LOCUS       pREDusk-AmpR-MCS        8087 bp ds-DNA     circular     14-JUL-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /label="T7 terminator_regulatory"
     CDS             complement(140..157)
                     /label="6xHis"
     misc_feature    179..237
                     /label="MCS"
     CDS             complement(205..237)
                     /label="T7 tag (gene 10 leader)"
     CDS             complement(241..258)
                     /label="thrombin site"
     CDS             complement(268..285)
                     /label="6xHis"
     RBS             304..326
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    complement(339..585)
                     /label="FixK2 Promoter"
     misc_feature    complement(655..674)
                     /label="pBRrevBam"
     promoter        880..957
                     /label="lacIq promoter"
     misc_feature    961..1563
                     /label="DrHO"
     misc_feature    1563..3080
                     /label="DrBphP (1-506)"
     misc_feature    3081..3128
                     /label="Linker"
     misc_feature    3081..3815
                     /label="BjFixL-HK"
     misc_feature    3815..4429
                     /label="FixJ"
     CDS             5233..5424
                     /label="rop"
     misc_feature    5526..5668
                     /label="bom"
     misc_feature    complement(5836..6518)
                     /label="ColE1 origin_rep_origin"
     promoter        6564..6668
                     /label="AmpR promoter"
     CDS             6669..7529
                     /label="AmpR"
     misc_feature    complement(7621..8076)
                     /label="M13 origin_rep_origin"
     misc_feature    complement(7753..8059)
                     /label="F1 origin_rep_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcAAGCTTgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatATG
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagact ccgttgtgat gacgcattgg
      361 tacgcggtat cgggaggttc gaaaatttcg agcgatatct taaggggggt gccttacgta
      421 gaaccccgta ggtcatgccc gaggccggtc ctggatggcg cggcggatac gcttgagcag
      481 gttttcgtcg agaagcggct tcaaaaccac gtcttttacg ccggcctcgg cggcccgggt
      541 cgagatgttt tcgtccggat agccggtgat caggatcacg ggcgtagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggtgcaaa
      901 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattgagggt ggtgaatgtg
      961 CTCCGACTCA TGATCATGAA GCTTTTACAA AGGGAGACCC TGGACCTGCA CCGCGAGGTC
     1021 GAGGCCCTGA TGCCCGTGAT GAGTGAGACG CTGACCCGGC GGGGCTATCT GGAGGTGCTG
     1081 CGGCGCCTGC AAGCGGTGGT CGCGCCGCTG GAAGCACAAC TCGACGCCCT GCCGCTGCCG
     1141 GACGAGCTGG CGTGGCCCGA GCGCCGCAAG GCCCATCTGC TCGCTCTGGA CCTTGCCGTA
     1201 ATGGGGGAAG GGCCGTCAAA TCAGCAGGCG CCACTGTCTG TGCCCGAGCT GACCACTCTG
     1261 GCGCAGGCCT TCGGAGCGCT TTACGTGCTT GAAGGCGCGA CGCTAGGCGG ACAGCTCATC
     1321 AGCCGCCATC TCAGGCGCAC GCTGGGGCTA AGCCCTGAGC AGGGCAGCGC CTATTTCTCC
     1381 GGCTATGGCC CCCAGACCGG CCCGCGCTGG CGCTCGTTCG GGGAAGTGCT GGAAGCCAGC
     1441 GTGCCGGCAG AAGACGCGGC AGAGGTCGTG GCGGGAGCGC GGCAGACCTT CGGGGCCTTC
     1501 CGCCGCGCCT TGCAGGGGCT GTCGGAAGCT GAAGCGGTCC AAGTGCCTGA GGTCGCCCAC
     1561 GCATGAgccg ggacccgttg cccttttttc caccgcttta ccttggtggc ccggaaatta
     1621 ccaccgagaa ctgcgagcgc gagccgattc atattcccgg cagcatccag ccgcacggcg
     1681 ccctgctcac tgccgacggg cacagcggcg aggtgctcca gatgagcctc aacgcggcca
     1741 cttttctggg acaggaaccc acagtgctgc gcggacagac cctcgccgca ctgctgcccg
     1801 agcagtggcc cgcgctgcaa gcggccctgc cccccggctg ccccgacgcc ctgcaatacc
     1861 gcgcaacgct ggactggcct gccgccgggc acctttcgct gacggtgcac cgggtcggcg
     1921 agttgctgat tctggaattc gagccgacgg aggcctggga cagcaccggg ccgcacgcgc
     1981 tgcgcaacgc gatgttcgcg ctcgaaagtg cccccaacct gcgggcgctg gccgaggtgg
     2041 cgacccagac ggtccgcgag ctgacgggct ttgaccgggt gatgctctac aaatttgccc
     2101 ccgacgccac cggcgaagtg attgccgagg cccgccgtga ggggctgcac gcctttctgg
     2161 gccaccgttt tcccgcgtcg gacattccgg cgcaggcccg cgcgctctac acccggcacc
     2221 tgctgcgcct gaccgccgac acccgcgccg ccgccgtgcc gctcgatccc gtcctcaacc
     2281 cgcagacgaa tgcgcccacc ccgctgggcg gcgccgtgct gcgcgccacc tcgcccatgc
     2341 acatgcagta cctgcggaac atgggcgtcg ggtcgagcct gtcggtgtcg gtggtggtcg
     2401 gcggccagct ctggggcctg atcgcctgcc accaccagac gccctacgtg ttgccgcccg
     2461 acctgcgaac cacgctcgaa tacctgggcc gcttgctgag cctgcaagtt caggtcaagg
     2521 aagcggcgga cgtggcggcc tttcgccaga gcctgcggga gcaccacgcg cgggtggccc
     2581 tcgcggcggc gcactcgctc tcgccgcacg acaccctcag tgacccggcg cttgacctgc
     2641 tgggcctgat gcgggccggg ggcctgattc tgcgtttcga gggccgctgg cagacgttgg
     2701 gtgaagtgcc gcctgccccg gcggtggacg cgctgctggc gtggctcgaa acccagccgg
     2761 gcgccctggt ccagaccgac gcgctAggcc aactgtggcc cgccggcgcc gatctcgccc
     2821 ccagcgcagc gggcctgctc gccatcagcg tgggcgaggg ctggtcggag tgcctcgtct
     2881 ggctgcggcc cgaactgcgg ctggaggtcg cctggggcgg ggccactcct gaccaggcga
     2941 aagacgacct cgggccgcgc cactcattcg acacctacct cgaagaaaaa cgcggctacg
     3001 ccgagccctg gcatcccggc gaaatcgagg aggcgcagga tctacgtgac acattgaccg
     3061 gggcgctggg cgagcgcctg ctccaggaac tgcaatccga gctcgtccac gtctccaggc
     3121 tgagcgccat gggcgaaatg gcgtccgcgc tcgcgcacga gctcaaccag ccgctggcgg
     3181 cgatcagcaa ctacatgaag ggctcgcggc ggctgcttgc cggcagcagt gatccgaaca
     3241 caccgaaggt cgaaagcgcc ctggaccgcg ccgccgagca ggcgctgcgc gccggccaga
     3301 tcatccggcg cctgcgcgac ttcgttgccc gcggcgaatc ggagaagcgg gtcgagagtc
     3361 tctccaagct gatcgaggag gccggcgcgc tcgggcttgc cggcgcgcgc gagcagaacg
     3421 tgcagctccg cttcagtctc gatccgggcg ccgatctcgt tctcgccgac cgggtgcaga
     3481 tccagcaggt cctggtcaac ctgttccgca acgcgctgga agcgatggct cagtcgcagc
     3541 gacgcgagct cgtcgtcacc aacacccccg ccgccgacga catgatcgag gtcgaagtgt
     3601 ccgacaccgg cagcggtttc caggacgacg tcattccgaa cctgtttcag actttcttca
     3661 ccaccaagga caccggcatg ggcgtgggac tgtccatcag ccgctcgatc atcgaagctc
     3721 acggcgggcg catgtgggcc gagagcaacg catcgggcgg ggcgaccttc cgcttcaccc
     3781 tcccggcagc cgacgagatg ataggaggtc tagcatgacg accaagggac atatctacgt
     3841 catcgacgac gacgcggcga tgcgggattc gctgaatttc ctgctggatt ctgccggctt
     3901 cggcgtcacg ctgtttgacg acgcgcaagc ctttctcgac gccctgccgg gtctctcctt
     3961 cggctgcgtc gtctccgacg tgcgcatgcc gggccttgac ggcatcgagc tgttgaagcg
     4021 gatgaaggcg cagcaaagcc cctttccgat cctcatcatg accggtcacg gcgacgtgcc
     4081 gctcgcggtc gaggcgatga agttaggggc ggtggacttt ctggaaaagc ctttcgagga
     4141 cgaccgcctc accgccatga tcgaatcggc gatccgccag gccgagccgg ccgccaagag
     4201 cgaggccgtc gcgcaggata tcgccgcccg cgtcgcctcg ttgagcccca gggagcgcca
     4261 ggtcatggaa gggctgatcg ccggcctttc caacaagctg atcgcccgcg agtacgacat
     4321 cagcccgcgc accatcgagg tgtatcgggc caacgtcatg accaagatgc aggccaacag
     4381 cctttcggag ctggttcgcc tcgcgatgcg cgccggcatg ctcaacgatt gacaattgat
     4441 gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac
     4501 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     4561 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     4621 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     4681 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     4741 gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     4801 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     4861 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     4921 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     4981 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     5041 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     5101 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     5161 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     5221 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     5281 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     5341 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     5401 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5461 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5521 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     5581 cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag
     5641 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     5701 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5761 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5821 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5881 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5941 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6001 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6061 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6121 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6181 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6241 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6301 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6361 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6421 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6481 gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca
     6541 taaacagtaa tacaaggggt gttCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
     6601 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
     6661 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
     6721 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
     6781 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
     6841 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
     6901 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
     6961 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
     7021 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
     7081 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
     7141 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
     7201 CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
     7261 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
     7321 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
     7381 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
     7441 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
     7501 TAGGTGCCTC ACTGATTAAG CATTGGTAAg aattaattca tgagcggata catatttgaa
     7561 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7621 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     7681 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     7741 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     7801 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     7861 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     7921 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     7981 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8041 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//