatttt aatgagagct tttccaattt
     4861 aggctccagg gattggagcc tttttattat cgcgcaataa ctagcataac cccttggggc
     4921 ctctaaacgg gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt
     4981 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     5041 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     5101 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
     5161 aaaaaaatta cgccccgccc tgccactcat cgcaatactg ttgtaattca tttaacattc
     5221 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     5281 ccttgtcgcc ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca
     5341 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     5401 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     5461 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     5521 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     5581 ccagctcacc gtctttcatc gccatacgga actctggatg agcattcatc aggcgggcaa
     5641 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     5701 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     5761 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     5821 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     5881 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     5941 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     6001 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     6061 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     6121 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     6181 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     6241 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     6301 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     6361 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     6421 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     6481 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     6541 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     6601 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     6661 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     6721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     6781 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     6841 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     6901 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     6961 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     7021 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     7081 gccccatacg atataagttg taattctcat gttagtcatg c
//
