LOCUS       pREDawn-StrR-MCS        8453 bp ds-DNA     circular     14-JUL-2022
DEFINITION  .
KEYWORDS    "marker:SmR"                                                       
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /label="T7 terminator_regulatory"
     CDS             complement(140..157)
                     /label="6xHis"
     misc_feature    179..237
                     /label="MCS"
     CDS             complement(205..237)
                     /label="T7 tag (gene 10 leader)"
     CDS             complement(241..258)
                     /label="thrombin site"
     CDS             complement(268..285)
                     /label="6xHis"
     RBS             304..326
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      402..429
                     /label="T7Te terminator"
     terminator      445..516
                     /label="rrnB T1 terminator"
     CDS             complement(564..596)
                     /label="ssrA tag (LVA)"
     CDS             complement(597..1307)
                     /label="-lambda repressor"
     RBS             1314..1325
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
     misc_feature    complement(1332..1578)
                     /label="FixK2-promoter regulatory"
     misc_feature    complement(1648..1667)
                     /label="pBRrevBam"
     promoter        1873..1950
                     /label="lacIq promoter"
     misc_feature    1954..2556
                     /label="DrHO"
     misc_feature    2556..4073
                     /label="DrBphP (1-506)"
     misc_feature    4074..4121
                     /label="Linker"
     misc_feature    4074..4808
                     /label="BjFixL-HK"
     misc_feature    4808..5422
                     /label="FixJ"
     rep_origin      6226..6964
                     /label="CloDF13 ori"
     promoter        7012..7103
                     /label="AmpR promoter"
     CDS             7104..7895
                     /label="StrepR"
     misc_feature    complement(7987..8442)
                     /label="M13 origin_rep_origin"
     misc_feature    complement(8119..8425)
                     /label="F1 origin_rep_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcAAGCTTgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatATG
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagagc aaccattatc accgccagag
      361 gtaaaatagt caacacgcac ggtgttactc tagtatataa acgcagaaag gcccacccga
      421 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
      481 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag tagcgatcta
      541 cactagcact atcagcgtta ttaagctact aaagcgtagt tttcgtcgtt tgcagcgcca
      601 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
      661 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
      721 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
      781 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct cggcttggag
      841 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
      901 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt
      961 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
     1021 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
     1081 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
     1141 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
     1201 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
     1261 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatcta gtatttctcc
     1321 tcttttctag actccgttgt gatgacgcat tggtacgcgg tatcgggagg ttcgaaaatt
     1381 tcgagcgata tcttaagggg ggtgccttac gtagaacccc gtaggtcatg cccgaggccg
     1441 gtcctggatg gcgcggcgga tacgcttgag caggttttcg tcgagaagcg gcttcaaaac
     1501 cacgtctttt acgccggcct cggcggcccg ggtcgagatg ttttcgtccg gatagccggt
     1561 gatcaggatc acgggcgtag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
     1621 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     1681 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     1741 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     1801 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     1861 gtcgagatcc cggacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
     1921 ccggaagaga gtcaattgag ggtggtgaat gtgCTCCGAC TCATGATCAT GAAGCTTTTA
     1981 CAAAGGGAGA CCCTGGACCT GCACCGCGAG GTCGAGGCCC TGATGCCCGT GATGAGTGAG
     2041 ACGCTGACCC GGCGGGGCTA TCTGGAGGTG CTGCGGCGCC TGCAAGCGGT GGTCGCGCCG
     2101 CTGGAAGCAC AACTCGACGC CCTGCCGCTG CCGGACGAGC TGGCGTGGCC CGAGCGCCGC
     2161 AAGGCCCATC TGCTCGCTCT GGACCTTGCC GTAATGGGGG AAGGGCCGTC AAATCAGCAG
     2221 GCGCCACTGT CTGTGCCCGA GCTGACCACT CTGGCGCAGG CCTTCGGAGC GCTTTACGTG
     2281 CTTGAAGGCG CGACGCTAGG CGGACAGCTC ATCAGCCGCC ATCTCAGGCG CACGCTGGGG
     2341 CTAAGCCCTG AGCAGGGCAG CGCCTATTTC TCCGGCTATG GCCCCCAGAC CGGCCCGCGC
     2401 TGGCGCTCGT TCGGGGAAGT GCTGGAAGCC AGCGTGCCGG CAGAAGACGC GGCAGAGGTC
     2461 GTGGCGGGAG CGCGGCAGAC CTTCGGGGCC TTCCGCCGCG CCTTGCAGGG GCTGTCGGAA
     2521 GCTGAAGCGG TCCAAGTGCC TGAGGTCGCC CACGCATGAg ccgggacccg ttgccctttt
     2581 ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag cgcgagccga
     2641 ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac gggcacagcg
     2701 gcgaggtgct ccagatgagc ctcaacgcgg ccacttttct gggacaggaa cccacagtgc
     2761 tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg caagcggccc
     2821 tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg cctgccgccg
     2881 ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggaa ttcgagccga
     2941 cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc gcgctcgaaa
     3001 gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc gagctgacgg
     3061 gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa gtgattgccg
     3121 aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg tcggacattc
     3181 cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc gacacccgcg
     3241 ccgccgccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc accccgctgg
     3301 gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg aacatgggcg
     3361 tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc ctgatcgcct
     3421 gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc gaatacctgg
     3481 gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg gcctttcgcc
     3541 agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg ctctcgccgc
     3601 acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc gggggcctga
     3661 ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc ccggcggtgg
     3721 acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc gacgcgctAg
     3781 gccaactgtg gcccgccggc gccgatctcg cccccagcgc agcgggcctg ctcgccatca
     3841 gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg cggctggagg
     3901 tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg cgccactcat
     3961 tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc ggcgaaatcg
     4021 aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgagcgc ctgctccagg
     4081 aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa atggcgtccg
     4141 cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg aagggctcgc
     4201 ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc gccctggacc
     4261 gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc gacttcgttg
     4321 cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag gaggccggcg
     4381 cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt ctcgatccgg
     4441 gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc aacctgttcc
     4501 gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc accaacaccc
     4561 ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt ttccaggacg
     4621 acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc atgggcgtgg
     4681 gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg gccgagagca
     4741 acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag atgataggag
     4801 gtctagcatg acgaccaagg gacatatcta cgtcatcgac gacgacgcgg cgatgcggga
     4861 ttcgctgaat ttcctgctgg attctgccgg cttcggcgtc acgctgtttg acgacgcgca
     4921 agcctttctc gacgccctgc cgggtctctc cttcggctgc gtcgtctccg acgtgcgcat
     4981 gccgggcctt gacggcatcg agctgttgaa gcggatgaag gcgcagcaaa gcccctttcc
     5041 gatcctcatc atgaccggtc acggcgacgt gccgctcgcg gtcgaggcga tgaagttagg
     5101 ggcggtggac tttctggaaa agcctttcga ggacgaccgc ctcaccgcca tgatcgaatc
     5161 ggcgatccgc caggccgagc cggccgccaa gagcgaggcc gtcgcgcagg atatcgccgc
     5221 ccgcgtcgcc tcgttgagcc ccagggagcg ccaggtcatg gaagggctga tcgccggcct
     5281 ttccaacaag ctgatcgccc gcgagtacga catcagcccg cgcaccatcg aggtgtatcg
     5341 ggccaacgtc atgaccaaga tgcaggccaa cagcctttcg gagctggttc gcctcgcgat
     5401 gcgcgccggc atgctcaacg attgacaatt gatgtaagtt agctcactca ttaggcaccg
     5461 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     5521 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     5581 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5641 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     5701 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca
     5761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     5821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     5881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     5941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     6001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     6061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     6121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     6181 atcattaccc ccatgaacag aaatccccct tacacggagg catcaGATCA AAGGATCTTC
     6241 TTGAGATCCT TTTTTTCTGC GCGTAATCTT TTGCCCTGTA AACGAAAAAA CCACCTGGGG
     6301 AGGTGGTTTG ATCGAAGGTT AAGTCAGTTG GGGAACTGCT TAACCGTGGT AACTGGCTTT
     6361 CGCAGAGCAC AGCAACCAAA TCTGTCCTTC CAGTGTAGCC GGACTTTGGC GCACACTTCA
     6421 AGAGCAACCG CGTGTTTAGC TAAACAAATC CTCTGCGAAC TCCCAGTTAC CAATGGCTGC
     6481 TGCCAGTGGC GTTTTACCGT GCTTTTCCGG GTTGGACTCA AGTGAACAGT TACCGGATAA
     6541 GGCGCAGCAG TCGGGCTGAA CGGGGAGTTC TTGCTTACAG CCCAGCTTGG AGCGAACGAC
     6601 CTACACCGAG CCGAGATACC AGTGTGTGAG CTATGAGAAA GCGCCACACT TCCCGTAAGG
     6661 GAGAAAGGCG GAACAGGTAT CCGGTAAACG GCAGGGTCGG AACAGGAGAG CGCAAGAGGG
     6721 AGCGACCCGC CGGAAACGGT GGGGATCTTT AAGTCCTGTC GGGTTTCGCC CGTACTGTCA
     6781 GATTCATGGT TGAGCCTCAC GGCTCCCACA GATGCACCGG AAAAGCGTCT GTTTATGTGA
     6841 ACTCTGGCAG GAGGGCGGAG CCTATGGAAA AACGCCACCG GCGCGGCCCT GCTGTTTTGC
     6901 CTCACATGTT AGTCCCCTGC TTATCCACGG AATCTGTGGG TAACTTTGTA TGTGTCCGCA
     6961 GCGCCCGCCG CAGTCTCACG CCCGGAGCGT AGCGACCGAG TGAGCTAGCT ATTTGTTTAT
     7021 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
     7081 AATAATATTG AAAAAGGAAG AGTATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC
     7141 TATCAGAGGT AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT
     7201 TGTACGGCTC CGCAGTGGAT GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA
     7261 CGGTGACCGT AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA
     7321 CTTCGGCTTC CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC
     7381 ACGACGACAT CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC
     7441 AGCGCAATGA CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA
     7501 TCTTGCTGAC AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC
     7561 TCTTTGATCC GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT
     7621 GGAACTCGCC GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA
     7681 TTTGGTACAG CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA
     7741 TGGAGCGCCT GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG
     7801 GACAAGAAGA AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG
     7861 TGAAAGGCGA GATCACCAAG GTAGTCGGCA AATAAgaatt aattcatgag cggatacata
     7921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7981 ccacctaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca
//