LOCUS       pREDawn-MCS             8929 bp ds-DNA     circular     28-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(26..73)
                     /label="T7 terminator_regulatory"
                     /note="label: T7 terminator_regulatory"
     misc_feature    complement(140..157)
                     /label="6xHis"
                     /note="label: 6xHis"
     misc_feature    179..236
                     /label="MCS"
     CDS             complement(205..236)
                     /label="T7 tag (incomplete) - G missing at position 208"
                     /note="codon_start: 1 note: promotes efficient translation in E. coli product: leader peptide from bacteriophage T7 gene 10 transl_table: 1 translation: MASMTGGQQMG"
     misc_feature    complement(240..257)
                     /label="thrombin site"
     misc_feature    complement(267..284)
                     /label="6xHis"
                     /note="label: 6xHis"
     RBS             303..325
                     /label="efficient ribosome binding site from bacterioph"
                     /note="label: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      401..428
                     /label="T7Te terminator"
                     /note="label: T7Te terminator"
     terminator      444..515
                     /label="rrnB T1 terminator"
                     /note="label: rrnB T1 terminator"
     misc_feature    complement(563..595)
                     /label="ssrA tag (LVA)"
     CDS             complement(596..1306)
                     /label="-lambda repressor"
     RBS             1313..1324
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /note="label: strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
     misc_feature    complement(1331..1577)
                     /label="FixK2-promoter regulatory"
                     /note="label: FixK2-promoter regulatory"
     misc_feature    complement(1647..1666)
                     /label="pBRrevBam"
     promoter        1872..1949
                     /label="lacIq promoter"
                     /note="label: lacIq promoter"
     misc_feature    1953..2555
                     /label="DrHO"
                     /note="label: DrHo1"
     misc_feature    2555..4072
                     /label="DrBphP (1-506)"
                     /note="label: DrBphP (2-506)"
     misc_feature    4073..4120
                     /label="Linker"
     misc_feature    4073..4807
                     /label="BjFixL-HK"
                     /note="label: BjFixL-HK"
     misc_feature    4807..5421
                     /label="FixJ"
                     /note="label: FixJ"
     CDS             6225..6416
                     /label="rop"
                     /note="label: rop"
     misc_feature    6518..6660
                     /label="Bom"
     rep_origin      complement(6828..7510)
                     /label="ColE1 origin_rep_origin"
                     /note="direction: LEFT label: ColE1 origin_rep_origin"
     CDS             7556..8371
                     /label="KanR"
                     /note="label: KanR resistance marker_CDS"
     rep_origin      complement(8463..8918)
                     /label="M13 origin_rep_origin"
                     /note="direction: LEFT label: M13 origin_rep_origin"
     rep_origin      complement(8595..8901)
                     /label="F1 origin_rep_origin"
                     /note="direction: LEFT label: F1 origin_rep_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcAAGCTTgt
      181 cgacggagct cgaattcgga tccgaccatt tgctgtccac cagtcatgct agccatATGg
      241 ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat gatggctgct gcccatggta
      301 tatctccttc ttaaagttaa acaaaattat ttctagagca accattatca ccgccagagg
      361 taaaatagtc aacacgcacg gtgttactct agtatataaa cgcagaaagg cccacccgaa
      421 ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag
      481 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggctctagt agcgatctac
      541 actagcacta tcagcgttat taagctacta aagcgtagtt ttcgtcgttt gcagcgccaa
      601 acgtctcttc aggccactga ctagcgataa ctttccccac aacggaacaa ctctcattgc
      661 atgggatcat tgggtactgt gggtttagtg gttgtaaaaa cacctgaccg ctatccctga
      721 tcagtttctt gaaggtaaac tcatcacccc caagtctggc tatgcagaaa tcacctggct
      781 caacagcctg ctcagggtca acgagaatta acattccgtc aggaaagctc ggcttggagc
      841 ctgttggtgc ggtcatggaa ttaccttcaa cctcaagcca gaatgcagaa tcactggctt
      901 ttttggttgt gcttacccat ctctccgcat cacctttggt aaaggttcta agctcaggtg
      961 agaacatccc tgcctgaaca tgagaaaaaa cagggtactc atactcactt ctaagtgacg
     1021 gctgcatact aaccgcttca tacatctcgt agatttctct ggcgattgaa gggctaaatt
     1081 cttcaacgct aactttgaga atttttgcaa gcaatgcggc gttataagca tttaatgcat
     1141 tgatgccatt aaataaagca ccaacgcctg actgccccat ccccatcttg tctgcgacag
     1201 attcctggga taagccaagt tcatttttct ttttttcata aattgcttta aggcgacgtg
     1261 cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt gctcatctag tatttctcct
     1321 cttttctaga ctccgttgtg atgacgcatt ggtacgcggt atcgggaggt tcgaaaattt
     1381 cgagcgatat cttaaggggg gtgccttacg tagaaccccg taggtcatgc ccgaggccgg
     1441 tcctggatgg cgcggcggat acgcttgagc aggttttcgt cgagaagcgg cttcaaaacc
     1501 acgtctttta cgccggcctc ggcggcccgg gtcgagatgt tttcgtccgg atagccggtg
     1561 atcaggatca cgggcgtaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac
     1621 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     1681 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     1741 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     1801 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     1861 tcgagatccc ggacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc
     1921 cggaagagag tcaattgagg gtggtgaatg tgCTCCGACT CATGATCATG AAGCTTTTAC
     1981 AAAGGGAGAC CCTGGACCTG CACCGCGAGG TCGAGGCCCT GATGCCCGTG ATGAGTGAGA
     2041 CGCTGACCCG GCGGGGCTAT CTGGAGGTGC TGCGGCGCCT GCAAGCGGTG GTCGCGCCGC
     2101 TGGAAGCACA ACTCGACGCC CTGCCGCTGC CGGACGAGCT GGCGTGGCCC GAGCGCCGCA
     2161 AGGCCCATCT GCTCGCTCTG GACCTTGCCG TAATGGGGGA AGGGCCGTCA AATCAGCAGG
     2221 CGCCACTGTC TGTGCCCGAG CTGACCACTC TGGCGCAGGC CTTCGGAGCG CTTTACGTGC
     2281 TTGAAGGCGC GACGCTAGGC GGACAGCTCA TCAGCCGCCA TCTCAGGCGC ACGCTGGGGC
     2341 TAAGCCCTGA GCAGGGCAGC GCCTATTTCT CCGGCTATGG CCCCCAGACC GGCCCGCGCT
     2401 GGCGCTCGTT CGGGGAAGTG CTGGAAGCCA GCGTGCCGGC AGAAGACGCG GCAGAGGTCG
     2461 TGGCGGGAGC GCGGCAGACC TTCGGGGCCT TCCGCCGCGC CTTGCAGGGG CTGTCGGAAG
     2521 CTGAAGCGGT CCAAGTGCCT GAGGTCGCCC ACGCATGAgc cgggacccgt tgcccttttt
     2581 tccaccgctt taccttggtg gcccggaaat taccaccgag aactgcgagc gcgagccgat
     2641 tcatattccc ggcagcatcc agccgcacgg cgccctgctc actgccgacg ggcacagcgg
     2701 cgaggtgctc cagatgagcc tcaacgcggc cacttttctg ggacaggaac ccacagtgct
     2761 gcgcggacag accctcgccg cactgctgcc cgagcagtgg cccgcgctgc aagcggccct
     2821 gccccccggc tgccccgacg ccctgcaata ccgcgcaacg ctggactggc ctgccgccgg
     2881 gcacctttcg ctgacggtgc accgggtcgg cgagttgctg attctggaat tcgagccgac
     2941 ggaggcctgg gacagcaccg ggccgcacgc gctgcgcaac gcgatgttcg cgctcgaaag
     3001 tgcccccaac ctgcgggcgc tggccgaggt ggcgacccag acggtccgcg agctgacggg
     3061 ctttgaccgg gtgatgctct acaaatttgc ccccgacgcc accggcgaag tgattgccga
     3121 ggcccgccgt gaggggctgc acgcctttct gggccaccgt tttcccgcgt cggacattcc
     3181 ggcgcaggcc cgcgcgctct acacccggca cctgctgcgc ctgaccgccg acacccgcgc
     3241 cgccgccgtg ccgctcgatc ccgtcctcaa cccgcagacg aatgcgccca ccccgctggg
     3301 cggcgccgtg ctgcgcgcca cctcgcccat gcacatgcag tacctgcgga acatgggcgt
     3361 cgggtcgagc ctgtcggtgt cggtggtggt cggcggccag ctctggggcc tgatcgcctg
     3421 ccaccaccag acgccctacg tgttgccgcc cgacctgcga accacgctcg aatacctggg
     3481 ccgcttgctg agcctgcaag ttcaggtcaa ggaagcggcg gacgtggcgg cctttcgcca
     3541 gagcctgcgg gagcaccacg cgcgggtggc cctcgcggcg gcgcactcgc tctcgccgca
     3601 cgacaccctc agtgacccgg cgcttgacct gctgggcctg atgcgggccg ggggcctgat
     3661 tctgcgtttc gagggccgct ggcagacgtt gggtgaagtg ccgcctgccc cggcggtgga
     3721 cgcgctgctg gcgtggctcg aaacccagcc gggcgccctg gtccagaccg acgcgctAgg
     3781 ccaactgtgg cccgccggcg ccgatctcgc ccccagcgca gcgggcctgc tcgccatcag
     3841 cgtgggcgag ggctggtcgg agtgcctcgt ctggctgcgg cccgaactgc ggctggaggt
     3901 cgcctggggc ggggccactc ctgaccaggc gaaagacgac ctcgggccgc gccactcatt
     3961 cgacacctac ctcgaagaaa aacgcggcta cgccgagccc tggcatcccg gcgaaatcga
     4021 ggaggcgcag gatctacgtg acacattgac cggggcgctg ggcgagcgcc tgctccagga
     4081 actgcaatcc gagctcgtcc acgtctccag gctgagcgcc atgggcgaaa tggcgtccgc
     4141 gctcgcgcac gagctcaacc agccgctggc ggcgatcagc aactacatga agggctcgcg
     4201 gcggctgctt gccggcagca gtgatccgaa cacaccgaag gtcgaaagcg ccctggaccg
     4261 cgccgccgag caggcgctgc gcgccggcca gatcatccgg cgcctgcgcg acttcgttgc
     4321 ccgcggcgaa tcggagaagc gggtcgagag tctctccaag ctgatcgagg aggccggcgc
     4381 gctcgggctt gccggcgcgc gcgagcagaa cgtgcagctc cgcttcagtc tcgatccggg
     4441 cgccgatctc gttctcgccg accgggtgca gatccagcag gtcctggtca acctgttccg
     4501 caacgcgctg gaagcgatgg ctcagtcgca gcgacgcgag ctcgtcgtca ccaacacccc
     4561 cgccgccgac gacatgatcg aggtcgaagt gtccgacacc ggcagcggtt tccaggacga
     4621 cgtcattccg aacctgtttc agactttctt caccaccaag gacaccggca tgggcgtggg
     4681 actgtccatc agccgctcga tcatcgaagc tcacggcggg cgcatgtggg ccgagagcaa
     4741 cgcatcgggc ggggcgacct tccgcttcac cctcccggca gccgacgaga tgataggagg
     4801 tctagcatga cgaccaaggg acatatctac gtcatcgacg acgacgcggc gatgcgggat
     4861 tcgctgaatt tcctgctgga ttctgccggc ttcggcgtca cgctgtttga cgacgcgcaa
     4921 gcctttctcg acgccctgcc gggtctctcc ttcggctgcg tcgtctccga cgtgcgcatg
     4981 ccgggccttg acggcatcga gctgttgaag cggatgaagg cgcagcaaag cccctttccg
     5041 atcctcatca tgaccggtca cggcgacgtg ccgctcgcgg tcgaggcgat gaagttaggg
     5101 gcggtggact ttctggaaaa gcctttcgag gacgaccgcc tcaccgccat gatcgaatcg
     5161 gcgatccgcc aggccgagcc ggccgccaag agcgaggccg tcgcgcagga tatcgccgcc
     5221 cgcgtcgcct cgttgagccc cagggagcgc caggtcatgg aagggctgat cgccggcctt
     5281 tccaacaagc tgatcgcccg cgagtacgac atcagcccgc gcaccatcga ggtgtatcgg
     5341 gccaacgtca tgaccaagat gcaggccaac agcctttcgg agctggttcg cctcgcgatg
     5401 cgcgccggca tgctcaacga ttgacaattg atgtaagtta gctcactcat taggcaccgg
     5461 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     5521 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     5581 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     5641 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     5701 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccccac
     5761 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     5821 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     5881 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     5941 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     6001 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     6061 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     6121 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     6181 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
     6241 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     6301 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     6361 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     6541 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     6601 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     6661 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6721 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6781 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7201 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7261 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     7321 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7381 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7441 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7501 ttggtcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     7561 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga
     7621 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     7681 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     7741 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     7801 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     7861 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     7921 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     7981 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     8041 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     8101 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     8161 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     8221 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     8281 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     8341 gtttcatttg atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg
     8401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8461 ctaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8881 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca
//