LOCUS       pMLS1272                3707 bp DNA     circular SYN 07-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             complement(460..528)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     599..616
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     primer_bind     625..645
                     /locus_tag="T7"
                     /label=T7
     misc_feature    651..715
                     /locus_tag="MCS-inverted in SK+"
                     /label=MCS-inverted in SK+
     misc_feature    965..1314
                     /locus_tag="U6 promoter"
                     /label=U6 promoter
     misc_feature    1313..1314
                     /locus_tag="Sap Trap gg Overlap"
                     /label=Sap Trap gg Overlap
     misc_RNA        1315..1335
                     /locus_tag="SNAP-25 gRNA"
                     /label=SNAP-25 gRNA
     misc_feature    1336..1418
                     /locus_tag="sgRNA scaffold"
                     /label=sgRNA scaffold
     misc_feature    complement(1336..1338)
                     /locus_tag="Sap Trap gg Overlap(1)"
                     /label=Sap Trap gg Overlap
                     /label=Sap Trap gg Overlap(1)
     misc_feature    1460..1506
                     /locus_tag="MCS-inverted in SK+(1)"
                     /label=MCS-inverted in SK+
                     /label=MCS-inverted in SK+(1)
     primer_bind     complement(1515..1534)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(1552..1572)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(1578..1600)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(1942..2624)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(2722..3381)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatgtggt
      721 gacatgtgta actgcatttt ttagagacaa gttagggtga aaaaacgtta cttttgtatt
      781 gggtgtttaa aaattgaaaa gcgcttttaa tccatagcca gatttggttt ttttaataag
      841 tgctgaaaat gtgtgtgcct ttaaaaagaa cagtagtcgt aattatgttt tcttgctttt
      901 ctaaattttg caggttaatc aatttttttc acatgaaata aaatatattt tcccaaaaat
      961 ttaacaaaaa aaactagcaa taaaggaata aaaaactgta caccttaaag gcgcacactc
     1021 tgttttgcaa attttatttt tagttgtgaa ttttctgctg agacctgaaa atagcaactt
     1081 tagtactact ataatttgtc aaccttttca aaaaaagcat gcaatttttg agaaactctt
     1141 ataaaagcta ttattaaaaa aacacctttt ttccaaaatt attccacaaa aaatatgtta
     1201 tgaaatgcct acaccctctc acacacactc tttatactac tctgtcaaac tcacgagatg
     1261 tctgccgcct cttgtgtccc ccctatataa acacctccta ttgcgagatg tcttgagagg
     1321 ctggaatcaa aacttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     1381 tcaacttgaa aaagtggcac cgagtcggtg cttttttttg tgaaattttc tataattttt
     1441 tttgcagaca tgtgtggtga tcaagcttat cgataccgtc gacctcgagg gggggcccgg
     1501 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     1561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcata tgcgcttcct cgctcactga
     1801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2401 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
