LOCUS       pMLS716                 7094 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..112
                     /locus_tag="oMLS1462"
                     /label=oMLS1462
     misc_feature    87..114
                     /locus_tag="Artificial 3'ITR"
                     /label=Artificial 3'ITR
     misc_feature    115..200
                     /locus_tag="Mos1 drosophila"
                     /label=Mos1 drosophila
     primer_bind     complement(180..218)
                     /locus_tag="oMLS1463"
                     /label=oMLS1463
     primer_bind     201..218
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     primer_bind     227..247
                     /locus_tag="T7"
                     /label=T7
     misc_feature    complement(300..302)
                     /locus_tag="GG overlap site"
                     /label=GG overlap site
     misc_feature    complement(303..359)
                     /locus_tag="Unc-119 hDS"
                     /label=Unc-119 hDS
     misc_feature    360..362
                     /locus_tag="Sap Trap gg Overlap(2)"
                     /label=Sap Trap gg Overlap
                     /label=Sap Trap gg Overlap(2)
     promoter        363..762
                     /locus_tag="hsp16-41 promoter"
                     /label=hsp16-41 promoter
     misc_feature    complement(763..1893)
                     /locus_tag="Cre"
                     /label=Cre
     misc_feature    complement(1179..1229)
                     /locus_tag="intron"
                     /label=intron
     misc_feature    complement(1601..1651)
                     /locus_tag="intron(1)"
                     /label=intron
                     /label=intron(1)
     misc_feature    complement(1894..1896)
                     /locus_tag="STOP"
                     /label=STOP
     3'UTR           join(1897..2103,2105..2228)
                     /locus_tag="tbb-2 3'UTR"
                     /label=tbb-2 3'UTR
     misc_feature    1899..1925
                     /locus_tag="oMLS1449"
                     /label=oMLS1449
     misc_feature    complement(2018..2040)
                     /locus_tag="oMLS903"
                     /label=oMLS903
     misc_feature    2229..2231
                     /locus_tag="Sap Trap gg Overlap(1)"
                     /label=Sap Trap gg Overlap
                     /label=Sap Trap gg Overlap(1)
     misc_feature    complement(2232..2288)
                     /locus_tag="Unc-119 hUS"
                     /label=Unc-119 hUS
     misc_feature    2289..2291
                     /locus_tag="Sap Trap gg Overlap"
                     /label=Sap Trap gg Overlap
     primer_bind     complement(2332..2351)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(2369..2389)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    complement(2462..2469)
                     /locus_tag="PmeI"
                     /label=PmeI
     misc_feature    complement(2480..2482)
                     /locus_tag="AfeI"
                     /label=AfeI
     misc_feature    2483..2488
                     /locus_tag="GFP w/ syntrons(1)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(1)
     misc_feature    2483..2488
                     /locus_tag="syntron3(2)"
                     /label=syntron3
                     /label=syntron3(2)
     misc_feature    2489..2508
                     /locus_tag="GFP w/ syntrons"
                     /label=GFP w/ syntrons
     misc_feature    2489..2508
                     /locus_tag="syntron3(1)"
                     /label=syntron3
                     /label=syntron3(1)
     misc_feature    2509..2542
                     /locus_tag="Lox2272"
                     /label=Lox2272
     gene            complement(2543..4660)
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label=C.Briggsae unc-119(+)
     misc_feature    complement(2727..2754)
                     /locus_tag="oMLS1276"
                     /label=oMLS1276
     CDS             complement(2827..3672)
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
     misc_feature    complement(2934..2984)
                     /locus_tag="Intron(2)"
                     /label=Intron
                     /label=Intron(2)
     misc_feature    complement(3362..3404)
                     /locus_tag="Intron"
                     /label=Intron
     misc_feature    complement(3521..3615)
                     /locus_tag="Intron(1)"
                     /label=Intron
                     /label=Intron(1)
     primer_bind     3975..3980
                     /locus_tag="oMLS1457"
                     /label=oMLS1457
     misc_feature    4661..4694
                     /locus_tag="Lox2272(1)"
                     /label=Lox2272
                     /label=Lox2272(1)
     primer_bind     4695..4738
                     /locus_tag="oMLS1464"
                     /label=oMLS1464
     misc_feature    4695..4713
                     /locus_tag="GFP w/ syntrons(2)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(2)
     misc_feature    4695..4713
                     /locus_tag="syntron3(3)"
                     /label=syntron3
                     /label=syntron3(3)
     misc_feature    4714..4716
                     /locus_tag="syntron3"
                     /label=syntron3
     misc_feature    4714..4716
                     /locus_tag="GFP w/ syntrons(3)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(3)
     misc_feature    4717..4838
                     /locus_tag="Mos1 drosophila(1)"
                     /label=Mos1 drosophila
                     /label=Mos1 drosophila(1)
     primer_bind     complement(4817..4859)
                     /locus_tag="oMLS1465"
                     /label=oMLS1465
     misc_feature    complement(4817..4838)
                     /locus_tag="oCF1210"
                     /label=oCF1210
     primer_bind     complement(4817..4838)
                     /locus_tag="oMLS1529"
                     /label=oMLS1529
     CDS             4909..4977
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     promoter        5297..5415
                     /locus_tag="bla promoter"
                     /label=bla promoter
     CDS             5614..6273
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      6421..7094
                     /locus_tag="pUC ori"
                     /label=pUC ori
     primer_bind     complement(6772..6777)
                     /locus_tag="oMLS1457(1)"
                     /label=oMLS1457
                     /label=oMLS1457(1)
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccagc ggataacaat ttcacatcag gtgtacaagt atgaaatgtc gtttcgaaca
      121 tatagatgtc tcgcaaacgt aaatatttat cgattgtcat aaaactttga ccttgtgaag
      181 tgtcaacctt gactgtcgaa tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      241 tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgatt
      301 acatcgccga ccttgaactc gaccgtcgcg ccgaccgtct ttaatttcag aaaattcggc
      361 gcgatcacca aaaacggaac gttgagctgg acggaaatag tggtaaagtg acatgattat
      421 agtttgaaga tttctaattt cacaattaga gcaaatgttg ttcggtattt attttcaacg
      481 gtatttatac tattttccac ctttttctag aacattcgag ctgcttgttg caaaaggagg
      541 gcgactcaca ttcggtacat ggaaaagtag tgtacacaat aaagagaccc agatacattt
      601 tccgtctgcg tctctttgca cccaccggga gtattttcaa acgaatgcat ctaggacctt
      661 ctagaacatt ctgtaaggct gcagaatgcg ggtatataag gaaagcgggc tcagaggaag
      721 ccaacacgct ttgttctagt gcatctaaaa aacttcgaaa atatgtcaaa tcttctcact
      781 gttcatcaaa atcttccagc acttccagtt gacgcgacaa gtgatgaagt tcgcaagaac
      841 ctcatggata tgttccgtga cagacaagcc ttctccgagc acacctggaa gatgcttctc
      901 tcagtgtgcc gttcttgggc tgcctggtgc aagcttaaca accgcaagtg gttcccagcc
      961 gagccagaag acgttcgtga ctacttgctc tacctccaag ctcgtggatt ggcggtgaag
     1021 acgatccaac aacatctcgg acagctcaat atgctccatc gccgatctgg acttccacgc
     1081 ccatcagatt ctaatgccgt atctctcgta atgagacgta tccgcaagga aaatgttgat
     1141 gctggagaga gagccaagca agccctcgcc ttcgaaaggt aagtttaaat attaatgtat
     1201 tcaatcctaa gacttgacga aattttcaga accgacttcg atcaggtccg ttctcttatg
     1261 gaaaattccg atcgttgcca ggacatccga aacctcgcct tcctcggaat tgcttacaac
     1321 acacttctcc gtatcgccga aattgcccgc attcgcgtca aggatattag tcgcacagac
     1381 ggaggaagaa tgctcatcca catcggacgt acaaaaacgc tggtctcgac tgccggagtc
     1441 gaaaaagcgc tctcccttgg agttaccaag ctcgttgagc gctggatttc ggtttccgga
     1501 gtcgccgatg atccaaacaa ctacctgttc tgtcgtgttc gcaagaacgg agttgctgcc
     1561 ccgagtgcaa catcacaact ctcgactcga gctcttgaag gtaagtttaa acaacatatt
     1621 agctaagaat aagctgaaaa taaattttca ggaatcttcg aagctaccca ccgtctcatc
     1681 tacggagcca aggatgattc cggacaacgt tacttggctt ggtctggaca ttccgcccgt
     1741 gtcggagctg cccgtgacat ggctcgcgct ggagtttcca tcccagagat tatgcaagcc
     1801 ggaggttgga ccaacgtcaa cattgtgatg aactatatta gaaacttgga ttctgaaact
     1861 ggcgccatgg ttcgtcttct cgaagacggt gattaagata aatgcaaaat cctttcaagc
     1921 attcccttct tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat
     1981 ttgctttttt aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga
     2041 gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa
     2101 ttctattttg aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt
     2161 gtattgtgtt cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa
     2221 aagtctcaca taaatcaata tctcacatat cttgccgatt cggcttgcgc ctgcagattc
     2281 tcttccgtcc aatcgaattg gccgccaccg cggtggagct ccagcttttg ttccctttag
     2341 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgc ctaggtgagc
     2401 tctggtaccc tctagtcaag gccttaagtg agtcgtatta cgtagcccgg gcctagttct
     2461 agtttaaacc acgtgcgtga gcgtaagttt aaacatgatt ttactaacat aacttcgtat
     2521 aaagtatcct atacgaagtt atgacattct ctaatgaaaa aatctttcag ttgaaattga
     2581 aaatgagtta aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag
     2641 cgagtgtgac aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa
     2701 tgcattgaac atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt
     2761 aatcacattt ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt
     2821 aaatatttat gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac
     2881 gaaatagaag ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa
     2941 agagattagt cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag
     3001 ttgagggaac tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa
     3061 ttcaaagtca aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa
     3121 atgatggatt gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt
     3181 caaaaagttt ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc
     3241 attctcctga tcgttctccg gtttggcgat ctcgaaaagc acttgctcag tgtccagatc
     3301 acggatttgg aacttggtga actcgatgtt atagatgttc gcagatgggg agcataagaa
     3361 tcctaaattt atgttttaaa ctgaaatcca aagggagcaa gataccttga gtgattcccg
     3421 gaagtgctaa aacgtcgttc ggagtgattt gagctttctt cgcaagctcc gattccgttg
     3481 tgattccttg ttcggtgctt ggtggtggcc gtggcatctg gaaatatgga aaagttcaac
     3541 aaaaagaaaa gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag
     3601 ttttctgagt ctcacctgcg acgggaaggt cgccgagccg ggtggaatcg attgttgttg
     3661 ctcggctttc atatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga
     3721 aaagagtgtg gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt
     3781 tgtttttttt tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc
     3841 cggaaaactg gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg
     3901 taaattatca tttttattga aactccacca tttttctatt tataacgcta ataatttgaa
     3961 aaagaaacct gttgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg
     4021 attgattcga atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc
     4081 gagcagcaca caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc
     4141 ttcgaatgat tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga
     4201 aaagaagcag aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag
     4261 tcatagaagt agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga
     4321 gtatgcaaag gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa
     4381 gaagagttga aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg
     4441 atgaattgaa atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta
     4501 atagaagggc gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa
     4561 gtggtcaact gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt
     4621 cgaatacttt ttacaaggac ttggataaat tggctcaaag ataacttcgt ataaagtatc
     4681 ctatacgaag ttattaacta atctgattta aattttcgcg aattgagcgt cataattgtt
     4741 tactctcagt gcagtcaaca tgtcgagttt cgtgccgaat aaagagcaaa cgcggacagt
     4801 attaattttc tgttttcatt tgaagaaaac agctgcggac gtcgtgactg ggaaaaccct
     4861 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4921 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     4981 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     5041 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5101 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5161 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5221 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5281 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5341 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5401 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5461 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5521 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5581 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5641 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5701 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5761 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5821 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5881 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5941 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6001 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6061 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6121 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6181 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6241 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6301 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6361 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6421 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6481 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6541 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6601 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6661 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6721 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6781 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6841 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6901 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6961 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7021 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     7081 ccttttgctc acat
//
