LOCUS       pBBR1_DVK_GH            3211 bp ds-DNA     circular     13-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     LacZ            complement(5..500)
                     /label="LacZα cassette"
     Origin          642..1413
                     /label="pBBR1 oriV"
     misc_feature    1414..2076
                     /label="pBBR1 Rep"
     Origin          2087..2195
                     /label="partial oriT"
     misc_feature    complement(2216..3182)
                     /label="KanR"
ORIGIN
        1 TGCCagagac ctgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
       61 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      121 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      181 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
      241 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt
      301 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      361 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      421 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      481 gccaacgcgc gggggtctct ACTAatgtct tcagcagtag gacaaatccg ccgccctaga
      541 cagctgggcg cgccccccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc
      601 gctcccttgc cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg
      661 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      721 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      781 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      841 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      901 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      961 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
     1021 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
     1081 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
     1141 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
     1201 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
     1261 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
     1321 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
     1381 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
     1441 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
     1501 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1561 cagatgggcc accagaacgc cgtggtggtC AGCCAGAAAA CACTTTCCAa gctcatcgga
     1621 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1681 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1741 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1801 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1861 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1921 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
     1981 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     2041 gagccgccga cacgggtaac gctgccgcgc cggtagggcc ggcctacggc cagcctcgca
     2101 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     2161 cgctcgcggg tgggcctact tcacctatcc tgcccTTGTG TCTCAAAATC TCTGAtgtac
     2221 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2281 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta
     2341 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2401 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2461 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2521 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2581 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
     2641 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2701 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2761 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2821 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     2881 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2941 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3001 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3061 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta aggtaactgt
     3121 cagaccaagt ttactcatat atactttaga ttgatttttc gttCCACTGA GCGTCAGACC
     3181 CCAGAACTCT GTCAGACCga gtggaagaca t
//