LOCUS       pMLS485                13676 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        402..1010
                     /locus_tag="eft-3 promoter"
                     /label=eft-3 promoter
     misc_recomb     1011..1031
                     /locus_tag="attB1(1)"
                     /label=attB1
                     /label=attB1(1)
     misc_recomb     1018..1031
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    1018..1024
                     /locus_tag="Gateway recomb1"
                     /label=Gateway recomb1
     CDS             1037..1957
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and
     CDS             3261..4238
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(1)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(1)
     CDS             6137..7142
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(2)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(2)
     intron          6314..6364
                     /locus_tag="intron sequence"
                     /label=intron sequence
     CDS             8530..9251
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(3)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(3)
     misc_feature    8963..8968
                     /locus_tag="BbsI"
                     /label=BbsI
     CDS             10451..10978
                     /locus_tag="Cas9 open reading frame with interveningintron 
                     and(4)"
                     /label=Cas9 open reading frame with intervening
                     intron...
                     /label=Cas9 open reading frame with intervening intron
                     and(4)
     misc_feature    10979..11011
                     /locus_tag="Flexible Linker"
                     /label=Flexible Linker
     misc_feature    11012..11014
                     /locus_tag="NaeI(1)"
                     /label=NaeI
                     /label=NaeI(1)
     misc_feature    11015..11017
                     /locus_tag="NaeI"
                     /label=NaeI
     misc_feature    11018..11101
                     /locus_tag="egl-13 NLS"
                     /label=egl-13 NLS
     misc_feature    11102..11104
                     /locus_tag="Stop"
                     /label=Stop
     3'UTR           11105..11436
                     /locus_tag="tbb-2 3'UTR"
                     /label=tbb-2 3'UTR
     primer_bind     complement(11451..11471)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(11477..11499)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(11839..12521)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      11842..12456
                     /locus_tag="pMB1 Replication origin"
                     /label=pMB1 Replication origin
     misc_feature    12616..13476
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(12619..13278)
                     /locus_tag="AmpR(1)"
                     /label=AmpR
                     /label=AmpR(1)
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgcacctttg gtcttttatt
      421 gtcaacttcc attggttctt ccattgtttc tgttaaatta atgaattttt cataaaataa
      481 agacattata caatataaaa atgaagaatt tattgaaaat aaactgccag agagaaaaag
      541 tatgcaacac tcccgccgag agtgtttgaa atggtgtacg gtacattttc gtgctaggag
      601 ttagatgtgc aggcagcaac gagaggggga gagatttttt tgggccttgt gaaattaacg
      661 tgagttttct ggtcatctga ctaatcatgt tggttttttg ttggtttatt ttgtttttat
      721 ctttgttttt atccagatta ggaaatttaa attttatgaa tttataatga ggtcaaacat
      781 tcagtcccag cgtttttcct gttctcactg tttagtcgaa tttttatttt aggctttcaa
      841 caaatgttct aactgtctta tttgtgacct cactttttat atttttttaa tttttaaaaa
      901 tattagaagt ttctaggata attttttcga cttttattct ctctaccgtc cgcactcttc
      961 ttacttttaa attaaattgt ttttttttca gttgggaaac actttgctca caagtttgta
     1021 caaaaaagca ggcttaatgg acaagaagta ctccatcgga ctcgacatcg gaaccaactc
     1081 cgtcggatgg gccgtcatca ccgacgagta caaggtccca tccaagaagt tcaaggtcct
     1141 cggaaacacc gaccgtcact ccatcaagaa gaacctcatc ggagccctcc tcttcgactc
     1201 cggagagacc gccgaggcca cccgtctcaa gcgtaccgcc cgtcgtcgtt acacccgtcg
     1261 taagaaccgt atctgctacc tccaggagat cttctccaac gagatggcca aggtcgacga
     1321 ctccttcttc caccgtctcg aggagtcctt cctcgtcgag gaggacaaga agcacgagcg
     1381 tcacccaatc ttcggaaaca tcgtcgacga ggtcgcctac cacgagaagt acccaaccat
     1441 ctaccacctc cgtaagaagc tcgtcgactc caccgacaag gccgacctcc gtctcatcta
     1501 cctcgccctc gcccacatga tcaagttccg tggacacttc ctcatcgagg gagacctcaa
     1561 cccagacaac tccgacgtcg acaagctctt catccagctc gtccagacct acaaccagct
     1621 cttcgaggag aacccaatca acgcctccgg agtcgacgcc aaggccatcc tctccgcccg
     1681 tctctccaag tcccgtcgtc tcgagaacct catcgcccag ctcccaggag agaagaagaa
     1741 cggactcttc ggaaacctca tcgccctctc cctcggactc accccaaact tcaagtccaa
     1801 cttcgacctc gccgaggacg ccaagctcca gctctccaag gacacctacg acgacgacct
     1861 cgacaacctc ctcgcccaga tcggagacca gtacgccgac ctcttcctcg ccgccaagaa
     1921 cctctccgac gccatcctcc tctccgacat cctccgtgta aggagttgaa cggctgaaaa
     1981 atcgatattt tgagcgaaaa aagccggaaa aatggatttt atcggataaa atttgatttt
     2041 ttgagctgag aaatgccttt atagacgaat ttccttgtca aattgttaaa aactgcaaaa
     2101 tttgccaaaa aaaaagcatt ttttatattt ttttttacaa aaaattccac aaaaatggca
     2161 aaatttgact aaaaatgcca attttgtcgt tttctccgtg ccacagcggc cgaaaatcga
     2221 tttttaagcg attttcgggt gaaaaagtgt aaaaaccgac tgaaaatcca gctgaaaacg
     2281 acaaaaacgg taagttttag ctaattttca cctttttttc gatatttttt tactaaaaaa
     2341 aaaccgaaaa attgagtttt tttcaaattt ctgcaaaaat tctgcaattt tagcaatttc
     2401 catactattt tgacgccgaa aaagcttaaa aatatgattt ttagaggttt ttaatggaaa
     2461 attcatggat ttttcaaatt tttccacgaa aatcattgaa aaatttgaaa aaattgattt
     2521 cctattcaaa gttctagcta aaaactgcaa ttttagcaat ttccatacta ttttagtact
     2581 gaaaaagcct aaaaatgtga ttttcagagg atttcaatgg aaaattcatg gatttttcca
     2641 atttttcaat gaaaaccatt gaaaaaccct ggaaaattga gtttttcttc aaagtttagg
     2701 aaatttatga gaatttttga ataaaaaatt tttaaatttg aaaaaaattg aaattttaat
     2761 tctaaatttt aaagaaagtt gattttttaa taattttttt ttttgaataa aaaaaaatca
     2821 actttcttta aaatttagaa ttaaaatttc aattttttta ttcaaaaatt ctcataaatt
     2881 tcgtaaactt ttaataaaaa ctcaattttt ccgatttttt tcgatgattt tcactaaaaa
     2941 attggaaaaa ttcatcaatt tcctattgaa aacctctaaa aattatattt tttttgcttt
     3001 tccgacgtca aaatagtatg gaaaatgcta aaattgcaga atttttccag aaatttgaag
     3061 aaaaaactca attttttccg ttttttcaat gattttcatg aaaaaattgg aaaattttgt
     3121 aaattttcca ttgaaaacct ctgaaaattt ggaaattttc gaatttaaat gtttaaaaaa
     3181 aattgaatat tttgcgagtt tttgaatttt ttttgagaat ttttgaattt ttttcaccca
     3241 aaattttttc aatttttcag gtcaacaccg agatcaccaa ggccccactc tccgcctcca
     3301 tgatcaagcg ttacgacgag caccaccagg acctcaccct cctcaaggcc ctcgtccgtc
     3361 agcagctccc agagaagtac aaggagatct tcttcgacca gtccaagaac ggatacgccg
     3421 gatacatcga cggaggagcc tcccaggagg agttctacaa gttcatcaag ccaatcctcg
     3481 agaagatgga cggaaccgag gagctcctcg tcaagctcaa ccgtgaggac ctcctccgta
     3541 agcagcgtac cttcgacaac ggatccatcc cacaccagat ccacctcgga gagctccacg
     3601 ccatcctccg tcgtcaggag gacttctacc cattcctcaa ggacaaccgt gagaagatcg
     3661 agaagatcct caccttccgt atcccatact acgtcggacc actcgcccgt ggaaactccc
     3721 gtttcgcctg gatgacccgt aagtccgagg agaccatcac cccatggaac ttcgaggagg
     3781 tcgtcgacaa gggagcctcc gcccagtcct tcatcgagcg tatgaccaac ttcgacaaga
     3841 acctcccaaa cgagaaggtc ctcccaaagc actccctcct ctacgagtac ttcaccgtct
     3901 acaacgagct caccaaggtc aagtacgtca ccgagggaat gcgtaagcca gccttcctct
     3961 ccggagagca gaagaaggcc atcgtcgacc tcctcttcaa gaccaaccgt aaggtcaccg
     4021 tcaagcagct caaggaggac tacttcaaga agatcgagtg cttcgactcc gtcgagatct
     4081 ccggagtcga ggaccgtttc aacgcctccc tcggaaccta ccacgacctc ctcaagatca
     4141 tcaaggacaa ggacttcctc gacaacgagg agaacgagga catcctcgag gacatcgtcc
     4201 tcaccctcac cctcttcgag gaccgtgaga tgatcgaggt gctgaagctc gaaaatttgg
     4261 agcttttaca cgagattttt gaagaaaaag cctgaaaatc gagagatttt gagctgtgga
     4321 attcgttgaa attacataat tcgcgcccgt aattctttaa aaaaacgctg aaatttgcag
     4381 attttgccag aaagttgaag aaaaatctac ttttccggca ttctctatta ttttcatgga
     4441 aaattagaaa aatccatgaa taaacagtga aaattgctga ttttacctga aattttgaaa
     4501 aaaaaaaaaa tctaaaatca aggttttaag cctaaaaact gcagatttca gccaaaattg
     4561 tgaaaaaagc tgattttttg ctattttgag cttttccatg gggtggaaat tgccaaaaat
     4621 tacggaacat tcacacaaat ttgctgaata atccaatttt ccagtgtttt ctagagtttc
     4681 cgtagaaatt ttgaaaaaac ccagtaaatt ttcaatcgga aaacctctaa aaacatcgtt
     4741 tcaggcctat aaatagtaaa aattaccgat tttattcgaa aatttaagga aaagtcgtga
     4801 aaaacgagat tttaagcctc aaaactgcag attctatcta aaattaaaaa aaaaaaacat
     4861 aaatatccga atttttggag gaaaagcctg aaaatcgata ttttaagcct caaaactgaa
     4921 gatttcagcc aaaaaaaaaa acagattttt cgctatttta tgtgccaaaa aaccacattc
     4981 cgagcttttc cagagtggaa attgccaaaa attacagaat tttcccagaa attagcaaaa
     5041 aaaaaaccca tttttcaggc gttttcaacg attttcatag caaatttgaa aaatccatga
     5101 attttttaat tatagaaaac cctctgaaaa ttgcagtttt aagctattcc agcattcaaa
     5161 tagtatggaa attgctaaaa attgaagaat tttcccaaaa atttgatgaa aacttatttt
     5221 tctggcgttt tcgatgattt tcatggaatt tcgagaaaat taatgaattt tcaattaaaa
     5281 aacgtctgca aaatcacatt ttttaaagct ttttcagcaa caaaatagta tggaaattgc
     5341 tttttaaaaa agcggaattt ttggaaaaaa ggttaaattt tcagtcagtt tttgcacttt
     5401 ttgactgaaa attcactgaa attagggaaa aattccggaa aaatgatttt ttcgaattgt
     5461 agggcaaatt ttgcaatttc aagcaatttc cataatattt taatgctgga aaagctcaaa
     5521 tttacgtttt aatgggattt tagtgaaaaa ttcatggatt ttccgaaaaa tttgcaggaa
     5581 aatcatagta aagacctcaa aaaatcaata aaaaatttaa aaaattggac aatcacattt
     5641 ctgcgtaaaa tctgcacttt ttcagtaaaa aataccgatt ttccgtgaaa attctcgatt
     5701 tttaatcgaa aatctcgaaa tttttacaaa tttcagcacc aaaatcgttg aaaattctga
     5761 aaatttgaat tttttttcct tgttttttga gtaaaatttg cactttttca gtaaagaata
     5821 ccgattttcc gtgaaaattc tcggttttta atcgaaaatc tcgaaatttt tacaaatttc
     5881 agctccgaaa tagctgaaaa ttggacaaaa atttcaattt ttgccctgtt ttttgagtaa
     5941 aatctgcact ttttagttga aaaaaaacac ggattttgta ggaaaatttg ggaaaaatct
     6001 ccaaattttc acaattttcc agtaaaaaca gcaattttta aaacggtttt taatcaaaaa
     6061 tctttagaat tttccaaatt tcagcaccaa aaaagctaaa aatcgctgaa attttcgaca
     6121 aattccaaat ttccaggagc gtctcaagac ctacgcccac ctcttcgacg acaaggtcat
     6181 gaagcagctc aagcgtcgtc gttacaccgg atggggacgt ctctcccgta agctcatcaa
     6241 cggaatccgt gacaagcagt ccggaaagac catcctcgac ttcctcaagt ccgacggatt
     6301 cgccaaccgt aacgtaagtt taaacatgat tttactaact aactaatctg atttaaattt
     6361 tcagttcatg cagctcatcc acgacgactc cctcaccttc aaggaggaca tccagaaggc
     6421 ccaggtctcc ggacagggag actccctcca cgagcacatc gccaacctcg ccggatcccc
     6481 agccatcaag aagggaatcc tccagaccgt caaggtcgtc gacgagctcg tcaaggtcat
     6541 gggacgtcac aagccagaga acatcgtcat cgagatggcc cgtgagaacc agaccaccca
     6601 gaagggacag aagaactccc gtgagcgtat gaagcgtatc gaggagggaa tcaaggagct
     6661 cggatcccag atcctcaagg agcacccagt cgagaacacc cagctccaga acgagaagct
     6721 ctacctctac tacctccaga acggacgtga catgtacgtc gaccaggagc tcgacatcaa
     6781 ccgtctctcc gactacgacg tcgaccacat cgtcccacag tccttcctca aggacgactc
     6841 catcgacaac aaggtcctca cccgttccga caagaaccgt ggaaagtccg acaacgtccc
     6901 atccgaggag gtcgtcaaga agatgaagaa ctactggcgt cagctcctca acgccaagct
     6961 catcacccag cgtaagttcg acaacctcac caaggccgag cgtggaggac tctccgagct
     7021 cgacaaggcc ggattcatca agcgtcagct cgtcgagacc cgtcagatca ccaagcacgt
     7081 cgcccagatc ctcgactccc gtatgaacac caagtacgac gagaacgaca agctcatccg
     7141 tggttggttt ttccgagaga aaaacgctga aaaatgccga aaattttgaa tttgcaggct
     7201 ttttaggtgc aaaagtacgg aaaaatcgga aaaaaatcag gaaaacatgg ggaaaatcca
     7261 attttccaga aaaaatgttt gaaaaaattg aaaacttttg tttttttagt aataaagttt
     7321 taaaaaatgg caaaaatagc tgaaaacagc gaaaaaaaaa aatgtttttt tcaggaaaaa
     7381 atttgcaaaa aaatgaagag caacaataat tccctttttt ttctgtgttt ttttcggaaa
     7441 aattgagatc gaaaacgaaa aacagcgaaa attcccaatt ttcccagttt ttaaaacatt
     7501 tttttgtaaa aatttggaaa aaacgagttt tttcagaaaa aaatgtgaaa aaaaaggtaa
     7561 attaccaatt tatgcttttt tttcgatcga gtaaaaaatc gatttttcca agatttttcg
     7621 cgaaaaattc aaaaaaaaag aacgaaaatt ctcgattttc tacttttttt cgaaaaaaaa
     7681 aggaaattat agatttttcc aagatttttc gcgaaaaaat tcgaaaaaaa tgtttttttc
     7741 gaaaatttca gattttctaa ttttttcaga agaattcgaa aaaaaaaatc aaaaaattcg
     7801 caatttttag atttttttaa aatctcaatt ttctataatt attttcctaa aaaaatgtaa
     7861 ttaaaaaacc gctaaaaacg gtaaattcca aatttttgca ttttttcaaa aaaaatttga
     7921 aaaaaaaaat cagaaaattc tcaaatctat tccctttttt taaaattttt cccaaaccaa
     7981 aaaaaaaaac ggtaaatttt caaatttagc atttttccaa aattaaacat aaaaaaaaac
     8041 gcaaatttcc aaatttcgtt tttttttcaa aaaagttgaa aaatccagaa aataccctct
     8101 tgtccatgaa aaaatacgaa aattttcaat aaaaaatcaa tttcttctta aaaaattaat
     8161 tctcgatttt tccatgaaaa aaaatcaaat attaattaaa tttgaaaaaa aaaagaattt
     8221 atctaatagt ttcgtttttt ttcagaggaa aaatttgaaa aaaaaaacag taaattctct
     8281 ttctttttga aaaaaaaaat tatcgattct tccaagtttt ttcccgaaaa aatcgacaaa
     8341 aaaacgagaa tttccgattt tctatttttt ttttcaaaaa aatgtgaaaa aaaaaattaa
     8401 aaaattccaa ttttgcattt ttttttcaaa aaaaatttaa aaacaccgta taattcccga
     8461 tttttccatg aaaaaatacg aaaaattcca ataaaaatca actattttaa aaatttgccg
     8521 ttttttcaga ggtcaaggtc atcaccctca agtccaagct cgtctccgac ttccgtaagg
     8581 acttccagtt ctacaaggtc cgtgagatca acaactacca ccacgcccac gacgcctacc
     8641 tcaacgccgt cgtcggaacc gccctcatca agaagtaccc aaagctcgag tccgagttcg
     8701 tctacggaga ctacaaggtc tacgacgtcc gtaagatgat cgccaagtcc gagcaggaga
     8761 tcggaaaggc caccgccaag tacttcttct actccaacat catgaacttc ttcaagaccg
     8821 agatcaccct cgccaacgga gagatccgta agcgtccact catcgagacc aacggagaga
     8881 ccggagagat cgtctgggac aagggacgtg acttcgccac cgtccgtaag gtcctctcca
     8941 tgccacaggt caacatcgtc aagaagaccg aggtccagac cggaggattc tccaaggagt
     9001 ccatcctccc aaagcgtaac tccgacaagc tcatcgcccg taagaaggac tgggacccaa
     9061 agaagtacgg aggattcgac tccccaaccg tcgcctactc cgtcctcgtc gtcgccaagg
     9121 tcgagaaggg aaagtccaag aagctcaagt ccgtcaagga gctcctcgga atcaccatca
     9181 tggagcgttc ctccttcgag aagaacccaa tcgacttcct cgaggccaag ggatacaagg
     9241 aggtcaagaa ggtgcttaca attttctgga gaaaaatatt ttaaaaaata acgaaaatgg
     9301 accgaatttt tggagaaaat tttaaaaaaa acgacatttt tcccaaaaaa ttgaaaaatt
     9361 tccgaaaaaa cgttcaaaat tttcaaaaaa ggagcatttt tcgaaaaaaa aaacgataat
     9421 acggaaattt tttgattttc cagttttttt ctcaaagaaa atcggaaaaa ggtttttttt
     9481 ctttaaaaac gtccaaaatt tttcgaaaaa agagaaaatt ttcgattttc cgattttttt
     9541 cagaaaaaaa ttgaaaaaaa aagaaatttt ccgattttct cagagaaaga cgaaaagacg
     9601 atatttttcg gaaaaaagtt gaaaaatcaa aaaaaaaacc gaaaaacggc cgaaattttc
     9661 taaaaattgg aaatattgtc gatttttcga aaaaaaagag tcaaaagttt tcaaaaaaat
     9721 atattttttc gattttccgt tttttttttt cagtaaacat tgaaacaaac gaaatttttc
     9781 caaaaaaatc caattatttt cccaaatatc gaaatatttt tgattttccg tttttttcag
     9841 aaaaaagtta aaaaaaaaag ggaaaattaa gattttcctg attttttttg agaaaaaatc
     9901 gataaaaatt gaatattctg gaaaaaacct ttaaaaaatt cgaattaaat cgaaaaacgt
     9961 ccaaaatttt ctaaaaaatt gaaatttttt ttgaaaaaaa acggaaaaat ttagatttcc
    10021 cgactttttt caatgaaaaa tcgaattttc gttgactttt cattaaaaaa tccaaacgca
    10081 tttttttcca ctaaaatctc gaaaaagtgt catattgcac catattgttt ccacattcat
    10141 attattttga gctaaatcag gtgattttct gtattttgat actaatttct atttttagaa
    10201 aaaatgtggg aacaatatgg tgcaatatga cattttttcg agattttagt ggagaaaaaa
    10261 tgcgtttgga aacgaatttt ataataaaaa gtcaacgaaa attcgatttt tcatggaaaa
    10321 atagggaaat cgaaaacaaa tttgcttttt tttcaaaaaa aaaaacggaa attcgaaatt
    10381 tttccgttct ttcaaaaaaa aaatcgattt ttttggaaat tttttttttc aaatttttaa
    10441 aaatttccag gacctcatca tcaagctccc aaagtactcc ctcttcgagc tcgagaacgg
    10501 acgtaagcgt atgctcgcct ccgccggaga gctccagaag ggaaacgagc tcgccctccc
    10561 atccaagtac gtcaacttcc tctacctcgc ctcccactac gagaagctca agggatcccc
    10621 agaggacaac gagcagaagc agctcttcgt cgagcagcac aagcactacc tcgacgagat
    10681 catcgagcag atctccgagt tctccaagcg tgtcatcctc gccgacgcca acctcgacaa
    10741 ggtcctctcc gcctacaaca agcaccgtga caagccaatc cgtgagcagg ccgagaacat
    10801 catccacctc ttcaccctca ccaacctcgg agccccagcc gccttcaagt acttcgacac
    10861 caccatcgac cgtaagcgtt acacctccac caaggaggtc ctcgacgcca ccctcatcca
    10921 ccagtccatc accggactct acgagacccg tatcgacctc tcccagctcg gaggagactc
    10981 tggaggatct ggaggaactg gaggatcttc cgccggcggg agctctatga gccgtagacg
    11041 aaaagcgaat ccgacaaaac tgagtgaaaa cgcgaagaag cttgccaagg aagttgaaaa
    11101 ttaagataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
    11161 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
    11221 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
    11281 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
    11341 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
    11401 aggaaacttt attgtgccgc caagaaaaaa gtctcaaagc ttggcgtaat catggtcata
    11461 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11521 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11581 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    11641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    11701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11761 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11821 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11881 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11941 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12001 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12061 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12121 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12181 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12241 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12301 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12361 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12421 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12481 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12541 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12721 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13561 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    13621 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
