LOCUS       pMLS714                16289 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
COMMENT     Promoter from pCFJ183 1 to 525
            Gene from pMLS538 652 to 10791
            UTR from pCM1.36 734 to 1086
            Backbone from pMLS713 4279 to 2596
FEATURES             Location/Qualifiers
     source          1..16289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    72..99
                     /locus_tag="Artificial 3'ITR"
                     /label=Artificial 3'ITR
     misc_feature    100..321
                     /locus_tag="Mos1 drosophila"
                     /label=Mos1 drosophila
     misc_feature    complement(300..322)
                     /locus_tag="oCF1210"
                     /label=oCF1210
     misc_feature    323..345
                     /locus_tag="syntron3(1)"
                     /label=syntron3
                     /label=syntron3(1)
     misc_feature    323..345
                     /locus_tag="GFP w/ syntrons(1)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(1)
     misc_feature    complement(323..325)
                     /locus_tag="Sap Trap gg Overlap(1)"
                     /label=Sap Trap gg Overlap
                     /label=Sap Trap gg Overlap(1)
     misc_feature    346..379
                     /locus_tag="Lox2272(1)"
                     /label=Lox2272
                     /label=Lox2272(1)
     gene            complement(380..2497)
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label=C.Briggsae unc-119(+)
     CDS             complement(664..1509)
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
     misc_feature    complement(771..821)
                     /locus_tag="Intron(2)"
                     /label=Intron
                     /label=Intron(2)
     misc_feature    complement(1199..1241)
                     /locus_tag="Intron(1)"
                     /label=Intron
                     /label=Intron(1)
     misc_feature    complement(1358..1452)
                     /locus_tag="Intron"
                     /label=Intron
     misc_feature    2498..2531
                     /locus_tag="Lox2272"
                     /label=Lox2272
     misc_feature    2532..2553
                     /locus_tag="GFP w/ syntrons"
                     /label=GFP w/ syntrons
     misc_feature    2532..2553
                     /locus_tag="syntron3"
                     /label=syntron3
     misc_feature    2551..2553
                     /locus_tag="Sap Trap gg Overlap"
                     /label=Sap Trap gg Overlap
     primer_bind     2554..2574
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(2554..2573)
                     /locus_tag="oMLS633"
                     /label=oMLS633
     primer_bind     2555..2574
                     /locus_tag="oMLS028"
                     /label=oMLS028
     primer_bind     complement(2568..2587)
                     /locus_tag="oMLS790"
                     /label=oMLS790
     misc_recomb     2590..2596
                     /locus_tag="attR4"
                     /label=attR4
     primer_bind     2591..2596
                     /locus_tag="oMLS142"
                     /label=oMLS142
     promoter        2611..3114
                     /locus_tag="Pmex-5"
                     /label=Pmex-5
     primer_bind     2611..2630
                     /locus_tag="Forward primer"
                     /label=Forward primer
     primer_bind     complement(3094..3114)
                     /locus_tag="reverse primer"
                     /label=reverse primer
     CDS             3141..3143
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and
     misc_feature    3144..3182
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     CDS             3183..4103
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(5)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(5)
     CDS             5407..6384
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(1)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(1)
     CDS             8283..9288
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(2)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(2)
     intron          8460..8510
                     /locus_tag="intron sequence"
                     /label=intron sequence
     CDS             10676..11397
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(3)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(3)
     misc_feature    11109..11114
                     /locus_tag="BbsI"
                     /label=BbsI
     CDS             12597..13124
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(4)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(4)
     misc_feature    13125..13157
                     /locus_tag="Flexible Linker"
                     /label=Flexible Linker
     misc_feature    13158..13160
                     /locus_tag="NaeI"
                     /label=NaeI
     misc_feature    13161..13163
                     /locus_tag="NaeI(1)"
                     /label=NaeI
                     /label=NaeI(1)
     misc_feature    13164..13247
                     /locus_tag="egl-13 NLS"
                     /label=egl-13 NLS
     misc_feature    13248..13250
                     /locus_tag="Stop"
                     /label=Stop
     misc_recomb     complement(13262..13275)
                     /locus_tag="AttR2"
                     /label=AttR2
     3'UTR           13276..13607
                     /locus_tag="tbb-2 utr"
                     /label=tbb-2 utr
     misc_feature    13278..13280
                     /locus_tag="Stop(1)"
                     /label=Stop
                     /label=Stop(1)
     misc_recomb     complement(13608..13614)
                     /locus_tag="attL3"
                     /label=attL3
     misc_recomb     complement(13615..13628)
                     /locus_tag="attR3"
                     /label=attR3
     primer_bind     13636..13653
                     /locus_tag="oMLS632"
                     /label=oMLS632
     primer_bind     complement(13636..13653)
                     /locus_tag="oMLS027"
                     /label=oMLS027
     primer_bind     complement(13636..13653)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    13703..13999
                     /locus_tag="Mos1 drosophila(1)"
                     /label=Mos1 drosophila
                     /label=Mos1 drosophila(1)
     CDS             14083..14151
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             14788..15447
                     /locus_tag="AmpR"
                     /label=AmpR
     primer_bind     complement(15459..15478)
                     /locus_tag="oMLS1008"
                     /label=oMLS1008
     primer_bind     15513..15532
                     /locus_tag="oMLS1009"
                     /label=oMLS1009
     rep_origin      15545..16227
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgcac cgtacgtctc
       61 gaggaattct atcaggtgta caagtatgaa atgtcgtttc gaacatatag atgtctcgca
      121 aacgtaaata tttatcgatt gtcataaaac tttgaccttg tgaagtgtca accttgactg
      181 tcgaaccacc atagtttggc gcgaattgag cgtcataatt gtttactctc agtgcagtca
      241 acatgtcgag tttcgtgccg aataaagagc aaacgcggac agtattaatt ttctgttttc
      301 atttgaagaa aacagctgcg gcagtttaaa catgatttta ctaacataac ttcgtataaa
      361 gtatcctata cgaagttatg acattctcta atgaaaaaat ctttcagttg aaattgaaaa
      421 tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg tttttagcga
      481 gtgtgacagg acagcgaaaa aatatagaaa caagggggga actgaaaagc ttaggaatgc
      541 attgaacatg agaaggggaa ggggaaggaa caaactagac aggaattatt ggaatttaat
      601 cacatttgga gttttttttc tattcgacag aataattatc cagaacattt ttgtattaaa
      661 tatttatgca tcatatgagt agtcggcttt gttgtgcatg acgagtttgt tatcgacgaa
      721 atagaagctg tcagaacgag tctcgtttgg attgttgatc atgtcgtcca ctgaaaaaga
      781 gattagtctt ttgaattgta ctttttagaa taatgactca ctgagttgtt gagagagttg
      841 agggaactca tagatatgtt cacagttgtt tcgtgaattc ggaatacaga atccgaattc
      901 aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga cgttcgatca ttcggaaatg
      961 atggattggg atgtctccta ctttgaattc cacagttgct ccgaccgttt tgagtttcaa
     1021 aaagtttgga gcaaatctat aacgcacgta tcttgccgac tcctgtggcg actcatcatt
     1081 ctcctgatcg ttctccggtt tggcgatctc gaaaagcact tgctcagtgt ccagatcacg
     1141 gatttggaac ttggtgaact cgatgttata gatgttcgca gatggggagc ataagaatcc
     1201 taaatttatg ttttaaactg aaatccaaag ggagcaagat accttgagtg attcccggaa
     1261 gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc aagctccgat tccgttgtga
     1321 ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa atatggaaaa gttcaacaaa
     1381 aagaaaagag aaaagaatga aatcggatat caagagttag ttgagcggtt tctctagttt
     1441 tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt ggaatcgatt gttgttgctc
     1501 ggctttcata tcggtttggt tggaagcggc tgaaaacgga aagaagtgga agaaggaaaa
     1561 gagtgtggtg tgacaggaaa atggtaatta gagggtgcca aataaccagc tatattttgt
     1621 ttttttttga aaacattttt aaaaagaaaa atacgataat gatatcagat ggatttccgg
     1681 aaaactggta tgaaaaattt caaccttttt gagtacatgt aatcaaaata cactttgtaa
     1741 attatcattt ttattgaaac tccaccattt ttctatttat aacgctaata atttgaaaaa
     1801 gaaacctgtt gcgaaccgcg gggtgaatcc caaaaacgaa tgcgttttgg tggagtgatt
     1861 gattcgaatc gaagaagaaa aagaagaaga cgtggaatag agagctcact cttaaccgag
     1921 cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg tcttcttctt cttcttcttc
     1981 gaatgattga cagaaatggg aaaaagagga agattgagaa gggaaaaagg aaggagaaaa
     2041 gaagcagaag aagacgtcag agaggagagg aacgagcgga aaagcagcgg gcgcaagtca
     2101 tagaagtagc agagctgggg agaagaagac actatccaag aaaggaatga cgagagagta
     2161 tgcaaagggg tatagggtgc agacagaata ggaacagaat aacagatgat gagccaagaa
     2221 gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt gggtcaattt gagcatgatg
     2281 aattgaaatc atcccttgtt gggagttaat aaccggtttg ttatcagaaa ccctgtaata
     2341 gaagggcgcc ctaactttga gccaattcat cccggtttct gtcaaatata tcaaaaagtg
     2401 gtcaactgac aaattgtttt tgatattata ataaacattt tatccgttaa caattttcga
     2461 atacttttta caaggacttg gataaattgg ctcaaagata acttcgtata aagtatccta
     2521 tacgaagtta ttaactaatc tgatttaaat tttcaggaaa cagctatgac catgattacg
     2581 ccaagctatc aactttgtat agaaaagttg ggttttcaca acggcaaaat atcagttttt
     2641 aaaaaattaa accataaaac aaataatata acccaatttt tacatcaaac cacaagaaaa
     2701 aaatacattt gggcccacgg ataaagaaat taaaaaaata cattttttaa aggcgcaccg
     2761 aattaaaatt catttgggtc ttaccgcgta taccgtactc cgtttgtttg atcatttttg
     2821 tcagcgctgg cggttgtttt ttcatttcat ttctgcttca aagacgtttt ctcgaataat
     2881 ttttcgttta ttctcttttt taaaattaat ttctagccgt aaatgttata aattcaccca
     2941 tttaacgcaa atttcatggt aatctcatgg aaaaatgcag tttctttgtt aaagaaagct
     3001 taaatagcaa aaattccccg actttcccca aaatcctgct cgattttccg ttttctcatt
     3061 gtattctctc ttaattaatt ttatcgataa tcaattgaat gtttcagaca gagacaagtt
     3121 tgtacaaaaa agcaggctcg atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     3181 aaatggacaa gaagtactcc atcggactcg acatcggaac caactccgtc ggatgggccg
     3241 tcatcaccga cgagtacaag gtcccatcca agaagttcaa ggtcctcgga aacaccgacc
     3301 gtcactccat caagaagaac ctcatcggag ccctcctctt cgactccgga gagaccgccg
     3361 aggccacccg tctcaagcgt accgcccgtc gtcgttacac ccgtcgtaag aaccgtatct
     3421 gctacctcca ggagatcttc tccaacgaga tggccaaggt cgacgactcc ttcttccacc
     3481 gtctcgagga gtccttcctc gtcgaggagg acaagaagca cgagcgtcac ccaatcttcg
     3541 gaaacatcgt cgacgaggtc gcctaccacg agaagtaccc aaccatctac cacctccgta
     3601 agaagctcgt cgactccacc gacaaggccg acctccgtct catctacctc gccctcgccc
     3661 acatgatcaa gttccgtgga cacttcctca tcgagggaga cctcaaccca gacaactccg
     3721 acgtcgacaa gctcttcatc cagctcgtcc agacctacaa ccagctcttc gaggagaacc
     3781 caatcaacgc ctccggagtc gacgccaagg ccatcctctc cgcccgtctc tccaagtccc
     3841 gtcgtctcga gaacctcatc gcccagctcc caggagagaa gaagaacgga ctcttcggaa
     3901 acctcatcgc cctctccctc ggactcaccc caaacttcaa gtccaacttc gacctcgccg
     3961 aggacgccaa gctccagctc tccaaggaca cctacgacga cgacctcgac aacctcctcg
     4021 cccagatcgg agaccagtac gccgacctct tcctcgccgc caagaacctc tccgacgcca
     4081 tcctcctctc cgacatcctc cgtgtaagga gttgaacggc tgaaaaatcg atattttgag
     4141 cgaaaaaagc cggaaaaatg gattttatcg gataaaattt gattttttga gctgagaaat
     4201 gcctttatag acgaatttcc ttgtcaaatt gttaaaaact gcaaaatttg ccaaaaaaaa
     4261 agcatttttt atattttttt ttacaaaaaa ttccacaaaa atggcaaaat ttgactaaaa
     4321 atgccaattt tgtcgttttc tccgtgccac agcggccgaa aatcgatttt taagcgattt
     4381 tcgggtgaaa aagtgtaaaa accgactgaa aatccagctg aaaacgacaa aaacggtaag
     4441 ttttagctaa ttttcacctt tttttcgata tttttttact aaaaaaaaac cgaaaaattg
     4501 agtttttttc aaatttctgc aaaaattctg caattttagc aatttccata ctattttgac
     4561 gccgaaaaag cttaaaaata tgatttttag aggtttttaa tggaaaattc atggattttt
     4621 caaatttttc cacgaaaatc attgaaaaat ttgaaaaaat tgatttccta ttcaaagttc
     4681 tagctaaaaa ctgcaatttt agcaatttcc atactatttt agtactgaaa aagcctaaaa
     4741 atgtgatttt cagaggattt caatggaaaa ttcatggatt tttccaattt ttcaatgaaa
     4801 accattgaaa aaccctggaa aattgagttt ttcttcaaag tttaggaaat ttatgagaat
     4861 ttttgaataa aaaattttta aatttgaaaa aaattgaaat tttaattcta aattttaaag
     4921 aaagttgatt ttttaataat tttttttttt gaataaaaaa aaatcaactt tctttaaaat
     4981 ttagaattaa aatttcaatt tttttattca aaaattctca taaatttcgt aaacttttaa
     5041 taaaaactca atttttccga tttttttcga tgattttcac taaaaaattg gaaaaattca
     5101 tcaatttcct attgaaaacc tctaaaaatt atattttttt tgcttttccg acgtcaaaat
     5161 agtatggaaa atgctaaaat tgcagaattt ttccagaaat ttgaagaaaa aactcaattt
     5221 tttccgtttt ttcaatgatt ttcatgaaaa aattggaaaa ttttgtaaat tttccattga
     5281 aaacctctga aaatttggaa attttcgaat ttaaatgttt aaaaaaaatt gaatattttg
     5341 cgagtttttg aatttttttt gagaattttt gaattttttt cacccaaaat tttttcaatt
     5401 tttcaggtca acaccgagat caccaaggcc ccactctccg cctccatgat caagcgttac
     5461 gacgagcacc accaggacct caccctcctc aaggccctcg tccgtcagca gctcccagag
     5521 aagtacaagg agatcttctt cgaccagtcc aagaacggat acgccggata catcgacgga
     5581 ggagcctccc aggaggagtt ctacaagttc atcaagccaa tcctcgagaa gatggacgga
     5641 accgaggagc tcctcgtcaa gctcaaccgt gaggacctcc tccgtaagca gcgtaccttc
     5701 gacaacggat ccatcccaca ccagatccac ctcggagagc tccacgccat cctccgtcgt
     5761 caggaggact tctacccatt cctcaaggac aaccgtgaga agatcgagaa gatcctcacc
     5821 ttccgtatcc catactacgt cggaccactc gcccgtggaa actcccgttt cgcctggatg
     5881 acccgtaagt ccgaggagac catcacccca tggaacttcg aggaggtcgt cgacaaggga
     5941 gcctccgccc agtccttcat cgagcgtatg accaacttcg acaagaacct cccaaacgag
     6001 aaggtcctcc caaagcactc cctcctctac gagtacttca ccgtctacaa cgagctcacc
     6061 aaggtcaagt acgtcaccga gggaatgcgt aagccagcct tcctctccgg agagcagaag
     6121 aaggccatcg tcgacctcct cttcaagacc aaccgtaagg tcaccgtcaa gcagctcaag
     6181 gaggactact tcaagaagat cgagtgcttc gactccgtcg agatctccgg agtcgaggac
     6241 cgtttcaacg cctccctcgg aacctaccac gacctcctca agatcatcaa ggacaaggac
     6301 ttcctcgaca acgaggagaa cgaggacatc ctcgaggaca tcgtcctcac cctcaccctc
     6361 ttcgaggacc gtgagatgat cgaggtgctg aagctcgaaa atttggagct tttacacgag
     6421 atttttgaag aaaaagcctg aaaatcgaga gattttgagc tgtggaattc gttgaaatta
     6481 cataattcgc gcccgtaatt ctttaaaaaa acgctgaaat ttgcagattt tgccagaaag
     6541 ttgaagaaaa atctactttt ccggcattct ctattatttt catggaaaat tagaaaaatc
     6601 catgaataaa cagtgaaaat tgctgatttt acctgaaatt ttgaaaaaaa aaaaaatcta
     6661 aaatcaaggt tttaagccta aaaactgcag atttcagcca aaattgtgaa aaaagctgat
     6721 tttttgctat tttgagcttt tccatggggt ggaaattgcc aaaaattacg gaacattcac
     6781 acaaatttgc tgaataatcc aattttccag tgttttctag agtttccgta gaaattttga
     6841 aaaaacccag taaattttca atcggaaaac ctctaaaaac atcgtttcag gcctataaat
     6901 agtaaaaatt accgatttta ttcgaaaatt taaggaaaag tcgtgaaaaa cgagatttta
     6961 agcctcaaaa ctgcagattc tatctaaaat taaaaaaaaa aaacataaat atccgaattt
     7021 ttggaggaaa agcctgaaaa tcgatatttt aagcctcaaa actgaagatt tcagccaaaa
     7081 aaaaaaacag atttttcgct attttatgtg ccaaaaaacc acattccgag cttttccaga
     7141 gtggaaattg ccaaaaatta cagaattttc ccagaaatta gcaaaaaaaa aacccatttt
     7201 tcaggcgttt tcaacgattt tcatagcaaa tttgaaaaat ccatgaattt tttaattata
     7261 gaaaaccctc tgaaaattgc agttttaagc tattccagca ttcaaatagt atggaaattg
     7321 ctaaaaattg aagaattttc ccaaaaattt gatgaaaact tatttttctg gcgttttcga
     7381 tgattttcat ggaatttcga gaaaattaat gaattttcaa ttaaaaaacg tctgcaaaat
     7441 cacatttttt aaagcttttt cagcaacaaa atagtatgga aattgctttt taaaaaagcg
     7501 gaatttttgg aaaaaaggtt aaattttcag tcagtttttg cactttttga ctgaaaattc
     7561 actgaaatta gggaaaaatt ccggaaaaat gattttttcg aattgtaggg caaattttgc
     7621 aatttcaagc aatttccata atattttaat gctggaaaag ctcaaattta cgttttaatg
     7681 ggattttagt gaaaaattca tggattttcc gaaaaatttg caggaaaatc atagtaaaga
     7741 cctcaaaaaa tcaataaaaa atttaaaaaa ttggacaatc acatttctgc gtaaaatctg
     7801 cactttttca gtaaaaaata ccgattttcc gtgaaaattc tcgattttta atcgaaaatc
     7861 tcgaaatttt tacaaatttc agcaccaaaa tcgttgaaaa ttctgaaaat ttgaattttt
     7921 tttccttgtt ttttgagtaa aatttgcact ttttcagtaa agaataccga ttttccgtga
     7981 aaattctcgg tttttaatcg aaaatctcga aatttttaca aatttcagct ccgaaatagc
     8041 tgaaaattgg acaaaaattt caatttttgc cctgtttttt gagtaaaatc tgcacttttt
     8101 agttgaaaaa aaacacggat tttgtaggaa aatttgggaa aaatctccaa attttcacaa
     8161 ttttccagta aaaacagcaa tttttaaaac ggtttttaat caaaaatctt tagaattttc
     8221 caaatttcag caccaaaaaa gctaaaaatc gctgaaattt tcgacaaatt ccaaatttcc
     8281 aggagcgtct caagacctac gcccacctct tcgacgacaa ggtcatgaag cagctcaagc
     8341 gtcgtcgtta caccggatgg ggacgtctct cccgtaagct catcaacgga atccgtgaca
     8401 agcagtccgg aaagaccatc ctcgacttcc tcaagtccga cggattcgcc aaccgtaacg
     8461 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag ttcatgcagc
     8521 tcatccacga cgactccctc accttcaagg aggacatcca gaaggcccag gtctccggac
     8581 agggagactc cctccacgag cacatcgcca acctcgccgg atccccagcc atcaagaagg
     8641 gaatcctcca gaccgtcaag gtcgtcgacg agctcgtcaa ggtcatggga cgtcacaagc
     8701 cagagaacat cgtcatcgag atggcccgtg agaaccagac cacccagaag ggacagaaga
     8761 actcccgtga gcgtatgaag cgtatcgagg agggaatcaa ggagctcgga tcccagatcc
     8821 tcaaggagca cccagtcgag aacacccagc tccagaacga gaagctctac ctctactacc
     8881 tccagaacgg acgtgacatg tacgtcgacc aggagctcga catcaaccgt ctctccgact
     8941 acgacgtcga ccacatcgtc ccacagtcct tcctcaagga cgactccatc gacaacaagg
     9001 tcctcacccg ttccgacaag aaccgtggaa agtccgacaa cgtcccatcc gaggaggtcg
     9061 tcaagaagat gaagaactac tggcgtcagc tcctcaacgc caagctcatc acccagcgta
     9121 agttcgacaa cctcaccaag gccgagcgtg gaggactctc cgagctcgac aaggccggat
     9181 tcatcaagcg tcagctcgtc gagacccgtc agatcaccaa gcacgtcgcc cagatcctcg
     9241 actcccgtat gaacaccaag tacgacgaga acgacaagct catccgtggt tggtttttcc
     9301 gagagaaaaa cgctgaaaaa tgccgaaaat tttgaatttg caggcttttt aggtgcaaaa
     9361 gtacggaaaa atcggaaaaa aatcaggaaa acatggggaa aatccaattt tccagaaaaa
     9421 atgtttgaaa aaattgaaaa cttttgtttt tttagtaata aagttttaaa aaatggcaaa
     9481 aatagctgaa aacagcgaaa aaaaaaaatg tttttttcag gaaaaaattt gcaaaaaaat
     9541 gaagagcaac aataattccc ttttttttct gtgttttttt cggaaaaatt gagatcgaaa
     9601 acgaaaaaca gcgaaaattc ccaattttcc cagtttttaa aacatttttt tgtaaaaatt
     9661 tggaaaaaac gagttttttc agaaaaaaat gtgaaaaaaa aggtaaatta ccaatttatg
     9721 cttttttttc gatcgagtaa aaaatcgatt tttccaagat ttttcgcgaa aaattcaaaa
     9781 aaaaagaacg aaaattctcg attttctact ttttttcgaa aaaaaaagga aattatagat
     9841 ttttccaaga tttttcgcga aaaaattcga aaaaaatgtt tttttcgaaa atttcagatt
     9901 ttctaatttt ttcagaagaa ttcgaaaaaa aaaatcaaaa aattcgcaat ttttagattt
     9961 ttttaaaatc tcaattttct ataattattt tcctaaaaaa atgtaattaa aaaaccgcta
    10021 aaaacggtaa attccaaatt tttgcatttt ttcaaaaaaa atttgaaaaa aaaaatcaga
    10081 aaattctcaa atctattccc tttttttaaa atttttccca aaccaaaaaa aaaaacggta
    10141 aattttcaaa tttagcattt ttccaaaatt aaacataaaa aaaaacgcaa atttccaaat
    10201 ttcgtttttt tttcaaaaaa gttgaaaaat ccagaaaata ccctcttgtc catgaaaaaa
    10261 tacgaaaatt ttcaataaaa aatcaatttc ttcttaaaaa attaattctc gatttttcca
    10321 tgaaaaaaaa tcaaatatta attaaatttg aaaaaaaaaa gaatttatct aatagtttcg
    10381 ttttttttca gaggaaaaat ttgaaaaaaa aaacagtaaa ttctctttct ttttgaaaaa
    10441 aaaaattatc gattcttcca agttttttcc cgaaaaaatc gacaaaaaaa cgagaatttc
    10501 cgattttcta tttttttttt caaaaaaatg tgaaaaaaaa aattaaaaaa ttccaatttt
    10561 gcattttttt ttcaaaaaaa atttaaaaac accgtataat tcccgatttt tccatgaaaa
    10621 aatacgaaaa attccaataa aaatcaacta ttttaaaaat ttgccgtttt ttcagaggtc
    10681 aaggtcatca ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccagttctac
    10741 aaggtccgtg agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc
    10801 ggaaccgccc tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac
    10861 aaggtctacg acgtccgtaa gatgatcgcc aagtccgagc aggagatcgg aaaggccacc
    10921 gccaagtact tcttctactc caacatcatg aacttcttca agaccgagat caccctcgcc
    10981 aacggagaga tccgtaagcg tccactcatc gagaccaacg gagagaccgg agagatcgtc
    11041 tgggacaagg gacgtgactt cgccaccgtc cgtaaggtcc tctccatgcc acaggtcaac
    11101 atcgtcaaga agaccgaggt ccagaccgga ggattctcca aggagtccat cctcccaaag
    11161 cgtaactccg acaagctcat cgcccgtaag aaggactggg acccaaagaa gtacggagga
    11221 ttcgactccc caaccgtcgc ctactccgtc ctcgtcgtcg ccaaggtcga gaagggaaag
    11281 tccaagaagc tcaagtccgt caaggagctc ctcggaatca ccatcatgga gcgttcctcc
    11341 ttcgagaaga acccaatcga cttcctcgag gccaagggat acaaggaggt caagaaggtg
    11401 cttacaattt tctggagaaa aatattttaa aaaataacga aaatggaccg aatttttgga
    11461 gaaaatttta aaaaaaacga catttttccc aaaaaattga aaaatttccg aaaaaacgtt
    11521 caaaattttc aaaaaaggag catttttcga aaaaaaaaac gataatacgg aaattttttg
    11581 attttccagt ttttttctca aagaaaatcg gaaaaaggtt ttttttcttt aaaaacgtcc
    11641 aaaatttttc gaaaaaagag aaaattttcg attttccgat ttttttcaga aaaaaattga
    11701 aaaaaaaaga aattttccga ttttctcaga gaaagacgaa aagacgatat ttttcggaaa
    11761 aaagttgaaa aatcaaaaaa aaaaccgaaa aacggccgaa attttctaaa aattggaaat
    11821 attgtcgatt tttcgaaaaa aaagagtcaa aagttttcaa aaaaatatat tttttcgatt
    11881 ttccgttttt ttttttcagt aaacattgaa acaaacgaaa tttttccaaa aaaatccaat
    11941 tattttccca aatatcgaaa tatttttgat tttccgtttt tttcagaaaa aagttaaaaa
    12001 aaaaagggaa aattaagatt ttcctgattt tttttgagaa aaaatcgata aaaattgaat
    12061 attctggaaa aaacctttaa aaaattcgaa ttaaatcgaa aaacgtccaa aattttctaa
    12121 aaaattgaaa ttttttttga aaaaaaacgg aaaaatttag atttcccgac ttttttcaat
    12181 gaaaaatcga attttcgttg acttttcatt aaaaaatcca aacgcatttt tttccactaa
    12241 aatctcgaaa aagtgtcata ttgcaccata ttgtttccac attcatatta ttttgagcta
    12301 aatcaggtga ttttctgtat tttgatacta atttctattt ttagaaaaaa tgtgggaaca
    12361 atatggtgca atatgacatt ttttcgagat tttagtggag aaaaaatgcg tttggaaacg
    12421 aattttataa taaaaagtca acgaaaattc gatttttcat ggaaaaatag ggaaatcgaa
    12481 aacaaatttg cttttttttc aaaaaaaaaa acggaaattc gaaatttttc cgttctttca
    12541 aaaaaaaaat cgattttttt ggaaattttt tttttcaaat ttttaaaaat ttccaggacc
    12601 tcatcatcaa gctcccaaag tactccctct tcgagctcga gaacggacgt aagcgtatgc
    12661 tcgcctccgc cggagagctc cagaagggaa acgagctcgc cctcccatcc aagtacgtca
    12721 acttcctcta cctcgcctcc cactacgaga agctcaaggg atccccagag gacaacgagc
    12781 agaagcagct cttcgtcgag cagcacaagc actacctcga cgagatcatc gagcagatct
    12841 ccgagttctc caagcgtgtc atcctcgccg acgccaacct cgacaaggtc ctctccgcct
    12901 acaacaagca ccgtgacaag ccaatccgtg agcaggccga gaacatcatc cacctcttca
    12961 ccctcaccaa cctcggagcc ccagccgcct tcaagtactt cgacaccacc atcgaccgta
    13021 agcgttacac ctccaccaag gaggtcctcg acgccaccct catccaccag tccatcaccg
    13081 gactctacga gacccgtatc gacctctccc agctcggagg agactctgga ggatctggag
    13141 gaactggagg atcttccgcc ggcgggagct ctatgagccg tagacgaaaa gcgaatccga
    13201 caaaactgag tgaaaacgcg aagaagcttg ccaaggaagt tgaaaattaa cacccagctt
    13261 tcttgtacaa agtgggataa atgcaaaatc ctttcaagca ttcccttctt ctctatcact
    13321 cttctttctt tttgtcaaaa aattctctcg ctaatttatt tgctttttta atgttattat
    13381 tttatgactt tttatagtca ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa
    13441 tgtgattctg tctgatgtac tttcacaatc tctcttcaat tccattttga agtgctttaa
    13501 acccgaaagg ttgagaaaaa tgcgagcgct caaatatttg tattgtgttc gttgagtgac
    13561 ccaacaaaaa gaggaaactt tattgtgccg ccaagaaaaa agtctcacaa ctttattata
    13621 catagttgat aattcactgg ccgtcgtttt acatggcacg cccttctatt acagggtttc
    13681 tgataacaaa gggtgccgga tctgcggctt actcaccaga cctggcccca tccgattacc
    13741 acctattcgc ttcgatggga cacgcactcg ctgagcagcg cttcgattct tacgaaagtg
    13801 tgaaaaaatg gctcgatgaa tggttcgccg caaaagacga tgagttctac tggcgtggaa
    13861 tccacaaatt gcccgagaga tgggaaaaat gtgtagctag cgacggcaaa tactttgaat
    13921 aaatgatttt ttctttttcc acaaaattta acgtgttttt tgatttaaaa aaaacgacat
    13981 ttcatacttg tacacctgat atctagatac ctaggtgagc tctggtaccc ccctggcgtt
    14041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    14101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
    14161 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
    14221 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    14281 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    14341 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    14401 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    14461 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    14521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    14581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    14641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    14701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    14761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    14821 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    14881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    14941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    15001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    15061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    15121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    15181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    15241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    15301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    15361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    15421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    15481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    15541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    15601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    15661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    15721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    15781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    15841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    15901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    15961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    16021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    16081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    16141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    16201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt
    16261 gctcacatgt tctttcctgc gttatcccc
//
