LOCUS       pMLS538                12657 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
COMMENT     Insert from cas9 cloning site 1-2 13 to 999
            Backbone from pDONR 221 2897 to 651
FEATURES             Location/Qualifiers
     source          1..12657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     join(570..615,617..651)
                     /locus_tag="attP1"
                     /label=attP1
     CDS             671..673
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and
     misc_feature    674..712
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     CDS             713..1633
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(5)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(5)
     CDS             2937..3914
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(1)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(1)
     CDS             5813..6818
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(2)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(2)
     intron          5990..6040
                     /locus_tag="intron sequence"
                     /label=intron sequence
     CDS             8206..8927
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(3)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(3)
     misc_feature    8639..8644
                     /locus_tag="BbsI"
                     /label=BbsI
     CDS             10127..10654
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(4)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(4)
     misc_feature    10655..10687
                     /locus_tag="Flexible Linker"
                     /label=Flexible Linker
     misc_feature    10688..10690
                     /locus_tag="NaeI"
                     /label=NaeI
     misc_feature    10691..10693
                     /locus_tag="NaeI(1)"
                     /label=NaeI
                     /label=NaeI(1)
     misc_feature    10694..10777
                     /locus_tag="egl-13 NLS"
                     /label=egl-13 NLS
     misc_feature    10778..10780
                     /locus_tag="Stop"
                     /label=Stop
     misc_recomb     complement(10792..10880)
                     /locus_tag="attP2"
                     /label=attP2
     primer_bind     complement(10898..10919)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(10918..10938)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     CDS             11051..11857
                     /locus_tag="KanR"
                     /label=KanR
     rep_origin      11927..12613
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcg atgactgctc caaagaagaa gcgtaaggta ccggtagaaa aaatggacaa
      721 gaagtactcc atcggactcg acatcggaac caactccgtc ggatgggccg tcatcaccga
      781 cgagtacaag gtcccatcca agaagttcaa ggtcctcgga aacaccgacc gtcactccat
      841 caagaagaac ctcatcggag ccctcctctt cgactccgga gagaccgccg aggccacccg
      901 tctcaagcgt accgcccgtc gtcgttacac ccgtcgtaag aaccgtatct gctacctcca
      961 ggagatcttc tccaacgaga tggccaaggt cgacgactcc ttcttccacc gtctcgagga
     1021 gtccttcctc gtcgaggagg acaagaagca cgagcgtcac ccaatcttcg gaaacatcgt
     1081 cgacgaggtc gcctaccacg agaagtaccc aaccatctac cacctccgta agaagctcgt
     1141 cgactccacc gacaaggccg acctccgtct catctacctc gccctcgccc acatgatcaa
     1201 gttccgtgga cacttcctca tcgagggaga cctcaaccca gacaactccg acgtcgacaa
     1261 gctcttcatc cagctcgtcc agacctacaa ccagctcttc gaggagaacc caatcaacgc
     1321 ctccggagtc gacgccaagg ccatcctctc cgcccgtctc tccaagtccc gtcgtctcga
     1381 gaacctcatc gcccagctcc caggagagaa gaagaacgga ctcttcggaa acctcatcgc
     1441 cctctccctc ggactcaccc caaacttcaa gtccaacttc gacctcgccg aggacgccaa
     1501 gctccagctc tccaaggaca cctacgacga cgacctcgac aacctcctcg cccagatcgg
     1561 agaccagtac gccgacctct tcctcgccgc caagaacctc tccgacgcca tcctcctctc
     1621 cgacatcctc cgtgtaagga gttgaacggc tgaaaaatcg atattttgag cgaaaaaagc
     1681 cggaaaaatg gattttatcg gataaaattt gattttttga gctgagaaat gcctttatag
     1741 acgaatttcc ttgtcaaatt gttaaaaact gcaaaatttg ccaaaaaaaa agcatttttt
     1801 atattttttt ttacaaaaaa ttccacaaaa atggcaaaat ttgactaaaa atgccaattt
     1861 tgtcgttttc tccgtgccac agcggccgaa aatcgatttt taagcgattt tcgggtgaaa
     1921 aagtgtaaaa accgactgaa aatccagctg aaaacgacaa aaacggtaag ttttagctaa
     1981 ttttcacctt tttttcgata tttttttact aaaaaaaaac cgaaaaattg agtttttttc
     2041 aaatttctgc aaaaattctg caattttagc aatttccata ctattttgac gccgaaaaag
     2101 cttaaaaata tgatttttag aggtttttaa tggaaaattc atggattttt caaatttttc
     2161 cacgaaaatc attgaaaaat ttgaaaaaat tgatttccta ttcaaagttc tagctaaaaa
     2221 ctgcaatttt agcaatttcc atactatttt agtactgaaa aagcctaaaa atgtgatttt
     2281 cagaggattt caatggaaaa ttcatggatt tttccaattt ttcaatgaaa accattgaaa
     2341 aaccctggaa aattgagttt ttcttcaaag tttaggaaat ttatgagaat ttttgaataa
     2401 aaaattttta aatttgaaaa aaattgaaat tttaattcta aattttaaag aaagttgatt
     2461 ttttaataat tttttttttt gaataaaaaa aaatcaactt tctttaaaat ttagaattaa
     2521 aatttcaatt tttttattca aaaattctca taaatttcgt aaacttttaa taaaaactca
     2581 atttttccga tttttttcga tgattttcac taaaaaattg gaaaaattca tcaatttcct
     2641 attgaaaacc tctaaaaatt atattttttt tgcttttccg acgtcaaaat agtatggaaa
     2701 atgctaaaat tgcagaattt ttccagaaat ttgaagaaaa aactcaattt tttccgtttt
     2761 ttcaatgatt ttcatgaaaa aattggaaaa ttttgtaaat tttccattga aaacctctga
     2821 aaatttggaa attttcgaat ttaaatgttt aaaaaaaatt gaatattttg cgagtttttg
     2881 aatttttttt gagaattttt gaattttttt cacccaaaat tttttcaatt tttcaggtca
     2941 acaccgagat caccaaggcc ccactctccg cctccatgat caagcgttac gacgagcacc
     3001 accaggacct caccctcctc aaggccctcg tccgtcagca gctcccagag aagtacaagg
     3061 agatcttctt cgaccagtcc aagaacggat acgccggata catcgacgga ggagcctccc
     3121 aggaggagtt ctacaagttc atcaagccaa tcctcgagaa gatggacgga accgaggagc
     3181 tcctcgtcaa gctcaaccgt gaggacctcc tccgtaagca gcgtaccttc gacaacggat
     3241 ccatcccaca ccagatccac ctcggagagc tccacgccat cctccgtcgt caggaggact
     3301 tctacccatt cctcaaggac aaccgtgaga agatcgagaa gatcctcacc ttccgtatcc
     3361 catactacgt cggaccactc gcccgtggaa actcccgttt cgcctggatg acccgtaagt
     3421 ccgaggagac catcacccca tggaacttcg aggaggtcgt cgacaaggga gcctccgccc
     3481 agtccttcat cgagcgtatg accaacttcg acaagaacct cccaaacgag aaggtcctcc
     3541 caaagcactc cctcctctac gagtacttca ccgtctacaa cgagctcacc aaggtcaagt
     3601 acgtcaccga gggaatgcgt aagccagcct tcctctccgg agagcagaag aaggccatcg
     3661 tcgacctcct cttcaagacc aaccgtaagg tcaccgtcaa gcagctcaag gaggactact
     3721 tcaagaagat cgagtgcttc gactccgtcg agatctccgg agtcgaggac cgtttcaacg
     3781 cctccctcgg aacctaccac gacctcctca agatcatcaa ggacaaggac ttcctcgaca
     3841 acgaggagaa cgaggacatc ctcgaggaca tcgtcctcac cctcaccctc ttcgaggacc
     3901 gtgagatgat cgaggtgctg aagctcgaaa atttggagct tttacacgag atttttgaag
     3961 aaaaagcctg aaaatcgaga gattttgagc tgtggaattc gttgaaatta cataattcgc
     4021 gcccgtaatt ctttaaaaaa acgctgaaat ttgcagattt tgccagaaag ttgaagaaaa
     4081 atctactttt ccggcattct ctattatttt catggaaaat tagaaaaatc catgaataaa
     4141 cagtgaaaat tgctgatttt acctgaaatt ttgaaaaaaa aaaaaatcta aaatcaaggt
     4201 tttaagccta aaaactgcag atttcagcca aaattgtgaa aaaagctgat tttttgctat
     4261 tttgagcttt tccatggggt ggaaattgcc aaaaattacg gaacattcac acaaatttgc
     4321 tgaataatcc aattttccag tgttttctag agtttccgta gaaattttga aaaaacccag
     4381 taaattttca atcggaaaac ctctaaaaac atcgtttcag gcctataaat agtaaaaatt
     4441 accgatttta ttcgaaaatt taaggaaaag tcgtgaaaaa cgagatttta agcctcaaaa
     4501 ctgcagattc tatctaaaat taaaaaaaaa aaacataaat atccgaattt ttggaggaaa
     4561 agcctgaaaa tcgatatttt aagcctcaaa actgaagatt tcagccaaaa aaaaaaacag
     4621 atttttcgct attttatgtg ccaaaaaacc acattccgag cttttccaga gtggaaattg
     4681 ccaaaaatta cagaattttc ccagaaatta gcaaaaaaaa aacccatttt tcaggcgttt
     4741 tcaacgattt tcatagcaaa tttgaaaaat ccatgaattt tttaattata gaaaaccctc
     4801 tgaaaattgc agttttaagc tattccagca ttcaaatagt atggaaattg ctaaaaattg
     4861 aagaattttc ccaaaaattt gatgaaaact tatttttctg gcgttttcga tgattttcat
     4921 ggaatttcga gaaaattaat gaattttcaa ttaaaaaacg tctgcaaaat cacatttttt
     4981 aaagcttttt cagcaacaaa atagtatgga aattgctttt taaaaaagcg gaatttttgg
     5041 aaaaaaggtt aaattttcag tcagtttttg cactttttga ctgaaaattc actgaaatta
     5101 gggaaaaatt ccggaaaaat gattttttcg aattgtaggg caaattttgc aatttcaagc
     5161 aatttccata atattttaat gctggaaaag ctcaaattta cgttttaatg ggattttagt
     5221 gaaaaattca tggattttcc gaaaaatttg caggaaaatc atagtaaaga cctcaaaaaa
     5281 tcaataaaaa atttaaaaaa ttggacaatc acatttctgc gtaaaatctg cactttttca
     5341 gtaaaaaata ccgattttcc gtgaaaattc tcgattttta atcgaaaatc tcgaaatttt
     5401 tacaaatttc agcaccaaaa tcgttgaaaa ttctgaaaat ttgaattttt tttccttgtt
     5461 ttttgagtaa aatttgcact ttttcagtaa agaataccga ttttccgtga aaattctcgg
     5521 tttttaatcg aaaatctcga aatttttaca aatttcagct ccgaaatagc tgaaaattgg
     5581 acaaaaattt caatttttgc cctgtttttt gagtaaaatc tgcacttttt agttgaaaaa
     5641 aaacacggat tttgtaggaa aatttgggaa aaatctccaa attttcacaa ttttccagta
     5701 aaaacagcaa tttttaaaac ggtttttaat caaaaatctt tagaattttc caaatttcag
     5761 caccaaaaaa gctaaaaatc gctgaaattt tcgacaaatt ccaaatttcc aggagcgtct
     5821 caagacctac gcccacctct tcgacgacaa ggtcatgaag cagctcaagc gtcgtcgtta
     5881 caccggatgg ggacgtctct cccgtaagct catcaacgga atccgtgaca agcagtccgg
     5941 aaagaccatc ctcgacttcc tcaagtccga cggattcgcc aaccgtaacg taagtttaaa
     6001 catgatttta ctaactaact aatctgattt aaattttcag ttcatgcagc tcatccacga
     6061 cgactccctc accttcaagg aggacatcca gaaggcccag gtctccggac agggagactc
     6121 cctccacgag cacatcgcca acctcgccgg atccccagcc atcaagaagg gaatcctcca
     6181 gaccgtcaag gtcgtcgacg agctcgtcaa ggtcatggga cgtcacaagc cagagaacat
     6241 cgtcatcgag atggcccgtg agaaccagac cacccagaag ggacagaaga actcccgtga
     6301 gcgtatgaag cgtatcgagg agggaatcaa ggagctcgga tcccagatcc tcaaggagca
     6361 cccagtcgag aacacccagc tccagaacga gaagctctac ctctactacc tccagaacgg
     6421 acgtgacatg tacgtcgacc aggagctcga catcaaccgt ctctccgact acgacgtcga
     6481 ccacatcgtc ccacagtcct tcctcaagga cgactccatc gacaacaagg tcctcacccg
     6541 ttccgacaag aaccgtggaa agtccgacaa cgtcccatcc gaggaggtcg tcaagaagat
     6601 gaagaactac tggcgtcagc tcctcaacgc caagctcatc acccagcgta agttcgacaa
     6661 cctcaccaag gccgagcgtg gaggactctc cgagctcgac aaggccggat tcatcaagcg
     6721 tcagctcgtc gagacccgtc agatcaccaa gcacgtcgcc cagatcctcg actcccgtat
     6781 gaacaccaag tacgacgaga acgacaagct catccgtggt tggtttttcc gagagaaaaa
     6841 cgctgaaaaa tgccgaaaat tttgaatttg caggcttttt aggtgcaaaa gtacggaaaa
     6901 atcggaaaaa aatcaggaaa acatggggaa aatccaattt tccagaaaaa atgtttgaaa
     6961 aaattgaaaa cttttgtttt tttagtaata aagttttaaa aaatggcaaa aatagctgaa
     7021 aacagcgaaa aaaaaaaatg tttttttcag gaaaaaattt gcaaaaaaat gaagagcaac
     7081 aataattccc ttttttttct gtgttttttt cggaaaaatt gagatcgaaa acgaaaaaca
     7141 gcgaaaattc ccaattttcc cagtttttaa aacatttttt tgtaaaaatt tggaaaaaac
     7201 gagttttttc agaaaaaaat gtgaaaaaaa aggtaaatta ccaatttatg cttttttttc
     7261 gatcgagtaa aaaatcgatt tttccaagat ttttcgcgaa aaattcaaaa aaaaagaacg
     7321 aaaattctcg attttctact ttttttcgaa aaaaaaagga aattatagat ttttccaaga
     7381 tttttcgcga aaaaattcga aaaaaatgtt tttttcgaaa atttcagatt ttctaatttt
     7441 ttcagaagaa ttcgaaaaaa aaaatcaaaa aattcgcaat ttttagattt ttttaaaatc
     7501 tcaattttct ataattattt tcctaaaaaa atgtaattaa aaaaccgcta aaaacggtaa
     7561 attccaaatt tttgcatttt ttcaaaaaaa atttgaaaaa aaaaatcaga aaattctcaa
     7621 atctattccc tttttttaaa atttttccca aaccaaaaaa aaaaacggta aattttcaaa
     7681 tttagcattt ttccaaaatt aaacataaaa aaaaacgcaa atttccaaat ttcgtttttt
     7741 tttcaaaaaa gttgaaaaat ccagaaaata ccctcttgtc catgaaaaaa tacgaaaatt
     7801 ttcaataaaa aatcaatttc ttcttaaaaa attaattctc gatttttcca tgaaaaaaaa
     7861 tcaaatatta attaaatttg aaaaaaaaaa gaatttatct aatagtttcg ttttttttca
     7921 gaggaaaaat ttgaaaaaaa aaacagtaaa ttctctttct ttttgaaaaa aaaaattatc
     7981 gattcttcca agttttttcc cgaaaaaatc gacaaaaaaa cgagaatttc cgattttcta
     8041 tttttttttt caaaaaaatg tgaaaaaaaa aattaaaaaa ttccaatttt gcattttttt
     8101 ttcaaaaaaa atttaaaaac accgtataat tcccgatttt tccatgaaaa aatacgaaaa
     8161 attccaataa aaatcaacta ttttaaaaat ttgccgtttt ttcagaggtc aaggtcatca
     8221 ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccagttctac aaggtccgtg
     8281 agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc ggaaccgccc
     8341 tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac aaggtctacg
     8401 acgtccgtaa gatgatcgcc aagtccgagc aggagatcgg aaaggccacc gccaagtact
     8461 tcttctactc caacatcatg aacttcttca agaccgagat caccctcgcc aacggagaga
     8521 tccgtaagcg tccactcatc gagaccaacg gagagaccgg agagatcgtc tgggacaagg
     8581 gacgtgactt cgccaccgtc cgtaaggtcc tctccatgcc acaggtcaac atcgtcaaga
     8641 agaccgaggt ccagaccgga ggattctcca aggagtccat cctcccaaag cgtaactccg
     8701 acaagctcat cgcccgtaag aaggactggg acccaaagaa gtacggagga ttcgactccc
     8761 caaccgtcgc ctactccgtc ctcgtcgtcg ccaaggtcga gaagggaaag tccaagaagc
     8821 tcaagtccgt caaggagctc ctcggaatca ccatcatgga gcgttcctcc ttcgagaaga
     8881 acccaatcga cttcctcgag gccaagggat acaaggaggt caagaaggtg cttacaattt
     8941 tctggagaaa aatattttaa aaaataacga aaatggaccg aatttttgga gaaaatttta
     9001 aaaaaaacga catttttccc aaaaaattga aaaatttccg aaaaaacgtt caaaattttc
     9061 aaaaaaggag catttttcga aaaaaaaaac gataatacgg aaattttttg attttccagt
     9121 ttttttctca aagaaaatcg gaaaaaggtt ttttttcttt aaaaacgtcc aaaatttttc
     9181 gaaaaaagag aaaattttcg attttccgat ttttttcaga aaaaaattga aaaaaaaaga
     9241 aattttccga ttttctcaga gaaagacgaa aagacgatat ttttcggaaa aaagttgaaa
     9301 aatcaaaaaa aaaaccgaaa aacggccgaa attttctaaa aattggaaat attgtcgatt
     9361 tttcgaaaaa aaagagtcaa aagttttcaa aaaaatatat tttttcgatt ttccgttttt
     9421 ttttttcagt aaacattgaa acaaacgaaa tttttccaaa aaaatccaat tattttccca
     9481 aatatcgaaa tatttttgat tttccgtttt tttcagaaaa aagttaaaaa aaaaagggaa
     9541 aattaagatt ttcctgattt tttttgagaa aaaatcgata aaaattgaat attctggaaa
     9601 aaacctttaa aaaattcgaa ttaaatcgaa aaacgtccaa aattttctaa aaaattgaaa
     9661 ttttttttga aaaaaaacgg aaaaatttag atttcccgac ttttttcaat gaaaaatcga
     9721 attttcgttg acttttcatt aaaaaatcca aacgcatttt tttccactaa aatctcgaaa
     9781 aagtgtcata ttgcaccata ttgtttccac attcatatta ttttgagcta aatcaggtga
     9841 ttttctgtat tttgatacta atttctattt ttagaaaaaa tgtgggaaca atatggtgca
     9901 atatgacatt ttttcgagat tttagtggag aaaaaatgcg tttggaaacg aattttataa
     9961 taaaaagtca acgaaaattc gatttttcat ggaaaaatag ggaaatcgaa aacaaatttg
    10021 cttttttttc aaaaaaaaaa acggaaattc gaaatttttc cgttctttca aaaaaaaaat
    10081 cgattttttt ggaaattttt tttttcaaat ttttaaaaat ttccaggacc tcatcatcaa
    10141 gctcccaaag tactccctct tcgagctcga gaacggacgt aagcgtatgc tcgcctccgc
    10201 cggagagctc cagaagggaa acgagctcgc cctcccatcc aagtacgtca acttcctcta
    10261 cctcgcctcc cactacgaga agctcaaggg atccccagag gacaacgagc agaagcagct
    10321 cttcgtcgag cagcacaagc actacctcga cgagatcatc gagcagatct ccgagttctc
    10381 caagcgtgtc atcctcgccg acgccaacct cgacaaggtc ctctccgcct acaacaagca
    10441 ccgtgacaag ccaatccgtg agcaggccga gaacatcatc cacctcttca ccctcaccaa
    10501 cctcggagcc ccagccgcct tcaagtactt cgacaccacc atcgaccgta agcgttacac
    10561 ctccaccaag gaggtcctcg acgccaccct catccaccag tccatcaccg gactctacga
    10621 gacccgtatc gacctctccc agctcggagg agactctgga ggatctggag gaactggagg
    10681 atcttccgcc ggcgggagct ctatgagccg tagacgaaaa gcgaatccga caaaactgag
    10741 tgaaaacgcg aagaagcttg ccaaggaagt tgaaaattaa cacccagctt tcttgtacaa
    10801 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
    10861 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
    10921 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
    10981 acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
    11041 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
    11101 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
    11161 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
    11221 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
    11281 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
    11341 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
    11401 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
    11461 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
    11521 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
    11581 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
    11641 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
    11701 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
    11761 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
    11821 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
    11881 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
    11941 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    12001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    12061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    12121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    12181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    12241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    12301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    12361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    12421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    12481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    12541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    12601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
