LOCUS       Exported                9738 bp DNA     circular SYN 12-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   LG
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             76..80
     CDS             88..6363
                     /codon_start=1
                     /label=SeAvs3
                     /protein_id="WP_126523998.1"
                     /translation="MSDSLLVRTSRDGDQFHYLWAARRALRLLEPQSTLVALTIEGAST
                     TEMGSQPVVEDGEELIDIAEYYGSNELATATTVRYMQLKHSTMHSDTPFPPSGLQKTIE
                     GFATRYKALIQKIPVETLRTKLEFWFVTNRPVSSSFSEAINDAANQHVTRHPHDLAKLE
                     KFTGLQGAELSIFCQLLHIEGQQDDLWSQRNILLRESAGYLPDLDTEAPLKLKELVNRK
                     ALTESAANPSITRMDVLRALGVDETDLFPAPCRIERIENSVSRTQEATLVQRVVEAFGA
                     PVIIHADAGVGKSIFSTHIEEHLPTGSVSILYDCFGLGQYRNASSYRHHHRTALVQMAN
                     EMASRGLCHPLIPNAGTGISQYMRAFLHRLSQSISILRASEPLAVLCIIIDAADNAQMA
                     AEEIGETRSFIKDLIREKLPDGVCLVALCRPYRRELLDPPPEALTLSLQTFNRDETAAH
                     LHQKFPDASESDVDEFHRLSSCNPRVQALSLSQNLPLNDTLRLLGPNPKTVEDTIGEVL
                     EKSIARLRDTAGISERAQIDTICSALAILRPLIPLSVLSAISGVAGSAIKSFALDLGRP
                     LIVSGETIQFFDEPAETWFQRRFRPSAADLHQFITKLRPLTKDSSYAASVLPALMLEGN
                     QLSELIELAISSQALPETSAVERRDIELQRLQFALKAALRTGRYQDAAKLALKAGGECA
                     GDNRQRVLLRDNIDLAAKFVGSNGVQELVSRNAFPDTGWPGSRNAYYAAILSEYPELSG
                     EARSRLRLTMEWLTNWSQLPDDERSRQNVTDQDRAVMLIACLNIHGAEAAARELRRWRP
                     RKLSFDAGKIVAMQLLAHARYDELDQLAIAAGNDISLVMGIVLEARKLHRPVAEQAIRR
                     TWRLLKSQRVSIKDRNHANNQTIAAITGMVEMALIQSVCTESESIQLLDRYLPKVPPYA
                     LTSEYSKERVAYVRAYALQANLMGSQLALSDLASTEVKKELMAEKRHGESDDLRQLKQY
                     SGVLIPWYNLWAKVILGKTRKADLESELSDTQKESTAIKGHSYSEHSLSSNEIANVWFD
                     ILIEAGNVSKDDVENIIKWSQHKGNRVFTPTLHRFSSVCAEISGLGELSYHFAELALSL
                     WRDEHSDAQIKADGYIDLSRSLISLDEPEAKEYFNQAIEVTNKLGDENLSRWEAILDLA
                     EYVAGKTQVPPEISYKLARCAELTREYVDRDKHFAWSDTVEILAELCPSSALAIISRWR
                     DRTFGNHRSILAWTIEHLVKKNKINALDALPLITFENDWHKCDLLDSVLSSCTDDKDKI
                     MAFEVVYHYTKFNVQNIQNLKKLDAISTSLGIEHTELKERISGLQHTETVSKKSSLSSN
                     DNEQGHDQEWESIFKDCDLSSIDGISAAYEKFRNVPEFYSKETFIKKAISRVKTGKECS
                     FITAIGAIFHWGLYDFKYILESIPDEWTSRLSIKTTLAGLIKEYCQRFCMRIRKSRVYE
                     IFPFSLASRLSGISEKEIFGITLEAIAESPEPANSDRLFSLPGLLVSKLESNEALDVLS
                     YALDLFDEVLKDEDGDGPWNEKLSPPTHVEDSLAGYIWARLGSPEAEMRWQAAHAVLAL
                     CRMSRTCVIQGIFQHAINATTLPFCDRNLPFYTLHAQLWLMIAAARVALDDGKSLIPNI
                     GYFYHYATTDQPHVLIRHFAARTLLALHDSDLISIPAQEENKLRNINQSTTLPVLDKVE
                     DHRGEDSYTFGIDFGPYWLKPLGRCFGVSQKQLEPEMLRIIRDVLGFKGSRNWDEDERN
                     KRRYYQDRDNHHSHGSYPRVDDYHFYLSYHAMFMTAGQLLATKPLVGSDYDDVEDVFQD
                     WLRRHDISRNDHRWLADRRDIPPKERSSWLNSSSDNRDEWLASISENVFNETLCPSPGL
                     LTLWGRWSDVCSDRKESIIVHSALVSPERSLSLLRALQTTKNVYDYKIPDAGDNLEIDH
                     AHYQLKGWIKDIAEYCGIDEFDPWAGNVRFPIPEPASFIIDAMKLTTDKDHRVWYSPSD
                     VEPAMISSIWGHLSGKNDEEKSHGYRLCASIHFIKSALETFNMDLILEVDVDRYSRNSR
                     YERNNENELDNIPSSTRLFLFRHDGTIHTLYGNYRNGEKTS"
     CDS             6373..6390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6436..6483
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6654..7445)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7446..7537)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7585..8323)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(8533..9615)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(9616..9693)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taataaggag atataccatg agcgacagcc tgctggttcg taccagccgt
      121 gacggtgatc agttccacta cctgtgggcg gcgcgtcgtg cgctgcgtct gctggaaccg
      181 caaagcaccc tggtggcgct gaccatcgaa ggtgcgagca ccaccgagat gggtagccag
      241 ccggtggttg aggatggcga ggaactgatt gacatcgcgg aatactatgg tagcaacgag
      301 ctggcgaccg cgaccaccgt tcgttacatg caactgaagc acagcaccat gcatagcgac
      361 accccgtttc cgccgagcgg tctgcagaaa accattgaag gcttcgcgac ccgctataag
      421 gcgctgattc aaaaaatccc ggttgaaacc ctgcgtacca agctggagtt ctggtttgtg
      481 accaaccgtc cggttagcag cagctttagc gaggcgatca acgatgcggc gaaccaacat
      541 gtgacccgtc acccgcacga cctggcgaag ctggaaaaat ttaccggtct gcagggcgcg
      601 gagctgagca ttttctgcca actgctgcac atcgagggtc agcaagacga tctgtggagc
      661 caacgtaaca ttctgctgcg tgagagcgcg ggctacctgc cggacctgga taccgaagcg
      721 ccgctgaagc tgaaagagct ggtgaaccgt aaagcgctga ccgaaagcgc ggcgaacccg
      781 agcatcaccc gtatggacgt gctgcgtgcg ctgggtgttg atgagaccga cctgttcccg
      841 gcgccgtgcc gtattgagcg tatcgaaaac agcgtgagcc gtacccagga agcgaccctg
      901 gttcaacgtg tggttgaggc gtttggtgcg ccggtgatca ttcacgcgga cgcgggtgtt
      961 ggcaaaagca tcttcagcac ccacattgag gaacacctgc cgaccggcag cgttagcatc
     1021 ctgtacgatt gctttggtct gggccagtac cgtaacgcga gcagctatcg tcaccaccac
     1081 cgtaccgcgc tggtgcaaat ggcgaacgaa atggcgagcc gtggtctgtg ccacccgctg
     1141 attccgaacg cgggtaccgg catcagccag tatatgcgtg cgttcctgca ccgtctgagc
     1201 caaagcatta gcattctgcg tgcgagcgag ccgctggcgg ttctgtgcat cattatcgat
     1261 gcggcggaca acgcgcagat ggcggcggag gaaatcggtg aaacccgtag ctttatcaag
     1321 gatctgattc gtgagaaact gccggatggt gtgtgcctgg ttgcgctgtg ccgtccgtac
     1381 cgtcgtgaac tgctggaccc gccgccggag gcgctgaccc tgagcctgca gaccttcaac
     1441 cgtgatgaga ccgcggcgca cctgcaccaa aagtttccgg acgcgagcga gagcgacgtt
     1501 gatgaattcc accgtctgag cagctgcaac ccgcgtgtgc aggcgctgag cctgagccaa
     1561 aacctgccgc tgaacgatac cctgcgtctg ctgggtccga acccgaagac cgtggaagac
     1621 accattggcg aggttctgga gaagagcatc gcgcgtctgc gtgataccgc gggtatcagc
     1681 gaacgtgcgc agattgacac catctgcagc gcgctggcga tcctgcgtcc gctgattccg
     1741 ctgagcgtgc tgagcgcgat tagcggtgtt gcgggcagcg cgatcaaaag ctttgcgctg
     1801 gatctgggtc gtccgctgat cgtgagcggc gaaaccattc agttctttga cgagccggcg
     1861 gaaacctggt tccaacgtcg ttttcgtccg agcgcggcgg atctgcacca gttcatcacc
     1921 aagctgcgtc cgctgaccaa agacagcagc tatgcggcga gcgttctgcc ggcgctgatg
     1981 ctggagggta accagctgag cgagctgatc gaactggcga ttagcagcca ggcgctgccg
     2041 gagaccagcg cggtggaacg tcgtgacatc gagctgcagc gtctgcaatt tgcgctgaag
     2101 gcggcgctgc gtaccggtcg ttatcaggat gcggcgaagc tggcgctgaa ggcgggtggc
     2161 gaatgcgcgg gtgacaaccg tcaacgtgtt ctgctgcgtg ataacattga cctggcggcg
     2221 aaatttgtgg gtagcaacgg cgtgcaggag ctggttagcc gtaacgcgtt cccggatacc
     2281 ggttggccgg gtagccgtaa cgcgtactat gcggcgatcc tgagcgagta cccggaactg
     2341 agcggtgaag cgcgtagccg tctgcgtctg accatggagt ggctgaccaa ctggagccaa
     2401 ctgccggacg atgaacgtag ccgtcagaac gtgaccgacc aagatcgtgc ggttatgctg
     2461 atcgcgtgcc tgaacattca tggtgcggaa gcggcggcgc gtgagctgcg tcgttggcgt
     2521 ccgcgtaagc tgagcttcga cgcgggcaaa attgttgcga tgcagctgct ggcgcatgcg
     2581 cgttacgatg agctggacca actggcgatc gcggcgggta acgatattag cctggtgatg
     2641 ggcatcgttc tggaagcgcg taaactgcac cgtccggttg cggagcaggc gattcgtcgt
     2701 acctggcgtc tgctgaagag ccaacgtgtg agcatcaaag accgtaacca cgcgaacaac
     2761 cagaccattg cggcgatcac cggtatggtg gagatggcgc tgattcagag cgtttgcacc
     2821 gagagcgaaa gcatccaact gctggatcgt tacctgccga aggttccgcc gtacgcgctg
     2881 accagcgaat atagcaaaga gcgtgtggcg tacgttcgtg cgtatgcgct gcaggcgaac
     2941 ctgatgggta gccaactggc gctgagcgac ctggcgagca ccgaagtgaa gaaagagctg
     3001 atggcggaaa agcgtcacgg tgaaagcgac gatctgcgtc agctgaaaca atacagcggc
     3061 gtgctgattc cgtggtataa cctgtgggcg aaggttatcc tgggcaagac ccgtaaagcg
     3121 gatctggaga gcgaactgag cgacacccag aaggagagca ccgcgattaa aggccacagc
     3181 tacagcgaac acagcctgag cagcaacgag atcgcgaacg tttggttcga cattctgatc
     3241 gaagcgggta acgtgagcaa agacgatgtt gagaacatta tcaagtggag ccaacacaaa
     3301 ggcaaccgtg tgtttacccc gaccctgcac cgtttcagca gcgtttgcgc ggaaatcagc
     3361 ggtctgggcg agctgagcta tcactttgcg gaactggcgc tgagcctgtg gcgtgatgag
     3421 cacagcgacg cgcagattaa ggcggatggt tacatcgacc tgagccgtag cctgattagc
     3481 ctggacgagc cggaagcgaa ggaatatttc aaccaagcga tcgaggtgac caacaaactg
     3541 ggtgatgaaa acctgagccg ttgggaggcg attctggacc tggcggaata tgtggcgggc
     3601 aagacccagg ttccgccgga gatcagctac aaactggcgc gttgcgcgga gctgacccgt
     3661 gaatatgttg accgtgataa acactttgcg tggagcgaca ccgtggagat cctggcggaa
     3721 ctgtgcccga gcagcgcgct ggcgattatc agccgttggc gtgatcgtac cttcggtaac
     3781 caccgtagca tcctggcgtg gaccattgaa cacctggtta agaaaaacaa gattaacgcg
     3841 ctggatgcgc tgccgctgat caccttcgag aacgactggc acaaatgcga tctgctggac
     3901 agcgttctga gcagctgcac cgacgataag gacaaaatta tggcgtttga ggtggtttac
     3961 cactatacca agttcaacgt gcagaacatc caaaacctga agaaactgga tgcgattagc
     4021 accagcctgg gtatcgagca caccgaactg aaagagcgta ttagcggcct gcagcacacc
     4081 gaaaccgtga gcaagaaaag cagcctgagc agcaatgaca acgagcaggg tcacgaccaa
     4141 gagtgggaaa gcatctttaa ggactgcgat ctgagcagca ttgacggcat cagcgcggcg
     4201 tacgaaaagt tccgtaacgt tccggaattt tatagcaaag agaccttcat caagaaagcg
     4261 attagccgtg tgaagaccgg taaagagtgc agcttcatta ccgcgatcgg tgcgattttt
     4321 cactggggcc tgtacgattt caaatatatc ctggaaagca ttccggacga gtggaccagc
     4381 cgtctgagca ttaagaccac cctggcgggc ctgatcaaag aatactgcca gcgtttctgc
     4441 atgcgtattc gtaagagccg tgtttatgag atcttcccgt ttagcctggc gagccgtctg
     4501 agcggtatta gcgagaaaga aatctttggc attaccctgg aggcgattgc ggaaagcccg
     4561 gagccggcga acagcgaccg tctgttcagc ctgccgggtc tgctggttag caagctggaa
     4621 agcaacgagg cgctggatgt tctgagctac gcgctggacc tgttcgacga agtgctgaag
     4681 gacgaggatg gtgacggccc gtggaacgaa aaactgagcc cgccgaccca tgtggaggac
     4741 agcctggcgg gttatatttg ggcgcgtctg ggtagcccgg aagcggaaat gcgttggcaa
     4801 gcggcgcatg cggttctggc gctgtgccgt atgagccgta cctgcgtgat ccagggcatt
     4861 ttccaacacg cgattaacgc gaccaccctg ccgttttgcg accgtaacct gccgttctac
     4921 accctgcatg cgcagctgtg gctgatgatt gcggcggcgc gtgttgcgct ggatgatggt
     4981 aaaagcctga tcccgaacat tggctacttt tatcactacg cgaccaccga tcaaccgcac
     5041 gtgctgattc gtcactttgc ggcgcgtacc ctgctggcgc tgcacgatag cgacctgatt
     5101 agcatcccgg cgcaggaaga gaacaagctg cgtaacatta accaaagcac caccctgccg
     5161 gtgctggata aagttgaaga ccaccgtggt gaggatagct acaccttcgg tatcgacttt
     5221 ggcccgtatt ggctgaagcc gctgggtcgt tgctttggcg ttagccagaa acaactggag
     5281 ccggaaatgc tgcgtattat ccgtgacgtg ctgggtttca agggcagccg taactgggat
     5341 gaggacgaac gtaacaaacg tcgttactat caggatcgtg acaaccacca cagccacggc
     5401 agctacccgc gtgttgacga ttatcacttt tacctgagct atcacgcgat gttcatgacc
     5461 gcgggtcagc tgctggcgac caagccgctg gttggtagcg attatgacga tgtggaagat
     5521 gtttttcaag actggctgcg tcgtcacgat attagccgta acgaccaccg ttggctggcg
     5581 gatcgtcgtg acatcccgcc gaaagaacgt agcagctggc tgaacagcag cagcgataac
     5641 cgtgacgagt ggctggcgag catcagcgaa aacgttttca acgagaccct gtgcccgagc
     5701 ccgggtctgc tgaccctgtg gggccgttgg agcgatgtgt gcagcgaccg taaggaaagc
     5761 attattgtgc acagcgcgct ggttagcccg gagcgtagcc tgagcctgct gcgtgcgctg
     5821 cagaccacca agaacgttta tgactacaaa attccggatg cgggtgacaa cctggagatc
     5881 gatcacgcgc actaccaact gaagggctgg atcaaagaca ttgcggaata ttgcggtatt
     5941 gatgagttcg acccgtgggc gggcaacgtg cgttttccga tcccggaacc ggcgagcttc
     6001 attatcgatg cgatgaagct gaccaccgat aaagaccatc gtgtgtggta cagcccgagc
     6061 gacgttgagc cggcgatgat cagcagcatt tggggtcacc tgagcggcaa gaacgatgag
     6121 gagaagagcc acggttatcg tctgtgcgcg agcatccact ttattaagag cgcgctggaa
     6181 accttcaaca tggacctgat cctggaagtg gatgttgacc gttacagccg taacagccgt
     6241 tatgaacgta acaacgagaa cgaactggat aacattccga gcagcacccg tctgttcctg
     6301 tttcgtcacg acggcaccat ccacaccctg tacggtaact atcgtaacgg cgagaaaacc
     6361 agcggcggaa gccatcacca tcatcaccac agcagcggct aattaaccta ggctgctgcc
     6421 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     6481 ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
     6541 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
     6601 gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag acattatttg
     6661 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     6721 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     6781 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     6841 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     6901 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     6961 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     7021 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     7081 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     7141 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     7201 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     7261 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     7321 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     7381 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     7441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact cggtcgctac
     7561 gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc
     7621 gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt
     7681 tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc ggtgcatctg
     7741 tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa
     7801 agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg
     7861 tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct
     7921 cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     7981 actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc
     8041 ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     8101 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     8161 aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca
     8221 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa
     8281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgct
     8341 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     8401 taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     8461 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     8521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     8641 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     8701 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     8761 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc
     8821 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     8881 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     8941 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     9001 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     9061 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     9121 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     9181 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     9241 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     9301 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     9361 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     9421 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     9481 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     9541 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     9601 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     9661 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     9721 actcctgcat taggaaat
//
