LOCUS       pREDawn-AmpR-MCS        9080 bp ds-DNA     circular     14-JUL-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /label="T7 terminator_regulatory"
     CDS             complement(140..157)
                     /label="6xHis"
     misc_feature    179..237
                     /label="MCS"
     CDS             complement(205..237)
                     /label="T7 tag (gene 10 leader)"
     CDS             complement(241..258)
                     /label="thrombin site"
     CDS             complement(268..285)
                     /label="6xHis"
     RBS             304..326
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      402..429
                     /label="T7Te terminator"
     terminator      445..516
                     /label="rrnB T1 terminator"
     CDS             complement(564..596)
                     /label="ssrA tag (LVA)"
     CDS             complement(597..1307)
                     /label="-lambda repressor"
     RBS             1314..1325
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
     misc_feature    complement(1332..1578)
                     /label="FixK2-promoter regulatory"
     misc_feature    complement(1648..1667)
                     /label="pBRrevBam"
     promoter        1873..1950
                     /label="lacIq promoter"
     misc_feature    1954..2556
                     /label="DrHO"
     misc_feature    2556..4073
                     /label="DrBphP (1-506)"
     misc_feature    4074..4121
                     /label="Linker"
     misc_feature    4074..4808
                     /label="BjFixL-HK"
     misc_feature    4808..5422
                     /label="FixJ"
     CDS             6226..6417
                     /label="rop"
     misc_feature    6519..6661
                     /label="bom"
     misc_feature    complement(6829..7511)
                     /label="ColE1 origin_rep_origin"
     promoter        7557..7661
                     /label="AmpR promoter"
     CDS             7662..8522
                     /label="AmpR"
     rep_origin      complement(8614..9069)
                     /label="M13 origin_rep_origin"
     misc_feature    complement(8746..9052)
                     /label="F1 origin_rep_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcAAGCTTgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatATG
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagagc aaccattatc accgccagag
      361 gtaaaatagt caacacgcac ggtgttactc tagtatataa acgcagaaag gcccacccga
      421 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
      481 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag tagcgatcta
      541 cactagcact atcagcgtta ttaagctact aaagcgtagt tttcgtcgtt tgcagcgcca
      601 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
      661 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
      721 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
      781 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct cggcttggag
      841 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
      901 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt
      961 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
     1021 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
     1081 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
     1141 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
     1201 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
     1261 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatcta gtatttctcc
     1321 tcttttctag actccgttgt gatgacgcat tggtacgcgg tatcgggagg ttcgaaaatt
     1381 tcgagcgata tcttaagggg ggtgccttac gtagaacccc gtaggtcatg cccgaggccg
     1441 gtcctggatg gcgcggcgga tacgcttgag caggttttcg tcgagaagcg gcttcaaaac
     1501 cacgtctttt acgccggcct cggcggcccg ggtcgagatg ttttcgtccg gatagccggt
     1561 gatcaggatc acgggcgtag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
     1621 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     1681 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     1741 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     1801 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     1861 gtcgagatcc cggacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
     1921 ccggaagaga gtcaattgag ggtggtgaat gtgCTCCGAC TCATGATCAT GAAGCTTTTA
     1981 CAAAGGGAGA CCCTGGACCT GCACCGCGAG GTCGAGGCCC TGATGCCCGT GATGAGTGAG
     2041 ACGCTGACCC GGCGGGGCTA TCTGGAGGTG CTGCGGCGCC TGCAAGCGGT GGTCGCGCCG
     2101 CTGGAAGCAC AACTCGACGC CCTGCCGCTG CCGGACGAGC TGGCGTGGCC CGAGCGCCGC
     2161 AAGGCCCATC TGCTCGCTCT GGACCTTGCC GTAATGGGGG AAGGGCCGTC AAATCAGCAG
     2221 GCGCCACTGT CTGTGCCCGA GCTGACCACT CTGGCGCAGG CCTTCGGAGC GCTTTACGTG
     2281 CTTGAAGGCG CGACGCTAGG CGGACAGCTC ATCAGCCGCC ATCTCAGGCG CACGCTGGGG
     2341 CTAAGCCCTG AGCAGGGCAG CGCCTATTTC TCCGGCTATG GCCCCCAGAC CGGCCCGCGC
     2401 TGGCGCTCGT TCGGGGAAGT GCTGGAAGCC AGCGTGCCGG CAGAAGACGC GGCAGAGGTC
     2461 GTGGCGGGAG CGCGGCAGAC CTTCGGGGCC TTCCGCCGCG CCTTGCAGGG GCTGTCGGAA
     2521 GCTGAAGCGG TCCAAGTGCC TGAGGTCGCC CACGCATGAg ccgggacccg ttgccctttt
     2581 ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag cgcgagccga
     2641 ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac gggcacagcg
     2701 gcgaggtgct ccagatgagc ctcaacgcgg ccacttttct gggacaggaa cccacagtgc
     2761 tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg caagcggccc
     2821 tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg cctgccgccg
     2881 ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggaa ttcgagccga
     2941 cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc gcgctcgaaa
     3001 gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc gagctgacgg
     3061 gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa gtgattgccg
     3121 aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg tcggacattc
     3181 cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc gacacccgcg
     3241 ccgccgccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc accccgctgg
     3301 gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg aacatgggcg
     3361 tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc ctgatcgcct
     3421 gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc gaatacctgg
     3481 gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg gcctttcgcc
     3541 agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg ctctcgccgc
     3601 acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc gggggcctga
     3661 ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc ccggcggtgg
     3721 acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc gacgcgctAg
     3781 gccaactgtg gcccgccggc gccgatctcg cccccagcgc agcgggcctg ctcgccatca
     3841 gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg cggctggagg
     3901 tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg cgccactcat
     3961 tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc ggcgaaatcg
     4021 aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgagcgc ctgctccagg
     4081 aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa atggcgtccg
     4141 cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg aagggctcgc
     4201 ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc gccctggacc
     4261 gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc gacttcgttg
     4321 cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag gaggccggcg
     4381 cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt ctcgatccgg
     4441 gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc aacctgttcc
     4501 gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc accaacaccc
     4561 ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt ttccaggacg
     4621 acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc atgggcgtgg
     4681 gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg gccgagagca
     4741 acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag atgataggag
     4801 gtctagcatg acgaccaagg gacatatcta cgtcatcgac gacgacgcgg cgatgcggga
     4861 ttcgctgaat ttcctgctgg attctgccgg cttcggcgtc acgctgtttg acgacgcgca
     4921 agcctttctc gacgccctgc cgggtctctc cttcggctgc gtcgtctccg acgtgcgcat
     4981 gccgggcctt gacggcatcg agctgttgaa gcggatgaag gcgcagcaaa gcccctttcc
     5041 gatcctcatc atgaccggtc acggcgacgt gccgctcgcg gtcgaggcga tgaagttagg
     5101 ggcggtggac tttctggaaa agcctttcga ggacgaccgc ctcaccgcca tgatcgaatc
     5161 ggcgatccgc caggccgagc cggccgccaa gagcgaggcc gtcgcgcagg atatcgccgc
     5221 ccgcgtcgcc tcgttgagcc ccagggagcg ccaggtcatg gaagggctga tcgccggcct
     5281 ttccaacaag ctgatcgccc gcgagtacga catcagcccg cgcaccatcg aggtgtatcg
     5341 ggccaacgtc atgaccaaga tgcaggccaa cagcctttcg gagctggttc gcctcgcgat
     5401 gcgcgccggc atgctcaacg attgacaatt gatgtaagtt agctcactca ttaggcaccg
     5461 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     5521 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     5581 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5641 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     5701 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca
     5761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     5821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     5881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     5941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     6001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     6061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     6121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     6181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     6241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     6301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     6361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     6541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     6601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7501 tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttCGCG
     7561 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     7621 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     7681 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     7741 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     7801 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     7861 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     7921 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     7981 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     8041 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     8101 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     8161 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA
     8221 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     8281 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     8341 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
     8401 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     8461 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     8521 AAgaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aaacgttaat attttgttaa
     8641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     8701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     8761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     8821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     9001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     9061 agggcgcgtc ccattcgcca
//