LOCUS       pMLS715                 6670 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..112
                     /locus_tag="oMLS1462"
                     /label=oMLS1462
     misc_feature    87..114
                     /locus_tag="Artificial 3'ITR"
                     /label=Artificial 3'ITR
     misc_feature    115..200
                     /locus_tag="Mos1 drosophila"
                     /label=Mos1 drosophila
     primer_bind     complement(180..218)
                     /locus_tag="oMLS1463"
                     /label=oMLS1463
     primer_bind     201..218
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     226..350
                     /locus_tag="attR3"
                     /label=attR3
     CDS             459..1115
                     /locus_tag="CmR"
                     /label=CmR
     primer_bind     complement(1315..1325)
                     /locus_tag="oMLS1457(1)"
                     /label=oMLS1457
                     /label=oMLS1457(1)
     CDS             complement(1460..1765)
                     /locus_tag="ccdB"
                     /label=ccdB
     misc_recomb     complement(1805..1929)
                     /locus_tag="attR4 recomb"
                     /label=attR4 recomb
     primer_bind     complement(1945..1965)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    complement(2038..2045)
                     /locus_tag="PmeI"
                     /label=PmeI
     misc_feature    complement(2056..2058)
                     /locus_tag="AfeI"
                     /label=AfeI
     misc_feature    2059..2064
                     /locus_tag="GFP w/ syntrons(1)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(1)
     misc_feature    2059..2064
                     /locus_tag="syntron3(2)"
                     /label=syntron3
                     /label=syntron3(2)
     misc_feature    2065..2084
                     /locus_tag="GFP w/ syntrons"
                     /label=GFP w/ syntrons
     misc_feature    2065..2084
                     /locus_tag="syntron3(1)"
                     /label=syntron3
                     /label=syntron3(1)
     misc_feature    2085..2118
                     /locus_tag="Lox2272"
                     /label=Lox2272
     gene            complement(2119..4236)
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label=C.Briggsae unc-119(+)
     CDS             complement(2403..3248)
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
     misc_feature    complement(2510..2560)
                     /locus_tag="Intron(2)"
                     /label=Intron
                     /label=Intron(2)
     misc_feature    complement(2938..2980)
                     /locus_tag="Intron"
                     /label=Intron
     misc_feature    complement(3097..3191)
                     /locus_tag="Intron(1)"
                     /label=Intron
                     /label=Intron(1)
     primer_bind     3551..3556
                     /locus_tag="oMLS1457"
                     /label=oMLS1457
     misc_feature    4237..4270
                     /locus_tag="Lox2272(1)"
                     /label=Lox2272
                     /label=Lox2272(1)
     primer_bind     4271..4314
                     /locus_tag="oMLS1464"
                     /label=oMLS1464
     misc_feature    4271..4289
                     /locus_tag="GFP w/ syntrons(2)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(2)
     misc_feature    4271..4289
                     /locus_tag="syntron3(3)"
                     /label=syntron3
                     /label=syntron3(3)
     misc_feature    4290..4292
                     /locus_tag="syntron3"
                     /label=syntron3
     misc_feature    4290..4292
                     /locus_tag="GFP w/ syntrons(3)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(3)
     misc_feature    4293..4414
                     /locus_tag="Mos1 drosophila(1)"
                     /label=Mos1 drosophila
                     /label=Mos1 drosophila(1)
     primer_bind     complement(4393..4435)
                     /locus_tag="oMLS1465"
                     /label=oMLS1465
     misc_feature    complement(4393..4414)
                     /locus_tag="oCF1210"
                     /label=oCF1210
     primer_bind     complement(4393..4414)
                     /locus_tag="oMLS1529"
                     /label=oMLS1529
     CDS             4485..4553
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     promoter        4873..4991
                     /locus_tag="bla promoter"
                     /label=bla promoter
     CDS             5190..5849
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      5997..6670
                     /locus_tag="pUC ori"
                     /label=pUC ori
     primer_bind     complement(6348..6353)
                     /locus_tag="oMLS1457(2)"
                     /label=oMLS1457
                     /label=oMLS1457(2)
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccagc ggataacaat ttcacatcag gtgtacaagt atgaaatgtc gtttcgaaca
      121 tatagatgtc tcgcaaacgt aaatatttat cgattgtcat aaaactttga ccttgtgaag
      181 tgtcaacctt gactgtcgaa tgtaaaacga cggccagtga attatcaact atgtataata
      241 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      301 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
      361 aggcaccccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      421 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      481 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      541 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      601 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      661 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      721 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      781 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      841 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
      901 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
      961 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1021 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1081 tacaacagta ctgcgatgag tggcagggcg gggcgtaatc tagaggatcc ggcttactaa
     1141 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1201 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     1261 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggttcg
     1321 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     1381 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgccgac
     1441 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     1501 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     1561 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     1621 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     1681 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     1741 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     1801 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     1861 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca acttttctat
     1921 acaaagttga tagcttggcg taatcatggt catagctgtt tcctgcctag gtgagctctg
     1981 gtaccctcta gtcaaggcct taagtgagtc gtattacgta gcccgggcct agttctagtt
     2041 taaaccacgt gcgtgagcgt aagtttaaac atgattttac taacataact tcgtataaag
     2101 tatcctatac gaagttatga cattctctaa tgaaaaaatc tttcagttga aattgaaaat
     2161 gagttaaagt tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag
     2221 tgtgacagga cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca
     2281 ttgaacatga gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc
     2341 acatttggag ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat
     2401 atttatgcat catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa
     2461 tagaagctgt cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag
     2521 attagtcttt tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga
     2581 gggaactcat agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca
     2641 aagtcaaaac acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga
     2701 tggattggga tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa
     2761 aagtttggag caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc
     2821 tcctgatcgt tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg
     2881 atttggaact tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct
     2941 aaatttatgt tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag
     3001 tgctaaaacg tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat
     3061 tccttgttcg gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa
     3121 agaaaagaga aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt
     3181 ctgagtctca cctgcgacgg gaaggtcgcc gagccgggtg gaatcgattg ttgttgctcg
     3241 gctttcatat cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag
     3301 agtgtggtgt gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt
     3361 tttttttgaa aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga
     3421 aaactggtat gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa
     3481 ttatcatttt tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag
     3541 aaacctgttg cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg
     3601 attcgaatcg aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc
     3661 agcacacacc gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg
     3721 aatgattgac agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag
     3781 aagcagaaga agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat
     3841 agaagtagca gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat
     3901 gcaaaggggt atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag
     3961 agttgaaaag ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga
     4021 attgaaatca tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag
     4081 aagggcgccc taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg
     4141 tcaactgaca aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa
     4201 tactttttac aaggacttgg ataaattggc tcaaagataa cttcgtataa agtatcctat
     4261 acgaagttat taactaatct gatttaaatt ttcgcgaatt gagcgtcata attgtttact
     4321 ctcagtgcag tcaacatgtc gagtttcgtg ccgaataaag agcaaacgcg gacagtatta
     4381 attttctgtt ttcatttgaa gaaaacagct gcggacgtcg tgactgggaa aaccctggcg
     4441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     4621 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4681 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4741 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4801 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4861 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt
     6661 ttgctcacat
//
