LOCUS       Exported File           7646 bp ds-DNA    circular SYN 15-JUL-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pSFS2A-CaHygB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 15, 2022 from SnapGene 1.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          2815..4924
                     /organism="Expression vector pYM70"
                     /lab_host="Candida albicans"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:754152"
     source          3473..3476
                     /organism="Expression vector pYM70"
                     /lab_host="Candida albicans"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:754152"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="f1 bacteriophage origin of replication"
                     /note="color: #b7e6d7"
     primer_bind     600..616
                     /label="common sequencing primer, one of multiple similar 
                     variants"
                     /note="common sequencing primer, one of multiple simil"
                     /note="color: #d6b295; direction: RIGHT"
     promoter        626..644
                     /label="promoter for bacteriophage T7 RNA polymerase"
                     /note="promoter for bacteriophage T7 RNA polymerase pr"
                     /note="color: #84b0dc"
     misc_feature    653..673
                     /label="polylinker"
                     /note="polylinker"
                     /note="color: #b4abac"
     misc_feature    674..707
                     /label="FLP recombination target sequence (FRT)"
                     /note="FLP recombination target sequence"
                     /note="color: #b4abac"
     misc_feature    712..1259
                     /label="from the Candida albicans MAL2 gene"
                     /note="from the Candida albicans MAL2 gene"
                     /note="color: #c6c9d1"
     CDS             1266..2537
                     /label="FLP"
                     /note="FLP"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2178..2180
                     /label="DNA binding site [nucleotide binding]"
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #b4abac"
     misc_feature    2178..2180
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2187..2192
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2187..2189
                     /label="DNA binding site [nucleotide binding]"
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #b4abac"
     misc_feature    2202..2204
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2211..2216
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2226..2228
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2238..2240
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2253..2255
                     /label="DNA binding site [nucleotide binding]"
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #b4abac"
     misc_feature    2253..2255
                     /label="active site"
                     /note="active site"
                     /note="color: #b4abac"
     misc_feature    2256..2258
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2292..2294
                     /label="active site"
                     /label="active site"
                     /note="active site"
                     /note="color: #b4abac"
     misc_feature    2292..2294
                     /label="DNA binding site [nucleotide binding]"
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #b4abac"
     misc_feature    2295..2297
                     /label="miscellaneous"
                     /note="color: #b4abac"
     misc_feature    2298..2300
                     /label="active site"
                     /note="active site"
                     /note="color: #b4abac"
     misc_feature    2298..2300
                     /label="DNA binding site [nucleotide binding]"
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #b4abac"
     misc_feature    2538..2814
                     /label="from the Candida albicans ACT1 gene"
                     /note="from the Candida albicans ACT1 gene"
                     /note="color: #c6c9d1"
     misc_feature    2815..4924
                     /note="From pYM70"
                     /note="color: #00ff00"
     misc_feature    2827..3467
                     /note="Candida albicans TEF2 promoter"
                     /note="color: #a6acb3"
     misc_feature    3468..3477
                     /note="Former XhoI site - cut, klenow filled in, ligated"
                     /note="color: #a6acb3"
     gene            3478..4503
                     /gene="CaHygB"
                     /note="CaHygB"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3478..4503
                     /codon_start=1
                     /transl_table=11
                     /gene="CaHygB"
                     /product="hygromycin B phosphotransferase"
                     /note="CaHygB"
                     /note="synthetic; optimized for expression in Candida 
                     albicans"
                     /note="color: #993366"
                     /protein_id="ADF45590.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    3762
                     /note="T to G to remove a SacI site"
                     /note="color: #a6acb3"
     misc_feature    4510..4897
                     /note="Candida albicans ACT1 terminator"
                     /note="color: #a6acb3"
     misc_feature    4931..4964
                     /label="FLP recombination target sequence (FRT)"
                     /note="FLP recombination target sequence"
                     /note="color: #b4abac"
     misc_feature    4965..5006
                     /label="polylinker"
                     /note="polylinker"
                     /note="color: #b4abac"
     promoter        complement(5019..5037)
                     /label="promoter for bacteriophage T3 RNA polymerase"
                     /note="promoter for bacteriophage T3 RNA polymerase pr"
                     /note="color: #c7b0e3; direction: LEFT"
     primer_bind     complement(5058..5074)
                     /label="common sequencing primer, one of multiple similar 
                     variants"
                     /note="common sequencing primer, one of multiple simil"
                     /note="color: #c7b0e3; direction: LEFT"
     protein_bind    5082..5098
                     /label="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="The lac ryl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="The lac repressor binds to the lac operator to"
                     /note="color: #ffef86"
     promoter        complement(5106..5136)
                     /label="promoter for the E. coli lac operon"
                     /note="promoter for the E. coli lac operon promoter"
                     /note="color: #b1ff67; direction: LEFT"
     protein_bind    5151..5172
                     /label="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="CAP binding activates transcription in the pres"
                     /note="color: #d6b295"
     rep_origin      complement(5460..6048)
                     /direction=LEFT
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin o"
                     /note="color: #f8d3a9"
     promoter        6268..6370
                     /label="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
                     /note="promoter of the E. coli cat gene encoding chlor"
                     /note="color: #ffef86"
     CDS             6371..7030
                     /label="cat CDS"
                     /note="cat CDS"
                     /note="color: #75c6a9; direction: RIGHT"
     promoter        complement(7518..7623)
                     /label="bla promoter"
                     /note="bla promoter"
                     /note="color: #9eafd2; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggaagttc ctatactttc tagagaatag gaacttcgga tccaataatg
      721 attggtttga tatttttgtc tagtaccatc tgtaccatta cacttaaatt atctttatat
      781 ctgtctaact cgactgtctg gatttcattg atgtagtcgt atgcatcgtt agttccaaaa
      841 aatattgtca tcaatttgat attggtttcc gactctaaaa tttttggaag aatttgtcta
      901 gcgtgctctg agttgtagcc actgaaacca cggttaataa catccaattt tcggatatac
      961 acattctgta atgctggatg aaagccatac tgggtacaac taaactgggt gatggagtca
     1021 ccgaacaaca caaatttacc gtattccatg attgctatgg ttgagaattt tttttttttc
     1081 ttgtcccacg ccatttttca aattatgcag ttgagaatgt tagtttttgt gtacaccccg
     1141 ttcgctgaat atttcggaat aattcaaaga ttggggagtg ggggaggcga tagacgaaga
     1201 cacggtataa aaatgggcaa aattttcccc aactttttgc agtggtttaa ctaataatcg
     1261 tcgacatgcc acaatttgat atattatgta aaacaccacc taaggtgctt gttcgtcagt
     1321 ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc attatgtgct gctgaactaa
     1381 cctatttatg ttggatgatt acacataacg gaacagcaat caagagagcc acattcatga
     1441 gctataatac tatcataagc aattcgttga gtttcgatat tgtcaataaa tcactccagt
     1501 ttaaatacaa gacgcaaaaa gcaacaattt tggaagcctc attaaagaaa ttgattcctg
     1561 cttgggaatt tacaattatt ccttactatg gacaaaaaca tcaatctgat atcactgata
     1621 ttgtaagtag tttgcaatta cagttcgaat catcggaaga agcagataag ggaaatagcc
     1681 acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga aagcatctgg gagatcactg
     1741 agaaaatact aaattcgttt gagtatactt cgagatttac aaaaacaaaa actttatacc
     1801 aattcctctt cctagctact ttcatcaatt gtggaagatt cagcgatatt aagaacgttg
     1861 atccgaaatc atttaaatta gtccaaaata agtatttggg agtaataatc cagtgtttag
     1921 tgacagagac aaagacaagc gttagtaggc acatatactt ctttagcgca aggggtagga
     1981 tcgatccact tgtatatttg gatgaatttt tgaggaattc tgaaccagtc ctaaaacgag
     2041 taaataggac cggcaattct tcaagcaata aacaggaata ccaattatta aaagataact
     2101 tagtcagatc gtacaataaa gctttgaaga aaaatgcgcc ttattcaatc tttgctataa
     2161 aaaatggccc aaaatctcac attggaagac atttgatgac ctcatttctt tcaatgaagg
     2221 gcctaacgga gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg
     2281 ccaggacaac gtatactcat cagataacag caatacctga tcactacttc gcactagttt
     2341 ctcggtacta tgcatatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta
     2401 atccaattga ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac
     2461 gataccccgc atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca
     2521 taaatagacg catataagag tgaaattctg gaaatctgga aatctggttt tgtattcttg
     2581 ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa atctttgtat
     2641 attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta tttgagagat
     2701 tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa aaaaacaaaa
     2761 caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt ctatccacct
     2821 gacgtcgtat agtgcttgct gttcgatatt gctagaggca aagaaaaagg attacctcac
     2881 gcctaaaatg gaatttgaaa attgaggaga tgagttgagc ttttgtcttt tacactttcc
     2941 ctattcaatt gtgccgcaaa aacttcaata tctaacacag tgtgaaatat ttgttatgta
     3001 taccgaaagc caaataccat ttaaactggt atttcaagat ttcataaatc aagtttctgg
     3061 ttaaattaat tgctgttaaa cagaacaaga gcttcacatt gatgttggaa taactactat
     3121 agaattataa aatacacaaa accaggtcgt gtacgcaata acttttcaat gtatgaaaaa
     3181 attttttttt tttttttgct tcttctcttc gcttcttttt tctagttatc aatttggcaa
     3241 tgaattgatt tgattgccct ttgtttagtt tactatgaaa aaaaattttt tttttgtctc
     3301 tgtcagattt tctctcattt ttttttttgc ctcataatga acaactcaaa aaagaatata
     3361 aatttctcat caaagacctt tccaattgaa aattttttct ttccttcttt tccttttttt
     3421 ttttctttct ttcttctttt ctaagtatac attttatata atcaatcctc gatcgagatg
     3481 aaaaaaccag aattgactgc tacttctgtt gaaaaatttt tgattgaaaa atttgattct
     3541 gtttctgatt tgatgcaatt gtctgaaggt gaagaatcaa gagctttttc ttttgatgtt
     3601 ggtggtagag gttatgtttt gagagttaat tcttgtgctg atggttttta taaagataga
     3661 tatgtttata gacattttgc ttctgctgct ttgccaattc cagaagtttt ggatattggt
     3721 gaattttctg aatctttgac ttattgtatt tcaagaagag cgcaaggtgt tactttgcaa
     3781 gatttgccag aaactgaatt gccagctgtt ttgcaaccag ttgctgaagc tatggatgct
     3841 attgctgctg ctgatttgtc tcaaacttct ggttttggtc catttggtcc acaaggtatt
     3901 ggtcaatata ctacttggag agattttatt tgtgctattg ctgatccaca tgtttatcat
     3961 tggcaaactg ttatggatga tactgtttct gcttctgttg ctcaagcatt ggatgaattg
     4021 atgttgtggg ctgaagattg tccagaagtt agacatttgg ttcatgctga ttttggttct
     4081 aataatgttt tgactgataa tggtagaatt actgctgtta ttgattggtc tgaagctatg
     4141 tttggtgatt ctcaatatga agttgctaat attttttttt ggagaccatg gttggcttgt
     4201 atggaacaac aaactagata ttttgaaaga agacatccag aattggctgg ttctccaaga
     4261 ttgagagctt atatgttgag aattggtttg gatcaattgt atcaatcttt ggttgatggt
     4321 aattttgatg atgctgcttg ggctcaaggt agatgtgatg ctattgttag atcaggtgct
     4381 ggtactgttg gtagaactca aattgctaga agatcagctg ctgtttggac tgatggttgt
     4441 gttgaagttt tggctgattc tggtaataga agaccatcta ctagaccaag agctaaagaa
     4501 taaggatccg agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt
     4561 ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata
     4621 aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt
     4681 tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata
     4741 cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct tgttgttgtt
     4801 gttttcatct tctgtatcag aggaacttat tttattattt tcgtcacggg tattttctct
     4861 tgtttgatga ttcatcccat tcattccatc ataaaatgac gtctaagaaa ccattattat
     4921 catgctgcag gaagttccta tactttctag agaataggaa cttcagatcc actagttcta
     4981 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     5041 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5221 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5881 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6121 atgagattat caaaaaggat cttcacctag atccttttcg accgaataaa tacctgtgac
     6181 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     6241 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     6301 taatgaaata agatcactac cgggcgtatt ttttgagttg tcgagatttt caggagctaa
     6361 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     6421 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     6481 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     6541 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat
     6601 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     6661 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     6721 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     6781 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     6841 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     6901 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     6961 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     7021 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
     7081 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
     7141 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
     7201 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
     7261 ctccccgtgg aggtaataat tgacgatatg atcctttttt tctgatcaaa agtgctcatc
     7321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcaagggtta
     7561 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7621 gcgcacattt ccccgaaaag tgccac
//
