LOCUS       pBbA2c-TraI             3245 bp ds-DNA     circular     15-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     266..285
                     /label="CAT-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(352..454)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(484..1107)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1015..1034
                     /label="Tet-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1126..1181
                     /label="tetR/tetA promoters"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1162..1180
                     /label="tet operator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1191..1218)
                     /label="pBbA2c-RFP REV"
                     /note="sequence: GAAACTGTGAGTATCCGCATatgtatatctccttcttaaaagatcttt"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1219..1240
                     /label="TraI FWD"
                     /note="sequence: tttaagaaggagatatacatATGCGGATACTCACAGTTTCGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1219..1854
                     /label="TraI"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(1835..1854)
                     /label="TraI REV"
                     /note="sequence: ccttactcgagtttggatccTCACGCGGCACTCCTGATCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1855..1874
                     /label="pBbA2c-RFP FWD"
                     /note="sequence: CGATCAGGAGTGCCGCGTGAggatccaaactcgagtaagg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1887..1958
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      1974..2001
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2029..2046)
                     /label="L4440"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(2163..2708)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(2243..2262)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2822..2916
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2937..351)
                     /label="CmR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
       61 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      121 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      181 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      241 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      301 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      361 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      421 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac
      481 gtcttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      541 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      601 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      661 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      721 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      781 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      841 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      901 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      961 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     1021 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     1081 cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga cactctatcg
     1141 ttgatagagt tattttacca ctccctatca gtgatagaga aaagaattca aaagatcttt
     1201 taagaaggag atatacatAT GCGGATACTC ACAGTTTCGC CGGACCAATA CGAACGGTAT
     1261 CGAAGCTTCC TCAAACAGAT GCACCGCCTT CGCGCGACGG TGTTCGGTGG CCGTCTCGAA
     1321 TGGGACGTCT CCATCATCGC TGGGGAAGAG CGCGACCAAT ACGATAATTT CAAGCCGAGC
     1381 TACCTTCTGG CGATTACTGA CAGTGGGCGG GTCGCCGGAT GCGTCAGACT TCTTCCGGCT
     1441 TGCGGGCCTA CGATGCTGGA GCAGACTTTT TCGCAACTCC TCGAAATGGG CTCGCTCGCA
     1501 GCGCATTCCG GAATGGTAGA GAGCTCTCGC TTCTGCGTCG ACACCTCCCT CGTCTCACGG
     1561 AGAGATGCGA GCCAGCTGCA CCTGGCGACT CTTACCTTAT TCGCCGGTAT CATTGAATGG
     1621 TCGATGGCCA GCGGCTACAC GGAGATCGTC ACGGCGACCG ATCTTCGTTT CGAGCGCATC
     1681 CTGAAGCGTG CTGGATGGCC GATGCGCCGG CTTGGTGAAC CCACCGCGAT CGGCAACACC
     1741 ATTGCCATTG CCGGAAGACT GCCAGCCGAT CGTGCCAGCT TCGAGCAGGT TTGCCCTCCG
     1801 GGCTACTATT CTATCCCCCG GATCGACGTG GCAGCGATCA GGAGTGCCGC GTGAggatcc
     1861 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1921 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1981 cttcgggtgg gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga
     2041 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     2101 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     2161 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     2221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     2281 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     2341 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     2401 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2461 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     2521 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     2581 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     2641 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     2701 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     2761 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg
     2821 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2881 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     2941 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     3001 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     3061 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     3121 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     3181 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     3241 atgtg
//