LOCUS       pMLS544                18467 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
COMMENT     Promoter from pCFJ183 1 to 525
            Gene from pMLS538 652 to 10791
            UTR from pCM1_36 tbb-UTR 734 to 1086
            Backbone from pCFJ150 7607 to 5923
FEATURES             Location/Qualifiers
     source          1..18467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..145
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             782..1441
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1539..2221
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_recomb     2355..3339
                     /locus_tag="ttTi5605 left recomb"
                     /label=ttTi5605 left recomb
     misc_feature    3222..3241
                     /locus_tag="oCF176"
                     /label=oCF176
     gene            3691..5808
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label=C.Briggsae unc-119(+)
     misc_feature    complement(3806..3825)
                     /locus_tag="oCF419"
                     /label=oCF419
     misc_feature    complement(3910..3929)
                     /locus_tag="oCF132 and oCF169"
                     /label=oCF132 and oCF169
     misc_feature    complement(4114..4133)
                     /locus_tag="oCF126"
                     /label=oCF126
     CDS             4679..5524
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
     primer_bind     5881..5901
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_recomb     5917..5923
                     /locus_tag="attR4 recomb"
                     /label=attR4 recomb
     promoter        5938..6441
                     /locus_tag="Pmex-5"
                     /label=Pmex-5
     primer_bind     5938..5957
                     /locus_tag="Forward primer"
                     /label=Forward primer
     primer_bind     complement(6421..6441)
                     /locus_tag="reverse primer"
                     /label=reverse primer
     CDS             6468..6470
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and
     misc_feature    6471..6509
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     CDS             6510..7430
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(5)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(5)
     CDS             8734..9711
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(1)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(1)
     CDS             11610..12615
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(2)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(2)
     intron          11787..11837
                     /locus_tag="intron sequence"
                     /label=intron sequence
     CDS             14003..14724
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(3)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(3)
     misc_feature    14436..14441
                     /locus_tag="BbsI"
                     /label=BbsI
     CDS             15924..16451
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(4)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(4)
     misc_feature    16452..16484
                     /locus_tag="Flexible Linker"
                     /label=Flexible Linker
     misc_feature    16485..16487
                     /locus_tag="NaeI"
                     /label=NaeI
     misc_feature    16488..16490
                     /locus_tag="NaeI(1)"
                     /label=NaeI
                     /label=NaeI(1)
     misc_feature    16491..16574
                     /locus_tag="egl-13 NLS"
                     /label=egl-13 NLS
     misc_feature    16575..16577
                     /locus_tag="Stop"
                     /label=Stop
     misc_recomb     complement(16589..16602)
                     /locus_tag="AttR2"
                     /label=AttR2
     3'UTR           16603..16934
                     /locus_tag="tbb-2 utr"
                     /label=tbb-2 utr
     misc_feature    16605..16607
                     /locus_tag="Stop(1)"
                     /label=Stop
                     /label=Stop(1)
     misc_recomb     complement(16935..16941)
                     /locus_tag="attL3"
                     /label=attL3
     misc_recomb     complement(16942..16955)
                     /locus_tag="attR3"
                     /label=attR3
     primer_bind     complement(16963..16980)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     17040..18467
                     /locus_tag="ttTi5605 right recomb"
                     /label=ttTi5605 right recomb
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgggt
     5941 tttcacaacg gcaaaatatc agtttttaaa aaattaaacc ataaaacaaa taatataacc
     6001 caatttttac atcaaaccac aagaaaaaaa tacatttggg cccacggata aagaaattaa
     6061 aaaaatacat tttttaaagg cgcaccgaat taaaattcat ttgggtctta ccgcgtatac
     6121 cgtactccgt ttgtttgatc atttttgtca gcgctggcgg ttgttttttc atttcatttc
     6181 tgcttcaaag acgttttctc gaataatttt tcgtttattc tcttttttaa aattaatttc
     6241 tagccgtaaa tgttataaat tcacccattt aacgcaaatt tcatggtaat ctcatggaaa
     6301 aatgcagttt ctttgttaaa gaaagcttaa atagcaaaaa ttccccgact ttccccaaaa
     6361 tcctgctcga ttttccgttt tctcattgta ttctctctta attaatttta tcgataatca
     6421 attgaatgtt tcagacagag acaagtttgt acaaaaaagc aggctcgatg actgctccaa
     6481 agaagaagcg taaggtaccg gtagaaaaaa tggacaagaa gtactccatc ggactcgaca
     6541 tcggaaccaa ctccgtcgga tgggccgtca tcaccgacga gtacaaggtc ccatccaaga
     6601 agttcaaggt cctcggaaac accgaccgtc actccatcaa gaagaacctc atcggagccc
     6661 tcctcttcga ctccggagag accgccgagg ccacccgtct caagcgtacc gcccgtcgtc
     6721 gttacacccg tcgtaagaac cgtatctgct acctccagga gatcttctcc aacgagatgg
     6781 ccaaggtcga cgactccttc ttccaccgtc tcgaggagtc cttcctcgtc gaggaggaca
     6841 agaagcacga gcgtcaccca atcttcggaa acatcgtcga cgaggtcgcc taccacgaga
     6901 agtacccaac catctaccac ctccgtaaga agctcgtcga ctccaccgac aaggccgacc
     6961 tccgtctcat ctacctcgcc ctcgcccaca tgatcaagtt ccgtggacac ttcctcatcg
     7021 agggagacct caacccagac aactccgacg tcgacaagct cttcatccag ctcgtccaga
     7081 cctacaacca gctcttcgag gagaacccaa tcaacgcctc cggagtcgac gccaaggcca
     7141 tcctctccgc ccgtctctcc aagtcccgtc gtctcgagaa cctcatcgcc cagctcccag
     7201 gagagaagaa gaacggactc ttcggaaacc tcatcgccct ctccctcgga ctcaccccaa
     7261 acttcaagtc caacttcgac ctcgccgagg acgccaagct ccagctctcc aaggacacct
     7321 acgacgacga cctcgacaac ctcctcgccc agatcggaga ccagtacgcc gacctcttcc
     7381 tcgccgccaa gaacctctcc gacgccatcc tcctctccga catcctccgt gtaaggagtt
     7441 gaacggctga aaaatcgata ttttgagcga aaaaagccgg aaaaatggat tttatcggat
     7501 aaaatttgat tttttgagct gagaaatgcc tttatagacg aatttccttg tcaaattgtt
     7561 aaaaactgca aaatttgcca aaaaaaaagc attttttata ttttttttta caaaaaattc
     7621 cacaaaaatg gcaaaatttg actaaaaatg ccaattttgt cgttttctcc gtgccacagc
     7681 ggccgaaaat cgatttttaa gcgattttcg ggtgaaaaag tgtaaaaacc gactgaaaat
     7741 ccagctgaaa acgacaaaaa cggtaagttt tagctaattt tcaccttttt ttcgatattt
     7801 ttttactaaa aaaaaaccga aaaattgagt ttttttcaaa tttctgcaaa aattctgcaa
     7861 ttttagcaat ttccatacta ttttgacgcc gaaaaagctt aaaaatatga tttttagagg
     7921 tttttaatgg aaaattcatg gatttttcaa atttttccac gaaaatcatt gaaaaatttg
     7981 aaaaaattga tttcctattc aaagttctag ctaaaaactg caattttagc aatttccata
     8041 ctattttagt actgaaaaag cctaaaaatg tgattttcag aggatttcaa tggaaaattc
     8101 atggattttt ccaatttttc aatgaaaacc attgaaaaac cctggaaaat tgagtttttc
     8161 ttcaaagttt aggaaattta tgagaatttt tgaataaaaa atttttaaat ttgaaaaaaa
     8221 ttgaaatttt aattctaaat tttaaagaaa gttgattttt taataatttt tttttttgaa
     8281 taaaaaaaaa tcaactttct ttaaaattta gaattaaaat ttcaattttt ttattcaaaa
     8341 attctcataa atttcgtaaa cttttaataa aaactcaatt tttccgattt ttttcgatga
     8401 ttttcactaa aaaattggaa aaattcatca atttcctatt gaaaacctct aaaaattata
     8461 ttttttttgc ttttccgacg tcaaaatagt atggaaaatg ctaaaattgc agaatttttc
     8521 cagaaatttg aagaaaaaac tcaatttttt ccgttttttc aatgattttc atgaaaaaat
     8581 tggaaaattt tgtaaatttt ccattgaaaa cctctgaaaa tttggaaatt ttcgaattta
     8641 aatgtttaaa aaaaattgaa tattttgcga gtttttgaat tttttttgag aatttttgaa
     8701 tttttttcac ccaaaatttt ttcaattttt caggtcaaca ccgagatcac caaggcccca
     8761 ctctccgcct ccatgatcaa gcgttacgac gagcaccacc aggacctcac cctcctcaag
     8821 gccctcgtcc gtcagcagct cccagagaag tacaaggaga tcttcttcga ccagtccaag
     8881 aacggatacg ccggatacat cgacggagga gcctcccagg aggagttcta caagttcatc
     8941 aagccaatcc tcgagaagat ggacggaacc gaggagctcc tcgtcaagct caaccgtgag
     9001 gacctcctcc gtaagcagcg taccttcgac aacggatcca tcccacacca gatccacctc
     9061 ggagagctcc acgccatcct ccgtcgtcag gaggacttct acccattcct caaggacaac
     9121 cgtgagaaga tcgagaagat cctcaccttc cgtatcccat actacgtcgg accactcgcc
     9181 cgtggaaact cccgtttcgc ctggatgacc cgtaagtccg aggagaccat caccccatgg
     9241 aacttcgagg aggtcgtcga caagggagcc tccgcccagt ccttcatcga gcgtatgacc
     9301 aacttcgaca agaacctccc aaacgagaag gtcctcccaa agcactccct cctctacgag
     9361 tacttcaccg tctacaacga gctcaccaag gtcaagtacg tcaccgaggg aatgcgtaag
     9421 ccagccttcc tctccggaga gcagaagaag gccatcgtcg acctcctctt caagaccaac
     9481 cgtaaggtca ccgtcaagca gctcaaggag gactacttca agaagatcga gtgcttcgac
     9541 tccgtcgaga tctccggagt cgaggaccgt ttcaacgcct ccctcggaac ctaccacgac
     9601 ctcctcaaga tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctc
     9661 gaggacatcg tcctcaccct caccctcttc gaggaccgtg agatgatcga ggtgctgaag
     9721 ctcgaaaatt tggagctttt acacgagatt tttgaagaaa aagcctgaaa atcgagagat
     9781 tttgagctgt ggaattcgtt gaaattacat aattcgcgcc cgtaattctt taaaaaaacg
     9841 ctgaaatttg cagattttgc cagaaagttg aagaaaaatc tacttttccg gcattctcta
     9901 ttattttcat ggaaaattag aaaaatccat gaataaacag tgaaaattgc tgattttacc
     9961 tgaaattttg aaaaaaaaaa aaatctaaaa tcaaggtttt aagcctaaaa actgcagatt
    10021 tcagccaaaa ttgtgaaaaa agctgatttt ttgctatttt gagcttttcc atggggtgga
    10081 aattgccaaa aattacggaa cattcacaca aatttgctga ataatccaat tttccagtgt
    10141 tttctagagt ttccgtagaa attttgaaaa aacccagtaa attttcaatc ggaaaacctc
    10201 taaaaacatc gtttcaggcc tataaatagt aaaaattacc gattttattc gaaaatttaa
    10261 ggaaaagtcg tgaaaaacga gattttaagc ctcaaaactg cagattctat ctaaaattaa
    10321 aaaaaaaaaa cataaatatc cgaatttttg gaggaaaagc ctgaaaatcg atattttaag
    10381 cctcaaaact gaagatttca gccaaaaaaa aaaacagatt tttcgctatt ttatgtgcca
    10441 aaaaaccaca ttccgagctt ttccagagtg gaaattgcca aaaattacag aattttccca
    10501 gaaattagca aaaaaaaaac ccatttttca ggcgttttca acgattttca tagcaaattt
    10561 gaaaaatcca tgaatttttt aattatagaa aaccctctga aaattgcagt tttaagctat
    10621 tccagcattc aaatagtatg gaaattgcta aaaattgaag aattttccca aaaatttgat
    10681 gaaaacttat ttttctggcg ttttcgatga ttttcatgga atttcgagaa aattaatgaa
    10741 ttttcaatta aaaaacgtct gcaaaatcac attttttaaa gctttttcag caacaaaata
    10801 gtatggaaat tgctttttaa aaaagcggaa tttttggaaa aaaggttaaa ttttcagtca
    10861 gtttttgcac tttttgactg aaaattcact gaaattaggg aaaaattccg gaaaaatgat
    10921 tttttcgaat tgtagggcaa attttgcaat ttcaagcaat ttccataata ttttaatgct
    10981 ggaaaagctc aaatttacgt tttaatggga ttttagtgaa aaattcatgg attttccgaa
    11041 aaatttgcag gaaaatcata gtaaagacct caaaaaatca ataaaaaatt taaaaaattg
    11101 gacaatcaca tttctgcgta aaatctgcac tttttcagta aaaaataccg attttccgtg
    11161 aaaattctcg atttttaatc gaaaatctcg aaatttttac aaatttcagc accaaaatcg
    11221 ttgaaaattc tgaaaatttg aatttttttt ccttgttttt tgagtaaaat ttgcactttt
    11281 tcagtaaaga ataccgattt tccgtgaaaa ttctcggttt ttaatcgaaa atctcgaaat
    11341 ttttacaaat ttcagctccg aaatagctga aaattggaca aaaatttcaa tttttgccct
    11401 gttttttgag taaaatctgc actttttagt tgaaaaaaaa cacggatttt gtaggaaaat
    11461 ttgggaaaaa tctccaaatt ttcacaattt tccagtaaaa acagcaattt ttaaaacggt
    11521 ttttaatcaa aaatctttag aattttccaa atttcagcac caaaaaagct aaaaatcgct
    11581 gaaattttcg acaaattcca aatttccagg agcgtctcaa gacctacgcc cacctcttcg
    11641 acgacaaggt catgaagcag ctcaagcgtc gtcgttacac cggatgggga cgtctctccc
    11701 gtaagctcat caacggaatc cgtgacaagc agtccggaaa gaccatcctc gacttcctca
    11761 agtccgacgg attcgccaac cgtaacgtaa gtttaaacat gattttacta actaactaat
    11821 ctgatttaaa ttttcagttc atgcagctca tccacgacga ctccctcacc ttcaaggagg
    11881 acatccagaa ggcccaggtc tccggacagg gagactccct ccacgagcac atcgccaacc
    11941 tcgccggatc cccagccatc aagaagggaa tcctccagac cgtcaaggtc gtcgacgagc
    12001 tcgtcaaggt catgggacgt cacaagccag agaacatcgt catcgagatg gcccgtgaga
    12061 accagaccac ccagaaggga cagaagaact cccgtgagcg tatgaagcgt atcgaggagg
    12121 gaatcaagga gctcggatcc cagatcctca aggagcaccc agtcgagaac acccagctcc
    12181 agaacgagaa gctctacctc tactacctcc agaacggacg tgacatgtac gtcgaccagg
    12241 agctcgacat caaccgtctc tccgactacg acgtcgacca catcgtccca cagtccttcc
    12301 tcaaggacga ctccatcgac aacaaggtcc tcacccgttc cgacaagaac cgtggaaagt
    12361 ccgacaacgt cccatccgag gaggtcgtca agaagatgaa gaactactgg cgtcagctcc
    12421 tcaacgccaa gctcatcacc cagcgtaagt tcgacaacct caccaaggcc gagcgtggag
    12481 gactctccga gctcgacaag gccggattca tcaagcgtca gctcgtcgag acccgtcaga
    12541 tcaccaagca cgtcgcccag atcctcgact cccgtatgaa caccaagtac gacgagaacg
    12601 acaagctcat ccgtggttgg tttttccgag agaaaaacgc tgaaaaatgc cgaaaatttt
    12661 gaatttgcag gctttttagg tgcaaaagta cggaaaaatc ggaaaaaaat caggaaaaca
    12721 tggggaaaat ccaattttcc agaaaaaatg tttgaaaaaa ttgaaaactt ttgttttttt
    12781 agtaataaag ttttaaaaaa tggcaaaaat agctgaaaac agcgaaaaaa aaaaatgttt
    12841 ttttcaggaa aaaatttgca aaaaaatgaa gagcaacaat aattcccttt tttttctgtg
    12901 tttttttcgg aaaaattgag atcgaaaacg aaaaacagcg aaaattccca attttcccag
    12961 tttttaaaac atttttttgt aaaaatttgg aaaaaacgag ttttttcaga aaaaaatgtg
    13021 aaaaaaaagg taaattacca atttatgctt ttttttcgat cgagtaaaaa atcgattttt
    13081 ccaagatttt tcgcgaaaaa ttcaaaaaaa aagaacgaaa attctcgatt ttctactttt
    13141 tttcgaaaaa aaaaggaaat tatagatttt tccaagattt ttcgcgaaaa aattcgaaaa
    13201 aaatgttttt ttcgaaaatt tcagattttc taattttttc agaagaattc gaaaaaaaaa
    13261 atcaaaaaat tcgcaatttt tagatttttt taaaatctca attttctata attattttcc
    13321 taaaaaaatg taattaaaaa accgctaaaa acggtaaatt ccaaattttt gcattttttc
    13381 aaaaaaaatt tgaaaaaaaa aatcagaaaa ttctcaaatc tattcccttt ttttaaaatt
    13441 tttcccaaac caaaaaaaaa aacggtaaat tttcaaattt agcatttttc caaaattaaa
    13501 cataaaaaaa aacgcaaatt tccaaatttc gttttttttt caaaaaagtt gaaaaatcca
    13561 gaaaataccc tcttgtccat gaaaaaatac gaaaattttc aataaaaaat caatttcttc
    13621 ttaaaaaatt aattctcgat ttttccatga aaaaaaatca aatattaatt aaatttgaaa
    13681 aaaaaaagaa tttatctaat agtttcgttt tttttcagag gaaaaatttg aaaaaaaaaa
    13741 cagtaaattc tctttctttt tgaaaaaaaa aattatcgat tcttccaagt tttttcccga
    13801 aaaaatcgac aaaaaaacga gaatttccga ttttctattt tttttttcaa aaaaatgtga
    13861 aaaaaaaaat taaaaaattc caattttgca tttttttttc aaaaaaaatt taaaaacacc
    13921 gtataattcc cgatttttcc atgaaaaaat acgaaaaatt ccaataaaaa tcaactattt
    13981 taaaaatttg ccgttttttc agaggtcaag gtcatcaccc tcaagtccaa gctcgtctcc
    14041 gacttccgta aggacttcca gttctacaag gtccgtgaga tcaacaacta ccaccacgcc
    14101 cacgacgcct acctcaacgc cgtcgtcgga accgccctca tcaagaagta cccaaagctc
    14161 gagtccgagt tcgtctacgg agactacaag gtctacgacg tccgtaagat gatcgccaag
    14221 tccgagcagg agatcggaaa ggccaccgcc aagtacttct tctactccaa catcatgaac
    14281 ttcttcaaga ccgagatcac cctcgccaac ggagagatcc gtaagcgtcc actcatcgag
    14341 accaacggag agaccggaga gatcgtctgg gacaagggac gtgacttcgc caccgtccgt
    14401 aaggtcctct ccatgccaca ggtcaacatc gtcaagaaga ccgaggtcca gaccggagga
    14461 ttctccaagg agtccatcct cccaaagcgt aactccgaca agctcatcgc ccgtaagaag
    14521 gactgggacc caaagaagta cggaggattc gactccccaa ccgtcgccta ctccgtcctc
    14581 gtcgtcgcca aggtcgagaa gggaaagtcc aagaagctca agtccgtcaa ggagctcctc
    14641 ggaatcacca tcatggagcg ttcctccttc gagaagaacc caatcgactt cctcgaggcc
    14701 aagggataca aggaggtcaa gaaggtgctt acaattttct ggagaaaaat attttaaaaa
    14761 ataacgaaaa tggaccgaat ttttggagaa aattttaaaa aaaacgacat ttttcccaaa
    14821 aaattgaaaa atttccgaaa aaacgttcaa aattttcaaa aaaggagcat ttttcgaaaa
    14881 aaaaaacgat aatacggaaa ttttttgatt ttccagtttt tttctcaaag aaaatcggaa
    14941 aaaggttttt tttctttaaa aacgtccaaa atttttcgaa aaaagagaaa attttcgatt
    15001 ttccgatttt tttcagaaaa aaattgaaaa aaaaagaaat tttccgattt tctcagagaa
    15061 agacgaaaag acgatatttt tcggaaaaaa gttgaaaaat caaaaaaaaa accgaaaaac
    15121 ggccgaaatt ttctaaaaat tggaaatatt gtcgattttt cgaaaaaaaa gagtcaaaag
    15181 ttttcaaaaa aatatatttt ttcgattttc cgtttttttt tttcagtaaa cattgaaaca
    15241 aacgaaattt ttccaaaaaa atccaattat tttcccaaat atcgaaatat ttttgatttt
    15301 ccgttttttt cagaaaaaag ttaaaaaaaa aagggaaaat taagattttc ctgatttttt
    15361 ttgagaaaaa atcgataaaa attgaatatt ctggaaaaaa cctttaaaaa attcgaatta
    15421 aatcgaaaaa cgtccaaaat tttctaaaaa attgaaattt tttttgaaaa aaaacggaaa
    15481 aatttagatt tcccgacttt tttcaatgaa aaatcgaatt ttcgttgact tttcattaaa
    15541 aaatccaaac gcattttttt ccactaaaat ctcgaaaaag tgtcatattg caccatattg
    15601 tttccacatt catattattt tgagctaaat caggtgattt tctgtatttt gatactaatt
    15661 tctattttta gaaaaaatgt gggaacaata tggtgcaata tgacattttt tcgagatttt
    15721 agtggagaaa aaatgcgttt ggaaacgaat tttataataa aaagtcaacg aaaattcgat
    15781 ttttcatgga aaaataggga aatcgaaaac aaatttgctt ttttttcaaa aaaaaaaacg
    15841 gaaattcgaa atttttccgt tctttcaaaa aaaaaatcga tttttttgga aatttttttt
    15901 ttcaaatttt taaaaatttc caggacctca tcatcaagct cccaaagtac tccctcttcg
    15961 agctcgagaa cggacgtaag cgtatgctcg cctccgccgg agagctccag aagggaaacg
    16021 agctcgccct cccatccaag tacgtcaact tcctctacct cgcctcccac tacgagaagc
    16081 tcaagggatc cccagaggac aacgagcaga agcagctctt cgtcgagcag cacaagcact
    16141 acctcgacga gatcatcgag cagatctccg agttctccaa gcgtgtcatc ctcgccgacg
    16201 ccaacctcga caaggtcctc tccgcctaca acaagcaccg tgacaagcca atccgtgagc
    16261 aggccgagaa catcatccac ctcttcaccc tcaccaacct cggagcccca gccgccttca
    16321 agtacttcga caccaccatc gaccgtaagc gttacacctc caccaaggag gtcctcgacg
    16381 ccaccctcat ccaccagtcc atcaccggac tctacgagac ccgtatcgac ctctcccagc
    16441 tcggaggaga ctctggagga tctggaggaa ctggaggatc ttccgccggc gggagctcta
    16501 tgagccgtag acgaaaagcg aatccgacaa aactgagtga aaacgcgaag aagcttgcca
    16561 aggaagttga aaattaacac ccagctttct tgtacaaagt gggataaatg caaaatcctt
    16621 tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta
    16681 atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc
    16741 atctgagtga agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacaatctct
    16801 cttcaattcc attttgaagt gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa
    16861 atatttgtat tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca
    16921 agaaaaaagt ctcacaactt tattatacat agttgataat tcactggccg tcgttttaca
    16981 ctcgagacgt acggtgcgcg cgatgcattc gaagatctgc ccactagtga gtcgtattat
    17041 aagtgcaagt aagatcagtg tttgtttcga tatcagtctg tttcgtaacg gtcttctgta
    17101 taactacaaa aaagaataaa aaaccgtatc acaactgaga aaaaagacaa aacttattga
    17161 acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag tttttctcca aaataacgca
    17221 cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg tggaactcct tatcaagatt
    17281 accattattg tctgtcagaa ttgatatttc aattatttca tatctagaca aacccgggtg
    17341 tcttgcattt attagtactg tgagtttaca tacaaacgag gcttactcag ataacataga
    17401 gtcacctcta attagcaaaa acccatttct aattagctat ttcgaccagt tctaaagtca
    17461 ccgtaattag aaatggtttt tgtagctttt ctaattagaa atgaccccga gataagtgaa
    17521 atatggcatt ttctgggttt tctagtaaga aatgatcccg cacgtagtga ttccgcgtta
    17581 caagccaaat tcaaattaaa aaagtatatt acacaattca agttacatta ttttcaggaa
    17641 acaatggcga agagcaaagc agagattcaa aaggaggaaa gagacaacag atatgaacga
    17701 agacttcttg aaaagagaaa gagacatgag gtgagtaacc atgataataa ataatcaagt
    17761 aagggctata gatttataaa ttctggagtt aacagtttcg aaaaaaagta gttttcatat
    17821 gtgcgaagga atatcaaaaa taggggaata gcctaaacga gtgagaaaat taaatgtttg
    17881 ttattttttc aaaaatgtct gcagggccgt tatcctttca aatgcactct atcaaacata
    17941 gatcatatcc atcatatctt atccagaatt tatatcatat ccaagattga gagcgtttca
    18001 aggaaaatct caaacaacca tacaattttg tagatcgaca acaaggcctc cacttcttca
    18061 tcgatggcct ctgcagcacc tgcaccacca agtttgtatt tttcatatta atataaagtg
    18121 agccacgcat agtcgttttt aggatttttg ccagatcgat gtcgcgcaaa cagactgttt
    18181 ttttttcttt gaaagccgtg cgtgacatgg gccacggatt gtaacgccgc gctggatgga
    18241 taaaaattta ttcagttttt tagcctttac aaaatatggt tcgactactt tctttatttt
    18301 tacattttta taggtgctca ttgagctgag atattttggg tataatcgga aaatcgctga
    18361 ttttggtcat ttttctgcac taaagattac acaaaaagtt cagtatttag attcattgtt
    18421 gaaaattggg gatgatctac agatacatat cgccgtgttc aaaaaca
//
