LOCUS       pMLS621                 9275 bp DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..145
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             782..1441
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1539..2221
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_recomb     2355..3339
                     /locus_tag="ttTi5605 left recomb"
                     /label=ttTi5605 left recomb
     misc_feature    3222..3241
                     /locus_tag="oCF176"
                     /label=oCF176
     misc_feature    3691..3693
                     /locus_tag="AfeI"
                     /label=AfeI
     misc_feature    3694..3699
                     /locus_tag="GFP w/ syntrons(1)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(1)
     misc_feature    3694..3699
                     /locus_tag="syntron3(3)"
                     /label=syntron3
                     /label=syntron3(3)
     misc_feature    3700..3719
                     /locus_tag="GFP w/ syntrons"
                     /label=GFP w/ syntrons
     misc_feature    3700..3719
                     /locus_tag="syntron3"
                     /label=syntron3
     misc_feature    3720..3753
                     /locus_tag="Lox2272"
                     /label=Lox2272
     gene            complement(3754..5871)
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label=C.Briggsae unc-119(+)
     CDS             complement(4038..4883)
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
     misc_feature    complement(4145..4195)
                     /locus_tag="Intron"
                     /label=Intron
     misc_feature    complement(4573..4615)
                     /locus_tag="Intron(1)"
                     /label=Intron
                     /label=Intron(1)
     misc_feature    complement(4732..4826)
                     /locus_tag="Intron(2)"
                     /label=Intron
                     /label=Intron(2)
     misc_feature    5872..5905
                     /locus_tag="Lox2272(1)"
                     /label=Lox2272
                     /label=Lox2272(1)
     misc_feature    5906..5924
                     /locus_tag="GFP w/ syntrons(2)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(2)
     misc_feature    5906..5924
                     /locus_tag="syntron3(1)"
                     /label=syntron3
                     /label=syntron3(1)
     misc_feature    5925..5930
                     /locus_tag="GFP w/ syntrons(3)"
                     /label=GFP w/ syntrons
                     /label=GFP w/ syntrons(3)
     misc_feature    5925..5930
                     /locus_tag="syntron3(2)"
                     /label=syntron3
                     /label=syntron3(2)
     misc_feature    5931..5933
                     /locus_tag="AfeI(1)"
                     /label=AfeI
                     /label=AfeI(1)
     misc_feature    5944..5951
                     /locus_tag="PmeI"
                     /label=PmeI
     primer_bind     6024..6044
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_recomb     6060..6184
                     /locus_tag="attR4 recomb"
                     /label=attR4 recomb
     CDS             6224..6529
                     /locus_tag="ccdB"
                     /label=ccdB
     CDS             complement(6874..7530)
                     /locus_tag="CmR"
                     /label=CmR
     misc_recomb     complement(7639..7763)
                     /locus_tag="attR3"
                     /label=attR3
     primer_bind     complement(7771..7788)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     7848..9275
                     /locus_tag="ttTi5605 right recomb"
                     /label=ttTi5605 right recomb
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt agcgtaagtt taaacatgat tttactaaca
     3721 taacttcgta taaagtatcc tatacgaagt tatgacattc tctaatgaaa aaatctttca
     3781 gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt ccgtgtgatt
     3841 agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg gggaactgaa
     3901 aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact agacaggaat
     3961 tattggaatt taatcacatt tggagttttt tttctattcg acagaataat tatccagaac
     4021 atttttgtat taaatattta tgcatcatat gagtagtcgg ctttgttgtg catgacgagt
     4081 ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt ttggattgtt gatcatgtcg
     4141 tccactgaaa aagagattag tcttttgaat tgtacttttt agaataatga ctcactgagt
     4201 tgttgagaga gttgagggaa ctcatagata tgttcacagt tgtttcgtga attcggaata
     4261 cagaatccga attcaaagtc aaaacacttc agaaggcgat ctttgaagaa gtgacgttcg
     4321 atcattcgga aatgatggat tgggatgtct cctactttga attccacagt tgctccgacc
     4381 gttttgagtt tcaaaaagtt tggagcaaat ctataacgca cgtatcttgc cgactcctgt
     4441 ggcgactcat cattctcctg atcgttctcc ggtttggcga tctcgaaaag cacttgctca
     4501 gtgtccagat cacggatttg gaacttggtg aactcgatgt tatagatgtt cgcagatggg
     4561 gagcataaga atcctaaatt tatgttttaa actgaaatcc aaagggagca agataccttg
     4621 agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt tgagctttct tcgcaagctc
     4681 cgattccgtt gtgattcctt gttcggtgct tggtggtggc cgtggcatct ggaaatatgg
     4741 aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag ttagttgagc
     4801 ggtttctcta gttttctgag tctcacctgc gacgggaagg tcgccgagcc gggtggaatc
     4861 gattgttgtt gctcggcttt catatcggtt tggttggaag cggctgaaaa cggaaagaag
     4921 tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt gccaaataac
     4981 cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga taatgatatc
     5041 agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac atgtaatcaa
     5101 aatacacttt gtaaattatc atttttattg aaactccacc atttttctat ttataacgct
     5161 aataatttga aaaagaaacc tgttgcgaac cgcggggtga atcccaaaaa cgaatgcgtt
     5221 ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga atagagagct
     5281 cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg agggtcttct
     5341 tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg agaagggaaa
     5401 aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag cggaaaagca
     5461 gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc caagaaagga
     5521 atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca gaataacaga
     5581 tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta atttgggtca
     5641 atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg tttgttatca
     5701 gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt ttctgtcaaa
     5761 tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac attttatccg
     5821 ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa gataacttcg
     5881 tataaagtat cctatacgaa gttattaact aatctgattt aaattttcag gctcacgcac
     5941 gtggtttaaa ctagaactag gcccgggcta cgtaatacga ctcacttaag gccttgacta
     6001 gagggtacca gagctcacct aggcaggaaa cagctatgac catgattacg ccaagctatc
     6061 aactttgtat agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     6121 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     6181 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     6241 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     6301 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     6361 cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg
     6421 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     6481 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     6541 ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     6601 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     6661 gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     6721 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     6781 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     6841 tctggctttt agtaagccgg atcctctaga ttacgccccg ccctgccact catcgcagta
     6901 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     6961 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     7021 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     7081 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     7141 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     7201 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     7261 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     7321 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     7381 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     7441 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     7501 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     7561 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
     7621 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
     7681 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     7741 ttcaacttta ttatacatag ttgataattc actggccgtc gttttacact cgagacgtac
     7801 ggtgcgcgcg atgcattcga agatctgccc actagtgagt cgtattataa gtgcaagtaa
     7861 gatcagtgtt tgtttcgata tcagtctgtt tcgtaacggt cttctgtata actacaaaaa
     7921 agaataaaaa accgtatcac aactgagaaa aaagacaaaa cttattgaac agtgttcctt
     7981 tcaaaaaatt attttgtaaa aaatcgagtt tttctccaaa ataacgcacg caatcaaaaa
     8041 caagaaaact aacgtgttct cctgggcgtg gaactcctta tcaagattac cattattgtc
     8101 tgtcagaatt gatatttcaa ttatttcata tctagacaaa cccgggtgtc ttgcatttat
     8161 tagtactgtg agtttacata caaacgaggc ttactcagat aacatagagt cacctctaat
     8221 tagcaaaaac ccatttctaa ttagctattt cgaccagttc taaagtcacc gtaattagaa
     8281 atggtttttg tagcttttct aattagaaat gaccccgaga taagtgaaat atggcatttt
     8341 ctgggttttc tagtaagaaa tgatcccgca cgtagtgatt ccgcgttaca agccaaattc
     8401 aaattaaaaa agtatattac acaattcaag ttacattatt ttcaggaaac aatggcgaag
     8461 agcaaagcag agattcaaaa ggaggaaaga gacaacagat atgaacgaag acttcttgaa
     8521 aagagaaaga gacatgaggt gagtaaccat gataataaat aatcaagtaa gggctataga
     8581 tttataaatt ctggagttaa cagtttcgaa aaaaagtagt tttcatatgt gcgaaggaat
     8641 atcaaaaata ggggaatagc ctaaacgagt gagaaaatta aatgtttgtt attttttcaa
     8701 aaatgtctgc agggccgtta tcctttcaaa tgcactctat caaacataga tcatatccat
     8761 catatcttat ccagaattta tatcatatcc aagattgaga gcgtttcaag gaaaatctca
     8821 aacaaccata caattttgta gatcgacaac aaggcctcca cttcttcatc gatggcctct
     8881 gcagcacctg caccaccaag tttgtatttt tcatattaat ataaagtgag ccacgcatag
     8941 tcgtttttag gatttttgcc agatcgatgt cgcgcaaaca gactgttttt ttttctttga
     9001 aagccgtgcg tgacatgggc cacggattgt aacgccgcgc tggatggata aaaatttatt
     9061 cagtttttta gcctttacaa aatatggttc gactactttc tttattttta catttttata
     9121 ggtgctcatt gagctgagat attttgggta taatcggaaa atcgctgatt ttggtcattt
     9181 ttctgcacta aagattacac aaaaagttca gtatttagat tcattgttga aaattgggga
     9241 tgatctacag atacatatcg ccgtgttcaa aaaca
//
